Amino acid dipepetide frequency for Peptococcus niger

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.431AlaAla: 10.431 ± 0.225
1.216AlaCys: 1.216 ± 0.048
6.617AlaAsp: 6.617 ± 0.122
6.518AlaGlu: 6.518 ± 0.135
3.936AlaPhe: 3.936 ± 0.08
7.56AlaGly: 7.56 ± 0.142
2.09AlaHis: 2.09 ± 0.06
5.803AlaIle: 5.803 ± 0.108
5.164AlaLys: 5.164 ± 0.112
11.488AlaLeu: 11.488 ± 0.202
2.617AlaMet: 2.617 ± 0.08
3.029AlaAsn: 3.029 ± 0.08
3.773AlaPro: 3.773 ± 0.084
3.756AlaGln: 3.756 ± 0.087
4.512AlaArg: 4.512 ± 0.096
4.466AlaSer: 4.466 ± 0.091
4.351AlaThr: 4.351 ± 0.093
7.136AlaVal: 7.136 ± 0.111
0.874AlaTrp: 0.874 ± 0.039
3.31AlaTyr: 3.31 ± 0.08
0.0AlaXaa: 0.0 ± 0.0
Cys
1.028CysAla: 1.028 ± 0.052
0.181CysCys: 0.181 ± 0.016
0.681CysAsp: 0.681 ± 0.033
0.604CysGlu: 0.604 ± 0.034
0.39CysPhe: 0.39 ± 0.026
1.243CysGly: 1.243 ± 0.05
0.322CysHis: 0.322 ± 0.027
0.676CysIle: 0.676 ± 0.035
0.405CysLys: 0.405 ± 0.026
1.144CysLeu: 1.144 ± 0.052
0.25CysMet: 0.25 ± 0.021
0.369CysAsn: 0.369 ± 0.025
0.648CysPro: 0.648 ± 0.037
0.388CysGln: 0.388 ± 0.03
0.744CysArg: 0.744 ± 0.035
0.578CysSer: 0.578 ± 0.033
0.53CysThr: 0.53 ± 0.03
0.658CysVal: 0.658 ± 0.037
0.108CysTrp: 0.108 ± 0.013
0.383CysTyr: 0.383 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.514AspAla: 5.514 ± 0.112
0.712AspCys: 0.712 ± 0.037
3.498AspAsp: 3.498 ± 0.087
4.072AspGlu: 4.072 ± 0.084
2.577AspPhe: 2.577 ± 0.067
4.928AspGly: 4.928 ± 0.116
1.363AspHis: 1.363 ± 0.047
4.062AspIle: 4.062 ± 0.083
3.968AspLys: 3.968 ± 0.154
6.729AspLeu: 6.729 ± 0.112
1.692AspMet: 1.692 ± 0.054
1.989AspAsn: 1.989 ± 0.066
2.509AspPro: 2.509 ± 0.072
2.288AspGln: 2.288 ± 0.068
3.501AspArg: 3.501 ± 0.083
2.362AspSer: 2.362 ± 0.065
2.749AspThr: 2.749 ± 0.086
4.24AspVal: 4.24 ± 0.105
0.729AspTrp: 0.729 ± 0.037
2.292AspTyr: 2.292 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
7.351GluAla: 7.351 ± 0.135
0.469GluCys: 0.469 ± 0.03
3.855GluAsp: 3.855 ± 0.087
4.821GluGlu: 4.821 ± 0.118
1.71GluPhe: 1.71 ± 0.059
4.416GluGly: 4.416 ± 0.092
1.016GluHis: 1.016 ± 0.047
3.66GluIle: 3.66 ± 0.078
4.922GluLys: 4.922 ± 0.119
5.312GluLeu: 5.312 ± 0.124
1.845GluMet: 1.845 ± 0.061
2.673GluAsn: 2.673 ± 0.077
1.993GluPro: 1.993 ± 0.062
2.193GluGln: 2.193 ± 0.071
3.634GluArg: 3.634 ± 0.094
2.714GluSer: 2.714 ± 0.066
3.282GluThr: 3.282 ± 0.081
4.401GluVal: 4.401 ± 0.093
0.537GluTrp: 0.537 ± 0.03
1.678GluTyr: 1.678 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
3.4PheAla: 3.4 ± 0.087
0.518PheCys: 0.518 ± 0.03
2.471PheAsp: 2.471 ± 0.059
1.993PheGlu: 1.993 ± 0.062
1.806PhePhe: 1.806 ± 0.067
2.803PheGly: 2.803 ± 0.087
0.722PheHis: 0.722 ± 0.031
2.856PheIle: 2.856 ± 0.073
2.054PheLys: 2.054 ± 0.06
3.727PheLeu: 3.727 ± 0.104
1.067PheMet: 1.067 ± 0.048
1.56PheAsn: 1.56 ± 0.057
1.428PhePro: 1.428 ± 0.05
1.231PheGln: 1.231 ± 0.045
1.58PheArg: 1.58 ± 0.054
2.658PheSer: 2.658 ± 0.078
2.145PheThr: 2.145 ± 0.068
2.359PheVal: 2.359 ± 0.067
0.441PheTrp: 0.441 ± 0.031
1.546PheTyr: 1.546 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
6.294GlyAla: 6.294 ± 0.128
0.97GlyCys: 0.97 ± 0.042
4.308GlyAsp: 4.308 ± 0.093
4.271GlyGlu: 4.271 ± 0.081
2.993GlyPhe: 2.993 ± 0.072
5.545GlyGly: 5.545 ± 0.125
1.792GlyHis: 1.792 ± 0.055
5.085GlyIle: 5.085 ± 0.115
4.727GlyLys: 4.727 ± 0.114
7.996GlyLeu: 7.996 ± 0.16
2.22GlyMet: 2.22 ± 0.069
2.475GlyAsn: 2.475 ± 0.074
2.263GlyPro: 2.263 ± 0.062
3.039GlyGln: 3.039 ± 0.084
3.881GlyArg: 3.881 ± 0.096
3.987GlySer: 3.987 ± 0.086
3.968GlyThr: 3.968 ± 0.108
4.972GlyVal: 4.972 ± 0.11
0.73GlyTrp: 0.73 ± 0.05
3.133GlyTyr: 3.133 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
1.517HisAla: 1.517 ± 0.053
0.267HisCys: 0.267 ± 0.023
0.973HisAsp: 0.973 ± 0.04
1.076HisGlu: 1.076 ± 0.052
0.949HisPhe: 0.949 ± 0.041
1.382HisGly: 1.382 ± 0.046
0.578HisHis: 0.578 ± 0.033
1.355HisIle: 1.355 ± 0.051
0.939HisLys: 0.939 ± 0.043
2.35HisLeu: 2.35 ± 0.071
0.595HisMet: 0.595 ± 0.03
0.633HisAsn: 0.633 ± 0.036
1.098HisPro: 1.098 ± 0.047
0.833HisGln: 0.833 ± 0.041
1.206HisArg: 1.206 ± 0.052
0.992HisSer: 0.992 ± 0.036
0.994HisThr: 0.994 ± 0.04
1.182HisVal: 1.182 ± 0.052
0.286HisTrp: 0.286 ± 0.026
0.845HisTyr: 0.845 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.993IleAla: 5.993 ± 0.12
0.93IleCys: 0.93 ± 0.04
4.16IleAsp: 4.16 ± 0.095
3.58IleGlu: 3.58 ± 0.088
2.687IlePhe: 2.687 ± 0.077
5.278IleGly: 5.278 ± 0.116
1.105IleHis: 1.105 ± 0.043
4.177IleIle: 4.177 ± 0.107
3.039IleLys: 3.039 ± 0.072
5.985IleLeu: 5.985 ± 0.119
1.493IleMet: 1.493 ± 0.056
2.523IleAsn: 2.523 ± 0.073
2.771IlePro: 2.771 ± 0.074
1.883IleGln: 1.883 ± 0.052
2.964IleArg: 2.964 ± 0.076
3.941IleSer: 3.941 ± 0.086
3.561IleThr: 3.561 ± 0.09
4.04IleVal: 4.04 ± 0.085
0.515IleTrp: 0.515 ± 0.029
2.066IleTyr: 2.066 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.68LysAla: 5.68 ± 0.122
0.366LysCys: 0.366 ± 0.027
4.048LysAsp: 4.048 ± 0.155
4.425LysGlu: 4.425 ± 0.104
1.416LysPhe: 1.416 ± 0.056
4.023LysGly: 4.023 ± 0.11
0.818LysHis: 0.818 ± 0.041
3.539LysIle: 3.539 ± 0.083
3.997LysLys: 3.997 ± 0.105
4.189LysLeu: 4.189 ± 0.089
1.469LysMet: 1.469 ± 0.058
2.745LysAsn: 2.745 ± 0.084
2.372LysPro: 2.372 ± 0.103
1.758LysGln: 1.758 ± 0.058
3.014LysArg: 3.014 ± 0.074
2.872LysSer: 2.872 ± 0.074
3.383LysThr: 3.383 ± 0.102
3.563LysVal: 3.563 ± 0.111
0.535LysTrp: 0.535 ± 0.033
1.683LysTyr: 1.683 ± 0.064
0.002LysXaa: 0.002 ± 0.002
Leu
11.562LeuAla: 11.562 ± 0.206
1.19LeuCys: 1.19 ± 0.05
5.772LeuAsp: 5.772 ± 0.116
5.716LeuGlu: 5.716 ± 0.109
3.636LeuPhe: 3.636 ± 0.096
6.51LeuGly: 6.51 ± 0.138
1.671LeuHis: 1.671 ± 0.054
5.87LeuIle: 5.87 ± 0.116
5.542LeuLys: 5.542 ± 0.097
10.204LeuLeu: 10.204 ± 0.197
2.796LeuMet: 2.796 ± 0.078
3.641LeuAsn: 3.641 ± 0.082
5.121LeuPro: 5.121 ± 0.104
3.467LeuGln: 3.467 ± 0.085
4.999LeuArg: 4.999 ± 0.127
6.576LeuSer: 6.576 ± 0.116
6.472LeuThr: 6.472 ± 0.12
6.706LeuVal: 6.706 ± 0.138
0.852LeuTrp: 0.852 ± 0.036
3.085LeuTyr: 3.085 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
3.12MetAla: 3.12 ± 0.078
0.204MetCys: 0.204 ± 0.019
1.657MetAsp: 1.657 ± 0.047
1.507MetGlu: 1.507 ± 0.057
0.694MetPhe: 0.694 ± 0.04
1.943MetGly: 1.943 ± 0.06
0.469MetHis: 0.469 ± 0.03
1.57MetIle: 1.57 ± 0.06
1.591MetLys: 1.591 ± 0.057
2.394MetLeu: 2.394 ± 0.076
0.749MetMet: 0.749 ± 0.039
1.129MetAsn: 1.129 ± 0.047
1.271MetPro: 1.271 ± 0.05
1.006MetGln: 1.006 ± 0.046
1.467MetArg: 1.467 ± 0.051
1.587MetSer: 1.587 ± 0.057
1.9MetThr: 1.9 ± 0.063
1.94MetVal: 1.94 ± 0.066
0.159MetTrp: 0.159 ± 0.016
0.561MetTyr: 0.561 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.036AsnAla: 3.036 ± 0.065
0.452AsnCys: 0.452 ± 0.031
2.085AsnAsp: 2.085 ± 0.073
2.114AsnGlu: 2.114 ± 0.064
1.334AsnPhe: 1.334 ± 0.051
2.784AsnGly: 2.784 ± 0.087
0.8AsnHis: 0.8 ± 0.038
2.6AsnIle: 2.6 ± 0.08
2.22AsnLys: 2.22 ± 0.08
3.375AsnLeu: 3.375 ± 0.081
1.036AsnMet: 1.036 ± 0.046
1.435AsnAsn: 1.435 ± 0.059
2.066AsnPro: 2.066 ± 0.075
1.384AsnGln: 1.384 ± 0.06
2.241AsnArg: 2.241 ± 0.058
1.509AsnSer: 1.509 ± 0.048
1.875AsnThr: 1.875 ± 0.058
2.133AsnVal: 2.133 ± 0.065
0.426AsnTrp: 0.426 ± 0.027
1.305AsnTyr: 1.305 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
4.37ProAla: 4.37 ± 0.102
0.405ProCys: 0.405 ± 0.025
3.003ProAsp: 3.003 ± 0.075
3.422ProGlu: 3.422 ± 0.077
1.815ProPhe: 1.815 ± 0.063
3.104ProGly: 3.104 ± 0.07
0.865ProHis: 0.865 ± 0.038
2.335ProIle: 2.335 ± 0.056
2.008ProLys: 2.008 ± 0.065
4.13ProLeu: 4.13 ± 0.099
1.009ProMet: 1.009 ± 0.046
1.414ProAsn: 1.414 ± 0.058
1.375ProPro: 1.375 ± 0.061
1.652ProGln: 1.652 ± 0.055
1.582ProArg: 1.582 ± 0.054
2.193ProSer: 2.193 ± 0.061
2.242ProThr: 2.242 ± 0.072
3.422ProVal: 3.422 ± 0.087
0.426ProTrp: 0.426 ± 0.026
1.553ProTyr: 1.553 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
4.527GlnAla: 4.527 ± 0.094
0.275GlnCys: 0.275 ± 0.019
2.047GlnAsp: 2.047 ± 0.069
2.557GlnGlu: 2.557 ± 0.073
1.165GlnPhe: 1.165 ± 0.041
2.61GlnGly: 2.61 ± 0.068
0.57GlnHis: 0.57 ± 0.033
2.063GlnIle: 2.063 ± 0.065
2.155GlnLys: 2.155 ± 0.066
3.551GlnLeu: 3.551 ± 0.095
1.084GlnMet: 1.084 ± 0.041
1.225GlnAsn: 1.225 ± 0.045
1.42GlnPro: 1.42 ± 0.055
1.252GlnGln: 1.252 ± 0.053
1.662GlnArg: 1.662 ± 0.061
1.75GlnSer: 1.75 ± 0.055
1.933GlnThr: 1.933 ± 0.066
3.06GlnVal: 3.06 ± 0.075
0.424GlnTrp: 0.424 ± 0.025
1.028GlnTyr: 1.028 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
4.505ArgAla: 4.505 ± 0.083
0.503ArgCys: 0.503 ± 0.033
2.836ArgAsp: 2.836 ± 0.076
3.251ArgGlu: 3.251 ± 0.09
2.181ArgPhe: 2.181 ± 0.065
2.986ArgGly: 2.986 ± 0.082
1.401ArgHis: 1.401 ± 0.056
3.315ArgIle: 3.315 ± 0.071
2.631ArgLys: 2.631 ± 0.083
6.304ArgLeu: 6.304 ± 0.155
1.464ArgMet: 1.464 ± 0.056
1.611ArgAsn: 1.611 ± 0.049
2.312ArgPro: 2.312 ± 0.072
2.518ArgGln: 2.518 ± 0.083
3.511ArgArg: 3.511 ± 0.106
2.444ArgSer: 2.444 ± 0.064
2.386ArgThr: 2.386 ± 0.066
3.234ArgVal: 3.234 ± 0.077
0.576ArgTrp: 0.576 ± 0.032
2.073ArgTyr: 2.073 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
4.604SerAla: 4.604 ± 0.096
0.626SerCys: 0.626 ± 0.041
3.2SerAsp: 3.2 ± 0.08
3.087SerGlu: 3.087 ± 0.08
2.314SerPhe: 2.314 ± 0.071
4.741SerGly: 4.741 ± 0.103
1.113SerHis: 1.113 ± 0.048
3.195SerIle: 3.195 ± 0.074
2.603SerLys: 2.603 ± 0.081
5.477SerLeu: 5.477 ± 0.09
1.444SerMet: 1.444 ± 0.052
1.681SerAsn: 1.681 ± 0.067
2.329SerPro: 2.329 ± 0.069
1.704SerGln: 1.704 ± 0.066
2.711SerArg: 2.711 ± 0.066
2.779SerSer: 2.779 ± 0.08
2.629SerThr: 2.629 ± 0.073
3.525SerVal: 3.525 ± 0.076
0.575SerTrp: 0.575 ± 0.03
1.823SerTyr: 1.823 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
5.531ThrAla: 5.531 ± 0.113
0.684ThrCys: 0.684 ± 0.038
3.44ThrAsp: 3.44 ± 0.093
3.108ThrGlu: 3.108 ± 0.078
2.249ThrPhe: 2.249 ± 0.079
4.683ThrGly: 4.683 ± 0.097
0.995ThrHis: 0.995 ± 0.04
3.381ThrIle: 3.381 ± 0.081
2.261ThrLys: 2.261 ± 0.083
5.552ThrLeu: 5.552 ± 0.1
1.243ThrMet: 1.243 ± 0.053
1.746ThrAsn: 1.746 ± 0.069
2.583ThrPro: 2.583 ± 0.067
1.551ThrGln: 1.551 ± 0.062
2.348ThrArg: 2.348 ± 0.059
2.504ThrSer: 2.504 ± 0.078
2.742ThrThr: 2.742 ± 0.089
4.792ThrVal: 4.792 ± 0.109
0.491ThrTrp: 0.491 ± 0.029
1.912ThrTyr: 1.912 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
6.426ValAla: 6.426 ± 0.107
0.852ValCys: 0.852 ± 0.042
4.269ValAsp: 4.269 ± 0.088
3.806ValGlu: 3.806 ± 0.093
2.719ValPhe: 2.719 ± 0.081
4.857ValGly: 4.857 ± 0.113
1.351ValHis: 1.351 ± 0.045
4.691ValIle: 4.691 ± 0.085
3.525ValLys: 3.525 ± 0.105
6.882ValLeu: 6.882 ± 0.133
1.738ValMet: 1.738 ± 0.056
2.75ValAsn: 2.75 ± 0.072
3.072ValPro: 3.072 ± 0.075
2.446ValGln: 2.446 ± 0.057
3.645ValArg: 3.645 ± 0.082
3.949ValSer: 3.949 ± 0.098
4.242ValThr: 4.242 ± 0.116
5.004ValVal: 5.004 ± 0.114
0.611ValTrp: 0.611 ± 0.033
2.174ValTyr: 2.174 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.824TrpAla: 0.824 ± 0.043
0.118TrpCys: 0.118 ± 0.015
0.568TrpAsp: 0.568 ± 0.034
0.503TrpGlu: 0.503 ± 0.033
0.359TrpPhe: 0.359 ± 0.028
0.624TrpGly: 0.624 ± 0.029
0.25TrpHis: 0.25 ± 0.02
0.523TrpIle: 0.523 ± 0.033
0.462TrpLys: 0.462 ± 0.029
1.141TrpLeu: 1.141 ± 0.051
0.27TrpMet: 0.27 ± 0.023
0.303TrpAsn: 0.303 ± 0.025
0.431TrpPro: 0.431 ± 0.03
0.749TrpGln: 0.749 ± 0.037
0.66TrpArg: 0.66 ± 0.035
0.51TrpSer: 0.51 ± 0.028
0.498TrpThr: 0.498 ± 0.035
0.595TrpVal: 0.595 ± 0.031
0.086TrpTrp: 0.086 ± 0.013
0.296TrpTyr: 0.296 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.074
0.393TyrCys: 0.393 ± 0.027
2.283TyrAsp: 2.283 ± 0.077
2.054TyrGlu: 2.054 ± 0.065
1.435TyrPhe: 1.435 ± 0.059
2.631TyrGly: 2.631 ± 0.069
0.758TyrHis: 0.758 ± 0.036
1.934TyrIle: 1.934 ± 0.059
1.683TyrLys: 1.683 ± 0.07
3.46TyrLeu: 3.46 ± 0.082
0.833TyrMet: 0.833 ± 0.039
1.276TyrAsn: 1.276 ± 0.051
1.493TyrPro: 1.493 ± 0.055
1.259TyrGln: 1.259 ± 0.042
2.179TyrArg: 2.179 ± 0.058
1.791TyrSer: 1.791 ± 0.063
1.917TyrThr: 1.917 ± 0.056
2.047TyrVal: 2.047 ± 0.078
0.39TyrTrp: 0.39 ± 0.024
1.385TyrTyr: 1.385 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.002
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.005XaaXaa: 0.005 ± 0.005
Statistics based on 1844 proteins (584678 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski