Amino acid dipepetide frequency for Synechococcus phage S-PM2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.806AlaAla: 4.806 ± 0.53
0.525AlaCys: 0.525 ± 0.117
3.576AlaAsp: 3.576 ± 0.309
3.559AlaGlu: 3.559 ± 0.275
2.46AlaPhe: 2.46 ± 0.211
4.773AlaGly: 4.773 ± 0.418
0.673AlaHis: 0.673 ± 0.113
3.789AlaIle: 3.789 ± 0.326
3.313AlaLys: 3.313 ± 0.278
4.298AlaLeu: 4.298 ± 0.331
1.017AlaMet: 1.017 ± 0.15
3.609AlaAsn: 3.609 ± 0.307
2.411AlaPro: 2.411 ± 0.26
2.198AlaGln: 2.198 ± 0.238
2.592AlaArg: 2.592 ± 0.209
5.085AlaSer: 5.085 ± 0.359
4.626AlaThr: 4.626 ± 0.403
3.625AlaVal: 3.625 ± 0.253
0.508AlaTrp: 0.508 ± 0.081
2.411AlaTyr: 2.411 ± 0.223
0.0AlaXaa: 0.0 ± 0.0
Cys
0.525CysAla: 0.525 ± 0.09
0.098CysCys: 0.098 ± 0.045
0.689CysAsp: 0.689 ± 0.131
0.623CysGlu: 0.623 ± 0.132
0.295CysPhe: 0.295 ± 0.074
0.492CysGly: 0.492 ± 0.102
0.18CysHis: 0.18 ± 0.056
0.673CysIle: 0.673 ± 0.128
0.574CysLys: 0.574 ± 0.101
0.623CysLeu: 0.623 ± 0.114
0.246CysMet: 0.246 ± 0.07
0.508CysAsn: 0.508 ± 0.095
0.476CysPro: 0.476 ± 0.107
0.328CysGln: 0.328 ± 0.083
0.279CysArg: 0.279 ± 0.078
0.492CysSer: 0.492 ± 0.097
0.197CysThr: 0.197 ± 0.06
0.525CysVal: 0.525 ± 0.093
0.082CysTrp: 0.082 ± 0.041
0.344CysTyr: 0.344 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
4.314AspAla: 4.314 ± 0.384
0.541AspCys: 0.541 ± 0.112
4.757AspAsp: 4.757 ± 0.394
4.33AspGlu: 4.33 ± 0.31
3.281AspPhe: 3.281 ± 0.189
5.479AspGly: 5.479 ± 0.439
0.935AspHis: 0.935 ± 0.129
4.839AspIle: 4.839 ± 0.278
3.231AspLys: 3.231 ± 0.334
4.79AspLeu: 4.79 ± 0.279
1.509AspMet: 1.509 ± 0.235
4.134AspAsn: 4.134 ± 0.297
3.313AspPro: 3.313 ± 0.348
2.329AspGln: 2.329 ± 0.175
2.707AspArg: 2.707 ± 0.231
4.38AspSer: 4.38 ± 0.319
4.035AspThr: 4.035 ± 0.304
4.33AspVal: 4.33 ± 0.241
0.984AspTrp: 0.984 ± 0.156
3.149AspTyr: 3.149 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
3.1GluAla: 3.1 ± 0.29
0.558GluCys: 0.558 ± 0.094
4.101GluAsp: 4.101 ± 0.279
4.921GluGlu: 4.921 ± 0.493
3.149GluPhe: 3.149 ± 0.265
3.822GluGly: 3.822 ± 0.24
0.968GluHis: 0.968 ± 0.15
5.315GluIle: 5.315 ± 0.383
4.166GluLys: 4.166 ± 0.457
5.364GluLeu: 5.364 ± 0.377
1.394GluMet: 1.394 ± 0.212
3.855GluAsn: 3.855 ± 0.252
1.558GluPro: 1.558 ± 0.138
2.428GluGln: 2.428 ± 0.259
2.575GluArg: 2.575 ± 0.275
3.789GluSer: 3.789 ± 0.215
4.183GluThr: 4.183 ± 0.272
4.396GluVal: 4.396 ± 0.236
0.837GluTrp: 0.837 ± 0.156
3.182GluTyr: 3.182 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
2.296PheAla: 2.296 ± 0.195
0.591PheCys: 0.591 ± 0.1
3.855PheAsp: 3.855 ± 0.265
3.133PheGlu: 3.133 ± 0.254
1.542PhePhe: 1.542 ± 0.169
2.969PheGly: 2.969 ± 0.217
0.656PheHis: 0.656 ± 0.141
2.903PheIle: 2.903 ± 0.255
2.772PheLys: 2.772 ± 0.241
3.067PheLeu: 3.067 ± 0.24
0.804PheMet: 0.804 ± 0.159
2.871PheAsn: 2.871 ± 0.279
1.804PhePro: 1.804 ± 0.182
1.394PheGln: 1.394 ± 0.172
1.821PheArg: 1.821 ± 0.208
3.576PheSer: 3.576 ± 0.273
3.363PheThr: 3.363 ± 0.213
3.379PheVal: 3.379 ± 0.259
0.476PheTrp: 0.476 ± 0.098
2.165PheTyr: 2.165 ± 0.183
0.0PheXaa: 0.0 ± 0.0
Gly
4.15GlyAla: 4.15 ± 0.435
0.591GlyCys: 0.591 ± 0.099
5.2GlyAsp: 5.2 ± 0.442
4.921GlyGlu: 4.921 ± 0.33
2.985GlyPhe: 2.985 ± 0.252
7.808GlyGly: 7.808 ± 0.783
0.869GlyHis: 0.869 ± 0.144
4.741GlyIle: 4.741 ± 0.255
3.592GlyLys: 3.592 ± 0.328
4.199GlyLeu: 4.199 ± 0.309
1.181GlyMet: 1.181 ± 0.174
5.397GlyAsn: 5.397 ± 0.505
1.985GlyPro: 1.985 ± 0.247
2.542GlyGln: 2.542 ± 0.24
2.723GlyArg: 2.723 ± 0.225
7.168GlySer: 7.168 ± 0.542
6.364GlyThr: 6.364 ± 0.571
4.757GlyVal: 4.757 ± 0.367
0.886GlyTrp: 0.886 ± 0.163
3.035GlyTyr: 3.035 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
0.673HisAla: 0.673 ± 0.128
0.131HisCys: 0.131 ± 0.056
0.787HisAsp: 0.787 ± 0.16
0.902HisGlu: 0.902 ± 0.15
0.853HisPhe: 0.853 ± 0.119
0.853HisGly: 0.853 ± 0.142
0.361HisHis: 0.361 ± 0.083
0.837HisIle: 0.837 ± 0.123
1.033HisLys: 1.033 ± 0.156
0.919HisLeu: 0.919 ± 0.163
0.328HisMet: 0.328 ± 0.081
0.722HisAsn: 0.722 ± 0.113
0.541HisPro: 0.541 ± 0.089
0.476HisGln: 0.476 ± 0.094
0.591HisArg: 0.591 ± 0.085
0.771HisSer: 0.771 ± 0.12
0.804HisThr: 0.804 ± 0.124
0.755HisVal: 0.755 ± 0.142
0.23HisTrp: 0.23 ± 0.071
0.968HisTyr: 0.968 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
4.298IleAla: 4.298 ± 0.313
0.558IleCys: 0.558 ± 0.111
5.643IleAsp: 5.643 ± 0.327
4.839IleGlu: 4.839 ± 0.263
2.46IlePhe: 2.46 ± 0.191
4.741IleGly: 4.741 ± 0.36
0.705IleHis: 0.705 ± 0.127
5.101IleIle: 5.101 ± 0.334
4.462IleLys: 4.462 ± 0.382
4.987IleLeu: 4.987 ± 0.242
1.247IleMet: 1.247 ± 0.188
4.347IleAsn: 4.347 ± 0.273
3.002IlePro: 3.002 ± 0.223
2.92IleGln: 2.92 ± 0.21
3.248IleArg: 3.248 ± 0.247
5.971IleSer: 5.971 ± 0.493
5.413IleThr: 5.413 ± 0.42
4.724IleVal: 4.724 ± 0.283
0.426IleTrp: 0.426 ± 0.09
2.428IleTyr: 2.428 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
3.149LysAla: 3.149 ± 0.309
0.476LysCys: 0.476 ± 0.09
3.182LysAsp: 3.182 ± 0.3
4.462LysGlu: 4.462 ± 0.489
2.789LysPhe: 2.789 ± 0.259
3.133LysGly: 3.133 ± 0.274
1.001LysHis: 1.001 ± 0.143
4.478LysIle: 4.478 ± 0.353
4.56LysLys: 4.56 ± 0.656
4.068LysLeu: 4.068 ± 0.399
1.608LysMet: 1.608 ± 0.261
3.609LysAsn: 3.609 ± 0.363
2.1LysPro: 2.1 ± 0.22
2.329LysGln: 2.329 ± 0.255
2.707LysArg: 2.707 ± 0.23
3.609LysSer: 3.609 ± 0.325
3.412LysThr: 3.412 ± 0.218
3.789LysVal: 3.789 ± 0.263
0.722LysTrp: 0.722 ± 0.146
2.887LysTyr: 2.887 ± 0.295
0.0LysXaa: 0.0 ± 0.0
Leu
4.281LeuAla: 4.281 ± 0.281
0.656LeuCys: 0.656 ± 0.145
5.265LeuAsp: 5.265 ± 0.391
4.741LeuGlu: 4.741 ± 0.295
3.149LeuPhe: 3.149 ± 0.236
4.544LeuGly: 4.544 ± 0.263
1.378LeuHis: 1.378 ± 0.164
4.741LeuIle: 4.741 ± 0.233
4.38LeuLys: 4.38 ± 0.427
5.003LeuLeu: 5.003 ± 0.379
1.493LeuMet: 1.493 ± 0.188
5.183LeuAsn: 5.183 ± 0.303
2.969LeuPro: 2.969 ± 0.204
2.624LeuGln: 2.624 ± 0.206
3.412LeuArg: 3.412 ± 0.233
5.79LeuSer: 5.79 ± 0.312
4.97LeuThr: 4.97 ± 0.437
4.412LeuVal: 4.412 ± 0.312
0.574LeuTrp: 0.574 ± 0.118
2.953LeuTyr: 2.953 ± 0.258
0.0LeuXaa: 0.0 ± 0.0
Met
1.296MetAla: 1.296 ± 0.237
0.148MetCys: 0.148 ± 0.05
1.115MetAsp: 1.115 ± 0.198
0.919MetGlu: 0.919 ± 0.153
0.82MetPhe: 0.82 ± 0.171
0.968MetGly: 0.968 ± 0.151
0.328MetHis: 0.328 ± 0.099
1.247MetIle: 1.247 ± 0.171
1.46MetLys: 1.46 ± 0.211
1.443MetLeu: 1.443 ± 0.244
0.558MetMet: 0.558 ± 0.104
1.066MetAsn: 1.066 ± 0.172
0.935MetPro: 0.935 ± 0.149
0.591MetGln: 0.591 ± 0.107
0.574MetArg: 0.574 ± 0.09
1.525MetSer: 1.525 ± 0.215
2.018MetThr: 2.018 ± 0.32
0.968MetVal: 0.968 ± 0.13
0.23MetTrp: 0.23 ± 0.071
0.787MetTyr: 0.787 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.674AsnAla: 3.674 ± 0.298
0.476AsnCys: 0.476 ± 0.098
3.773AsnAsp: 3.773 ± 0.281
3.363AsnGlu: 3.363 ± 0.249
3.592AsnPhe: 3.592 ± 0.263
5.167AsnGly: 5.167 ± 0.347
0.886AsnHis: 0.886 ± 0.119
4.724AsnIle: 4.724 ± 0.339
3.313AsnLys: 3.313 ± 0.219
5.167AsnLeu: 5.167 ± 0.294
0.869AsnMet: 0.869 ± 0.169
3.74AsnAsn: 3.74 ± 0.351
3.412AsnPro: 3.412 ± 0.297
2.346AsnGln: 2.346 ± 0.174
2.329AsnArg: 2.329 ± 0.202
4.544AsnSer: 4.544 ± 0.421
4.56AsnThr: 4.56 ± 0.434
4.002AsnVal: 4.002 ± 0.337
0.623AsnTrp: 0.623 ± 0.099
2.789AsnTyr: 2.789 ± 0.228
0.0AsnXaa: 0.0 ± 0.0
Pro
2.592ProAla: 2.592 ± 0.3
0.197ProCys: 0.197 ± 0.062
2.903ProAsp: 2.903 ± 0.226
2.985ProGlu: 2.985 ± 0.297
1.542ProPhe: 1.542 ± 0.18
3.363ProGly: 3.363 ± 0.258
0.476ProHis: 0.476 ± 0.098
2.378ProIle: 2.378 ± 0.256
2.329ProLys: 2.329 ± 0.221
2.674ProLeu: 2.674 ± 0.221
0.64ProMet: 0.64 ± 0.141
2.493ProAsn: 2.493 ± 0.277
1.575ProPro: 1.575 ± 0.173
1.378ProGln: 1.378 ± 0.17
1.345ProArg: 1.345 ± 0.117
3.297ProSer: 3.297 ± 0.241
2.592ProThr: 2.592 ± 0.178
2.477ProVal: 2.477 ± 0.261
0.525ProTrp: 0.525 ± 0.099
1.69ProTyr: 1.69 ± 0.151
0.0ProXaa: 0.0 ± 0.0
Gln
1.657GlnAla: 1.657 ± 0.169
0.23GlnCys: 0.23 ± 0.064
1.919GlnAsp: 1.919 ± 0.176
2.641GlnGlu: 2.641 ± 0.234
1.608GlnPhe: 1.608 ± 0.185
2.1GlnGly: 2.1 ± 0.195
0.459GlnHis: 0.459 ± 0.09
2.821GlnIle: 2.821 ± 0.216
2.674GlnLys: 2.674 ± 0.284
2.936GlnLeu: 2.936 ± 0.262
0.968GlnMet: 0.968 ± 0.158
2.001GlnAsn: 2.001 ± 0.171
1.411GlnPro: 1.411 ± 0.13
1.591GlnGln: 1.591 ± 0.148
1.69GlnArg: 1.69 ± 0.208
2.69GlnSer: 2.69 ± 0.219
1.952GlnThr: 1.952 ± 0.167
2.1GlnVal: 2.1 ± 0.171
0.328GlnTrp: 0.328 ± 0.08
1.673GlnTyr: 1.673 ± 0.199
0.0GlnXaa: 0.0 ± 0.0
Arg
2.264ArgAla: 2.264 ± 0.197
0.295ArgCys: 0.295 ± 0.072
2.608ArgAsp: 2.608 ± 0.185
2.477ArgGlu: 2.477 ± 0.209
2.132ArgPhe: 2.132 ± 0.165
3.117ArgGly: 3.117 ± 0.264
0.344ArgHis: 0.344 ± 0.081
2.772ArgIle: 2.772 ± 0.225
2.805ArgLys: 2.805 ± 0.308
3.527ArgLeu: 3.527 ± 0.233
0.869ArgMet: 0.869 ± 0.146
1.985ArgAsn: 1.985 ± 0.176
1.427ArgPro: 1.427 ± 0.129
1.493ArgGln: 1.493 ± 0.15
2.182ArgArg: 2.182 ± 0.253
2.838ArgSer: 2.838 ± 0.218
2.362ArgThr: 2.362 ± 0.196
2.969ArgVal: 2.969 ± 0.264
0.623ArgTrp: 0.623 ± 0.115
2.313ArgTyr: 2.313 ± 0.212
0.0ArgXaa: 0.0 ± 0.0
Ser
4.806SerAla: 4.806 ± 0.313
0.623SerCys: 0.623 ± 0.098
4.576SerAsp: 4.576 ± 0.212
3.51SerGlu: 3.51 ± 0.254
3.363SerPhe: 3.363 ± 0.209
7.759SerGly: 7.759 ± 0.491
0.951SerHis: 0.951 ± 0.145
5.643SerIle: 5.643 ± 0.369
3.576SerLys: 3.576 ± 0.291
5.659SerLeu: 5.659 ± 0.357
1.181SerMet: 1.181 ± 0.15
5.561SerAsn: 5.561 ± 0.479
2.756SerPro: 2.756 ± 0.25
2.28SerGln: 2.28 ± 0.184
2.641SerArg: 2.641 ± 0.216
6.643SerSer: 6.643 ± 0.451
5.347SerThr: 5.347 ± 0.37
5.757SerVal: 5.757 ± 0.397
0.853SerTrp: 0.853 ± 0.105
3.133SerTyr: 3.133 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
4.576ThrAla: 4.576 ± 0.414
0.508ThrCys: 0.508 ± 0.103
4.396ThrAsp: 4.396 ± 0.315
3.806ThrGlu: 3.806 ± 0.249
3.691ThrPhe: 3.691 ± 0.294
6.332ThrGly: 6.332 ± 0.589
0.755ThrHis: 0.755 ± 0.129
6.807ThrIle: 6.807 ± 0.504
3.559ThrLys: 3.559 ± 0.284
5.626ThrLeu: 5.626 ± 0.49
0.984ThrMet: 0.984 ± 0.153
4.445ThrAsn: 4.445 ± 0.402
3.231ThrPro: 3.231 ± 0.226
2.067ThrGln: 2.067 ± 0.148
2.346ThrArg: 2.346 ± 0.207
5.61ThrSer: 5.61 ± 0.456
5.479ThrThr: 5.479 ± 0.506
2.657ThrVal: 2.657 ± 0.261
0.853ThrTrp: 0.853 ± 0.117
2.821ThrTyr: 2.821 ± 0.218
0.0ThrXaa: 0.0 ± 0.0
Val
4.347ValAla: 4.347 ± 0.317
0.591ValCys: 0.591 ± 0.115
4.757ValAsp: 4.757 ± 0.27
4.15ValGlu: 4.15 ± 0.267
3.084ValPhe: 3.084 ± 0.251
4.593ValGly: 4.593 ± 0.415
0.673ValHis: 0.673 ± 0.129
3.904ValIle: 3.904 ± 0.266
3.51ValLys: 3.51 ± 0.281
4.347ValLeu: 4.347 ± 0.235
1.066ValMet: 1.066 ± 0.154
3.986ValAsn: 3.986 ± 0.248
2.46ValPro: 2.46 ± 0.208
1.886ValGln: 1.886 ± 0.19
3.051ValArg: 3.051 ± 0.193
5.134ValSer: 5.134 ± 0.38
5.019ValThr: 5.019 ± 0.5
4.576ValVal: 4.576 ± 0.306
0.476ValTrp: 0.476 ± 0.071
2.346ValTyr: 2.346 ± 0.197
0.0ValXaa: 0.0 ± 0.0
Trp
0.508TrpAla: 0.508 ± 0.089
0.131TrpCys: 0.131 ± 0.043
0.935TrpAsp: 0.935 ± 0.151
0.738TrpGlu: 0.738 ± 0.134
0.459TrpPhe: 0.459 ± 0.112
0.64TrpGly: 0.64 ± 0.121
0.361TrpHis: 0.361 ± 0.083
0.82TrpIle: 0.82 ± 0.12
0.64TrpLys: 0.64 ± 0.116
0.541TrpLeu: 0.541 ± 0.105
0.279TrpMet: 0.279 ± 0.084
0.82TrpAsn: 0.82 ± 0.146
0.148TrpPro: 0.148 ± 0.042
0.443TrpGln: 0.443 ± 0.097
0.591TrpArg: 0.591 ± 0.118
0.705TrpSer: 0.705 ± 0.11
0.787TrpThr: 0.787 ± 0.12
0.591TrpVal: 0.591 ± 0.106
0.23TrpTrp: 0.23 ± 0.086
0.574TrpTyr: 0.574 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.542TyrAla: 2.542 ± 0.218
0.328TyrCys: 0.328 ± 0.093
3.33TyrAsp: 3.33 ± 0.268
2.641TyrGlu: 2.641 ± 0.229
2.182TyrPhe: 2.182 ± 0.174
2.444TyrGly: 2.444 ± 0.214
0.574TyrHis: 0.574 ± 0.107
2.985TyrIle: 2.985 ± 0.231
2.116TyrLys: 2.116 ± 0.219
3.281TyrLeu: 3.281 ± 0.275
0.738TyrMet: 0.738 ± 0.12
3.018TyrAsn: 3.018 ± 0.199
1.936TyrPro: 1.936 ± 0.171
1.821TyrGln: 1.821 ± 0.158
2.001TyrArg: 2.001 ± 0.19
3.002TyrSer: 3.002 ± 0.228
3.067TyrThr: 3.067 ± 0.25
3.166TyrVal: 3.166 ± 0.296
0.476TyrTrp: 0.476 ± 0.081
2.346TyrTyr: 2.346 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 243 proteins (60965 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski