Lactiplantibacillus mudanjiangensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactiplantibacillus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660E4B9|A0A660E4B9_9LACO DNA-binding protein XRE family [Lactobacillus sakei subsp. sakei 23K] OS=Lactiplantibacillus mudanjiangensis OX=1296538 GN=MUDAN_MDHGFNIF_01789 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 3.39QLVLEE8 pKa = 4.39TQKK11 pKa = 10.47WLNATYY17 pKa = 11.07GDD19 pKa = 3.61VSGFGSVPEE28 pKa = 4.83DD29 pKa = 4.47GFTGWDD35 pKa = 3.12TVYY38 pKa = 11.3GLTRR42 pKa = 11.84ALQHH46 pKa = 6.13EE47 pKa = 4.73LGITDD52 pKa = 4.26LVDD55 pKa = 3.26NFGPTTASLFDD66 pKa = 4.77EE67 pKa = 4.75IADD70 pKa = 4.29EE71 pKa = 4.2IVPGYY76 pKa = 9.57SGNIAYY82 pKa = 9.53IIQGGFWCKK91 pKa = 10.65GIDD94 pKa = 3.4PVAFDD99 pKa = 5.08GEE101 pKa = 4.5FSSDD105 pKa = 3.25TEE107 pKa = 4.56SAVSTMKK114 pKa = 10.47EE115 pKa = 3.82YY116 pKa = 10.99AGLSDD121 pKa = 3.77TSGTLNSQFMAALLNMSAFTLVADD145 pKa = 5.1GYY147 pKa = 10.3EE148 pKa = 4.68KK149 pKa = 10.39IRR151 pKa = 11.84TMQQQLNHH159 pKa = 7.5DD160 pKa = 4.0YY161 pKa = 10.93LAYY164 pKa = 9.73TGILPCDD171 pKa = 4.69GIYY174 pKa = 10.55QRR176 pKa = 11.84DD177 pKa = 3.81TNTALLYY184 pKa = 10.65ALQAEE189 pKa = 4.68EE190 pKa = 5.61GLDD193 pKa = 3.43TDD195 pKa = 4.49TASGTYY201 pKa = 10.85GPMTQEE207 pKa = 3.89LTPTVYY213 pKa = 10.71EE214 pKa = 4.19GDD216 pKa = 3.56TNNFVRR222 pKa = 11.84ILQWGLYY229 pKa = 9.56VNNQTYY235 pKa = 9.41TGDD238 pKa = 3.41FDD240 pKa = 3.77GTYY243 pKa = 10.86SLDD246 pKa = 3.32VSSAVSTFEE255 pKa = 3.84TDD257 pKa = 2.81MALPSTSGASAGLDD271 pKa = 3.05VFMSLLTSAGNPNRR285 pKa = 11.84AAVACDD291 pKa = 3.21TSHH294 pKa = 7.23QINASEE300 pKa = 3.94AAALNTAGYY309 pKa = 9.23EE310 pKa = 4.01YY311 pKa = 10.8VGRR314 pKa = 11.84YY315 pKa = 7.02LTGTAGTGDD324 pKa = 3.56NEE326 pKa = 4.24VAKK329 pKa = 10.77NLTTTEE335 pKa = 3.77ISTLTDD341 pKa = 3.06AGLKK345 pKa = 9.86IFPIYY350 pKa = 9.99QDD352 pKa = 3.47GASDD356 pKa = 3.79SEE358 pKa = 5.21DD359 pKa = 3.67YY360 pKa = 9.54FTEE363 pKa = 4.38AKK365 pKa = 10.3GKK367 pKa = 10.27SDD369 pKa = 3.17GRR371 pKa = 11.84KK372 pKa = 9.43AGLAAMEE379 pKa = 5.15LGFPDD384 pKa = 4.31DD385 pKa = 4.63AIIYY389 pKa = 7.6FAVDD393 pKa = 2.75VDD395 pKa = 3.88IQDD398 pKa = 3.32GDD400 pKa = 3.54IAGTIAPYY408 pKa = 8.63FTGVASGIAGFDD420 pKa = 3.81FNVGIYY426 pKa = 8.04ATRR429 pKa = 11.84NVVNTIITDD438 pKa = 4.14GLAEE442 pKa = 4.26KK443 pKa = 10.83AFVSDD448 pKa = 3.63MSTGFSGNLGFAMPTEE464 pKa = 3.94WAFDD468 pKa = 3.57QFSEE472 pKa = 4.72VAFDD476 pKa = 4.93DD477 pKa = 4.82FYY479 pKa = 11.11IDD481 pKa = 3.9KK482 pKa = 10.61VASNSSRR489 pKa = 11.84STAVSDD495 pKa = 4.08FTAGGAVGGADD506 pKa = 4.2DD507 pKa = 4.12LAKK510 pKa = 10.41INEE513 pKa = 4.57IISDD517 pKa = 4.05LGDD520 pKa = 3.32TDD522 pKa = 5.66SIFNFVKK529 pKa = 10.35DD530 pKa = 3.78VKK532 pKa = 10.7IEE534 pKa = 4.06ATSEE538 pKa = 4.26EE539 pKa = 4.77YY540 pKa = 10.64KK541 pKa = 9.79VTTPAMDD548 pKa = 4.53LSLKK552 pKa = 10.32VDD554 pKa = 4.13LEE556 pKa = 4.38GSISDD561 pKa = 4.42DD562 pKa = 4.74DD563 pKa = 5.25GDD565 pKa = 3.78TSVTYY570 pKa = 10.42NVNDD574 pKa = 4.08GSVHH578 pKa = 6.48VDD580 pKa = 3.16LGDD583 pKa = 5.78DD584 pKa = 3.55ISQLLAEE591 pKa = 4.41NTAGFTDD598 pKa = 3.85TAVTASLDD606 pKa = 3.81SVGSVIKK613 pKa = 10.86NGEE616 pKa = 4.07IKK618 pKa = 10.98NKK620 pKa = 9.73IDD622 pKa = 3.36VSATATTITFKK633 pKa = 10.91LDD635 pKa = 3.1STFEE639 pKa = 4.15HH640 pKa = 7.05EE641 pKa = 4.74SNNGKK646 pKa = 8.07TYY648 pKa = 9.92EE649 pKa = 3.94IEE651 pKa = 4.11YY652 pKa = 10.32EE653 pKa = 3.82ITLVVSFHH661 pKa = 7.11SIITPLPDD669 pKa = 3.86WITATEE675 pKa = 3.86ADD677 pKa = 3.66AHH679 pKa = 6.1NSALTEE685 pKa = 3.96IQGDD689 pKa = 3.64AVRR692 pKa = 11.84IMEE695 pKa = 4.5GAAVVVGLAIGAIAIAGLIASGTFVVGYY723 pKa = 10.15LVDD726 pKa = 4.11AVALAVGAII735 pKa = 3.8

Molecular weight: 77.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660DY40|A0A660DY40_9LACO Uncharacterized protein OS=Lactiplantibacillus mudanjiangensis OX=1296538 GN=MUDAN_MDHGFNIF_00232 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.69KK9 pKa = 7.77RR10 pKa = 11.84HH11 pKa = 4.99RR12 pKa = 11.84QRR14 pKa = 11.84VHH16 pKa = 6.07GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.8VLSAA44 pKa = 4.11

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3503

0

3503

1003855

29

5304

286.6

31.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.108 ± 0.052

0.466 ± 0.01

5.684 ± 0.05

4.423 ± 0.047

3.972 ± 0.029

6.643 ± 0.04

2.254 ± 0.022

6.362 ± 0.038

5.569 ± 0.05

10.159 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.024

4.383 ± 0.033

3.729 ± 0.023

5.324 ± 0.045

3.99 ± 0.037

5.767 ± 0.048

7.438 ± 0.074

7.27 ± 0.033

1.239 ± 0.02

3.565 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski