Enterobacteria phage ECGD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140G5W8|A0A140G5W8_9CAUD Uncharacterized protein OS=Enterobacteria phage ECGD1 OX=1784948 GN=ECGD1_120 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 5.62IYY4 pKa = 10.51SGEE7 pKa = 3.98GKK9 pKa = 10.71YY10 pKa = 8.23FTVVDD15 pKa = 3.53NGEE18 pKa = 4.17IIIYY22 pKa = 9.93RR23 pKa = 11.84YY24 pKa = 9.49GSEE27 pKa = 3.64WRR29 pKa = 11.84NEE31 pKa = 4.02TGDD34 pKa = 4.02GYY36 pKa = 10.61ILSLLSLINDD46 pKa = 3.42LTEE49 pKa = 3.97EE50 pKa = 4.19LEE52 pKa = 4.27EE53 pKa = 4.31TKK55 pKa = 10.39RR56 pKa = 11.84SYY58 pKa = 11.51DD59 pKa = 3.61DD60 pKa = 3.87QVDD63 pKa = 3.69SLEE66 pKa = 4.92NIISDD71 pKa = 3.96LNEE74 pKa = 3.93EE75 pKa = 4.07VCYY78 pKa = 10.86LSGQVSEE85 pKa = 4.74LTLEE89 pKa = 4.48CEE91 pKa = 4.48DD92 pKa = 4.7LGDD95 pKa = 4.76VIADD99 pKa = 3.52LNEE102 pKa = 4.0RR103 pKa = 11.84LKK105 pKa = 11.13DD106 pKa = 3.47

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140G670|A0A140G670_9CAUD Uncharacterized protein OS=Enterobacteria phage ECGD1 OX=1784948 GN=ECGD1_221 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.35NKK4 pKa = 9.96HH5 pKa = 6.26PYY7 pKa = 9.5YY8 pKa = 10.59KK9 pKa = 10.29LLHH12 pKa = 5.0VLRR15 pKa = 11.84RR16 pKa = 11.84LRR18 pKa = 11.84NKK20 pKa = 9.82QIEE23 pKa = 4.44DD24 pKa = 3.25FRR26 pKa = 11.84HH27 pKa = 5.9FKK29 pKa = 10.06RR30 pKa = 11.84DD31 pKa = 3.08RR32 pKa = 11.84VNRR35 pKa = 11.84QWMDD39 pKa = 2.77HH40 pKa = 6.31RR41 pKa = 11.84FFSLTVRR48 pKa = 11.84KK49 pKa = 9.25FRR51 pKa = 11.84EE52 pKa = 4.29EE53 pKa = 3.62IKK55 pKa = 9.96KK56 pKa = 8.52TDD58 pKa = 3.5QVIAGAKK65 pKa = 9.32RR66 pKa = 11.84AISMSKK72 pKa = 8.1ITGVNHH78 pKa = 6.23MYY80 pKa = 10.65DD81 pKa = 3.84FKK83 pKa = 10.6TDD85 pKa = 3.17SVVPMM90 pKa = 5.09

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

43519

41

958

186.0

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.576 ± 0.148

1.386 ± 0.071

6.616 ± 0.119

7.291 ± 0.218

4.506 ± 0.135

6.535 ± 0.218

1.864 ± 0.082

6.402 ± 0.105

6.992 ± 0.213

8.049 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.086

5.418 ± 0.15

3.369 ± 0.119

3.261 ± 0.095

3.92 ± 0.112

6.016 ± 0.158

6.078 ± 0.213

7.036 ± 0.184

1.484 ± 0.081

4.564 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski