Microbacterium protaetiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6ES60|A0A4P6ES60_9MICO Thioredoxin OS=Microbacterium protaetiae OX=2509458 GN=ET475_12865 PE=4 SV=1
MM1 pKa = 7.14STTTYY6 pKa = 10.07RR7 pKa = 11.84VNGMTCAHH15 pKa = 6.99CVQAVTRR22 pKa = 11.84EE23 pKa = 3.92VSALDD28 pKa = 3.84GVDD31 pKa = 3.64DD32 pKa = 5.31AVVDD36 pKa = 4.58LQAGTVAVTGAASEE50 pKa = 4.32TDD52 pKa = 3.3VAAAVAEE59 pKa = 4.25AGYY62 pKa = 10.88ALAGTASS69 pKa = 3.31

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6EDQ1|A0A4P6EDQ1_9MICO D-inositol 3-phosphate glycosyltransferase OS=Microbacterium protaetiae OX=2509458 GN=ET475_10610 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3400

0

3400

1112479

32

2834

327.2

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.798 ± 0.073

0.526 ± 0.01

6.425 ± 0.044

5.233 ± 0.042

3.091 ± 0.027

8.768 ± 0.037

2.127 ± 0.02

4.569 ± 0.032

2.016 ± 0.031

9.896 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.02

1.9 ± 0.022

5.323 ± 0.029

2.994 ± 0.022

7.433 ± 0.056

5.414 ± 0.035

6.179 ± 0.043

8.944 ± 0.041

1.539 ± 0.02

1.981 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski