Eimeria brunetti RNA virus 1
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q98XW9|Q98XW9_9VIRU Coat protein OS=Eimeria brunetti RNA virus 1 OX=155414 PE=4 SV=1
MM1 pKa = 8.05 SIGTSNALRR10 pKa = 11.84 TRR12 pKa = 11.84 TNCGRR17 pKa = 11.84 KK18 pKa = 8.47 MDD20 pKa = 3.8 SGYY23 pKa = 10.8 KK24 pKa = 9.92 ISCLLGEE31 pKa = 4.54 VVPGGARR38 pKa = 11.84 VEE40 pKa = 4.29 VPTFGNYY47 pKa = 8.09 RR48 pKa = 11.84 KK49 pKa = 10.24 YY50 pKa = 10.04 MSRR53 pKa = 11.84 LEE55 pKa = 3.93 MRR57 pKa = 11.84 ACTRR61 pKa = 11.84 GLPQSTYY68 pKa = 10.81 CPIQFEE74 pKa = 4.25 VGAAFNLGTCAKK86 pKa = 9.58 NFKK89 pKa = 10.52 RR90 pKa = 11.84 EE91 pKa = 3.43 TWLQHH96 pKa = 5.02 VMRR99 pKa = 11.84 EE100 pKa = 4.32 GPMLGCSYY108 pKa = 8.97 ATPVEE113 pKa = 4.06 VRR115 pKa = 11.84 KK116 pKa = 9.98 VLKK119 pKa = 10.02 EE120 pKa = 3.56 EE121 pKa = 4.18 RR122 pKa = 11.84 FRR124 pKa = 11.84 QVGQAEE130 pKa = 4.25 WLTFNFDD137 pKa = 4.7 AIVCVLGAVAATLTLVPDD155 pKa = 4.74 LSTADD160 pKa = 3.34 WRR162 pKa = 11.84 NTSPSVRR169 pKa = 11.84 GLVSVEE175 pKa = 4.34 DD176 pKa = 3.29 LHH178 pKa = 7.12 AANSGGVVYY187 pKa = 10.22 YY188 pKa = 9.75 DD189 pKa = 3.54 PCALGIHH196 pKa = 6.4 AEE198 pKa = 4.15 CAKK201 pKa = 10.32 WLMIYY206 pKa = 9.87 AANVCGATVVVPHH219 pKa = 6.55 LTLDD223 pKa = 3.49 VNNRR227 pKa = 11.84 PICKK231 pKa = 9.97 DD232 pKa = 3.09 YY233 pKa = 11.57 DD234 pKa = 3.65 GAEE237 pKa = 4.11 LRR239 pKa = 11.84 RR240 pKa = 11.84 GLSVLLNILGTYY252 pKa = 10.56 YY253 pKa = 11.57 SMANAGDD260 pKa = 4.06 MYY262 pKa = 11.44 ALALMTGVHH271 pKa = 6.12 RR272 pKa = 11.84 VLTVVGHH279 pKa = 5.78 SDD281 pKa = 3.06 EE282 pKa = 5.24 GGWLRR287 pKa = 11.84 DD288 pKa = 3.62 VLRR291 pKa = 11.84 QGWWQQPHH299 pKa = 6.06 GGVPRR304 pKa = 11.84 SLPFSSGISVDD315 pKa = 3.66 DD316 pKa = 3.87 GRR318 pKa = 11.84 TLGTFVALVDD328 pKa = 4.75 SIALGTAAAVAHH340 pKa = 6.99 CDD342 pKa = 3.4 PLVPVGDD349 pKa = 3.73 NVFPTVFTVDD359 pKa = 3.65 LPEE362 pKa = 6.54 DD363 pKa = 4.0 GEE365 pKa = 4.35 LANGVMQRR373 pKa = 11.84 LMARR377 pKa = 11.84 TIADD381 pKa = 3.59 GCAKK385 pKa = 10.4 FSEE388 pKa = 5.06 LYY390 pKa = 9.43 ATALAKK396 pKa = 10.51 LFRR399 pKa = 11.84 LHH401 pKa = 6.96 VNVTEE406 pKa = 3.98 LHH408 pKa = 5.91 AAGVLQQMASYY419 pKa = 9.4 FAEE422 pKa = 4.28 SGSRR426 pKa = 11.84 HH427 pKa = 5.16 TDD429 pKa = 2.93 MAVCHH434 pKa = 6.0 PFFWIEE440 pKa = 3.85 PTTVLPAGEE449 pKa = 4.5 FGFPAEE455 pKa = 4.04 IYY457 pKa = 10.41 GYY459 pKa = 11.29 ASLCHH464 pKa = 5.33 ATQRR468 pKa = 11.84 RR469 pKa = 11.84 TMPFFEE475 pKa = 6.31 AIEE478 pKa = 4.13 EE479 pKa = 4.21 EE480 pKa = 4.54 GLSDD484 pKa = 3.34 TVVSHH489 pKa = 4.63 WRR491 pKa = 11.84 VRR493 pKa = 11.84 IRR495 pKa = 11.84 SPRR498 pKa = 11.84 TCGYY502 pKa = 10.57 VLYY505 pKa = 7.92 TTNHH509 pKa = 6.53 PKK511 pKa = 10.65 NGLANLLLRR520 pKa = 11.84 GFNSSALVLGTKK532 pKa = 10.03 SPEE535 pKa = 3.8 EE536 pKa = 3.91 IRR538 pKa = 11.84 CKK540 pKa = 10.15 KK541 pKa = 8.76 AAKK544 pKa = 10.29 GFVTFADD551 pKa = 4.15 LMWKK555 pKa = 10.21 RR556 pKa = 11.84 GQAKK560 pKa = 9.66 LPHH563 pKa = 6.46 PAEE566 pKa = 4.2 VLNVDD571 pKa = 3.39 GTFTVSILHH580 pKa = 6.05 AAITTRR586 pKa = 11.84 HH587 pKa = 4.19 YY588 pKa = 10.68 QKK590 pKa = 10.07 PIEE593 pKa = 5.06 HH594 pKa = 6.94 IPPPEE599 pKa = 4.47 DD600 pKa = 3.28 LSRR603 pKa = 11.84 MYY605 pKa = 9.99 LTLHH609 pKa = 6.33 ATRR612 pKa = 11.84 LAYY615 pKa = 10.47 YY616 pKa = 10.07 KK617 pKa = 10.55 AGPANVEE624 pKa = 3.86 PGGVRR629 pKa = 11.84 RR630 pKa = 11.84 LRR632 pKa = 11.84 NMGVDD637 pKa = 5.28 AINDD641 pKa = 3.63 MNGVVQNTMLRR652 pKa = 11.84 MSSFPPPSEE661 pKa = 3.85 TAPVLNKK668 pKa = 9.81 PGRR671 pKa = 11.84 AFGGAAAAVSEE682 pKa = 4.48 RR683 pKa = 11.84 CVEE686 pKa = 4.08 PVRR689 pKa = 11.84 GDD691 pKa = 3.33 AAEE694 pKa = 4.14 EE695 pKa = 4.21 SPPRR699 pKa = 11.84 VAQGQAVEE707 pKa = 4.08 ATMVHH712 pKa = 6.63 ASAPAPQPHH721 pKa = 7.31 RR722 pKa = 11.84 GPGPSDD728 pKa = 3.08 GGGGLGGHH736 pKa = 7.08 RR737 pKa = 11.84 DD738 pKa = 3.54 LVAGVGDD745 pKa = 4.13 ATAVAAGAVTVPEE758 pKa = 4.32 SGSRR762 pKa = 11.84 QTASATQQQ770 pKa = 2.89
Molecular weight: 82.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.709
IPC2_protein 6.81
IPC_protein 6.781
Toseland 6.737
ProMoST 7.263
Dawson 7.351
Bjellqvist 7.571
Wikipedia 7.293
Rodwell 7.366
Grimsley 6.839
Solomon 7.38
Lehninger 7.395
Nozaki 7.878
DTASelect 7.629
Thurlkill 7.673
EMBOSS 7.658
Sillero 7.863
Patrickios 4.113
IPC_peptide 7.38
IPC2_peptide 7.366
IPC2.peptide.svr19 7.022
Protein with the highest isoelectric point:
>tr|Q98XW9|Q98XW9_9VIRU Coat protein OS=Eimeria brunetti RNA virus 1 OX=155414 PE=4 SV=1
MM1 pKa = 6.49 MTEE4 pKa = 4.45 LLPRR8 pKa = 11.84 LTPVVAADD16 pKa = 3.81 EE17 pKa = 4.16 MGEE20 pKa = 4.18 LYY22 pKa = 10.85 LRR24 pKa = 11.84 TTFPRR29 pKa = 11.84 PPLAGTFNDD38 pKa = 3.47 TGLFPVMYY46 pKa = 10.12 AEE48 pKa = 4.35 TASLQHH54 pKa = 5.74 AVRR57 pKa = 11.84 RR58 pKa = 11.84 ILRR61 pKa = 11.84 HH62 pKa = 5.72 GPLSLTQSEE71 pKa = 4.45 CLARR75 pKa = 11.84 STFTAVGDD83 pKa = 3.71 EE84 pKa = 4.17 AVLCRR89 pKa = 11.84 RR90 pKa = 11.84 AEE92 pKa = 3.88 EE93 pKa = 4.33 AFWDD97 pKa = 3.99 LLLASEE103 pKa = 4.67 WPMHH107 pKa = 5.52 VAPVPWLLKK116 pKa = 10.6 HH117 pKa = 5.55 VVTLIARR124 pKa = 11.84 GLKK127 pKa = 9.93 QSLLEE132 pKa = 3.93 HH133 pKa = 7.44 RR134 pKa = 11.84 IPGGWIKK141 pKa = 10.52 HH142 pKa = 4.62 IRR144 pKa = 11.84 NGRR147 pKa = 11.84 SPSKK151 pKa = 10.34 EE152 pKa = 3.48 WLLGYY157 pKa = 9.12 WPRR160 pKa = 11.84 KK161 pKa = 7.34 SHH163 pKa = 6.66 PLAGKK168 pKa = 9.45 KK169 pKa = 10.37 ANLFLDD175 pKa = 4.11 CVIADD180 pKa = 3.85 FLQNYY185 pKa = 8.3 KK186 pKa = 10.75 CLGPVLEE193 pKa = 4.65 GWMKK197 pKa = 9.76 RR198 pKa = 11.84 THH200 pKa = 6.54 LLGIYY205 pKa = 9.61 EE206 pKa = 4.45 DD207 pKa = 3.79 QACNIIVYY215 pKa = 9.09 AQAVMLEE222 pKa = 4.2 FGFEE226 pKa = 3.83 EE227 pKa = 4.37 SLYY230 pKa = 9.77 LATWAVLRR238 pKa = 11.84 AKK240 pKa = 9.1 QAKK243 pKa = 9.6 ALSDD247 pKa = 3.42 VLKK250 pKa = 11.3 AMGQNMCAVGRR261 pKa = 11.84 HH262 pKa = 4.61 LCEE265 pKa = 3.84 LAVLQGRR272 pKa = 11.84 GVNRR276 pKa = 11.84 LDD278 pKa = 3.85 LLEE281 pKa = 4.44 DD282 pKa = 3.32 AEE284 pKa = 4.47 RR285 pKa = 11.84 RR286 pKa = 11.84 VSATFNCPIVHH297 pKa = 6.02 VPADD301 pKa = 3.6 ALRR304 pKa = 11.84 QCVRR308 pKa = 11.84 DD309 pKa = 3.51 VLRR312 pKa = 11.84 RR313 pKa = 11.84 EE314 pKa = 3.58 LRR316 pKa = 11.84 TRR318 pKa = 11.84 VVLPPSDD325 pKa = 3.59 DD326 pKa = 3.41 FFRR329 pKa = 11.84 RR330 pKa = 11.84 RR331 pKa = 11.84 YY332 pKa = 7.76 AWCVGGAHH340 pKa = 6.49 NLNGNDD346 pKa = 2.75 HH347 pKa = 6.85 WLPRR351 pKa = 11.84 SLLPQFPAALLWNRR365 pKa = 11.84 RR366 pKa = 11.84 AALNCVEE373 pKa = 4.37 RR374 pKa = 11.84 NPLKK378 pKa = 10.33 RR379 pKa = 11.84 WSGHH383 pKa = 3.75 VRR385 pKa = 11.84 VSVAEE390 pKa = 4.01 KK391 pKa = 10.31 VEE393 pKa = 4.03 QGKK396 pKa = 9.6 GRR398 pKa = 11.84 AIYY401 pKa = 10.67 SCDD404 pKa = 3.34 TLSYY408 pKa = 10.16 TAFSWLLEE416 pKa = 4.07 AVEE419 pKa = 4.86 KK420 pKa = 10.19 EE421 pKa = 3.83 WASRR425 pKa = 11.84 SVILNPGKK433 pKa = 10.72 GGTLGMMNRR442 pKa = 11.84 IRR444 pKa = 11.84 GAATGGRR451 pKa = 11.84 RR452 pKa = 11.84 QCYY455 pKa = 10.23 LMLDD459 pKa = 3.66 YY460 pKa = 11.49 SDD462 pKa = 5.94 FNSQHH467 pKa = 6.34 SNEE470 pKa = 4.04 AMRR473 pKa = 11.84 IVIEE477 pKa = 4.18 EE478 pKa = 4.04 TLACCDD484 pKa = 3.54 HH485 pKa = 6.78 NEE487 pKa = 4.16 PEE489 pKa = 4.66 LAAKK493 pKa = 9.74 LLKK496 pKa = 10.83 SIDD499 pKa = 3.72 DD500 pKa = 3.27 MHH502 pKa = 7.49 IYY504 pKa = 10.47 LRR506 pKa = 11.84 GVHH509 pKa = 6.42 IGRR512 pKa = 11.84 VAGSLMSGHH521 pKa = 7.32 RR522 pKa = 11.84 ATTYY526 pKa = 9.54 WNSVLNAAYY535 pKa = 9.92 VRR537 pKa = 11.84 YY538 pKa = 9.98 AIGRR542 pKa = 11.84 VAYY545 pKa = 7.72 DD546 pKa = 3.22 TYY548 pKa = 11.64 KK549 pKa = 10.35 PFHH552 pKa = 7.33 VGDD555 pKa = 4.3 DD556 pKa = 3.74 VLILLDD562 pKa = 4.27 SPADD566 pKa = 3.1 AWNVVKK572 pKa = 10.62 KK573 pKa = 10.56 LEE575 pKa = 5.02 HH576 pKa = 6.54 IGCTLQRR583 pKa = 11.84 SKK585 pKa = 11.21 QSVGIGGYY593 pKa = 9.95 EE594 pKa = 4.13 FLRR597 pKa = 11.84 VAGSPFTGVGGYY609 pKa = 7.1 VARR612 pKa = 11.84 SVAGLVSGSWVSSVKK627 pKa = 9.41 MAPRR631 pKa = 11.84 EE632 pKa = 4.0 ALQSLIQQARR642 pKa = 11.84 SIMNRR647 pKa = 11.84 SGNPAAYY654 pKa = 9.35 KK655 pKa = 10.57 LLISSARR662 pKa = 11.84 RR663 pKa = 11.84 TIGIDD668 pKa = 3.16 QQYY671 pKa = 10.86 LEE673 pKa = 4.98 EE674 pKa = 4.26 FLSGNVALAPGPCYY688 pKa = 10.26 RR689 pKa = 11.84 SDD691 pKa = 3.04 RR692 pKa = 11.84 RR693 pKa = 11.84 YY694 pKa = 9.48 VARR697 pKa = 11.84 HH698 pKa = 5.05 VEE700 pKa = 4.2 WEE702 pKa = 4.2 SSADD706 pKa = 3.54 DD707 pKa = 4.16 PYY709 pKa = 11.77 GGGRR713 pKa = 11.84 LLIGGIEE720 pKa = 4.05 IEE722 pKa = 4.44 KK723 pKa = 10.33 LPNHH727 pKa = 7.16 AARR730 pKa = 11.84 DD731 pKa = 3.97 YY732 pKa = 10.79 VNWAASEE739 pKa = 4.24 VEE741 pKa = 4.3 RR742 pKa = 11.84 VGLAMVGRR750 pKa = 11.84 VPWRR754 pKa = 11.84 AMALTAYY761 pKa = 10.51 AAMEE765 pKa = 4.1 PSLYY769 pKa = 10.37 GNPSGGVRR777 pKa = 11.84 AAYY780 pKa = 9.48 ISPRR784 pKa = 11.84 TVRR787 pKa = 11.84 LIDD790 pKa = 3.86 HH791 pKa = 6.97 YY792 pKa = 11.26 IDD794 pKa = 3.96 AADD797 pKa = 3.38 ITTTDD802 pKa = 3.39 SQHH805 pKa = 6.92 GILAQYY811 pKa = 9.71 PLLSLLRR818 pKa = 11.84 NYY820 pKa = 10.43 FSMEE824 pKa = 3.98 DD825 pKa = 3.33 LTYY828 pKa = 11.28 LLNFVGRR835 pKa = 11.84 DD836 pKa = 3.13 AGIDD840 pKa = 3.48 ARR842 pKa = 11.84 VTAWGGTNEE851 pKa = 4.68 GICVCGLLPYY861 pKa = 10.72 ADD863 pKa = 3.85 AAGAASRR870 pKa = 11.84 VLGNAIHH877 pKa = 6.22 VSVPVAVV884 pKa = 3.66
Molecular weight: 97.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.856
IPC2_protein 8.039
IPC_protein 7.849
Toseland 7.483
ProMoST 8.419
Dawson 8.551
Bjellqvist 8.902
Wikipedia 8.492
Rodwell 8.58
Grimsley 7.541
Solomon 8.595
Lehninger 8.595
Nozaki 9.033
DTASelect 8.624
Thurlkill 8.668
EMBOSS 8.726
Sillero 8.975
Patrickios 4.266
IPC_peptide 8.595
IPC2_peptide 8.024
IPC2.peptide.svr19 8.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1654
770
884
827.0
90.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.883 ± 0.097
2.237 ± 0.063
4.232 ± 0.129
5.079 ± 0.009
2.842 ± 0.334
8.706 ± 0.483
2.902 ± 0.053
3.567 ± 0.524
2.842 ± 0.071
10.218 ± 0.946
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.6 ± 0.161
3.567 ± 0.038
5.381 ± 0.532
2.66 ± 0.042
7.376 ± 0.551
5.925 ± 0.213
5.139 ± 0.926
8.585 ± 0.478
1.874 ± 0.359
3.386 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here