Inquilinus limosus MP06
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6228 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0DEN5|A0A0A0DEN5_9PROT Lon protease OS=Inquilinus limosus MP06 OX=1398085 GN=lon PE=2 SV=1
MM1 pKa = 7.31 NVNISIDD8 pKa = 3.44 IPISRR13 pKa = 11.84 IADD16 pKa = 3.86 LVCNCFEE23 pKa = 4.44 GSYY26 pKa = 10.55 SPWIGSANLAPGEE39 pKa = 4.02 HH40 pKa = 7.43 AIPGLVFWAQPLLYY54 pKa = 8.71 EE55 pKa = 4.14 QPRR58 pKa = 11.84 FGIVVTYY65 pKa = 10.01 DD66 pKa = 3.44 GPDD69 pKa = 3.44 DD70 pKa = 5.04 DD71 pKa = 5.09 EE72 pKa = 6.94 GSFASQKK79 pKa = 9.92 TITLEE84 pKa = 3.84 DD85 pKa = 3.45 LRR87 pKa = 11.84 KK88 pKa = 10.2 GLEE91 pKa = 4.0 LMAKK95 pKa = 9.62 EE96 pKa = 4.39 EE97 pKa = 4.63 PYY99 pKa = 10.9 QFGNFLSDD107 pKa = 3.32 NDD109 pKa = 4.23 DD110 pKa = 5.04 AITADD115 pKa = 3.19 TFMQLVVLGEE125 pKa = 4.11 VVYY128 pKa = 10.79 GG129 pKa = 3.79
Molecular weight: 14.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A0A0D1K5|A0A0A0D1K5_9PROT LacI family transcriptional regulator OS=Inquilinus limosus MP06 OX=1398085 GN=P409_24780 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 8.66 TAKK6 pKa = 10.02 AAPAEE11 pKa = 4.21 TSEE14 pKa = 4.04 GRR16 pKa = 11.84 CLCGRR21 pKa = 11.84 VRR23 pKa = 11.84 VEE25 pKa = 3.49 IGVPARR31 pKa = 11.84 WAWHH35 pKa = 4.99 DD36 pKa = 3.19 HH37 pKa = 4.96 SRR39 pKa = 11.84 NSRR42 pKa = 11.84 LAHH45 pKa = 5.42 GAAYY49 pKa = 7.77 ATYY52 pKa = 8.58 VGCWRR57 pKa = 11.84 SRR59 pKa = 11.84 VRR61 pKa = 11.84 IVKK64 pKa = 10.41 GEE66 pKa = 3.92 SALARR71 pKa = 11.84 FTEE74 pKa = 4.53 EE75 pKa = 3.35 EE76 pKa = 3.92 TGRR79 pKa = 11.84 IRR81 pKa = 11.84 SFCRR85 pKa = 11.84 HH86 pKa = 6.47 CGTPVLYY93 pKa = 9.74 EE94 pKa = 4.28 RR95 pKa = 11.84 PHH97 pKa = 6.36 APQMVNIPRR106 pKa = 11.84 ALFEE110 pKa = 4.29 TRR112 pKa = 11.84 TGRR115 pKa = 11.84 EE116 pKa = 3.79 PLYY119 pKa = 10.85 HH120 pKa = 7.65 IGIEE124 pKa = 4.52 QARR127 pKa = 11.84 EE128 pKa = 3.96 WTWRR132 pKa = 11.84 GEE134 pKa = 3.93 PLRR137 pKa = 11.84 PLKK140 pKa = 10.57 GFPGVVWTGPKK151 pKa = 9.46 RR152 pKa = 11.84 RR153 pKa = 11.84 KK154 pKa = 9.38 KK155 pKa = 10.6 RR156 pKa = 11.84 PLLDD160 pKa = 3.33 EE161 pKa = 4.62 AFF163 pKa = 4.01
Molecular weight: 18.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.487
IPC_protein 10.277
Toseland 10.511
ProMoST 10.233
Dawson 10.628
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.774
Grimsley 10.687
Solomon 10.745
Lehninger 10.701
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.482
IPC_peptide 10.745
IPC2_peptide 9.619
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6228
0
6228
1722682
26
1966
276.6
29.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.301 ± 0.041
0.796 ± 0.009
5.84 ± 0.028
5.268 ± 0.027
3.571 ± 0.017
9.208 ± 0.034
1.966 ± 0.016
4.88 ± 0.024
2.547 ± 0.026
10.609 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.014
2.14 ± 0.018
5.657 ± 0.021
3.047 ± 0.018
7.588 ± 0.033
4.707 ± 0.019
5.216 ± 0.021
7.715 ± 0.024
1.478 ± 0.015
2.206 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here