Amino acid dipepetide frequency for Enterobacteria phage ECGD1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.527AlaAla: 4.527 ± 0.402
0.643AlaCys: 0.643 ± 0.127
3.631AlaAsp: 3.631 ± 0.305
4.366AlaGlu: 4.366 ± 0.408
2.344AlaPhe: 2.344 ± 0.217
4.642AlaGly: 4.642 ± 0.303
1.333AlaHis: 1.333 ± 0.187
3.906AlaIle: 3.906 ± 0.35
5.722AlaLys: 5.722 ± 0.374
5.814AlaLeu: 5.814 ± 0.333
2.114AlaMet: 2.114 ± 0.273
3.194AlaAsn: 3.194 ± 0.319
1.838AlaPro: 1.838 ± 0.227
2.482AlaGln: 2.482 ± 0.248
2.482AlaArg: 2.482 ± 0.273
4.343AlaSer: 4.343 ± 0.319
4.481AlaThr: 4.481 ± 0.39
3.837AlaVal: 3.837 ± 0.356
1.218AlaTrp: 1.218 ± 0.221
2.849AlaTyr: 2.849 ± 0.262
0.0AlaXaa: 0.0 ± 0.0
Cys
0.919CysAla: 0.919 ± 0.137
0.299CysCys: 0.299 ± 0.107
0.942CysAsp: 0.942 ± 0.162
0.689CysGlu: 0.689 ± 0.16
1.034CysPhe: 1.034 ± 0.156
0.919CysGly: 0.919 ± 0.138
0.207CysHis: 0.207 ± 0.075
0.942CysIle: 0.942 ± 0.114
0.919CysLys: 0.919 ± 0.141
1.264CysLeu: 1.264 ± 0.161
0.207CysMet: 0.207 ± 0.07
0.597CysAsn: 0.597 ± 0.1
0.574CysPro: 0.574 ± 0.15
0.391CysGln: 0.391 ± 0.092
0.574CysArg: 0.574 ± 0.122
0.758CysSer: 0.758 ± 0.144
0.643CysThr: 0.643 ± 0.109
0.919CysVal: 0.919 ± 0.17
0.276CysTrp: 0.276 ± 0.077
0.781CysTyr: 0.781 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
4.113AspAla: 4.113 ± 0.324
0.62AspCys: 0.62 ± 0.117
3.792AspAsp: 3.792 ± 0.304
3.86AspGlu: 3.86 ± 0.361
3.263AspPhe: 3.263 ± 0.291
5.101AspGly: 5.101 ± 0.4
1.356AspHis: 1.356 ± 0.188
4.665AspIle: 4.665 ± 0.303
4.481AspLys: 4.481 ± 0.336
5.768AspLeu: 5.768 ± 0.373
1.792AspMet: 1.792 ± 0.19
3.883AspAsn: 3.883 ± 0.358
2.62AspPro: 2.62 ± 0.26
1.953AspGln: 1.953 ± 0.228
2.528AspArg: 2.528 ± 0.285
3.286AspSer: 3.286 ± 0.269
3.539AspThr: 3.539 ± 0.299
4.757AspVal: 4.757 ± 0.308
1.195AspTrp: 1.195 ± 0.183
3.585AspTyr: 3.585 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
4.94GluAla: 4.94 ± 0.374
1.08GluCys: 1.08 ± 0.157
5.814GluAsp: 5.814 ± 0.373
7.974GluGlu: 7.974 ± 0.645
2.872GluPhe: 2.872 ± 0.261
4.113GluGly: 4.113 ± 0.305
1.448GluHis: 1.448 ± 0.193
4.78GluIle: 4.78 ± 0.343
5.262GluLys: 5.262 ± 0.321
5.101GluLeu: 5.101 ± 0.364
2.436GluMet: 2.436 ± 0.237
3.746GluAsn: 3.746 ± 0.329
1.563GluPro: 1.563 ± 0.21
2.643GluGln: 2.643 ± 0.241
3.01GluArg: 3.01 ± 0.276
3.286GluSer: 3.286 ± 0.295
3.723GluThr: 3.723 ± 0.315
4.895GluVal: 4.895 ± 0.359
1.563GluTrp: 1.563 ± 0.201
2.666GluTyr: 2.666 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.918PheAla: 2.918 ± 0.269
0.735PheCys: 0.735 ± 0.134
3.631PheAsp: 3.631 ± 0.333
2.597PheGlu: 2.597 ± 0.253
2.275PhePhe: 2.275 ± 0.265
2.872PheGly: 2.872 ± 0.276
0.643PheHis: 0.643 ± 0.126
2.735PheIle: 2.735 ± 0.217
3.263PheLys: 3.263 ± 0.268
3.493PheLeu: 3.493 ± 0.307
1.103PheMet: 1.103 ± 0.141
2.482PheAsn: 2.482 ± 0.211
1.402PhePro: 1.402 ± 0.18
1.471PheGln: 1.471 ± 0.199
1.7PheArg: 1.7 ± 0.22
3.378PheSer: 3.378 ± 0.291
2.803PheThr: 2.803 ± 0.265
3.01PheVal: 3.01 ± 0.291
0.62PheTrp: 0.62 ± 0.11
1.93PheTyr: 1.93 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
3.723GlyAla: 3.723 ± 0.27
1.08GlyCys: 1.08 ± 0.163
4.849GlyAsp: 4.849 ± 0.449
4.527GlyGlu: 4.527 ± 0.289
3.562GlyPhe: 3.562 ± 0.297
4.596GlyGly: 4.596 ± 0.543
1.264GlyHis: 1.264 ± 0.156
4.021GlyIle: 4.021 ± 0.358
4.895GlyLys: 4.895 ± 0.336
5.009GlyLeu: 5.009 ± 0.363
1.7GlyMet: 1.7 ± 0.197
3.516GlyAsn: 3.516 ± 0.342
0.414GlyPro: 0.414 ± 0.133
1.999GlyGln: 1.999 ± 0.234
2.528GlyArg: 2.528 ± 0.255
3.998GlySer: 3.998 ± 0.322
3.7GlyThr: 3.7 ± 0.424
5.538GlyVal: 5.538 ± 0.349
1.034GlyTrp: 1.034 ± 0.187
3.401GlyTyr: 3.401 ± 0.275
0.0GlyXaa: 0.0 ± 0.0
His
1.08HisAla: 1.08 ± 0.161
0.391HisCys: 0.391 ± 0.089
1.172HisAsp: 1.172 ± 0.158
0.85HisGlu: 0.85 ± 0.142
1.126HisPhe: 1.126 ± 0.158
1.333HisGly: 1.333 ± 0.185
0.207HisHis: 0.207 ± 0.077
1.333HisIle: 1.333 ± 0.198
1.08HisLys: 1.08 ± 0.15
1.448HisLeu: 1.448 ± 0.194
0.597HisMet: 0.597 ± 0.135
1.241HisAsn: 1.241 ± 0.167
0.873HisPro: 0.873 ± 0.151
0.414HisGln: 0.414 ± 0.091
0.965HisArg: 0.965 ± 0.155
0.988HisSer: 0.988 ± 0.143
1.057HisThr: 1.057 ± 0.166
1.218HisVal: 1.218 ± 0.173
0.276HisTrp: 0.276 ± 0.079
0.988HisTyr: 0.988 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
4.527IleAla: 4.527 ± 0.314
0.965IleCys: 0.965 ± 0.16
4.205IleAsp: 4.205 ± 0.34
4.757IleGlu: 4.757 ± 0.371
2.436IlePhe: 2.436 ± 0.257
3.746IleGly: 3.746 ± 0.322
1.057IleHis: 1.057 ± 0.167
3.906IleIle: 3.906 ± 0.301
4.205IleLys: 4.205 ± 0.252
4.826IleLeu: 4.826 ± 0.32
1.149IleMet: 1.149 ± 0.163
3.837IleAsn: 3.837 ± 0.323
3.01IlePro: 3.01 ± 0.279
2.39IleGln: 2.39 ± 0.222
2.367IleArg: 2.367 ± 0.226
4.044IleSer: 4.044 ± 0.311
4.251IleThr: 4.251 ± 0.32
5.216IleVal: 5.216 ± 0.322
0.597IleTrp: 0.597 ± 0.113
2.528IleTyr: 2.528 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
5.469LysAla: 5.469 ± 0.393
0.735LysCys: 0.735 ± 0.124
4.688LysAsp: 4.688 ± 0.378
6.434LysGlu: 6.434 ± 0.434
2.597LysPhe: 2.597 ± 0.254
4.527LysGly: 4.527 ± 0.471
1.7LysHis: 1.7 ± 0.165
4.32LysIle: 4.32 ± 0.323
4.297LysLys: 4.297 ± 0.445
5.4LysLeu: 5.4 ± 0.354
2.367LysMet: 2.367 ± 0.224
3.401LysAsn: 3.401 ± 0.302
2.091LysPro: 2.091 ± 0.22
2.666LysGln: 2.666 ± 0.253
3.263LysArg: 3.263 ± 0.342
3.7LysSer: 3.7 ± 0.302
3.723LysThr: 3.723 ± 0.3
4.458LysVal: 4.458 ± 0.288
1.195LysTrp: 1.195 ± 0.146
2.895LysTyr: 2.895 ± 0.302
0.0LysXaa: 0.0 ± 0.0
Leu
5.63LeuAla: 5.63 ± 0.366
1.011LeuCys: 1.011 ± 0.168
5.354LeuAsp: 5.354 ± 0.293
5.906LeuGlu: 5.906 ± 0.494
3.263LeuPhe: 3.263 ± 0.3
4.872LeuGly: 4.872 ± 0.312
1.471LeuHis: 1.471 ± 0.2
4.435LeuIle: 4.435 ± 0.372
5.653LeuLys: 5.653 ± 0.371
6.342LeuLeu: 6.342 ± 0.387
1.884LeuMet: 1.884 ± 0.22
4.366LeuAsn: 4.366 ± 0.344
3.079LeuPro: 3.079 ± 0.253
2.757LeuGln: 2.757 ± 0.252
3.631LeuArg: 3.631 ± 0.307
5.883LeuSer: 5.883 ± 0.326
5.584LeuThr: 5.584 ± 0.417
5.17LeuVal: 5.17 ± 0.334
0.919LeuTrp: 0.919 ± 0.144
3.286LeuTyr: 3.286 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
1.999MetAla: 1.999 ± 0.165
0.391MetCys: 0.391 ± 0.093
1.08MetAsp: 1.08 ± 0.145
2.229MetGlu: 2.229 ± 0.238
1.195MetPhe: 1.195 ± 0.179
1.402MetGly: 1.402 ± 0.183
0.551MetHis: 0.551 ± 0.117
2.045MetIle: 2.045 ± 0.226
1.999MetLys: 1.999 ± 0.186
2.505MetLeu: 2.505 ± 0.233
0.689MetMet: 0.689 ± 0.143
0.919MetAsn: 0.919 ± 0.183
0.804MetPro: 0.804 ± 0.189
0.965MetGln: 0.965 ± 0.14
1.034MetArg: 1.034 ± 0.156
1.953MetSer: 1.953 ± 0.186
1.563MetThr: 1.563 ± 0.194
1.7MetVal: 1.7 ± 0.199
0.414MetTrp: 0.414 ± 0.116
0.942MetTyr: 0.942 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
3.286AsnAla: 3.286 ± 0.249
0.666AsnCys: 0.666 ± 0.112
2.436AsnAsp: 2.436 ± 0.268
3.079AsnGlu: 3.079 ± 0.287
2.666AsnPhe: 2.666 ± 0.285
4.136AsnGly: 4.136 ± 0.316
0.965AsnHis: 0.965 ± 0.141
4.642AsnIle: 4.642 ± 0.336
3.906AsnLys: 3.906 ± 0.32
3.837AsnLeu: 3.837 ± 0.251
1.609AsnMet: 1.609 ± 0.226
3.217AsnAsn: 3.217 ± 0.332
2.436AsnPro: 2.436 ± 0.256
1.494AsnGln: 1.494 ± 0.184
1.861AsnArg: 1.861 ± 0.221
3.171AsnSer: 3.171 ± 0.277
4.021AsnThr: 4.021 ± 0.381
3.562AsnVal: 3.562 ± 0.286
0.643AsnTrp: 0.643 ± 0.154
2.551AsnTyr: 2.551 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
2.16ProAla: 2.16 ± 0.211
0.529ProCys: 0.529 ± 0.105
2.551ProAsp: 2.551 ± 0.267
3.194ProGlu: 3.194 ± 0.225
1.792ProPhe: 1.792 ± 0.187
1.746ProGly: 1.746 ± 0.248
0.597ProHis: 0.597 ± 0.108
1.609ProIle: 1.609 ± 0.201
2.114ProLys: 2.114 ± 0.217
2.045ProLeu: 2.045 ± 0.252
0.689ProMet: 0.689 ± 0.129
2.068ProAsn: 2.068 ± 0.258
0.919ProPro: 0.919 ± 0.159
0.781ProGln: 0.781 ± 0.151
1.011ProArg: 1.011 ± 0.156
2.597ProSer: 2.597 ± 0.259
2.459ProThr: 2.459 ± 0.274
2.689ProVal: 2.689 ± 0.259
0.345ProTrp: 0.345 ± 0.094
1.792ProTyr: 1.792 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
2.252GlnAla: 2.252 ± 0.2
0.597GlnCys: 0.597 ± 0.114
1.953GlnAsp: 1.953 ± 0.176
2.689GlnGlu: 2.689 ± 0.233
1.517GlnPhe: 1.517 ± 0.186
2.344GlnGly: 2.344 ± 0.292
0.666GlnHis: 0.666 ± 0.116
2.229GlnIle: 2.229 ± 0.188
1.907GlnLys: 1.907 ± 0.194
2.436GlnLeu: 2.436 ± 0.272
1.08GlnMet: 1.08 ± 0.169
1.494GlnAsn: 1.494 ± 0.183
0.942GlnPro: 0.942 ± 0.148
1.425GlnGln: 1.425 ± 0.189
1.287GlnArg: 1.287 ± 0.183
1.586GlnSer: 1.586 ± 0.193
1.815GlnThr: 1.815 ± 0.2
2.022GlnVal: 2.022 ± 0.23
0.551GlnTrp: 0.551 ± 0.109
1.815GlnTyr: 1.815 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
2.298ArgAla: 2.298 ± 0.232
0.689ArgCys: 0.689 ± 0.144
2.941ArgAsp: 2.941 ± 0.254
2.597ArgGlu: 2.597 ± 0.267
1.563ArgPhe: 1.563 ± 0.178
2.666ArgGly: 2.666 ± 0.271
0.643ArgHis: 0.643 ± 0.105
2.62ArgIle: 2.62 ± 0.272
3.033ArgLys: 3.033 ± 0.28
3.125ArgLeu: 3.125 ± 0.312
1.195ArgMet: 1.195 ± 0.177
2.367ArgAsn: 2.367 ± 0.231
1.149ArgPro: 1.149 ± 0.171
1.54ArgGln: 1.54 ± 0.244
1.609ArgArg: 1.609 ± 0.245
2.436ArgSer: 2.436 ± 0.211
1.976ArgThr: 1.976 ± 0.206
2.735ArgVal: 2.735 ± 0.282
0.299ArgTrp: 0.299 ± 0.087
1.723ArgTyr: 1.723 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
4.044SerAla: 4.044 ± 0.371
0.735SerCys: 0.735 ± 0.13
3.493SerAsp: 3.493 ± 0.289
3.769SerGlu: 3.769 ± 0.278
2.918SerPhe: 2.918 ± 0.288
4.711SerGly: 4.711 ± 0.394
0.942SerHis: 0.942 ± 0.143
3.654SerIle: 3.654 ± 0.318
4.274SerLys: 4.274 ± 0.276
4.94SerLeu: 4.94 ± 0.356
1.517SerMet: 1.517 ± 0.197
3.516SerAsn: 3.516 ± 0.357
2.528SerPro: 2.528 ± 0.262
1.517SerGln: 1.517 ± 0.217
2.551SerArg: 2.551 ± 0.23
3.929SerSer: 3.929 ± 0.425
3.493SerThr: 3.493 ± 0.354
4.205SerVal: 4.205 ± 0.315
0.758SerTrp: 0.758 ± 0.125
2.666SerTyr: 2.666 ± 0.237
0.0SerXaa: 0.0 ± 0.0
Thr
3.837ThrAla: 3.837 ± 0.444
0.781ThrCys: 0.781 ± 0.129
3.309ThrAsp: 3.309 ± 0.295
4.504ThrGlu: 4.504 ± 0.346
2.987ThrPhe: 2.987 ± 0.254
4.297ThrGly: 4.297 ± 0.366
1.011ThrHis: 1.011 ± 0.172
3.929ThrIle: 3.929 ± 0.314
4.09ThrLys: 4.09 ± 0.267
5.814ThrLeu: 5.814 ± 0.404
1.333ThrMet: 1.333 ± 0.176
2.941ThrAsn: 2.941 ± 0.28
2.849ThrPro: 2.849 ± 0.237
2.091ThrGln: 2.091 ± 0.18
1.953ThrArg: 1.953 ± 0.212
2.895ThrSer: 2.895 ± 0.307
3.631ThrThr: 3.631 ± 0.435
4.619ThrVal: 4.619 ± 0.428
0.988ThrTrp: 0.988 ± 0.155
2.918ThrTyr: 2.918 ± 0.348
0.0ThrXaa: 0.0 ± 0.0
Val
4.136ValAla: 4.136 ± 0.339
1.057ValCys: 1.057 ± 0.183
5.354ValAsp: 5.354 ± 0.344
4.619ValGlu: 4.619 ± 0.338
2.826ValPhe: 2.826 ± 0.327
3.746ValGly: 3.746 ± 0.307
1.379ValHis: 1.379 ± 0.181
4.849ValIle: 4.849 ± 0.35
5.17ValLys: 5.17 ± 0.38
5.63ValLeu: 5.63 ± 0.373
1.402ValMet: 1.402 ± 0.182
3.86ValAsn: 3.86 ± 0.3
2.436ValPro: 2.436 ± 0.243
1.953ValGln: 1.953 ± 0.272
2.459ValArg: 2.459 ± 0.286
4.504ValSer: 4.504 ± 0.401
4.78ValThr: 4.78 ± 0.386
5.538ValVal: 5.538 ± 0.437
0.965ValTrp: 0.965 ± 0.173
3.677ValTyr: 3.677 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
0.574TrpAla: 0.574 ± 0.106
0.184TrpCys: 0.184 ± 0.056
1.402TrpAsp: 1.402 ± 0.186
1.241TrpGlu: 1.241 ± 0.176
0.85TrpPhe: 0.85 ± 0.149
0.735TrpGly: 0.735 ± 0.148
0.46TrpHis: 0.46 ± 0.102
1.057TrpIle: 1.057 ± 0.177
0.942TrpLys: 0.942 ± 0.182
1.586TrpLeu: 1.586 ± 0.218
0.46TrpMet: 0.46 ± 0.11
0.804TrpAsn: 0.804 ± 0.123
0.276TrpPro: 0.276 ± 0.076
0.299TrpGln: 0.299 ± 0.109
0.551TrpArg: 0.551 ± 0.127
0.804TrpSer: 0.804 ± 0.116
0.666TrpThr: 0.666 ± 0.137
1.034TrpVal: 1.034 ± 0.15
0.299TrpTrp: 0.299 ± 0.09
0.62TrpTyr: 0.62 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.332TyrAla: 3.332 ± 0.305
0.666TyrCys: 0.666 ± 0.117
3.562TyrAsp: 3.562 ± 0.297
3.033TyrGlu: 3.033 ± 0.286
1.976TyrPhe: 1.976 ± 0.207
2.849TyrGly: 2.849 ± 0.245
0.735TyrHis: 0.735 ± 0.119
2.344TyrIle: 2.344 ± 0.225
2.712TyrLys: 2.712 ± 0.263
4.113TyrLeu: 4.113 ± 0.289
1.034TyrMet: 1.034 ± 0.136
2.735TyrAsn: 2.735 ± 0.281
1.884TyrPro: 1.884 ± 0.196
1.379TyrGln: 1.379 ± 0.199
1.838TyrArg: 1.838 ± 0.195
2.62TyrSer: 2.62 ± 0.276
2.872TyrThr: 2.872 ± 0.265
3.24TyrVal: 3.24 ± 0.26
0.689TyrTrp: 0.689 ± 0.122
2.022TyrTyr: 2.022 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 234 proteins (43519 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski