bacterium 1XD42-54

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9F9Q8|A0A3A9F9Q8_9BACT GntR family transcriptional regulator OS=bacterium 1XD42-54 OX=2320110 GN=D7Y05_12520 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.24KK3 pKa = 10.53AIVSVLLATVLLTSGMSVMADD24 pKa = 2.93EE25 pKa = 5.63GDD27 pKa = 3.87TIKK30 pKa = 10.52IGALFNLTGGQASIDD45 pKa = 3.76TPSYY49 pKa = 10.88QGFKK53 pKa = 10.79LKK55 pKa = 10.6ADD57 pKa = 4.81EE58 pKa = 4.32INAAGGINGRR68 pKa = 11.84MIEE71 pKa = 4.19VVSYY75 pKa = 10.8DD76 pKa = 3.4GKK78 pKa = 9.48TDD80 pKa = 3.72QATCANNAKK89 pKa = 10.39KK90 pKa = 10.17MIDD93 pKa = 3.3VDD95 pKa = 3.92DD96 pKa = 4.51CVVVSGFSDD105 pKa = 3.42SNYY108 pKa = 10.7ALAAGAITQDD118 pKa = 3.08KK119 pKa = 9.77QVPFVITGATLPDD132 pKa = 3.65LCEE135 pKa = 3.98QIGDD139 pKa = 3.81YY140 pKa = 11.24SFMVPFGDD148 pKa = 4.22DD149 pKa = 3.0TCAYY153 pKa = 9.94AAAEE157 pKa = 3.96YY158 pKa = 9.97AYY160 pKa = 10.64AEE162 pKa = 4.64IGTKK166 pKa = 9.89CYY168 pKa = 10.66MMIDD172 pKa = 3.28QSMEE176 pKa = 3.88FTKK179 pKa = 10.5TLAEE183 pKa = 4.28CFQEE187 pKa = 4.41RR188 pKa = 11.84FEE190 pKa = 4.23EE191 pKa = 4.32LGGEE195 pKa = 4.17IVGTDD200 pKa = 2.94NYY202 pKa = 10.79MNGDD206 pKa = 4.06PDD208 pKa = 4.82FSAQIDD214 pKa = 3.78KK215 pKa = 10.91FINNDD220 pKa = 2.87GGAEE224 pKa = 4.02FMFFSSVPNDD234 pKa = 3.51AGPLVLQYY242 pKa = 10.39RR243 pKa = 11.84DD244 pKa = 3.74KK245 pKa = 11.28GVEE248 pKa = 3.92LPIVSGDD255 pKa = 3.67GFDD258 pKa = 3.65TPLVAEE264 pKa = 4.32VAGAQSYY271 pKa = 11.13DD272 pKa = 3.58VFVSTHH278 pKa = 5.79CSYY281 pKa = 11.3TNEE284 pKa = 4.12AEE286 pKa = 4.12NVQNFVKK293 pKa = 10.47AYY295 pKa = 7.64TDD297 pKa = 3.34AYY299 pKa = 8.94GTAPEE304 pKa = 4.17NAFAALGYY312 pKa = 10.69DD313 pKa = 3.35AMDD316 pKa = 5.75LIGQALATVEE326 pKa = 5.06DD327 pKa = 4.33PTDD330 pKa = 3.07TAAIKK335 pKa = 10.4DD336 pKa = 4.15AIEE339 pKa = 4.14ATSDD343 pKa = 3.53FKK345 pKa = 11.54GVTGTISYY353 pKa = 9.97SAEE356 pKa = 3.56SHH358 pKa = 6.71KK359 pKa = 10.35PDD361 pKa = 3.08KK362 pKa = 10.03TASILEE368 pKa = 4.21LVDD371 pKa = 5.06GEE373 pKa = 4.49FQFVTEE379 pKa = 4.29EE380 pKa = 3.87

Molecular weight: 40.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9FCM9|A0A3A9FCM9_9BACT Dephospho-CoA kinase OS=bacterium 1XD42-54 OX=2320110 GN=coaE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3209

0

3209

1081669

17

7285

337.1

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.824 ± 0.043

1.533 ± 0.021

5.406 ± 0.038

7.896 ± 0.051

4.046 ± 0.034

7.304 ± 0.05

1.719 ± 0.016

6.821 ± 0.039

6.176 ± 0.043

9.167 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.065 ± 0.026

4.009 ± 0.029

3.274 ± 0.026

3.619 ± 0.029

4.816 ± 0.041

5.769 ± 0.035

5.489 ± 0.055

6.951 ± 0.035

0.957 ± 0.012

4.16 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski