Trichobilharzia regenti (Nasal bird schistosome)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P8JUP6|A0A3P8JUP6_TRIRE Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS18309 PE=4 SV=1
MM1 pKa = 8.06 DD2 pKa = 4.63 EE3 pKa = 4.42 SSSVSGTASVLGEE16 pKa = 4.14 TQLTSTLLYY25 pKa = 10.66 DD26 pKa = 4.15 VNPLTNSNNDD36 pKa = 3.16 NNNNDD41 pKa = 3.89 NNHH44 pKa = 6.23 HH45 pKa = 6.69 FSLTCSDD52 pKa = 3.68 STEE55 pKa = 4.23 SPLLNSTNIITTSSDD70 pKa = 3.26 QQQSLSVMCTVNQNDD85 pKa = 3.73 LSNNSGDD92 pKa = 3.56 GGGGGEE98 pKa = 3.85 QSIFDD103 pKa = 4.11 TVTVLNSTPSAIHH116 pKa = 6.35 SEE118 pKa = 4.23 VLQEE122 pKa = 4.08 STISLEE128 pKa = 4.16 LTTSDD133 pKa = 4.32 SNDD136 pKa = 2.9 ASFSSLNSEE145 pKa = 5.62 RR146 pKa = 11.84 IDD148 pKa = 3.95 CLKK151 pKa = 10.78 EE152 pKa = 3.51 YY153 pKa = 11.17 LNFNKK158 pKa = 9.86 TPATEE163 pKa = 4.18 TLNNNSIVYY172 pKa = 7.93 TLKK175 pKa = 11.02 DD176 pKa = 3.43 SLHH179 pKa = 6.63 SNNNNNNDD187 pKa = 3.51 NTNNTDD193 pKa = 3.79 LNNSPLTNDD202 pKa = 3.58 LSDD205 pKa = 3.8 LTSHH209 pKa = 6.33 VNNSSTSSATTTVALTSPICQLPTSVV235 pKa = 4.0
Molecular weight: 25.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A3P8INS7|A0A3P8INS7_TRIRE Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS2059 PE=4 SV=1
MM1 pKa = 7.14 MSRR4 pKa = 11.84 RR5 pKa = 11.84 TLLKK9 pKa = 10.05 VILTVRR15 pKa = 11.84 PQIVPMLNLRR25 pKa = 11.84 RR26 pKa = 11.84 NRR28 pKa = 11.84 NRR30 pKa = 11.84 SS31 pKa = 3.1
Molecular weight: 3.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22165
0
22165
4891284
29
3889
220.7
24.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.948 ± 0.017
2.138 ± 0.012
5.177 ± 0.019
5.434 ± 0.02
3.74 ± 0.014
4.529 ± 0.02
2.826 ± 0.011
5.897 ± 0.015
5.415 ± 0.022
9.249 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.008
6.685 ± 0.026
4.771 ± 0.019
4.298 ± 0.017
4.917 ± 0.016
10.507 ± 0.035
6.457 ± 0.026
5.531 ± 0.015
0.924 ± 0.005
3.199 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here