Beihai sobemo-like virus 18
Average proteome isoelectric point is 5.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEB3|A0A1L3KEB3_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 18 OX=1922689 PE=4 SV=1
MM1 pKa = 7.39 LKK3 pKa = 10.12 ISGEE7 pKa = 3.92 AADD10 pKa = 3.9 QDD12 pKa = 3.79 IEE14 pKa = 4.49 VFDD17 pKa = 4.88 ASPVMSRR24 pKa = 11.84 MTPAQRR30 pKa = 11.84 RR31 pKa = 11.84 DD32 pKa = 3.56 KK33 pKa = 10.54 YY34 pKa = 11.72 KK35 pKa = 10.75 MMADD39 pKa = 3.81 CEE41 pKa = 4.38 DD42 pKa = 3.64 VVVPMLQNVCSEE54 pKa = 4.56 DD55 pKa = 3.58 VPPDD59 pKa = 3.48 YY60 pKa = 11.24 LSFVAPNKK68 pKa = 9.85 PLVDD72 pKa = 4.97 EE73 pKa = 4.89 IRR75 pKa = 11.84 SQLGIEE81 pKa = 4.5 FPLLTSDD88 pKa = 3.88 EE89 pKa = 4.57 SFGTLISTKK98 pKa = 9.33 TSSGHH103 pKa = 5.0 VLNPDD108 pKa = 3.46 SSSGHH113 pKa = 5.01 YY114 pKa = 7.02 WAKK117 pKa = 10.47 YY118 pKa = 7.58 GANNGEE124 pKa = 4.15 VFGWSPEE131 pKa = 3.71 KK132 pKa = 10.7 ARR134 pKa = 11.84 YY135 pKa = 7.28 TGEE138 pKa = 4.0 NRR140 pKa = 11.84 LSDD143 pKa = 3.96 ANMIALFRR151 pKa = 11.84 EE152 pKa = 4.52 KK153 pKa = 10.37 VVSRR157 pKa = 11.84 MVEE160 pKa = 4.09 LYY162 pKa = 10.25 FMPVSDD168 pKa = 4.43 VIKK171 pKa = 10.65 VFIKK175 pKa = 10.63 HH176 pKa = 5.9 EE177 pKa = 3.83 PHH179 pKa = 6.61 KK180 pKa = 10.33 PEE182 pKa = 4.58 KK183 pKa = 10.25 VKK185 pKa = 10.48 QGKK188 pKa = 7.33 WRR190 pKa = 11.84 LIMGVCPTDD199 pKa = 3.36 QICAHH204 pKa = 6.81 LLFDD208 pKa = 3.67 TVLAIFEE215 pKa = 4.93 SIPLLSGTAIGWSPMMEE232 pKa = 4.21 GGVSLYY238 pKa = 10.02 RR239 pKa = 11.84 AYY241 pKa = 11.02 VGDD244 pKa = 4.16 YY245 pKa = 11.64 NMVTADD251 pKa = 3.68 RR252 pKa = 11.84 SSWDD256 pKa = 2.97 WTVQFWLALTFISLMLYY273 pKa = 10.23 LCNTRR278 pKa = 11.84 SLLRR282 pKa = 11.84 QRR284 pKa = 11.84 IITNHH289 pKa = 5.69 ILSMFGSRR297 pKa = 11.84 KK298 pKa = 9.42 FNVGASTFYY307 pKa = 9.88 MHH309 pKa = 7.38 ILGVLLSGWKK319 pKa = 8.16 LTAFCNSLMQIICHH333 pKa = 6.2 HH334 pKa = 6.58 LACLAVGFQLPPPICMGDD352 pKa = 3.42 DD353 pKa = 3.52 TLQNDD358 pKa = 5.33 PITYY362 pKa = 8.5 MRR364 pKa = 11.84 LFSNVLDD371 pKa = 4.6 DD372 pKa = 4.2 VDD374 pKa = 4.05 PVAVDD379 pKa = 3.43 RR380 pKa = 11.84 EE381 pKa = 4.27 YY382 pKa = 10.78 WKK384 pKa = 10.78 FLSSTGAILKK394 pKa = 9.2 MVDD397 pKa = 2.87 RR398 pKa = 11.84 FEE400 pKa = 5.78 DD401 pKa = 3.78 YY402 pKa = 11.35 SEE404 pKa = 4.28 FCGFRR409 pKa = 11.84 FIPGEE414 pKa = 4.33 CYY416 pKa = 9.22 PVYY419 pKa = 10.21 DD420 pKa = 3.95 VKK422 pKa = 11.12 HH423 pKa = 5.8 AVALHH428 pKa = 5.31 NVPSEE433 pKa = 4.08 FLHH436 pKa = 5.33 STLRR440 pKa = 11.84 SYY442 pKa = 11.36 LWLYY446 pKa = 10.9 CYY448 pKa = 10.72 DD449 pKa = 3.65 KK450 pKa = 11.05 EE451 pKa = 4.75 KK452 pKa = 11.53 YY453 pKa = 8.49 EE454 pKa = 4.3 ALEE457 pKa = 3.61 RR458 pKa = 11.84 WLRR461 pKa = 11.84 QTGGSTSFTRR471 pKa = 11.84 LKK473 pKa = 10.42 ILSQINGVAPPCIRR487 pKa = 11.84 PLTWAA492 pKa = 4.13
Molecular weight: 55.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.603
IPC2_protein 5.652
IPC_protein 5.703
Toseland 6.02
ProMoST 6.046
Dawson 5.957
Bjellqvist 5.97
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.135
Solomon 5.957
Lehninger 5.944
Nozaki 6.224
DTASelect 6.415
Thurlkill 6.402
EMBOSS 6.376
Sillero 6.326
Patrickios 3.745
IPC_peptide 5.97
IPC2_peptide 6.313
IPC2.peptide.svr19 6.251
Protein with the highest isoelectric point:
>tr|A0A1L3KEB3|A0A1L3KEB3_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 18 OX=1922689 PE=4 SV=1
MM1 pKa = 6.72 NTSALFAEE9 pKa = 4.43 FLEE12 pKa = 4.09 YY13 pKa = 10.75 RR14 pKa = 11.84 KK15 pKa = 9.01 MRR17 pKa = 11.84 RR18 pKa = 11.84 TMFQSSPLFEE28 pKa = 4.12 WFEE31 pKa = 4.01 LFIVKK36 pKa = 10.28 AEE38 pKa = 4.11 SLSRR42 pKa = 11.84 MFDD45 pKa = 2.99 IGKK48 pKa = 8.08 MIPMALTVSSLSLSLCMMLHH68 pKa = 6.74 GLALLLNVRR77 pKa = 11.84 SRR79 pKa = 11.84 ITSFLSSIFRR89 pKa = 11.84 TLRR92 pKa = 11.84 YY93 pKa = 9.86 VLTRR97 pKa = 11.84 LLPGVRR103 pKa = 11.84 TYY105 pKa = 10.51 LTVIALVVLCLGTLHH120 pKa = 7.3 IALEE124 pKa = 4.36 CFDD127 pKa = 4.3 AFFVEE132 pKa = 5.54 VPDD135 pKa = 3.85 EE136 pKa = 4.16 MEE138 pKa = 4.45 PPVDD142 pKa = 3.34 ISDD145 pKa = 3.58 VSVATVSVLLICVGSYY161 pKa = 11.39 ALLIWTNLDD170 pKa = 3.66 RR171 pKa = 11.84 LVLDD175 pKa = 3.46 YY176 pKa = 11.28 SYY178 pKa = 11.73 VSSEE182 pKa = 3.82 AAKK185 pKa = 10.7 QIDD188 pKa = 4.4 PNLMPGSVAMNDD200 pKa = 3.86 PKK202 pKa = 10.81 WLVTVGARR210 pKa = 11.84 IGNMYY215 pKa = 9.25 TIVGQAFQINQSYY228 pKa = 9.98 VEE230 pKa = 4.04 DD231 pKa = 4.8 EE232 pKa = 3.72 IWFITARR239 pKa = 11.84 HH240 pKa = 4.87 VVNHH244 pKa = 6.22 IKK246 pKa = 10.55 SYY248 pKa = 11.19 DD249 pKa = 3.61 NSIVTILTSTGKK261 pKa = 9.56 PLTPIKK267 pKa = 10.08 QVQLSDD273 pKa = 4.0 SLPDD277 pKa = 3.55 CMLLIVARR285 pKa = 11.84 PKK287 pKa = 10.17 GWNEE291 pKa = 3.66 VGLKK295 pKa = 10.27 VVDD298 pKa = 4.15 PPFPSLSTDD307 pKa = 4.16 FVCPVSIYY315 pKa = 10.97 ARR317 pKa = 11.84 DD318 pKa = 3.6 VPEE321 pKa = 3.9 QFQVRR326 pKa = 11.84 ASRR329 pKa = 11.84 GKK331 pKa = 10.31 AGYY334 pKa = 8.98 LVKK337 pKa = 10.58 NAGMILHH344 pKa = 6.53 HH345 pKa = 6.69 TADD348 pKa = 4.37 TVPGWSGSPILVTGDD363 pKa = 3.25 EE364 pKa = 4.59 GKK366 pKa = 10.81 LEE368 pKa = 3.83 VMGIHH373 pKa = 6.84 LCCSGQTNNASRR385 pKa = 11.84 NMGYY389 pKa = 11.02 ALNAVLEE396 pKa = 4.75 AYY398 pKa = 10.08 VKK400 pKa = 9.42 STYY403 pKa = 10.75 GGVGNTVSQPVKK415 pKa = 10.61 HH416 pKa = 6.02 EE417 pKa = 4.19 LVTEE421 pKa = 3.87 ARR423 pKa = 11.84 GRR425 pKa = 11.84 PRR427 pKa = 11.84 GRR429 pKa = 11.84 PRR431 pKa = 11.84 TRR433 pKa = 11.84 RR434 pKa = 11.84 VKK436 pKa = 10.61 RR437 pKa = 11.84 EE438 pKa = 3.91 NKK440 pKa = 8.89 SKK442 pKa = 10.79 RR443 pKa = 11.84 VFDD446 pKa = 3.7 EE447 pKa = 4.32 LYY449 pKa = 10.67 NRR451 pKa = 11.84 LEE453 pKa = 4.19 SRR455 pKa = 11.84 TGLQISRR462 pKa = 11.84 IDD464 pKa = 3.71 EE465 pKa = 4.09 EE466 pKa = 4.53 GANYY470 pKa = 10.66 DD471 pKa = 4.16 LFEE474 pKa = 4.42 EE475 pKa = 5.12 CSRR478 pKa = 11.84 SLDD481 pKa = 3.4 ATFVNHH487 pKa = 7.18 HH488 pKa = 6.18 EE489 pKa = 4.29 VCQYY493 pKa = 10.53 GCQSPEE499 pKa = 3.72 EE500 pKa = 4.41 CPLCKK505 pKa = 10.34 NQVEE509 pKa = 4.52 ILCGRR514 pKa = 11.84 CDD516 pKa = 3.19 WDD518 pKa = 3.86 DD519 pKa = 4.37 RR520 pKa = 11.84 YY521 pKa = 11.39 AHH523 pKa = 7.36 DD524 pKa = 5.1 EE525 pKa = 4.23 DD526 pKa = 5.66 CAMCNTLTAVCMCITTFGGQVAFANHH552 pKa = 5.56 PTRR555 pKa = 11.84 VDD557 pKa = 3.51 EE558 pKa = 4.6 NGMPEE563 pKa = 4.93 VLLLWFGSDD572 pKa = 3.84 YY573 pKa = 11.37 YY574 pKa = 10.47 PATTVPFSLNIGIDD588 pKa = 3.56 PQPFILHH595 pKa = 5.29 KK596 pKa = 9.78 TIGGEE601 pKa = 3.95 AKK603 pKa = 10.35 GKK605 pKa = 10.34 NKK607 pKa = 10.42 GKK609 pKa = 10.56 GFRR612 pKa = 11.84 NLNKK616 pKa = 10.16 YY617 pKa = 10.09 AGSTSPSVSDD627 pKa = 3.54 PRR629 pKa = 11.84 SFLSWFDD636 pKa = 3.83 DD637 pKa = 4.03 PEE639 pKa = 5.92 ADD641 pKa = 3.21 RR642 pKa = 11.84 TAFSFFPTVLPNKK655 pKa = 9.61 DD656 pKa = 3.34 VMWIVRR662 pKa = 11.84 DD663 pKa = 3.87 NNSGRR668 pKa = 11.84 TSTWEE673 pKa = 3.52 SDD675 pKa = 3.69 YY676 pKa = 11.56 LSDD679 pKa = 5.25 QIIGRR684 pKa = 11.84 LKK686 pKa = 8.39 TWVNAEE692 pKa = 3.98 AKK694 pKa = 10.71 SPVLTKK700 pKa = 10.79 AILDD704 pKa = 3.84 EE705 pKa = 5.09 DD706 pKa = 3.96 DD707 pKa = 4.06 QFIALHH713 pKa = 6.61 DD714 pKa = 5.17 SILEE718 pKa = 4.11 DD719 pKa = 3.36 EE720 pKa = 5.43 FYY722 pKa = 10.82 TVLKK726 pKa = 10.04 VRR728 pKa = 11.84 SVPISDD734 pKa = 3.78 SLEE737 pKa = 4.1 DD738 pKa = 4.1 DD739 pKa = 4.45 RR740 pKa = 11.84 IAVEE744 pKa = 4.94 KK745 pKa = 10.75 ALEE748 pKa = 4.3 DD749 pKa = 3.16 YY750 pKa = 10.34 RR751 pKa = 11.84 YY752 pKa = 11.06 AMEE755 pKa = 4.44 QLGSTYY761 pKa = 10.98 DD762 pKa = 3.61 SLAMRR767 pKa = 11.84 ARR769 pKa = 11.84 LLIDD773 pKa = 4.82 DD774 pKa = 4.56 VSDD777 pKa = 3.88 DD778 pKa = 3.9 AAMISCQLAKK788 pKa = 10.42 DD789 pKa = 3.47 IQYY792 pKa = 10.54 VCDD795 pKa = 4.15 TISNLAQYY803 pKa = 9.61 GAQLTQKK810 pKa = 10.81 SNDD813 pKa = 2.99 ICEE816 pKa = 4.38 CSQVQISKK824 pKa = 10.19 RR825 pKa = 11.84 RR826 pKa = 11.84 LMAKK830 pKa = 9.73 KK831 pKa = 9.44 RR832 pKa = 11.84 AKK834 pKa = 9.74 LRR836 pKa = 11.84 KK837 pKa = 9.1 EE838 pKa = 3.79 VDD840 pKa = 4.08 FQPSQAINDD849 pKa = 3.61 IVSTCRR855 pKa = 11.84 GTVNQALRR863 pKa = 11.84 MAEE866 pKa = 4.3 SIPLRR871 pKa = 11.84 TYY873 pKa = 9.37 RR874 pKa = 11.84 TEE876 pKa = 4.22 SYY878 pKa = 8.41 TAQYY882 pKa = 10.2 PGDD885 pKa = 4.41 PPPNGPSSDD894 pKa = 3.72 QPDD897 pKa = 3.82 KK898 pKa = 10.98 KK899 pKa = 10.69 RR900 pKa = 11.84 KK901 pKa = 8.03 LTEE904 pKa = 3.72 EE905 pKa = 3.91 KK906 pKa = 10.63 LRR908 pKa = 11.84 AGFAKK913 pKa = 10.47 ALNATYY919 pKa = 10.27 DD920 pKa = 3.59 YY921 pKa = 11.53 GILTKK926 pKa = 10.88 LKK928 pKa = 10.42 DD929 pKa = 3.61 FHH931 pKa = 7.06 NEE933 pKa = 3.32 PKK935 pKa = 10.37 TFLDD939 pKa = 3.63 FDD941 pKa = 4.2 KK942 pKa = 11.2 VRR944 pKa = 11.84 QTTSEE949 pKa = 4.2 KK950 pKa = 11.04 EE951 pKa = 3.82 EE952 pKa = 4.72 DD953 pKa = 3.45 SDD955 pKa = 4.12 FSKK958 pKa = 10.88 SASSRR963 pKa = 11.84 LGSPTTPTEE972 pKa = 4.16 SVTPRR977 pKa = 11.84 QFHH980 pKa = 5.43 PTLRR984 pKa = 11.84 SFSRR988 pKa = 11.84 TTLGGSTHH996 pKa = 6.43 PHH998 pKa = 5.11 QMMEE1002 pKa = 4.28 VEE1004 pKa = 5.1 NMAEE1008 pKa = 4.06 RR1009 pKa = 11.84 YY1010 pKa = 7.55 GTLSNYY1016 pKa = 9.37 IPQLPPRR1023 pKa = 11.84 LQKK1026 pKa = 10.54 EE1027 pKa = 4.19 FQTLLNGKK1035 pKa = 9.01 HH1036 pKa = 5.76 SEE1038 pKa = 3.8 EE1039 pKa = 4.0 SRR1041 pKa = 11.84 YY1042 pKa = 9.99 LRR1044 pKa = 11.84 PEE1046 pKa = 3.71 GLMRR1050 pKa = 11.84 SCPTKK1055 pKa = 10.98 LEE1057 pKa = 4.18 ADD1059 pKa = 3.92 SLRR1062 pKa = 11.84 GLLVSLKK1069 pKa = 10.77 DD1070 pKa = 3.51 NAYY1073 pKa = 10.51 LSQEE1077 pKa = 3.98 LMSEE1081 pKa = 4.18 LMRR1084 pKa = 11.84 RR1085 pKa = 11.84 SEE1087 pKa = 3.98 NN1088 pKa = 3.36
Molecular weight: 122.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.615
IPC2_protein 5.652
IPC_protein 5.69
Toseland 5.919
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.931
Wikipedia 5.868
Rodwell 5.855
Grimsley 5.995
Solomon 5.881
Lehninger 5.868
Nozaki 6.135
DTASelect 6.313
Thurlkill 6.275
EMBOSS 6.237
Sillero 6.224
Patrickios 4.101
IPC_peptide 5.906
IPC2_peptide 6.211
IPC2.peptide.svr19 6.227
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1580
492
1088
790.0
89.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.013 ± 0.167
2.405 ± 0.123
6.139 ± 0.127
5.696 ± 0.424
4.241 ± 0.436
5.443 ± 0.129
1.962 ± 0.247
5.0 ± 0.253
4.747 ± 0.143
10.127 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.228 ± 0.434
3.797 ± 0.283
5.316 ± 0.089
3.291 ± 0.336
5.823 ± 0.49
8.608 ± 0.142
5.949 ± 0.556
7.089 ± 0.224
1.519 ± 0.477
3.608 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here