Bacteroides finegoldii CL09T03C10
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K5BUP7|K5BUP7_9BACE Uncharacterized protein OS=Bacteroides finegoldii CL09T03C10 OX=997888 GN=HMPREF1057_00767 PE=4 SV=1
MM1 pKa = 6.39 THH3 pKa = 6.41 YY4 pKa = 10.49 EE5 pKa = 3.99 IEE7 pKa = 4.05 QGLNALYY14 pKa = 10.62 KK15 pKa = 10.59 DD16 pKa = 4.05 LNNVQDD22 pKa = 3.82 MDD24 pKa = 3.71 EE25 pKa = 4.05 ATARR29 pKa = 11.84 RR30 pKa = 11.84 VYY32 pKa = 10.93 NVDD35 pKa = 3.33 CKK37 pKa = 11.36 ADD39 pKa = 3.46 IIEE42 pKa = 4.52 VIEE45 pKa = 4.2 EE46 pKa = 4.33 EE47 pKa = 3.95 IDD49 pKa = 3.34 TYY51 pKa = 11.0 EE52 pKa = 5.27 AILSGPDD59 pKa = 3.15 TDD61 pKa = 4.96 EE62 pKa = 5.61 DD63 pKa = 4.16 NDD65 pKa = 3.42 MDD67 pKa = 4.8 YY68 pKa = 11.52 DD69 pKa = 4.23 ALCLIQGLSRR79 pKa = 11.84 YY80 pKa = 9.07 AA81 pKa = 3.39
Molecular weight: 9.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.719
IPC_protein 3.681
Toseland 3.478
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.808
Patrickios 0.54
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|K5CPS6|K5CPS6_9BACE Uncharacterized protein OS=Bacteroides finegoldii CL09T03C10 OX=997888 GN=HMPREF1057_00626 PE=4 SV=1
MM1 pKa = 7.67 RR2 pKa = 11.84 ALAANNQNVSRR13 pKa = 11.84 GGINRR18 pKa = 11.84 QGRR21 pKa = 11.84 ILRR24 pKa = 11.84 ANAGALLPIYY34 pKa = 9.86 QRR36 pKa = 11.84 QGNRR40 pKa = 11.84 AAVNAMRR47 pKa = 11.84 SRR49 pKa = 11.84 LGLTNGG55 pKa = 3.54
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.368
IPC2_protein 11.008
IPC_protein 12.398
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4159
0
4159
1522812
29
2521
366.1
41.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.864 ± 0.029
1.229 ± 0.013
5.545 ± 0.028
6.752 ± 0.04
4.547 ± 0.022
6.772 ± 0.038
1.799 ± 0.017
6.933 ± 0.036
6.884 ± 0.034
9.0 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.745 ± 0.015
5.193 ± 0.028
3.685 ± 0.019
3.463 ± 0.022
4.604 ± 0.025
6.118 ± 0.035
5.629 ± 0.03
6.374 ± 0.029
1.302 ± 0.014
4.562 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here