Idiomarina tyrosinivorans
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2274 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432ZQU4|A0A432ZQU4_9GAMM GTPase Der OS=Idiomarina tyrosinivorans OX=1445662 GN=der PE=3 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.1 FLHH5 pKa = 6.69 SLTAIAVAAALSACGGDD22 pKa = 3.73 DD23 pKa = 3.95 EE24 pKa = 6.54 KK25 pKa = 11.62 NVAPIATDD33 pKa = 3.49 DD34 pKa = 4.18 SYY36 pKa = 11.52 TATVGATNTIDD47 pKa = 3.87 VLANDD52 pKa = 3.93 SDD54 pKa = 4.47 EE55 pKa = 4.27 NTKK58 pKa = 9.44 DD59 pKa = 3.55 TLSVTAVTQPDD70 pKa = 3.83 SGSVTINSDD79 pKa = 3.03 GTLSFEE85 pKa = 4.7 PSGDD89 pKa = 3.52 TLGDD93 pKa = 3.38 DD94 pKa = 3.44 SFTYY98 pKa = 9.6 TVSDD102 pKa = 4.35 GEE104 pKa = 4.45 LDD106 pKa = 3.64 DD107 pKa = 4.14 TATVSLTVQQALAFSGKK124 pKa = 9.89 VVDD127 pKa = 4.39 AAIPNATVTITIGEE141 pKa = 4.31 DD142 pKa = 3.35 TFTTTADD149 pKa = 3.28 EE150 pKa = 4.78 DD151 pKa = 4.06 GNYY154 pKa = 8.84 TLEE157 pKa = 3.94 VFYY160 pKa = 10.86 TDD162 pKa = 2.97 GTKK165 pKa = 10.11 LVKK168 pKa = 10.19 IEE170 pKa = 4.25 AVGSADD176 pKa = 3.78 NEE178 pKa = 4.0 QDD180 pKa = 4.08 HH181 pKa = 6.92 VDD183 pKa = 4.03 LTSLMPSLATLQGLAGDD200 pKa = 4.07 DD201 pKa = 4.43 AIVQRR206 pKa = 11.84 SEE208 pKa = 4.18 AGGTNVTNVTTASYY222 pKa = 11.52 VLTKK226 pKa = 9.87 EE227 pKa = 4.33 ANGGEE232 pKa = 4.19 DD233 pKa = 3.42 PADD236 pKa = 3.89 EE237 pKa = 4.41 NTLSAAEE244 pKa = 4.15 SNIDD248 pKa = 3.27 GTKK251 pKa = 9.75 VLEE254 pKa = 3.88 IAAVIKK260 pKa = 10.34 VIVDD264 pKa = 3.44 NPDD267 pKa = 2.76 FDD269 pKa = 5.81 LPDD272 pKa = 3.77 GVSTIGEE279 pKa = 4.69 LINDD283 pKa = 3.32 EE284 pKa = 4.27 TAYY287 pKa = 10.79 NDD289 pKa = 3.14 YY290 pKa = 10.73 VAYY293 pKa = 10.69 VEE295 pKa = 4.43 NTDD298 pKa = 3.52 PTALQAAADD307 pKa = 4.52 AIVEE311 pKa = 4.18 DD312 pKa = 4.56 EE313 pKa = 4.4 EE314 pKa = 4.5 LTVASTQFKK323 pKa = 10.81 LPAFYY328 pKa = 10.23 IQLPTTQPGFIARR341 pKa = 11.84 GDD343 pKa = 3.53 STLYY347 pKa = 10.98 FNDD350 pKa = 4.83 DD351 pKa = 3.24 GTGVYY356 pKa = 10.3 INGFFGLEE364 pKa = 3.97 NEE366 pKa = 4.79 TDD368 pKa = 4.35 FTWSRR373 pKa = 11.84 DD374 pKa = 3.61 DD375 pKa = 3.53 NGYY378 pKa = 9.89 YY379 pKa = 10.81 ALDD382 pKa = 3.74 FATPAVYY389 pKa = 9.45 IDD391 pKa = 4.0 YY392 pKa = 8.91 PAVTDD397 pKa = 3.69 EE398 pKa = 4.67 TDD400 pKa = 3.29 DD401 pKa = 3.74 ATILAAYY408 pKa = 9.6 DD409 pKa = 3.68 DD410 pKa = 4.13 ASVYY414 pKa = 9.51 QVEE417 pKa = 4.74 TEE419 pKa = 4.22 VSVSALAFKK428 pKa = 10.94 LLVDD432 pKa = 4.77 GKK434 pKa = 9.08 TVDD437 pKa = 3.62 LVQAKK442 pKa = 7.42 EE443 pKa = 4.44 TYY445 pKa = 8.73 TKK447 pKa = 10.52 VYY449 pKa = 10.42 QPLNGVDD456 pKa = 3.11 GKK458 pKa = 9.41 TYY460 pKa = 10.1 QIPTAQGVRR469 pKa = 11.84 KK470 pKa = 8.97 FQYY473 pKa = 10.5 SYY475 pKa = 11.71 LNGDD479 pKa = 4.64 ALPKK483 pKa = 10.2 IAGTEE488 pKa = 3.84 ADD490 pKa = 4.09 LTTQQWVLPIVGLAAPGDD508 pKa = 3.76 MHH510 pKa = 7.24 EE511 pKa = 4.9 ALQPDD516 pKa = 5.52 LITFNSDD523 pKa = 2.38 GSFGSDD529 pKa = 3.31 YY530 pKa = 11.4 AGVDD534 pKa = 4.23 GEE536 pKa = 4.38 WSLTDD541 pKa = 4.06 SIISMSYY548 pKa = 8.82 TSEE551 pKa = 3.79 EE552 pKa = 4.1 FGDD555 pKa = 3.98 VEE557 pKa = 4.29 QKK559 pKa = 10.45 VQIVSTKK566 pKa = 9.99 NGVYY570 pKa = 8.8 TAFVEE575 pKa = 4.92 VSSEE579 pKa = 3.75 AANATWYY586 pKa = 10.45 DD587 pKa = 3.28 WVAPRR592 pKa = 11.84 DD593 pKa = 3.74 PEE595 pKa = 4.26 YY596 pKa = 10.79 TIDD599 pKa = 4.47 PADD602 pKa = 4.11 LVNTAATMWLTNPNIWYY619 pKa = 10.06 ASQWDD624 pKa = 3.72 QAEE627 pKa = 4.17 NLPSVFTILGWQFDD641 pKa = 4.5 EE642 pKa = 4.62 AGKK645 pKa = 10.49 AYY647 pKa = 7.59 RR648 pKa = 11.84 TSVGCQEE655 pKa = 4.24 YY656 pKa = 10.82 YY657 pKa = 10.07 IDD659 pKa = 4.15 GTCTYY664 pKa = 11.03 DD665 pKa = 3.12 STLVVLNNSTRR676 pKa = 11.84 NYY678 pKa = 10.44 RR679 pKa = 11.84 VIEE682 pKa = 4.48 DD683 pKa = 3.21 SSGQQRR689 pKa = 11.84 IQMAYY694 pKa = 9.97 NFEE697 pKa = 4.34 CFGAGNQVDD706 pKa = 4.49 ANCAAYY712 pKa = 9.68 EE713 pKa = 3.95 YY714 pKa = 10.53 IPLQEE719 pKa = 4.71 AEE721 pKa = 4.36 NGQITLLEE729 pKa = 4.32 SVFVDD734 pKa = 4.2 RR735 pKa = 11.84 DD736 pKa = 3.01 PSTPVNLVSGFTPRR750 pKa = 11.84 VTVSIPFEE758 pKa = 4.03 FPQGIEE764 pKa = 3.62 NSAFPGATASSGQAMPAGKK783 pKa = 9.77 VDD785 pKa = 3.35 RR786 pKa = 11.84 VSFLGKK792 pKa = 8.62 TKK794 pKa = 10.47 SAPLVINHH802 pKa = 7.01
Molecular weight: 85.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.389
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.834
Patrickios 0.896
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A432ZTA2|A0A432ZTA2_9GAMM Peptidase M23 OS=Idiomarina tyrosinivorans OX=1445662 GN=CWI84_03185 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.96 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.91 VLSAA44 pKa = 4.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2274
0
2274
746298
35
2761
328.2
36.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.859 ± 0.054
0.882 ± 0.017
5.667 ± 0.048
6.125 ± 0.052
3.757 ± 0.034
6.863 ± 0.054
2.258 ± 0.029
5.578 ± 0.036
4.367 ± 0.045
10.556 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.027
3.645 ± 0.041
4.134 ± 0.035
5.854 ± 0.059
5.597 ± 0.04
5.987 ± 0.046
5.046 ± 0.038
7.11 ± 0.045
1.42 ± 0.025
2.897 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here