Endozoicomonas elysicola
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A081K8W0|A0A081K8W0_9GAMM Uncharacterized protein OS=Endozoicomonas elysicola OX=305900 GN=GV64_07395 PE=4 SV=1
MM1 pKa = 7.26 NRR3 pKa = 11.84 NDD5 pKa = 4.29 LFRR8 pKa = 11.84 KK9 pKa = 9.7 SLLSTAVVAVLHH21 pKa = 6.14 TYY23 pKa = 11.49 SMGAAAACIPEE34 pKa = 4.35 NVDD37 pKa = 3.26 GVNTIIVDD45 pKa = 3.84 DD46 pKa = 4.45 GTDD49 pKa = 3.63 CGDD52 pKa = 3.6 ISITTPDD59 pKa = 3.71 NVNHH63 pKa = 6.61 IEE65 pKa = 4.01 VEE67 pKa = 4.61 GIVTGDD73 pKa = 3.03 ITNATDD79 pKa = 4.25 LSFLKK84 pKa = 10.38 IEE86 pKa = 4.66 AGDD89 pKa = 3.72 VGEE92 pKa = 4.46 GWIQGSVINQGDD104 pKa = 3.68 ISHH107 pKa = 7.12 HH108 pKa = 6.44 DD109 pKa = 3.26 VHH111 pKa = 6.59 KK112 pKa = 11.04 ASFSLSGPKK121 pKa = 9.87 EE122 pKa = 4.07 LLTVDD127 pKa = 4.44 GDD129 pKa = 4.02 VLNAGSATYY138 pKa = 8.84 MQVEE142 pKa = 4.32 HH143 pKa = 7.53 ADD145 pKa = 3.16 IGGDD149 pKa = 3.67 IINSGQLTDD158 pKa = 5.54 DD159 pKa = 4.08 FLLVDD164 pKa = 3.72 DD165 pKa = 5.08 TIVYY169 pKa = 10.25 GSVINTEE176 pKa = 3.97 SGTIGSAEE184 pKa = 3.91 NSVYY188 pKa = 10.81 DD189 pKa = 5.63 AILLNDD195 pKa = 3.74 AVIKK199 pKa = 10.67 EE200 pKa = 4.11 GVINNGTITGEE211 pKa = 3.78 NSGILISKK219 pKa = 9.98 SEE221 pKa = 3.76 ITGTQEE227 pKa = 3.58 IEE229 pKa = 4.25 GVLGAVVNNGDD240 pKa = 3.64 INLGTGGKK248 pKa = 8.94 EE249 pKa = 3.6 YY250 pKa = 10.6 AGRR253 pKa = 11.84 GMFGIQITNAMPLDD267 pKa = 4.07 APGGSEE273 pKa = 4.1 DD274 pKa = 4.21 QEE276 pKa = 4.16 IATSIISGSVINTGNITVTDD296 pKa = 4.69 GAPEE300 pKa = 4.12 EE301 pKa = 4.56 PQLQDD306 pKa = 3.43 DD307 pKa = 4.47 RR308 pKa = 11.84 FGGIYY313 pKa = 9.66 IGSEE317 pKa = 3.45 WRR319 pKa = 11.84 GKK321 pKa = 10.59 EE322 pKa = 3.89 DD323 pKa = 3.93 DD324 pKa = 3.95 SVAAHH329 pKa = 5.88 VKK331 pKa = 10.6 GNVINTGTIDD341 pKa = 3.65 VSVTNTDD348 pKa = 2.96 RR349 pKa = 11.84 GEE351 pKa = 4.14 TFGILIEE358 pKa = 4.05 NAIVDD363 pKa = 4.09 GTVGNIGIDD372 pKa = 3.62 SLITTSRR379 pKa = 11.84 EE380 pKa = 3.81 GIIVVDD386 pKa = 4.58 GSDD389 pKa = 3.02 VGDD392 pKa = 3.67 IVNEE396 pKa = 3.91 GTITVTEE403 pKa = 4.63 FWDD406 pKa = 4.82 GIYY409 pKa = 10.46 VDD411 pKa = 4.37 SSSAASISNSGSITTTAGSGIYY433 pKa = 10.1 LDD435 pKa = 3.77 AAVVEE440 pKa = 4.55 GTIRR444 pKa = 11.84 NDD446 pKa = 3.62 GQITANGAYY455 pKa = 9.22 IEE457 pKa = 4.57 YY458 pKa = 9.62 PVGDD462 pKa = 4.1 YY463 pKa = 10.98 EE464 pKa = 4.47 PASEE468 pKa = 4.27 YY469 pKa = 11.06 LDD471 pKa = 3.61 GSGIFVSAYY480 pKa = 9.56 GEE482 pKa = 4.14 CDD484 pKa = 3.35 EE485 pKa = 5.84 EE486 pKa = 6.01 YY487 pKa = 10.42 DD488 pKa = 4.37 DD489 pKa = 6.43 CPITPSYY496 pKa = 10.0 VGSIINGEE504 pKa = 3.99 NGVINATEE512 pKa = 3.84 AGIDD516 pKa = 3.43 IDD518 pKa = 3.85 HH519 pKa = 6.46 SVIGSISNLGKK530 pKa = 10.12 IVADD534 pKa = 3.54 RR535 pKa = 11.84 VGIYY539 pKa = 10.5 AEE541 pKa = 4.16 NGTIEE546 pKa = 4.55 GNITNSGLIEE556 pKa = 4.62 ADD558 pKa = 3.06 GDD560 pKa = 4.49 GISLMGVEE568 pKa = 4.26 ATGIVNLGSITAHH581 pKa = 6.37 NGLGIYY587 pKa = 10.44 SSGSNLTDD595 pKa = 4.74 GIYY598 pKa = 10.65 NDD600 pKa = 4.3 GQITSDD606 pKa = 3.67 GPGIIAVSYY615 pKa = 10.46 SPEE618 pKa = 3.71 EE619 pKa = 3.92 TVQVGFIANDD629 pKa = 3.33 EE630 pKa = 4.43 NGVITADD637 pKa = 3.5 YY638 pKa = 10.23 GPGIGAFAVIGEE650 pKa = 4.41 SLANTGTINAEE661 pKa = 3.98 SGIVAMLSTLTDD673 pKa = 3.56 GVVNTGTINADD684 pKa = 2.8 WVGIQLDD691 pKa = 3.94 TTQAGTVANQGTINVSADD709 pKa = 3.01 YY710 pKa = 10.54 GAAIDD715 pKa = 3.85 IRR717 pKa = 11.84 GSKK720 pKa = 8.56 LTTGVYY726 pKa = 11.01 NNGTLNASEE735 pKa = 5.3 GILASGYY742 pKa = 9.82 PDD744 pKa = 4.48 FGPVRR749 pKa = 11.84 AAEE752 pKa = 4.62 DD753 pKa = 4.3 LPPPPPPPGPVPLEE767 pKa = 3.78 EE768 pKa = 4.15 TAPLSIGSVVNDD780 pKa = 3.33 EE781 pKa = 4.97 DD782 pKa = 4.39 GVINATAEE790 pKa = 3.83 DD791 pKa = 4.22 SYY793 pKa = 11.75 GIGLEE798 pKa = 3.99 FVEE801 pKa = 5.55 VVGLGNAGIINASYY815 pKa = 10.44 GLNVYY820 pKa = 10.2 EE821 pKa = 6.1 SILTDD826 pKa = 4.59 DD827 pKa = 4.02 IVNTGTISASDD838 pKa = 3.19 TGIAVEE844 pKa = 3.98 NSQVNGNILNEE855 pKa = 3.9 GSINGDD861 pKa = 3.2 YY862 pKa = 10.69 QGIHH866 pKa = 6.42 INGKK870 pKa = 10.0 LSGDD874 pKa = 3.45 ILNTGTISSDD884 pKa = 2.77 QGAAIYY890 pKa = 9.89 TSFGVDD896 pKa = 2.51 HH897 pKa = 7.46 DD898 pKa = 4.82 GSVEE902 pKa = 4.11 NYY904 pKa = 10.43 GSISGGNDD912 pKa = 3.17 GEE914 pKa = 4.7 TVTAIDD920 pKa = 3.92 YY921 pKa = 10.6 RR922 pKa = 11.84 NAFAPLTLVQKK933 pKa = 10.7 AGQIIGNIWGSSEE946 pKa = 4.47 DD947 pKa = 3.97 SGDD950 pKa = 3.27 SAYY953 pKa = 9.79 IDD955 pKa = 3.84 GGNITGDD962 pKa = 3.27 LHH964 pKa = 8.28 DD965 pKa = 4.48 IEE967 pKa = 6.1 GIYY970 pKa = 10.36 VDD972 pKa = 5.45 GVTTLDD978 pKa = 3.52 GNIYY982 pKa = 10.53 GADD985 pKa = 4.52 FINIEE990 pKa = 4.08 SSGNLTVSGEE1000 pKa = 3.86 ARR1002 pKa = 11.84 SFEE1005 pKa = 4.6 GYY1007 pKa = 10.78 LNLYY1011 pKa = 9.88 GGALSLNISNDD1022 pKa = 3.65 TKK1024 pKa = 10.89 PEE1026 pKa = 3.64 EE1027 pKa = 4.4 AMLSTEE1033 pKa = 3.93 GEE1035 pKa = 4.33 IYY1037 pKa = 10.62 LSSDD1041 pKa = 2.55 SRR1043 pKa = 11.84 LIITASQNLEE1053 pKa = 4.19 VNLDD1057 pKa = 3.73 PVDD1060 pKa = 3.93 YY1061 pKa = 10.64 LALSGSALYY1070 pKa = 10.95 NNGVTVEE1077 pKa = 4.27 SGSAIITAEE1086 pKa = 3.93 LAEE1089 pKa = 4.23 VTEE1092 pKa = 5.19 DD1093 pKa = 3.59 SLSVTVATNDD1103 pKa = 3.12 FGQVIEE1109 pKa = 4.57 SVGATGNEE1117 pKa = 4.0 VEE1119 pKa = 5.31 VIDD1122 pKa = 6.23 AYY1124 pKa = 8.54 MQEE1127 pKa = 4.71 YY1128 pKa = 9.22 IASSGGEE1135 pKa = 4.09 CNAICQAIEE1144 pKa = 3.77 QAEE1147 pKa = 4.29 TAEE1150 pKa = 4.08 EE1151 pKa = 3.73 LAAIAEE1157 pKa = 4.21 EE1158 pKa = 4.2 MVPDD1162 pKa = 3.66 SSGVVGASQAAQSEE1176 pKa = 4.62 TLGAVFKK1183 pKa = 10.61 RR1184 pKa = 11.84 IAGFRR1189 pKa = 11.84 SSVSGVSSGDD1199 pKa = 3.47 NIEE1202 pKa = 5.01 PGSLWMQALASDD1214 pKa = 4.64 GDD1216 pKa = 3.7 QDD1218 pKa = 3.71 AVQYY1222 pKa = 10.24 QGNDD1226 pKa = 2.89 FDD1228 pKa = 4.72 GYY1230 pKa = 10.07 SYY1232 pKa = 9.64 RR1233 pKa = 11.84 TRR1235 pKa = 11.84 GFTIGADD1242 pKa = 3.39 ADD1244 pKa = 4.05 LSDD1247 pKa = 4.05 TLTAGLAVTYY1257 pKa = 10.05 GVTKK1261 pKa = 10.7 SDD1263 pKa = 3.65 VEE1265 pKa = 4.44 GSANRR1270 pKa = 11.84 SEE1272 pKa = 4.13 TDD1274 pKa = 3.02 SYY1276 pKa = 11.84 LLTAYY1281 pKa = 10.0 GSWRR1285 pKa = 11.84 QQDD1288 pKa = 3.7 YY1289 pKa = 11.3 FMDD1292 pKa = 3.59 SSIAFGVSRR1301 pKa = 11.84 SDD1303 pKa = 3.25 IEE1305 pKa = 4.36 QYY1307 pKa = 8.73 TANVKK1312 pKa = 8.7 STADD1316 pKa = 3.07 SDD1318 pKa = 4.08 AAQVALRR1325 pKa = 11.84 LVTGRR1330 pKa = 11.84 TFAFNDD1336 pKa = 2.95 NDD1338 pKa = 4.67 TIVEE1342 pKa = 4.06 PQVAFNYY1349 pKa = 10.41 SRR1351 pKa = 11.84 VDD1353 pKa = 3.42 TNNYY1357 pKa = 7.54 TLQPLNMEE1365 pKa = 4.43 VLDD1368 pKa = 3.85 QRR1370 pKa = 11.84 IEE1372 pKa = 4.24 TVEE1375 pKa = 3.91 LGAGLRR1381 pKa = 11.84 AMTAIDD1387 pKa = 3.78 TKK1389 pKa = 10.68 RR1390 pKa = 11.84 GLLIPEE1396 pKa = 4.18 VNLMAWHH1403 pKa = 7.35 DD1404 pKa = 3.96 FAADD1408 pKa = 3.82 RR1409 pKa = 11.84 GDD1411 pKa = 3.26 TAARR1415 pKa = 11.84 FLTGAGNSFVTTGAKK1430 pKa = 9.88 PEE1432 pKa = 4.0 QTNYY1436 pKa = 9.93 QAGLGVQYY1444 pKa = 10.35 WLNNNVSLSANYY1456 pKa = 9.44 DD1457 pKa = 3.77 RR1458 pKa = 11.84 NWNSNFSADD1467 pKa = 2.9 TWLANIRR1474 pKa = 11.84 YY1475 pKa = 9.42 DD1476 pKa = 3.56 FF1477 pKa = 4.6
Molecular weight: 153.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.478
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A081K941|A0A081K941_9GAMM N-acetyltransferase domain-containing protein OS=Endozoicomonas elysicola OX=305900 GN=GV64_07870 PE=4 SV=1
MM1 pKa = 7.05 VLSGMFLVIMLLHH14 pKa = 6.56 LMVAMLIVFVSLFMGLMRR32 pKa = 11.84 VFMPLWVATVLGVRR46 pKa = 11.84 MAFFMLTIFQMITVVFIFNLRR67 pKa = 11.84 VILGRR72 pKa = 11.84 ALL74 pKa = 3.39
Molecular weight: 8.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 10.701
IPC_protein 12.31
Toseland 12.471
ProMoST 12.983
Dawson 12.471
Bjellqvist 12.471
Wikipedia 12.954
Rodwell 11.974
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.471
DTASelect 12.471
Thurlkill 12.471
EMBOSS 12.983
Sillero 12.471
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4147
0
4147
1412327
43
7482
340.6
37.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.196 ± 0.036
1.118 ± 0.017
5.66 ± 0.061
6.396 ± 0.037
3.972 ± 0.029
7.3 ± 0.072
2.272 ± 0.02
6.187 ± 0.029
4.979 ± 0.047
10.259 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.673 ± 0.024
4.124 ± 0.041
4.321 ± 0.036
4.316 ± 0.029
4.94 ± 0.041
7.016 ± 0.04
5.412 ± 0.059
6.682 ± 0.034
1.232 ± 0.015
2.944 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here