Peptococcus niger
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6SIS9|A0A1G6SIS9_PEPNI Thiamine diphosphokinase OS=Peptococcus niger OX=2741 GN=SAMN04489866_101329 PE=4 SV=1
MM1 pKa = 8.21 DD2 pKa = 5.16 KK3 pKa = 10.85 PPLHH7 pKa = 5.2 THH9 pKa = 6.34 YY10 pKa = 10.6 YY11 pKa = 9.42 ALDD14 pKa = 3.64 VAEE17 pKa = 4.99 DD18 pKa = 3.78 WQSFTYY24 pKa = 9.65 ICKK27 pKa = 10.18 EE28 pKa = 3.74 NVMDD32 pKa = 5.58 QMDD35 pKa = 4.64 RR36 pKa = 11.84 YY37 pKa = 10.93 NFAAANWAYY46 pKa = 9.38 TLEE49 pKa = 4.35 DD50 pKa = 3.84 LEE52 pKa = 4.79 EE53 pKa = 4.16 KK54 pKa = 10.07 TFSILLFITGGDD66 pKa = 3.67 EE67 pKa = 3.83 ATVAQMILDD76 pKa = 4.23 AEE78 pKa = 4.32 AEE80 pKa = 4.31 KK81 pKa = 11.22 NDD83 pKa = 3.53
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.806
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.77
ProMoST 4.037
Dawson 3.935
Bjellqvist 4.151
Wikipedia 3.859
Rodwell 3.795
Grimsley 3.681
Solomon 3.923
Lehninger 3.884
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.821
EMBOSS 3.872
Sillero 4.075
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.995
Protein with the highest isoelectric point:
>tr|A0A1G6Z919|A0A1G6Z919_PEPNI Pyruvate orthophosphate dikinase OS=Peptococcus niger OX=2741 GN=SAMN04489866_11230 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 9.01 QPKK8 pKa = 8.93 RR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 8.0 HH12 pKa = 4.33 QRR14 pKa = 11.84 VHH16 pKa = 6.02 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MLTKK25 pKa = 10.43 NGRR28 pKa = 11.84 NVLKK32 pKa = 10.49 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.451
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.179
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1844
0
1844
584678
30
3708
317.1
35.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.865 ± 0.09
1.133 ± 0.025
6.177 ± 0.054
6.248 ± 0.06
3.837 ± 0.046
7.465 ± 0.059
2.005 ± 0.025
6.269 ± 0.065
5.59 ± 0.083
10.139 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.035
3.621 ± 0.045
4.233 ± 0.042
3.636 ± 0.036
5.204 ± 0.054
5.216 ± 0.04
5.38 ± 0.059
6.901 ± 0.046
0.977 ± 0.018
3.413 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here