Desulfuromonas sp. DDH964
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3483 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143B473|A0A143B473_9DELT UbiD family decarboxylase OS=Desulfuromonas sp. DDH964 OX=1823759 GN=DBW_0493 PE=4 SV=1
MM1 pKa = 6.86 IQRR4 pKa = 11.84 RR5 pKa = 11.84 LLKK8 pKa = 9.02 WLPVAMLAAFLLAGCSGDD26 pKa = 3.5 NSSQGTPAGKK36 pKa = 10.02 DD37 pKa = 2.97 AVAQKK42 pKa = 9.94 ATVTFKK48 pKa = 11.25 VQFPEE53 pKa = 4.25 SAVQKK58 pKa = 11.31 AMIDD62 pKa = 3.56 DD63 pKa = 3.9 RR64 pKa = 11.84 TVSVQVQWGDD74 pKa = 3.38 YY75 pKa = 9.34 YY76 pKa = 11.35 NYY78 pKa = 10.96 SFIDD82 pKa = 4.53 SITLTPDD89 pKa = 2.8 SSGMATATVTVPVGMLQFTAWANDD113 pKa = 3.52 ANGMEE118 pKa = 4.92 LEE120 pKa = 4.61 MISTAGDD127 pKa = 3.06 IVEE130 pKa = 4.76 GNNTVYY136 pKa = 9.91 LTFLGGDD143 pKa = 3.58 WQFVDD148 pKa = 5.47 ANDD151 pKa = 3.73 APMPLSVGTGASSRR165 pKa = 11.84 TLTGFSLGSAYY176 pKa = 10.81 SHH178 pKa = 6.19 GMYY181 pKa = 10.64 AKK183 pKa = 10.64 AKK185 pKa = 9.51 VDD187 pKa = 3.36 YY188 pKa = 7.62 TKK190 pKa = 11.09 PMGYY194 pKa = 10.34 GDD196 pKa = 5.61 YY197 pKa = 10.52 RR198 pKa = 11.84 LQWFDD203 pKa = 2.9 TAGAVGSQMAAWADD217 pKa = 3.45 NQLIGGVTNNTAFGSDD233 pKa = 3.8 MLNLTTPANSDD244 pKa = 3.31 NFSFWSFATAGDD256 pKa = 3.22 RR257 pKa = 11.84 VLFVVNSGPDD267 pKa = 2.84 SGTIKK272 pKa = 10.82 DD273 pKa = 3.82 GAGNDD278 pKa = 3.4 LTGAIDD284 pKa = 3.91 AVADD288 pKa = 3.87 AQILDD293 pKa = 3.72 GTHH296 pKa = 6.46 ISGHH300 pKa = 5.1 LLEE303 pKa = 4.54 MTFDD307 pKa = 3.83 SVTGIQTPTRR317 pKa = 11.84 TNIDD321 pKa = 3.55 CAPYY325 pKa = 8.0 WTYY328 pKa = 11.69 AGAARR333 pKa = 11.84 SAAIKK338 pKa = 10.29 AAFASSSQGPAKK350 pKa = 10.18 AAPGDD355 pKa = 4.07 SSTVTATLVAAYY367 pKa = 8.01 EE368 pKa = 4.25 EE369 pKa = 5.5 CNQQPYY375 pKa = 10.33 QIDD378 pKa = 3.64 GDD380 pKa = 3.96 GDD382 pKa = 3.42 QDD384 pKa = 3.33 FWYY387 pKa = 10.87 GDD389 pKa = 3.56 YY390 pKa = 10.66 LTFDD394 pKa = 4.0 FNSNNRR400 pKa = 11.84 YY401 pKa = 10.04 DD402 pKa = 3.95 PADD405 pKa = 3.79 GDD407 pKa = 4.29 TYY409 pKa = 11.48 QDD411 pKa = 3.67 TNNDD415 pKa = 3.18 GNFDD419 pKa = 3.69 FMIYY423 pKa = 10.29 NQVDD427 pKa = 3.33 GDD429 pKa = 4.07 GDD431 pKa = 4.69 GDD433 pKa = 4.14 MLWDD437 pKa = 3.69 YY438 pKa = 10.52 MIVDD442 pKa = 3.8 VNHH445 pKa = 6.58 NGRR448 pKa = 11.84 YY449 pKa = 9.21 DD450 pKa = 3.46 AADD453 pKa = 3.66 GDD455 pKa = 4.7 TYY457 pKa = 11.47 QDD459 pKa = 3.3 TDD461 pKa = 3.39 SDD463 pKa = 4.09 GKK465 pKa = 10.95 FDD467 pKa = 4.45 FVYY470 pKa = 10.55 TPGDD474 pKa = 3.7 LYY476 pKa = 11.21 AITEE480 pKa = 4.27 TFSNVVARR488 pKa = 11.84 EE489 pKa = 3.63 FRR491 pKa = 11.84 ARR493 pKa = 11.84 GSQIISQFTPSVLGTWGPAGYY514 pKa = 10.92 QMTGQGFAVSFLDD527 pKa = 3.36 ATHH530 pKa = 6.74 YY531 pKa = 10.71 IHH533 pKa = 7.66 VEE535 pKa = 3.85 DD536 pKa = 6.18 GIPDD540 pKa = 3.78 TDD542 pKa = 5.4 PITGNIIGGPGMEE555 pKa = 4.21 YY556 pKa = 9.23 GTYY559 pKa = 10.33 SVNATTGDD567 pKa = 3.61 VTFTASKK574 pKa = 9.55 DD575 pKa = 3.59 TTGDD579 pKa = 2.88 WGPAGTGTQVINFQFLDD596 pKa = 3.65 NNHH599 pKa = 5.81 FQITDD604 pKa = 3.74 PVDD607 pKa = 3.34 STTQTLGRR615 pKa = 11.84 VTSNWNSAVGSWKK628 pKa = 10.42 FGSEE632 pKa = 3.91 ALGMQVVTLFEE643 pKa = 4.54 GGNYY647 pKa = 9.51 LVASSGPPGTSNLDD661 pKa = 3.33 GMEE664 pKa = 4.12 SGTYY668 pKa = 9.67 SFQTISEE675 pKa = 4.69 SIDD678 pKa = 3.13 GTVTANITFNGMNSTIPDD696 pKa = 3.69 LNDD699 pKa = 3.31 TVIAAPGTSVTMPITMTNHH718 pKa = 5.7 GNTIEE723 pKa = 4.13 FDD725 pKa = 3.86 NNGTPVAFSRR735 pKa = 11.84 VQQ737 pKa = 2.84
Molecular weight: 78.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.783
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.503
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.834
Sillero 3.973
Patrickios 1.252
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A143BCB7|A0A143BCB7_9DELT Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase OS=Desulfuromonas sp. DDH964 OX=1823759 GN=phnM PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.94 RR4 pKa = 11.84 TYY6 pKa = 9.71 QPSRR10 pKa = 11.84 VSRR13 pKa = 11.84 KK14 pKa = 7.52 RR15 pKa = 11.84 THH17 pKa = 6.29 GFRR20 pKa = 11.84 KK21 pKa = 10.07 RR22 pKa = 11.84 MQSKK26 pKa = 10.01 NGRR29 pKa = 11.84 IVIKK33 pKa = 10.27 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.86 GRR40 pKa = 11.84 KK41 pKa = 8.29 NLVVTIPSKK50 pKa = 10.99
Molecular weight: 5.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3483
0
3483
1134986
29
3962
325.9
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.899 ± 0.06
1.208 ± 0.02
5.1 ± 0.027
6.425 ± 0.045
3.965 ± 0.03
8.41 ± 0.039
1.994 ± 0.021
5.107 ± 0.035
3.593 ± 0.039
11.844 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.022 ± 0.02
2.755 ± 0.031
5.053 ± 0.036
3.653 ± 0.024
7.091 ± 0.051
5.121 ± 0.031
4.989 ± 0.052
7.139 ± 0.037
1.135 ± 0.016
2.497 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here