Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales;

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4CL42|K4CL42_SOLLC Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1
MM1 pKa = 7.64PSSDD5 pKa = 4.05YY6 pKa = 10.22FHH8 pKa = 7.13CRR10 pKa = 11.84CGTRR14 pKa = 11.84VALVQDD20 pKa = 3.64YY21 pKa = 11.26VEE23 pKa = 4.7IDD25 pKa = 3.52EE26 pKa = 4.88EE27 pKa = 4.62LTFVLQGIFTDD38 pKa = 3.81MFNVVVPEE46 pKa = 3.88NDD48 pKa = 3.04EE49 pKa = 4.81SYY51 pKa = 10.81HH52 pKa = 5.96QVLNGNTVVDD62 pKa = 4.21TLCVNCRR69 pKa = 11.84DD70 pKa = 3.49RR71 pKa = 11.84LGWKK75 pKa = 9.85FIAVAQGSPYY85 pKa = 10.45EE86 pKa = 4.21AGQFLMMLDD95 pKa = 3.35KK96 pKa = 11.33LNYY99 pKa = 9.89TNGQLLDD106 pKa = 4.16PYY108 pKa = 10.59EE109 pKa = 4.63LGGGPNEE116 pKa = 5.01DD117 pKa = 4.1NVDD120 pKa = 3.63QAGGANNEE128 pKa = 4.13EE129 pKa = 4.35NADD132 pKa = 3.93NQDD135 pKa = 3.26GGANNQDD142 pKa = 2.87GVANNADD149 pKa = 3.87NQDD152 pKa = 3.49GGANNQDD159 pKa = 3.1GGANNADD166 pKa = 3.77NQDD169 pKa = 3.35GGGDD173 pKa = 3.46QLVVSNGHH181 pKa = 5.88SDD183 pKa = 3.56RR184 pKa = 11.84NPNII188 pKa = 3.57

Molecular weight: 20.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4CTR1|K4CTR1_SOLLC Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34652

6

34658

12261248

5

4819

353.8

39.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.27 ± 0.014

1.915 ± 0.007

5.261 ± 0.009

6.307 ± 0.014

4.356 ± 0.009

6.294 ± 0.014

2.42 ± 0.006

5.76 ± 0.01

6.353 ± 0.013

9.658 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.005

4.74 ± 0.01

4.713 ± 0.014

3.657 ± 0.009

5.077 ± 0.01

8.891 ± 0.016

5.025 ± 0.008

6.512 ± 0.01

1.282 ± 0.005

2.975 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski