spirochete symbiont of Stewartia floridana
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 817 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A421K8W2|A0A421K8W2_9SPIR Uncharacterized protein OS=spirochete symbiont of Stewartia floridana OX=1968597 GN=B6D68_03135 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.15 NYY4 pKa = 10.45 QNITLSLLVFTLFWGCQASLPDD26 pKa = 3.6 NLEE29 pKa = 4.01 TLGEE33 pKa = 4.35 SGLAWEE39 pKa = 4.78 MSTLTSEE46 pKa = 5.38 DD47 pKa = 3.81 DD48 pKa = 4.39 LNVLLARR55 pKa = 11.84 SGEE58 pKa = 4.41 GIVDD62 pKa = 3.45 WQLSRR67 pKa = 11.84 EE68 pKa = 4.14 FAAMALEE75 pKa = 4.03 EE76 pKa = 5.25 FIEE79 pKa = 4.3 EE80 pKa = 4.05 DD81 pKa = 3.38 NYY83 pKa = 10.98 PPEE86 pKa = 5.16 SEE88 pKa = 4.77 LWDD91 pKa = 3.61 IPIAIYY97 pKa = 10.56 DD98 pKa = 3.77 DD99 pKa = 3.91 EE100 pKa = 6.46 GEE102 pKa = 3.95 VRR104 pKa = 11.84 YY105 pKa = 10.27 YY106 pKa = 10.42 EE107 pKa = 3.95 FRR109 pKa = 11.84 IVDD112 pKa = 3.92 GEE114 pKa = 4.24 QTLGAIAGNARR125 pKa = 11.84 EE126 pKa = 4.33 DD127 pKa = 3.13 RR128 pKa = 11.84 GAPVSHH134 pKa = 6.2 VFEE137 pKa = 4.18 MDD139 pKa = 3.57 GYY141 pKa = 11.19 ADD143 pKa = 4.12 EE144 pKa = 5.72 LSALYY149 pKa = 10.58 ASGAMSADD157 pKa = 4.43 DD158 pKa = 3.72 IPRR161 pKa = 11.84 IVDD164 pKa = 3.24 NDD166 pKa = 3.71 YY167 pKa = 10.79 PSYY170 pKa = 11.26 AVGSVTLTRR179 pKa = 11.84 SGNVDD184 pKa = 3.3 LKK186 pKa = 11.11 EE187 pKa = 4.06 IVDD190 pKa = 4.0 PSTGEE195 pKa = 3.65 QLEE198 pKa = 4.29 NLVVLLDD205 pKa = 3.83 MEE207 pKa = 4.49 EE208 pKa = 4.66 SIAAYY213 pKa = 9.57 PDD215 pKa = 3.41 TT216 pKa = 4.74
Molecular weight: 23.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.363
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.935
Thurlkill 3.49
EMBOSS 3.554
Sillero 3.77
Patrickios 1.812
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A421K9E7|A0A421K9E7_9SPIR Uncharacterized protein OS=spirochete symbiont of Stewartia floridana OX=1968597 GN=B6D68_02115 PE=4 SV=1
MM1 pKa = 7.08 SQTRR5 pKa = 11.84 SLAGLLISIVFMAILCSPQALHH27 pKa = 7.01 PVLQTLFPQAGEE39 pKa = 4.13 WVFPRR44 pKa = 11.84 ATLIRR49 pKa = 11.84 LTIQHH54 pKa = 6.48 LTLSLTAAGIAALIGILLAITATRR78 pKa = 11.84 RR79 pKa = 11.84 HH80 pKa = 5.12 GRR82 pKa = 11.84 AFLRR86 pKa = 11.84 MIRR89 pKa = 11.84 DD90 pKa = 3.69 VSSLAQTIPPTAVLALAIPFLGFGYY115 pKa = 10.4 KK116 pKa = 9.64 PVLLALVLYY125 pKa = 10.67 SILPVLNNTLTALEE139 pKa = 4.81 CLPKK143 pKa = 10.31 PILEE147 pKa = 4.56 AAQAMGMKK155 pKa = 9.88 SLQVLFLIEE164 pKa = 4.8 LPLALPAIGTGIRR177 pKa = 11.84 ISAVINIGTATIAALAGAGGLGSPIVAGLVRR208 pKa = 11.84 DD209 pKa = 3.76 NPAWVFQGAASSALLALILNAILLRR234 pKa = 11.84 VEE236 pKa = 4.78 VKK238 pKa = 10.79 LNPAKK243 pKa = 10.71
Molecular weight: 25.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.663
IPC_protein 10.54
Toseland 10.804
ProMoST 10.613
Dawson 10.877
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 11.052
Grimsley 10.921
Solomon 11.038
Lehninger 11.008
Nozaki 10.804
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.818
IPC_peptide 11.052
IPC2_peptide 9.765
IPC2.peptide.svr19 8.653
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
817
0
817
230933
27
1508
282.7
31.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.175 ± 0.093
1.121 ± 0.037
5.492 ± 0.065
6.437 ± 0.068
3.978 ± 0.06
8.227 ± 0.068
2.131 ± 0.039
6.909 ± 0.067
4.527 ± 0.067
10.049 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.724 ± 0.041
3.432 ± 0.046
4.64 ± 0.058
2.901 ± 0.046
6.537 ± 0.075
6.479 ± 0.055
4.694 ± 0.054
6.462 ± 0.071
1.287 ± 0.034
2.796 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here