Amino acid dipepetide frequency for Erythrobacter sp. NAP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.359AlaAla: 15.359 ± 0.157
1.051AlaCys: 1.051 ± 0.036
6.943AlaAsp: 6.943 ± 0.081
8.237AlaGlu: 8.237 ± 0.09
4.432AlaPhe: 4.432 ± 0.074
10.236AlaGly: 10.236 ± 0.128
2.105AlaHis: 2.105 ± 0.05
6.902AlaIle: 6.902 ± 0.102
4.406AlaLys: 4.406 ± 0.09
12.91AlaLeu: 12.91 ± 0.177
3.77AlaMet: 3.77 ± 0.066
3.518AlaAsn: 3.518 ± 0.062
5.517AlaPro: 5.517 ± 0.084
4.525AlaGln: 4.525 ± 0.074
8.338AlaArg: 8.338 ± 0.126
7.275AlaSer: 7.275 ± 0.113
5.656AlaThr: 5.656 ± 0.103
7.634AlaVal: 7.634 ± 0.108
1.364AlaTrp: 1.364 ± 0.04
2.44AlaTyr: 2.44 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.976CysAla: 0.976 ± 0.034
0.083CysCys: 0.083 ± 0.01
0.595CysAsp: 0.595 ± 0.024
0.581CysGlu: 0.581 ± 0.027
0.293CysPhe: 0.293 ± 0.019
0.83CysGly: 0.83 ± 0.033
0.217CysHis: 0.217 ± 0.02
0.369CysIle: 0.369 ± 0.021
0.206CysLys: 0.206 ± 0.015
0.678CysLeu: 0.678 ± 0.034
0.141CysMet: 0.141 ± 0.013
0.244CysAsn: 0.244 ± 0.016
0.388CysPro: 0.388 ± 0.021
0.197CysGln: 0.197 ± 0.013
0.459CysArg: 0.459 ± 0.022
0.448CysSer: 0.448 ± 0.025
0.391CysThr: 0.391 ± 0.021
0.558CysVal: 0.558 ± 0.026
0.11CysTrp: 0.11 ± 0.011
0.166CysTyr: 0.166 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
7.511AspAla: 7.511 ± 0.09
0.531AspCys: 0.531 ± 0.026
3.741AspAsp: 3.741 ± 0.067
4.741AspGlu: 4.741 ± 0.078
2.445AspPhe: 2.445 ± 0.057
5.771AspGly: 5.771 ± 0.097
1.246AspHis: 1.246 ± 0.041
3.163AspIle: 3.163 ± 0.064
1.851AspLys: 1.851 ± 0.054
5.809AspLeu: 5.809 ± 0.083
1.482AspMet: 1.482 ± 0.041
1.733AspAsn: 1.733 ± 0.047
3.725AspPro: 3.725 ± 0.057
1.869AspGln: 1.869 ± 0.053
4.202AspArg: 4.202 ± 0.066
2.354AspSer: 2.354 ± 0.053
2.966AspThr: 2.966 ± 0.06
4.08AspVal: 4.08 ± 0.058
1.153AspTrp: 1.153 ± 0.034
1.606AspTyr: 1.606 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
8.951GluAla: 8.951 ± 0.119
0.448GluCys: 0.448 ± 0.023
4.02GluAsp: 4.02 ± 0.077
4.966GluGlu: 4.966 ± 0.089
2.218GluPhe: 2.218 ± 0.049
5.629GluGly: 5.629 ± 0.087
1.284GluHis: 1.284 ± 0.039
3.592GluIle: 3.592 ± 0.058
2.455GluLys: 2.455 ± 0.062
6.219GluLeu: 6.219 ± 0.081
1.874GluMet: 1.874 ± 0.047
1.981GluAsn: 1.981 ± 0.045
3.049GluPro: 3.049 ± 0.061
2.574GluGln: 2.574 ± 0.054
5.548GluArg: 5.548 ± 0.081
2.84GluSer: 2.84 ± 0.058
3.757GluThr: 3.757 ± 0.061
4.441GluVal: 4.441 ± 0.071
1.004GluTrp: 1.004 ± 0.034
1.336GluTyr: 1.336 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
5.101PheAla: 5.101 ± 0.077
0.323PheCys: 0.323 ± 0.017
2.928PheAsp: 2.928 ± 0.067
2.713PheGlu: 2.713 ± 0.051
1.527PhePhe: 1.527 ± 0.048
3.849PheGly: 3.849 ± 0.077
0.669PheHis: 0.669 ± 0.024
1.703PheIle: 1.703 ± 0.046
1.002PheLys: 1.002 ± 0.033
3.139PheLeu: 3.139 ± 0.055
0.833PheMet: 0.833 ± 0.029
1.18PheAsn: 1.18 ± 0.041
1.528PhePro: 1.528 ± 0.044
0.962PheGln: 0.962 ± 0.028
2.027PheArg: 2.027 ± 0.051
2.255PheSer: 2.255 ± 0.055
2.169PheThr: 2.169 ± 0.058
2.8PheVal: 2.8 ± 0.056
0.586PheTrp: 0.586 ± 0.026
0.955PheTyr: 0.955 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
9.252GlyAla: 9.252 ± 0.11
0.741GlyCys: 0.741 ± 0.027
5.396GlyAsp: 5.396 ± 0.101
6.396GlyGlu: 6.396 ± 0.083
3.781GlyPhe: 3.781 ± 0.075
8.004GlyGly: 8.004 ± 0.209
1.705GlyHis: 1.705 ± 0.046
4.564GlyIle: 4.564 ± 0.073
3.231GlyLys: 3.231 ± 0.064
8.215GlyLeu: 8.215 ± 0.1
2.38GlyMet: 2.38 ± 0.049
2.53GlyAsn: 2.53 ± 0.068
3.458GlyPro: 3.458 ± 0.066
2.808GlyGln: 2.808 ± 0.059
5.413GlyArg: 5.413 ± 0.085
5.329GlySer: 5.329 ± 0.104
4.828GlyThr: 4.828 ± 0.093
5.959GlyVal: 5.959 ± 0.098
1.453GlyTrp: 1.453 ± 0.044
2.147GlyTyr: 2.147 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.101HisAla: 2.101 ± 0.054
0.25HisCys: 0.25 ± 0.016
1.201HisAsp: 1.201 ± 0.038
1.165HisGlu: 1.165 ± 0.037
0.853HisPhe: 0.853 ± 0.03
1.752HisGly: 1.752 ± 0.055
0.502HisHis: 0.502 ± 0.03
0.849HisIle: 0.849 ± 0.029
0.548HisLys: 0.548 ± 0.03
1.679HisLeu: 1.679 ± 0.04
0.431HisMet: 0.431 ± 0.024
0.495HisAsn: 0.495 ± 0.022
1.124HisPro: 1.124 ± 0.04
0.489HisGln: 0.489 ± 0.024
1.245HisArg: 1.245 ± 0.044
1.045HisSer: 1.045 ± 0.039
0.8HisThr: 0.8 ± 0.029
1.265HisVal: 1.265 ± 0.038
0.334HisTrp: 0.334 ± 0.02
0.517HisTyr: 0.517 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.908IleAla: 7.908 ± 0.121
0.439IleCys: 0.439 ± 0.023
3.819IleAsp: 3.819 ± 0.06
4.327IleGlu: 4.327 ± 0.067
1.737IlePhe: 1.737 ± 0.046
5.1IleGly: 5.1 ± 0.086
0.866IleHis: 0.866 ± 0.034
2.321IleIle: 2.321 ± 0.057
1.41IleLys: 1.41 ± 0.051
3.931IleLeu: 3.931 ± 0.064
0.99IleMet: 0.99 ± 0.036
1.486IleAsn: 1.486 ± 0.039
2.331IlePro: 2.331 ± 0.049
1.216IleGln: 1.216 ± 0.038
2.949IleArg: 2.949 ± 0.066
2.918IleSer: 2.918 ± 0.057
2.919IleThr: 2.919 ± 0.054
3.873IleVal: 3.873 ± 0.067
0.67IleTrp: 0.67 ± 0.027
1.111IleTyr: 1.111 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
3.999LysAla: 3.999 ± 0.079
0.179LysCys: 0.179 ± 0.014
1.683LysAsp: 1.683 ± 0.046
1.698LysGlu: 1.698 ± 0.054
0.915LysPhe: 0.915 ± 0.034
2.641LysGly: 2.641 ± 0.058
0.68LysHis: 0.68 ± 0.03
1.465LysIle: 1.465 ± 0.041
1.329LysLys: 1.329 ± 0.055
3.387LysLeu: 3.387 ± 0.071
0.823LysMet: 0.823 ± 0.036
0.769LysAsn: 0.769 ± 0.025
1.895LysPro: 1.895 ± 0.046
0.986LysGln: 0.986 ± 0.038
2.473LysArg: 2.473 ± 0.058
1.815LysSer: 1.815 ± 0.05
1.785LysThr: 1.785 ± 0.042
2.304LysVal: 2.304 ± 0.058
0.42LysTrp: 0.42 ± 0.017
0.637LysTyr: 0.637 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
13.255LeuAla: 13.255 ± 0.161
0.716LeuCys: 0.716 ± 0.028
6.004LeuAsp: 6.004 ± 0.09
6.073LeuGlu: 6.073 ± 0.083
3.653LeuPhe: 3.653 ± 0.072
8.219LeuGly: 8.219 ± 0.103
1.605LeuHis: 1.605 ± 0.041
4.872LeuIle: 4.872 ± 0.07
3.043LeuLys: 3.043 ± 0.068
8.547LeuLeu: 8.547 ± 0.123
2.161LeuMet: 2.161 ± 0.055
2.357LeuAsn: 2.357 ± 0.044
4.986LeuPro: 4.986 ± 0.074
2.369LeuGln: 2.369 ± 0.053
5.927LeuArg: 5.927 ± 0.071
6.068LeuSer: 6.068 ± 0.079
5.488LeuThr: 5.488 ± 0.084
6.983LeuVal: 6.983 ± 0.099
1.126LeuTrp: 1.126 ± 0.04
1.909LeuTyr: 1.909 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.239MetAla: 3.239 ± 0.059
0.158MetCys: 0.158 ± 0.014
1.308MetAsp: 1.308 ± 0.041
1.343MetGlu: 1.343 ± 0.04
0.733MetPhe: 0.733 ± 0.03
1.973MetGly: 1.973 ± 0.047
0.476MetHis: 0.476 ± 0.022
1.413MetIle: 1.413 ± 0.041
0.996MetLys: 0.996 ± 0.035
2.596MetLeu: 2.596 ± 0.05
0.723MetMet: 0.723 ± 0.031
0.791MetAsn: 0.791 ± 0.027
1.406MetPro: 1.406 ± 0.045
0.811MetGln: 0.811 ± 0.029
1.763MetArg: 1.763 ± 0.045
1.676MetSer: 1.676 ± 0.045
1.612MetThr: 1.612 ± 0.039
1.709MetVal: 1.709 ± 0.044
0.254MetTrp: 0.254 ± 0.016
0.297MetTyr: 0.297 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.443AsnAla: 3.443 ± 0.065
0.274AsnCys: 0.274 ± 0.016
1.627AsnAsp: 1.627 ± 0.035
1.579AsnGlu: 1.579 ± 0.044
1.163AsnPhe: 1.163 ± 0.037
2.558AsnGly: 2.558 ± 0.068
0.525AsnHis: 0.525 ± 0.027
1.412AsnIle: 1.412 ± 0.036
0.691AsnLys: 0.691 ± 0.027
2.628AsnLeu: 2.628 ± 0.058
0.629AsnMet: 0.629 ± 0.027
0.708AsnAsn: 0.708 ± 0.035
1.946AsnPro: 1.946 ± 0.044
0.86AsnGln: 0.86 ± 0.029
1.946AsnArg: 1.946 ± 0.048
1.529AsnSer: 1.529 ± 0.045
1.382AsnThr: 1.382 ± 0.043
2.051AsnVal: 2.051 ± 0.054
0.458AsnTrp: 0.458 ± 0.021
0.64AsnTyr: 0.64 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
5.696ProAla: 5.696 ± 0.087
0.294ProCys: 0.294 ± 0.018
3.695ProAsp: 3.695 ± 0.065
4.138ProGlu: 4.138 ± 0.071
1.954ProPhe: 1.954 ± 0.04
4.349ProGly: 4.349 ± 0.068
0.951ProHis: 0.951 ± 0.03
2.465ProIle: 2.465 ± 0.05
1.551ProLys: 1.551 ± 0.044
4.469ProLeu: 4.469 ± 0.074
1.17ProMet: 1.17 ± 0.036
1.349ProAsn: 1.349 ± 0.035
2.482ProPro: 2.482 ± 0.095
1.784ProGln: 1.784 ± 0.042
2.601ProArg: 2.601 ± 0.052
2.811ProSer: 2.811 ± 0.063
2.469ProThr: 2.469 ± 0.054
3.865ProVal: 3.865 ± 0.071
0.592ProTrp: 0.592 ± 0.021
1.019ProTyr: 1.019 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.687GlnAla: 3.687 ± 0.063
0.242GlnCys: 0.242 ± 0.018
1.634GlnAsp: 1.634 ± 0.046
1.585GlnGlu: 1.585 ± 0.043
1.306GlnPhe: 1.306 ± 0.042
2.453GlnGly: 2.453 ± 0.049
0.609GlnHis: 0.609 ± 0.024
1.868GlnIle: 1.868 ± 0.05
0.865GlnLys: 0.865 ± 0.031
3.146GlnLeu: 3.146 ± 0.06
0.953GlnMet: 0.953 ± 0.029
0.865GlnAsn: 0.865 ± 0.031
1.695GlnPro: 1.695 ± 0.046
1.14GlnGln: 1.14 ± 0.043
2.358GlnArg: 2.358 ± 0.055
2.028GlnSer: 2.028 ± 0.054
1.689GlnThr: 1.689 ± 0.052
2.364GlnVal: 2.364 ± 0.052
0.442GlnTrp: 0.442 ± 0.022
0.7GlnTyr: 0.7 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
7.41ArgAla: 7.41 ± 0.102
0.452ArgCys: 0.452 ± 0.025
4.053ArgAsp: 4.053 ± 0.073
4.971ArgGlu: 4.971 ± 0.073
3.011ArgPhe: 3.011 ± 0.069
4.705ArgGly: 4.705 ± 0.078
1.285ArgHis: 1.285 ± 0.037
3.948ArgIle: 3.948 ± 0.065
2.203ArgLys: 2.203 ± 0.054
6.809ArgLeu: 6.809 ± 0.106
1.775ArgMet: 1.775 ± 0.046
1.842ArgAsn: 1.842 ± 0.045
2.949ArgPro: 2.949 ± 0.057
2.166ArgGln: 2.166 ± 0.046
4.625ArgArg: 4.625 ± 0.09
3.789ArgSer: 3.789 ± 0.061
3.146ArgThr: 3.146 ± 0.065
4.376ArgVal: 4.376 ± 0.078
1.006ArgTrp: 1.006 ± 0.036
1.598ArgTyr: 1.598 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.682SerAla: 6.682 ± 0.094
0.403SerCys: 0.403 ± 0.021
3.817SerAsp: 3.817 ± 0.055
3.865SerGlu: 3.865 ± 0.069
2.237SerPhe: 2.237 ± 0.058
5.786SerGly: 5.786 ± 0.097
0.989SerHis: 0.989 ± 0.032
2.906SerIle: 2.906 ± 0.066
1.595SerLys: 1.595 ± 0.047
5.439SerLeu: 5.439 ± 0.084
1.377SerMet: 1.377 ± 0.033
1.68SerAsn: 1.68 ± 0.044
2.769SerPro: 2.769 ± 0.053
1.914SerGln: 1.914 ± 0.046
3.58SerArg: 3.58 ± 0.068
3.207SerSer: 3.207 ± 0.08
2.76SerThr: 2.76 ± 0.055
3.884SerVal: 3.884 ± 0.063
0.808SerTrp: 0.808 ± 0.03
1.269SerTyr: 1.269 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
5.951ThrAla: 5.951 ± 0.1
0.423ThrCys: 0.423 ± 0.021
2.957ThrAsp: 2.957 ± 0.063
2.836ThrGlu: 2.836 ± 0.057
2.021ThrPhe: 2.021 ± 0.044
5.343ThrGly: 5.343 ± 0.104
0.935ThrHis: 0.935 ± 0.033
2.987ThrIle: 2.987 ± 0.06
1.512ThrLys: 1.512 ± 0.041
5.319ThrLeu: 5.319 ± 0.078
1.227ThrMet: 1.227 ± 0.04
1.487ThrAsn: 1.487 ± 0.052
3.149ThrPro: 3.149 ± 0.057
1.697ThrGln: 1.697 ± 0.049
3.326ThrArg: 3.326 ± 0.061
3.073ThrSer: 3.073 ± 0.063
2.571ThrThr: 2.571 ± 0.058
3.785ThrVal: 3.785 ± 0.066
0.608ThrTrp: 0.608 ± 0.028
1.258ThrTyr: 1.258 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
8.368ValAla: 8.368 ± 0.103
0.562ValCys: 0.562 ± 0.026
4.25ValAsp: 4.25 ± 0.074
4.899ValGlu: 4.899 ± 0.084
2.462ValPhe: 2.462 ± 0.053
5.397ValGly: 5.397 ± 0.076
1.201ValHis: 1.201 ± 0.041
3.919ValIle: 3.919 ± 0.067
1.955ValLys: 1.955 ± 0.051
6.674ValLeu: 6.674 ± 0.086
1.737ValMet: 1.737 ± 0.047
2.007ValAsn: 2.007 ± 0.052
3.64ValPro: 3.64 ± 0.057
1.985ValGln: 1.985 ± 0.047
4.459ValArg: 4.459 ± 0.07
4.292ValSer: 4.292 ± 0.075
4.252ValThr: 4.252 ± 0.076
5.138ValVal: 5.138 ± 0.073
0.879ValTrp: 0.879 ± 0.027
1.3ValTyr: 1.3 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.244TrpAla: 1.244 ± 0.041
0.116TrpCys: 0.116 ± 0.01
0.741TrpAsp: 0.741 ± 0.028
0.773TrpGlu: 0.773 ± 0.028
0.6TrpPhe: 0.6 ± 0.024
0.989TrpGly: 0.989 ± 0.035
0.33TrpHis: 0.33 ± 0.019
0.673TrpIle: 0.673 ± 0.027
0.451TrpLys: 0.451 ± 0.021
1.745TrpLeu: 1.745 ± 0.05
0.362TrpMet: 0.362 ± 0.02
0.43TrpAsn: 0.43 ± 0.018
0.645TrpPro: 0.645 ± 0.028
0.601TrpGln: 0.601 ± 0.022
1.161TrpArg: 1.161 ± 0.04
0.899TrpSer: 0.899 ± 0.031
0.744TrpThr: 0.744 ± 0.028
0.839TrpVal: 0.839 ± 0.033
0.235TrpTrp: 0.235 ± 0.016
0.29TrpTyr: 0.29 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.485TyrAla: 2.485 ± 0.053
0.244TyrCys: 0.244 ± 0.018
1.568TyrAsp: 1.568 ± 0.043
1.304TyrGlu: 1.304 ± 0.037
0.907TyrPhe: 0.907 ± 0.031
2.004TyrGly: 2.004 ± 0.047
0.464TyrHis: 0.464 ± 0.02
0.93TyrIle: 0.93 ± 0.035
0.582TyrLys: 0.582 ± 0.028
2.021TyrLeu: 2.021 ± 0.047
0.439TyrMet: 0.439 ± 0.023
0.598TyrAsn: 0.598 ± 0.026
1.019TyrPro: 1.019 ± 0.033
0.676TyrGln: 0.676 ± 0.028
1.721TyrArg: 1.721 ± 0.045
1.318TyrSer: 1.318 ± 0.037
1.081TyrThr: 1.081 ± 0.037
1.467TyrVal: 1.467 ± 0.036
0.367TyrTrp: 0.367 ± 0.022
0.594TyrTyr: 0.594 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3177 proteins (1002534 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski