Amino acid dipepetide frequency for Bosea sp. WAO

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.547AlaAla: 19.547 ± 0.183
1.126AlaCys: 1.126 ± 0.031
6.303AlaAsp: 6.303 ± 0.064
8.363AlaGlu: 8.363 ± 0.09
4.917AlaPhe: 4.917 ± 0.064
12.537AlaGly: 12.537 ± 0.139
2.177AlaHis: 2.177 ± 0.04
6.945AlaIle: 6.945 ± 0.083
4.395AlaLys: 4.395 ± 0.068
15.526AlaLeu: 15.526 ± 0.126
3.643AlaMet: 3.643 ± 0.051
2.934AlaAsn: 2.934 ± 0.048
6.14AlaPro: 6.14 ± 0.084
4.375AlaGln: 4.375 ± 0.059
9.666AlaArg: 9.666 ± 0.1
6.831AlaSer: 6.831 ± 0.079
6.379AlaThr: 6.379 ± 0.079
9.128AlaVal: 9.128 ± 0.075
1.517AlaTrp: 1.517 ± 0.031
2.623AlaTyr: 2.623 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.991CysAla: 0.991 ± 0.028
0.095CysCys: 0.095 ± 0.009
0.47CysAsp: 0.47 ± 0.017
0.472CysGlu: 0.472 ± 0.02
0.346CysPhe: 0.346 ± 0.017
0.94CysGly: 0.94 ± 0.026
0.232CysHis: 0.232 ± 0.013
0.381CysIle: 0.381 ± 0.014
0.184CysLys: 0.184 ± 0.011
0.798CysLeu: 0.798 ± 0.023
0.13CysMet: 0.13 ± 0.01
0.186CysAsn: 0.186 ± 0.01
0.427CysPro: 0.427 ± 0.016
0.197CysGln: 0.197 ± 0.011
0.616CysArg: 0.616 ± 0.019
0.428CysSer: 0.428 ± 0.017
0.361CysThr: 0.361 ± 0.014
0.573CysVal: 0.573 ± 0.02
0.106CysTrp: 0.106 ± 0.009
0.172CysTyr: 0.172 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.588AspAla: 6.588 ± 0.071
0.46AspCys: 0.46 ± 0.019
2.573AspAsp: 2.573 ± 0.053
3.411AspGlu: 3.411 ± 0.054
2.031AspPhe: 2.031 ± 0.041
5.106AspGly: 5.106 ± 0.07
1.06AspHis: 1.06 ± 0.027
2.738AspIle: 2.738 ± 0.048
1.673AspLys: 1.673 ± 0.043
5.586AspLeu: 5.586 ± 0.062
1.095AspMet: 1.095 ± 0.029
0.992AspAsn: 0.992 ± 0.029
3.316AspPro: 3.316 ± 0.05
1.47AspGln: 1.47 ± 0.027
3.833AspArg: 3.833 ± 0.052
1.906AspSer: 1.906 ± 0.038
2.064AspThr: 2.064 ± 0.039
3.651AspVal: 3.651 ± 0.057
0.88AspTrp: 0.88 ± 0.025
1.311AspTyr: 1.311 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
8.672GluAla: 8.672 ± 0.09
0.323GluCys: 0.323 ± 0.016
2.41GluAsp: 2.41 ± 0.039
3.052GluGlu: 3.052 ± 0.059
1.702GluPhe: 1.702 ± 0.039
4.338GluGly: 4.338 ± 0.06
1.098GluHis: 1.098 ± 0.026
3.624GluIle: 3.624 ± 0.051
2.127GluLys: 2.127 ± 0.046
5.711GluLeu: 5.711 ± 0.076
1.405GluMet: 1.405 ± 0.03
1.341GluAsn: 1.341 ± 0.032
3.022GluPro: 3.022 ± 0.051
2.018GluGln: 2.018 ± 0.035
5.408GluArg: 5.408 ± 0.071
2.25GluSer: 2.25 ± 0.037
3.3GluThr: 3.3 ± 0.043
3.811GluVal: 3.811 ± 0.055
0.631GluTrp: 0.631 ± 0.021
0.81GluTyr: 0.81 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
5.017PheAla: 5.017 ± 0.055
0.403PheCys: 0.403 ± 0.016
2.449PheAsp: 2.449 ± 0.038
2.062PheGlu: 2.062 ± 0.038
1.402PhePhe: 1.402 ± 0.033
3.83PheGly: 3.83 ± 0.059
0.707PheHis: 0.707 ± 0.023
1.635PheIle: 1.635 ± 0.034
1.037PheLys: 1.037 ± 0.028
3.417PheLeu: 3.417 ± 0.055
0.822PheMet: 0.822 ± 0.026
0.97PheAsn: 0.97 ± 0.027
1.654PhePro: 1.654 ± 0.034
0.991PheGln: 0.991 ± 0.026
2.285PheArg: 2.285 ± 0.038
2.173PheSer: 2.173 ± 0.036
1.87PheThr: 1.87 ± 0.037
2.957PheVal: 2.957 ± 0.049
0.558PheTrp: 0.558 ± 0.023
0.822PheTyr: 0.822 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.426GlyAla: 10.426 ± 0.116
0.867GlyCys: 0.867 ± 0.025
4.202GlyAsp: 4.202 ± 0.062
5.32GlyGlu: 5.32 ± 0.063
3.869GlyPhe: 3.869 ± 0.049
8.125GlyGly: 8.125 ± 0.187
1.934GlyHis: 1.934 ± 0.037
4.717GlyIle: 4.717 ± 0.069
3.499GlyLys: 3.499 ± 0.057
10.086GlyLeu: 10.086 ± 0.099
2.297GlyMet: 2.297 ± 0.038
2.104GlyAsn: 2.104 ± 0.053
3.814GlyPro: 3.814 ± 0.051
2.941GlyGln: 2.941 ± 0.047
6.613GlyArg: 6.613 ± 0.069
4.839GlySer: 4.839 ± 0.103
4.548GlyThr: 4.548 ± 0.083
6.283GlyVal: 6.283 ± 0.078
1.434GlyTrp: 1.434 ± 0.036
2.256GlyTyr: 2.256 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.322HisAla: 2.322 ± 0.04
0.195HisCys: 0.195 ± 0.01
1.16HisAsp: 1.16 ± 0.031
1.005HisGlu: 1.005 ± 0.028
0.764HisPhe: 0.764 ± 0.024
1.973HisGly: 1.973 ± 0.039
0.483HisHis: 0.483 ± 0.021
0.816HisIle: 0.816 ± 0.022
0.455HisLys: 0.455 ± 0.018
1.992HisLeu: 1.992 ± 0.035
0.401HisMet: 0.401 ± 0.015
0.393HisAsn: 0.393 ± 0.017
1.251HisPro: 1.251 ± 0.03
0.504HisGln: 0.504 ± 0.017
1.305HisArg: 1.305 ± 0.034
0.816HisSer: 0.816 ± 0.022
0.682HisThr: 0.682 ± 0.022
1.467HisVal: 1.467 ± 0.036
0.309HisTrp: 0.309 ± 0.013
0.483HisTyr: 0.483 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
7.973IleAla: 7.973 ± 0.07
0.505IleCys: 0.505 ± 0.018
3.193IleAsp: 3.193 ± 0.046
3.441IleGlu: 3.441 ± 0.054
1.617IlePhe: 1.617 ± 0.037
5.369IleGly: 5.369 ± 0.064
0.827IleHis: 0.827 ± 0.023
1.995IleIle: 1.995 ± 0.044
1.399IleLys: 1.399 ± 0.038
4.45IleLeu: 4.45 ± 0.056
0.954IleMet: 0.954 ± 0.027
1.218IleAsn: 1.218 ± 0.032
2.249IlePro: 2.249 ± 0.04
1.108IleGln: 1.108 ± 0.024
3.122IleArg: 3.122 ± 0.047
2.616IleSer: 2.616 ± 0.045
2.532IleThr: 2.532 ± 0.053
4.555IleVal: 4.555 ± 0.057
0.613IleTrp: 0.613 ± 0.019
0.999IleTyr: 0.999 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.665LysAla: 4.665 ± 0.066
0.135LysCys: 0.135 ± 0.009
1.589LysAsp: 1.589 ± 0.037
1.546LysGlu: 1.546 ± 0.036
0.829LysPhe: 0.829 ± 0.024
2.729LysGly: 2.729 ± 0.046
0.552LysHis: 0.552 ± 0.018
1.58LysIle: 1.58 ± 0.034
1.111LysLys: 1.111 ± 0.035
3.743LysLeu: 3.743 ± 0.049
0.627LysMet: 0.627 ± 0.022
0.796LysAsn: 0.796 ± 0.025
2.282LysPro: 2.282 ± 0.049
1.007LysGln: 1.007 ± 0.031
2.384LysArg: 2.384 ± 0.043
1.612LysSer: 1.612 ± 0.034
1.741LysThr: 1.741 ± 0.039
2.526LysVal: 2.526 ± 0.046
0.311LysTrp: 0.311 ± 0.014
0.501LysTyr: 0.501 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
16.111LeuAla: 16.111 ± 0.13
0.869LeuCys: 0.869 ± 0.025
6.09LeuAsp: 6.09 ± 0.07
5.035LeuGlu: 5.035 ± 0.067
3.668LeuPhe: 3.668 ± 0.063
9.328LeuGly: 9.328 ± 0.095
1.756LeuHis: 1.756 ± 0.031
5.269LeuIle: 5.269 ± 0.072
3.779LeuLys: 3.779 ± 0.06
10.231LeuLeu: 10.231 ± 0.13
2.298LeuMet: 2.298 ± 0.044
2.424LeuAsn: 2.424 ± 0.04
5.986LeuPro: 5.986 ± 0.069
2.618LeuGln: 2.618 ± 0.043
7.235LeuArg: 7.235 ± 0.072
6.497LeuSer: 6.497 ± 0.08
5.595LeuThr: 5.595 ± 0.069
8.212LeuVal: 8.212 ± 0.093
1.245LeuTrp: 1.245 ± 0.027
1.987LeuTyr: 1.987 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.075MetAla: 3.075 ± 0.052
0.131MetCys: 0.131 ± 0.008
0.943MetAsp: 0.943 ± 0.026
0.967MetGlu: 0.967 ± 0.027
0.626MetPhe: 0.626 ± 0.019
1.713MetGly: 1.713 ± 0.029
0.374MetHis: 0.374 ± 0.016
1.221MetIle: 1.221 ± 0.029
0.882MetLys: 0.882 ± 0.025
2.664MetLeu: 2.664 ± 0.05
0.555MetMet: 0.555 ± 0.021
0.685MetAsn: 0.685 ± 0.02
1.497MetPro: 1.497 ± 0.034
0.758MetGln: 0.758 ± 0.023
1.815MetArg: 1.815 ± 0.034
1.533MetSer: 1.533 ± 0.035
1.738MetThr: 1.738 ± 0.034
1.676MetVal: 1.676 ± 0.039
0.176MetTrp: 0.176 ± 0.01
0.248MetTyr: 0.248 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.055AsnAla: 3.055 ± 0.052
0.213AsnCys: 0.213 ± 0.012
1.19AsnAsp: 1.19 ± 0.038
1.141AsnGlu: 1.141 ± 0.029
0.878AsnPhe: 0.878 ± 0.024
2.253AsnGly: 2.253 ± 0.053
0.432AsnHis: 0.432 ± 0.016
1.139AsnIle: 1.139 ± 0.031
0.648AsnLys: 0.648 ± 0.022
2.505AsnLeu: 2.505 ± 0.037
0.508AsnMet: 0.508 ± 0.019
0.631AsnAsn: 0.631 ± 0.024
1.717AsnPro: 1.717 ± 0.034
0.674AsnGln: 0.674 ± 0.022
1.666AsnArg: 1.666 ± 0.028
1.107AsnSer: 1.107 ± 0.03
1.053AsnThr: 1.053 ± 0.028
1.751AsnVal: 1.751 ± 0.038
0.408AsnTrp: 0.408 ± 0.019
0.611AsnTyr: 0.611 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
7.324ProAla: 7.324 ± 0.1
0.296ProCys: 0.296 ± 0.015
3.255ProAsp: 3.255 ± 0.042
3.788ProGlu: 3.788 ± 0.05
2.029ProPhe: 2.029 ± 0.042
4.963ProGly: 4.963 ± 0.06
1.027ProHis: 1.027 ± 0.026
2.314ProIle: 2.314 ± 0.039
1.72ProLys: 1.72 ± 0.038
5.035ProLeu: 5.035 ± 0.066
1.2ProMet: 1.2 ± 0.028
1.3ProAsn: 1.3 ± 0.029
2.697ProPro: 2.697 ± 0.057
1.793ProGln: 1.793 ± 0.031
3.09ProArg: 3.09 ± 0.054
2.782ProSer: 2.782 ± 0.052
2.45ProThr: 2.45 ± 0.037
4.29ProVal: 4.29 ± 0.052
0.689ProTrp: 0.689 ± 0.023
1.139ProTyr: 1.139 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
4.532GlnAla: 4.532 ± 0.055
0.176GlnCys: 0.176 ± 0.01
1.359GlnAsp: 1.359 ± 0.029
1.438GlnGlu: 1.438 ± 0.032
0.992GlnPhe: 0.992 ± 0.028
2.493GlnGly: 2.493 ± 0.043
0.588GlnHis: 0.588 ± 0.02
1.764GlnIle: 1.764 ± 0.03
0.97GlnLys: 0.97 ± 0.029
2.778GlnLeu: 2.778 ± 0.042
0.76GlnMet: 0.76 ± 0.021
0.812GlnAsn: 0.812 ± 0.023
1.736GlnPro: 1.736 ± 0.043
1.202GlnGln: 1.202 ± 0.033
2.58GlnArg: 2.58 ± 0.046
1.624GlnSer: 1.624 ± 0.036
1.535GlnThr: 1.535 ± 0.031
2.162GlnVal: 2.162 ± 0.037
0.329GlnTrp: 0.329 ± 0.016
0.494GlnTyr: 0.494 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
8.368ArgAla: 8.368 ± 0.084
0.524ArgCys: 0.524 ± 0.019
3.959ArgAsp: 3.959 ± 0.05
4.534ArgGlu: 4.534 ± 0.056
3.101ArgPhe: 3.101 ± 0.049
5.042ArgGly: 5.042 ± 0.057
1.66ArgHis: 1.66 ± 0.04
4.013ArgIle: 4.013 ± 0.05
2.224ArgLys: 2.224 ± 0.039
8.514ArgLeu: 8.514 ± 0.091
1.793ArgMet: 1.793 ± 0.036
1.744ArgAsn: 1.744 ± 0.031
3.802ArgPro: 3.802 ± 0.05
2.673ArgGln: 2.673 ± 0.048
5.95ArgArg: 5.95 ± 0.081
3.74ArgSer: 3.74 ± 0.054
3.175ArgThr: 3.175 ± 0.045
4.595ArgVal: 4.595 ± 0.058
1.014ArgTrp: 1.014 ± 0.026
1.694ArgTyr: 1.694 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.389SerAla: 6.389 ± 0.096
0.483SerCys: 0.483 ± 0.018
2.563SerAsp: 2.563 ± 0.047
2.731SerGlu: 2.731 ± 0.046
2.403SerPhe: 2.403 ± 0.044
5.625SerGly: 5.625 ± 0.091
0.997SerHis: 0.997 ± 0.027
2.559SerIle: 2.559 ± 0.045
1.461SerLys: 1.461 ± 0.039
5.666SerLeu: 5.666 ± 0.073
1.131SerMet: 1.131 ± 0.025
1.174SerAsn: 1.174 ± 0.031
2.751SerPro: 2.751 ± 0.039
1.559SerGln: 1.559 ± 0.033
3.522SerArg: 3.522 ± 0.049
2.729SerSer: 2.729 ± 0.061
2.48SerThr: 2.48 ± 0.049
3.816SerVal: 3.816 ± 0.057
0.8SerTrp: 0.8 ± 0.025
1.325SerTyr: 1.325 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
6.157ThrAla: 6.157 ± 0.097
0.374ThrCys: 0.374 ± 0.017
2.279ThrAsp: 2.279 ± 0.04
2.376ThrGlu: 2.376 ± 0.044
1.776ThrPhe: 1.776 ± 0.037
5.044ThrGly: 5.044 ± 0.078
0.9ThrHis: 0.9 ± 0.024
2.847ThrIle: 2.847 ± 0.048
1.382ThrLys: 1.382 ± 0.036
5.945ThrLeu: 5.945 ± 0.067
1.12ThrMet: 1.12 ± 0.029
1.147ThrAsn: 1.147 ± 0.035
3.16ThrPro: 3.16 ± 0.047
1.389ThrGln: 1.389 ± 0.031
3.291ThrArg: 3.291 ± 0.045
2.586ThrSer: 2.586 ± 0.052
2.756ThrThr: 2.756 ± 0.059
4.191ThrVal: 4.191 ± 0.054
0.585ThrTrp: 0.585 ± 0.022
1.055ThrTyr: 1.055 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.061ValAla: 10.061 ± 0.09
0.584ValCys: 0.584 ± 0.019
3.87ValAsp: 3.87 ± 0.051
4.687ValGlu: 4.687 ± 0.063
2.74ValPhe: 2.74 ± 0.052
5.759ValGly: 5.759 ± 0.063
1.245ValHis: 1.245 ± 0.029
3.847ValIle: 3.847 ± 0.057
2.334ValLys: 2.334 ± 0.045
7.639ValLeu: 7.639 ± 0.084
1.876ValMet: 1.876 ± 0.039
1.833ValAsn: 1.833 ± 0.04
3.839ValPro: 3.839 ± 0.048
1.885ValGln: 1.885 ± 0.039
4.829ValArg: 4.829 ± 0.055
4.249ValSer: 4.249 ± 0.061
4.387ValThr: 4.387 ± 0.064
6.177ValVal: 6.177 ± 0.068
0.813ValTrp: 0.813 ± 0.027
1.403ValTyr: 1.403 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.247TrpAla: 1.247 ± 0.028
0.137TrpCys: 0.137 ± 0.009
0.578TrpAsp: 0.578 ± 0.023
0.541TrpGlu: 0.541 ± 0.019
0.548TrpPhe: 0.548 ± 0.021
0.905TrpGly: 0.905 ± 0.025
0.325TrpHis: 0.325 ± 0.015
0.59TrpIle: 0.59 ± 0.02
0.429TrpLys: 0.429 ± 0.017
1.738TrpLeu: 1.738 ± 0.04
0.305TrpMet: 0.305 ± 0.013
0.414TrpAsn: 0.414 ± 0.017
0.741TrpPro: 0.741 ± 0.024
0.526TrpGln: 0.526 ± 0.017
1.184TrpArg: 1.184 ± 0.029
0.829TrpSer: 0.829 ± 0.025
0.742TrpThr: 0.742 ± 0.025
0.715TrpVal: 0.715 ± 0.023
0.227TrpTrp: 0.227 ± 0.012
0.273TrpTyr: 0.273 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.553TyrAla: 2.553 ± 0.044
0.213TyrCys: 0.213 ± 0.012
1.309TyrAsp: 1.309 ± 0.029
1.14TyrGlu: 1.14 ± 0.027
0.825TyrPhe: 0.825 ± 0.026
2.105TyrGly: 2.105 ± 0.039
0.401TyrHis: 0.401 ± 0.013
0.741TyrIle: 0.741 ± 0.021
0.556TyrLys: 0.556 ± 0.021
2.207TyrLeu: 2.207 ± 0.044
0.355TyrMet: 0.355 ± 0.017
0.482TyrAsn: 0.482 ± 0.019
1.101TyrPro: 1.101 ± 0.028
0.633TyrGln: 0.633 ± 0.02
1.727TyrArg: 1.727 ± 0.033
1.017TyrSer: 1.017 ± 0.029
0.974TyrThr: 0.974 ± 0.027
1.521TyrVal: 1.521 ± 0.033
0.347TyrTrp: 0.347 ± 0.016
0.523TyrTyr: 0.523 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4978 proteins (1552009 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski