Synechococcus phage S-PM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Nodensvirus; Synechococcus virus SPM2

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5GQM5|Q5GQM5_BPSYP Methylamine util OS=Synechococcus phage S-PM2 OX=238854 GN=S-PM2d113 PE=4 SV=1
MM1 pKa = 7.42MYY3 pKa = 10.48ILTLRR8 pKa = 11.84GHH10 pKa = 5.12EE11 pKa = 4.03TGVFSLVNDD20 pKa = 3.39IGEE23 pKa = 4.08QIIPIFEE30 pKa = 4.57EE31 pKa = 3.98YY32 pKa = 10.89DD33 pKa = 3.35DD34 pKa = 4.22AEE36 pKa = 4.95RR37 pKa = 11.84YY38 pKa = 9.92HH39 pKa = 9.09SMIADD44 pKa = 3.4QSDD47 pKa = 3.51DD48 pKa = 3.94GEE50 pKa = 4.94IPLEE54 pKa = 4.16ILDD57 pKa = 3.76IDD59 pKa = 3.9EE60 pKa = 4.72DD61 pKa = 4.89VIVSACTEE69 pKa = 3.97RR70 pKa = 11.84DD71 pKa = 3.02QKK73 pKa = 11.15YY74 pKa = 10.78AIITADD80 pKa = 4.4DD81 pKa = 4.25LLIPPDD87 pKa = 3.85NVILL91 pKa = 4.12

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GQI5|Q5GQI5_BPSYP Hypothetical-Protein / belonging to T4-LIKE GC: 846 OS=Synechococcus phage S-PM2 OX=238854 GN=S-PM2d152 PE=4 SV=1
MM1 pKa = 7.76NDD3 pKa = 3.23NQRR6 pKa = 11.84GALLSFAYY14 pKa = 10.55NLGAAFYY21 pKa = 10.64GGRR24 pKa = 11.84NFNTITRR31 pKa = 11.84ILRR34 pKa = 11.84DD35 pKa = 3.94QKK37 pKa = 8.59WHH39 pKa = 6.08EE40 pKa = 4.18VPKK43 pKa = 10.28VLEE46 pKa = 4.24MYY48 pKa = 10.09RR49 pKa = 11.84NPGTKK54 pKa = 9.64VEE56 pKa = 4.25AGLLRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 10.24AEE66 pKa = 3.84GKK68 pKa = 10.07LWMSS72 pKa = 4.16

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

243

0

243

60965

33

3779

250.9

28.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.985 ± 0.219

0.825 ± 0.084

6.737 ± 0.133

6.366 ± 0.229

4.544 ± 0.089

7.509 ± 0.283

1.353 ± 0.135

7.152 ± 0.16

5.807 ± 0.344

7.252 ± 0.125

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.899 ± 0.19

6.335 ± 0.202

3.945 ± 0.139

3.546 ± 0.092

4.14 ± 0.109

7.662 ± 0.261

7.055 ± 0.29

6.395 ± 0.158

1.094 ± 0.093

4.399 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski