Bosea sp. WAO

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4978 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A124GB96|A0A124GB96_9BRAD Uncharacterized protein OS=Bosea sp. WAO OX=406341 GN=DK26_22450 PE=4 SV=1
DDD2 pKa = 4.17ATVTEEE8 pKa = 4.47GFGATTGTVGSALSGAYYY26 pKa = 9.39ALTLNADDD34 pKa = 3.56SYYY37 pKa = 10.72YYY39 pKa = 11.59LDDD42 pKa = 3.78SDDD45 pKa = 3.52RR46 pKa = 11.84VNALRR51 pKa = 11.84DDD53 pKa = 3.75EEE55 pKa = 4.44LTEEE59 pKa = 4.04FTYYY63 pKa = 10.59ITDDD67 pKa = 3.4DDD69 pKa = 3.99DDD71 pKa = 4.06EE72 pKa = 4.5TATLTITITGRR83 pKa = 11.84TDDD86 pKa = 2.66TTQIVPGDDD95 pKa = 3.88NGAAHHH101 pKa = 7.04EEE103 pKa = 4.11SVSEEE108 pKa = 4.41GLGDDD113 pKa = 3.95GDDD116 pKa = 4.07SEEE119 pKa = 4.46TTGTVTVTAPDDD131 pKa = 3.63LSTVTVGGRR140 pKa = 11.84VITLAEEE147 pKa = 4.11QALGTSPITVTTPKKK162 pKa = 10.7ILTITGFTPGTTVGGVPIEEE182 pKa = 4.53DDD184 pKa = 3.01SYYY187 pKa = 11.0YYY189 pKa = 11.24EE190 pKa = 3.99TTLQDDD196 pKa = 3.5HH197 pKa = 6.82GGAVDDD203 pKa = 5.98DD204 pKa = 4.33FALEEE209 pKa = 4.32GDDD212 pKa = 4.48GGGSATGTLTIAIADDD228 pKa = 4.22VPQAQDDD235 pKa = 3.25DD236 pKa = 4.2ATIAEEE242 pKa = 4.86DD243 pKa = 3.93DD244 pKa = 4.34SDDD247 pKa = 3.77VDDD250 pKa = 3.23NVFSGVGPGDDD261 pKa = 3.77ADDD264 pKa = 3.94AGNDDD269 pKa = 3.71PATGGPVTGVGFGTTSGTVGSGLSGAYYY297 pKa = 7.67TLTLNADDD305 pKa = 3.47SYYY308 pKa = 10.78YYY310 pKa = 11.54LDDD313 pKa = 3.93GNAAVNGLRR322 pKa = 11.84DDD324 pKa = 3.84QTLTEEE330 pKa = 4.1FSYYY334 pKa = 10.54ISDDD338 pKa = 3.7DDD340 pKa = 3.58STSEEE345 pKa = 4.12TLTITI

Molecular weight: 34.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101K3Z8|A0A101K3Z8_9BRAD Gluconolactonase OS=Bosea sp. WAO OX=406341 GN=DK26_03855 PE=4 SV=1
MM1 pKa = 7.43GFSLLKK7 pKa = 10.07TRR9 pKa = 11.84FAQEE13 pKa = 3.34ALGRR17 pKa = 11.84SLAWYY22 pKa = 9.22LRR24 pKa = 11.84LVRR27 pKa = 11.84RR28 pKa = 11.84TNRR31 pKa = 11.84FVVEE35 pKa = 3.74PADD38 pKa = 3.02IYY40 pKa = 11.34EE41 pKa = 4.31RR42 pKa = 11.84VRR44 pKa = 11.84PDD46 pKa = 3.34LPLIIAMWHH55 pKa = 5.87GQHH58 pKa = 6.93IMIPFARR65 pKa = 11.84PDD67 pKa = 3.3WMPACSLVSRR77 pKa = 11.84HH78 pKa = 5.92GDD80 pKa = 3.04GGFNAVALRR89 pKa = 11.84EE90 pKa = 4.09LGIGAIRR97 pKa = 11.84GSGAMGKK104 pKa = 9.51KK105 pKa = 9.61VRR107 pKa = 11.84EE108 pKa = 4.19KK109 pKa = 10.87GGAPAFLAMMRR120 pKa = 11.84RR121 pKa = 11.84LAAGEE126 pKa = 4.24TMVLTADD133 pKa = 3.03IPKK136 pKa = 9.98RR137 pKa = 11.84ARR139 pKa = 11.84VCGAGIVALARR150 pKa = 11.84ASGRR154 pKa = 11.84PIHH157 pKa = 6.79PIAVVTSRR165 pKa = 11.84RR166 pKa = 11.84IDD168 pKa = 3.49FNSWDD173 pKa = 3.8RR174 pKa = 11.84ASIGLPFGRR183 pKa = 11.84GAIVVGEE190 pKa = 4.31AVTVARR196 pKa = 11.84DD197 pKa = 3.26ADD199 pKa = 3.88EE200 pKa = 4.6ATCEE204 pKa = 3.88AARR207 pKa = 11.84LAVQAGLDD215 pKa = 3.67AAHH218 pKa = 6.5EE219 pKa = 3.98RR220 pKa = 11.84AYY222 pKa = 10.92ALIGARR228 pKa = 11.84DD229 pKa = 3.63PGAGLRR235 pKa = 11.84EE236 pKa = 3.63AAAAKK241 pKa = 10.2DD242 pKa = 3.53AATAAAKK249 pKa = 10.17AATPP253 pKa = 3.8

Molecular weight: 26.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4978

0

4978

1552009

41

6267

311.8

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.507 ± 0.05

0.811 ± 0.012

5.174 ± 0.03

5.705 ± 0.038

3.706 ± 0.026

9.017 ± 0.051

1.878 ± 0.016

5.073 ± 0.024

3.227 ± 0.033

10.623 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.018

2.358 ± 0.022

5.318 ± 0.033

3.032 ± 0.017

7.212 ± 0.037

5.217 ± 0.037

5.036 ± 0.039

7.406 ± 0.024

1.299 ± 0.014

2.073 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski