Nocardioides anomalus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6WKQ3|A0A6G6WKQ3_9ACTN DNA internalization-related competence protein ComEC/Rec2 OS=Nocardioides anomalus OX=2712223 GN=G5V58_07830 PE=4 SV=1
MM1 pKa = 7.16GAALGFSLVACGDD14 pKa = 4.02DD15 pKa = 4.23SDD17 pKa = 5.88SGDD20 pKa = 3.69DD21 pKa = 3.54TSGSDD26 pKa = 3.51EE27 pKa = 4.38SSSASADD34 pKa = 3.46TSEE37 pKa = 4.59SASEE41 pKa = 4.2SASADD46 pKa = 3.13AGGSFTNDD54 pKa = 2.31KK55 pKa = 10.72CAGSDD60 pKa = 3.08TSANKK65 pKa = 9.76FAVGGILPLTGNLAFLGPPAVAGVGLAVSDD95 pKa = 3.7INAAGGVNGAQACSNILDD113 pKa = 4.54SGDD116 pKa = 3.73STDD119 pKa = 3.8MSISTASAGTMVAAKK134 pKa = 9.2PSVVIGAEE142 pKa = 4.09SSSVSLNFVDD152 pKa = 4.02TLTDD156 pKa = 3.42AKK158 pKa = 9.61ITQISPANTAIDD170 pKa = 3.83LSGYY174 pKa = 9.48SPFYY178 pKa = 10.71FRR180 pKa = 11.84TAPPDD185 pKa = 3.84TVQGNALGTLISQDD199 pKa = 3.15GYY201 pKa = 9.09TNIGFLVFNDD211 pKa = 3.95TYY213 pKa = 11.22GTGLRR218 pKa = 11.84NAVQQTVEE226 pKa = 3.91AAGGKK231 pKa = 9.05CVYY234 pKa = 9.91GCKK237 pKa = 10.13GDD239 pKa = 4.08GDD241 pKa = 4.34EE242 pKa = 5.39FPAGQTTFSAEE253 pKa = 4.16VQAVTAAKK261 pKa = 9.51PDD263 pKa = 4.43AIVIIAFDD271 pKa = 3.8EE272 pKa = 4.54TKK274 pKa = 10.81SIVPEE279 pKa = 4.08LASSGFDD286 pKa = 3.01MSKK289 pKa = 9.61TYY291 pKa = 10.4FVDD294 pKa = 4.82GNLSDD299 pKa = 3.94YY300 pKa = 11.2GQDD303 pKa = 3.73FEE305 pKa = 5.7PGTLEE310 pKa = 4.3GAQGTLPGQDD320 pKa = 3.56PDD322 pKa = 4.72QGFKK326 pKa = 11.28DD327 pKa = 4.64NLTGWYY333 pKa = 9.79KK334 pKa = 10.48SANGEE339 pKa = 4.1DD340 pKa = 3.81LKK342 pKa = 11.15DD343 pKa = 3.54YY344 pKa = 11.1SYY346 pKa = 11.63GAEE349 pKa = 4.32SYY351 pKa = 10.87DD352 pKa = 3.59ATILAALAAAKK363 pKa = 10.41GGANDD368 pKa = 3.56SQTIQKK374 pKa = 9.87NLAAVSGATDD384 pKa = 3.78GEE386 pKa = 4.49EE387 pKa = 3.89CSTYY391 pKa = 11.22ADD393 pKa = 3.8CLKK396 pKa = 10.63LIEE399 pKa = 4.98GGSDD403 pKa = 2.54IHH405 pKa = 5.63YY406 pKa = 7.36TGPSGIGPLDD416 pKa = 4.33DD417 pKa = 5.31EE418 pKa = 5.29NDD420 pKa = 3.62PSSAFIGIYY429 pKa = 10.17QYY431 pKa = 11.36DD432 pKa = 3.93ANNKK436 pKa = 9.17NILSGTVEE444 pKa = 4.1GSKK447 pKa = 10.86PEE449 pKa = 3.83

Molecular weight: 45.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6W969|A0A6G6W969_9ACTN ROK family protein OS=Nocardioides anomalus OX=2712223 GN=G5V58_03015 PE=3 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84SHH5 pKa = 6.66LLKK8 pKa = 10.38ALQPARR14 pKa = 11.84VTGRR18 pKa = 11.84RR19 pKa = 11.84GRR21 pKa = 11.84GRR23 pKa = 11.84GRR25 pKa = 11.84GRR27 pKa = 11.84GHH29 pKa = 7.07TSTGTHH35 pKa = 6.43TGTGTRR41 pKa = 11.84TRR43 pKa = 11.84TRR45 pKa = 11.84THH47 pKa = 5.77GG48 pKa = 3.3

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4914

0

4914

1544052

33

2111

314.2

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.478 ± 0.063

0.711 ± 0.009

6.56 ± 0.033

5.695 ± 0.037

2.729 ± 0.019

9.375 ± 0.037

2.218 ± 0.016

2.816 ± 0.025

1.756 ± 0.025

10.564 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.637 ± 0.014

1.57 ± 0.021

5.74 ± 0.03

2.856 ± 0.017

7.695 ± 0.037

5.119 ± 0.024

6.144 ± 0.036

9.789 ± 0.037

1.562 ± 0.015

1.989 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski