Legionella londiniensis
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0VRV5|A0A0W0VRV5_9GAMM CTP synthase OS=Legionella londiniensis OX=45068 GN=pyrG PE=3 SV=1
MM1 pKa = 7.31 AAIVSKK7 pKa = 10.92 AVFFALPKK15 pKa = 9.27 KK16 pKa = 8.69 TATATPLNSQNIADD30 pKa = 5.54 FIAGGYY36 pKa = 8.2 WLFPSTLNDD45 pKa = 3.21 QIYY48 pKa = 9.43 YY49 pKa = 10.46 QIFKK53 pKa = 10.97 DD54 pKa = 4.6 FALDD58 pKa = 3.58 PNGRR62 pKa = 11.84 YY63 pKa = 9.41 SLEE66 pKa = 4.41 LGDD69 pKa = 5.17 GVILLSEE76 pKa = 4.25 KK77 pKa = 11.03 NIDD80 pKa = 3.77 LSLILPPTAAGEE92 pKa = 4.1 DD93 pKa = 3.73 ANPILSYY100 pKa = 11.52 VNGSEE105 pKa = 4.22 VSSEE109 pKa = 3.93 LVFIEE114 pKa = 5.35 FINTLGQVEE123 pKa = 4.6 VGFDD127 pKa = 3.22 TRR129 pKa = 11.84 ASFKK133 pKa = 10.6 PFAYY137 pKa = 10.28 DD138 pKa = 3.96 LVPEE142 pKa = 4.29 EE143 pKa = 4.23 LTTYY147 pKa = 11.03 ALRR150 pKa = 11.84 FAFPDD155 pKa = 5.01 GIISSPSLSVPTIPSAPLIHH175 pKa = 6.74 LAADD179 pKa = 3.65 TGISNTDD186 pKa = 4.42 FITNNGSIIVSGLNPNATWQYY207 pKa = 10.99 SVNNGPWITGNGNSFVLPEE226 pKa = 4.09 GEE228 pKa = 4.29 YY229 pKa = 10.8 KK230 pKa = 10.63 EE231 pKa = 4.67 GAVKK235 pKa = 10.22 VRR237 pKa = 11.84 QINSEE242 pKa = 4.16 GNISNTSSLKK252 pKa = 9.02 PTVIDD257 pKa = 3.52 TTHH260 pKa = 6.88 PEE262 pKa = 3.4 INIEE266 pKa = 4.17 VNQTTEE272 pKa = 3.96 DD273 pKa = 3.65 NIVNIRR279 pKa = 11.84 EE280 pKa = 4.09 AGRR283 pKa = 11.84 DD284 pKa = 3.18 ITITGTVAGEE294 pKa = 3.99 FNEE297 pKa = 4.28 GDD299 pKa = 3.76 TVTLTVNGKK308 pKa = 8.63 EE309 pKa = 4.03 FQGAVNAEE317 pKa = 4.18 GLFSIAVPGRR327 pKa = 11.84 DD328 pKa = 3.15 LAADD332 pKa = 3.61 ANLTIEE338 pKa = 4.45 ASIATEE344 pKa = 3.68 DD345 pKa = 3.56 RR346 pKa = 11.84 AGNKK350 pKa = 8.05 ATASTDD356 pKa = 2.83 HH357 pKa = 6.79 TYY359 pKa = 10.86 SINYY363 pKa = 9.26 APEE366 pKa = 3.94 TMEE369 pKa = 5.06 DD370 pKa = 3.45 LAHH373 pKa = 6.23 VFEE376 pKa = 6.37 GDD378 pKa = 3.78 DD379 pKa = 4.19 LIGSNVLSNDD389 pKa = 3.08 TDD391 pKa = 3.81 QNSSDD396 pKa = 3.55 ILSVSLFSANSNGSGAEE413 pKa = 3.94 TANGSNTITTLLGGTVTMNADD434 pKa = 3.01 GTYY437 pKa = 9.5 TYY439 pKa = 10.8 SAPKK443 pKa = 9.96 SVNNINGADD452 pKa = 3.74 VVDD455 pKa = 3.78 SFYY458 pKa = 11.63 YY459 pKa = 10.15 KK460 pKa = 10.82 ASDD463 pKa = 4.35 GYY465 pKa = 11.47 NEE467 pKa = 4.32 SDD469 pKa = 3.1 WTKK472 pKa = 9.86 VTINIQDD479 pKa = 3.65 TSPTANPNEE488 pKa = 4.06 ATVAYY493 pKa = 9.33 QGSVSGNVTTNDD505 pKa = 4.03 LFGQDD510 pKa = 3.32 TAQLQSVTYY519 pKa = 10.26 NHH521 pKa = 6.16 ITYY524 pKa = 9.94 DD525 pKa = 3.39 QFVNGQLTINAANGVLTIHH544 pKa = 6.81 QDD546 pKa = 2.94 GTYY549 pKa = 9.3 TYY551 pKa = 10.49 QSNATLPRR559 pKa = 11.84 AEE561 pKa = 4.08 ITVGGSGGLSSWDD574 pKa = 3.03 GVTLHH579 pKa = 6.49 GFYY582 pKa = 10.67 LGATEE587 pKa = 4.44 PTPNSIGYY595 pKa = 8.7 RR596 pKa = 11.84 EE597 pKa = 4.38 HH598 pKa = 6.47 YY599 pKa = 10.01 GLYY602 pKa = 9.56 INSPGSVDD610 pKa = 3.22 NSRR613 pKa = 11.84 EE614 pKa = 3.6 IDD616 pKa = 3.42 SRR618 pKa = 11.84 AVGDD622 pKa = 3.65 TFKK625 pKa = 10.7 TEE627 pKa = 4.23 KK628 pKa = 10.32 IVVEE632 pKa = 3.91 FDD634 pKa = 3.73 VNVTEE639 pKa = 4.38 VTVGARR645 pKa = 11.84 DD646 pKa = 3.85 VQNHH650 pKa = 6.67 DD651 pKa = 4.07 SIHH654 pKa = 6.15 WIAYY658 pKa = 9.39 DD659 pKa = 3.64 EE660 pKa = 4.83 NGDD663 pKa = 3.81 EE664 pKa = 4.25 VATGIFTGEE673 pKa = 3.63 NSAPGNDD680 pKa = 3.21 HH681 pKa = 7.06 TYY683 pKa = 10.27 TFTITASEE691 pKa = 4.24 AFRR694 pKa = 11.84 SVAFMGHH701 pKa = 7.12 DD702 pKa = 3.76 GNDD705 pKa = 4.47 DD706 pKa = 3.52 FTLWNIKK713 pKa = 9.46 YY714 pKa = 9.93 IPAEE718 pKa = 4.22 ANQDD722 pKa = 3.48 VPPDD726 pKa = 3.29 VFTYY730 pKa = 10.97 VLVDD734 pKa = 3.62 ADD736 pKa = 4.0 GSTSQEE742 pKa = 3.75 TTLTITHH749 pKa = 7.53 DD750 pKa = 3.71 STLGTTAVSDD760 pKa = 3.64 EE761 pKa = 4.45 SSVFEE766 pKa = 4.23 SGLPQGTEE774 pKa = 3.53 AGIASIVASGNLLANDD790 pKa = 3.81 EE791 pKa = 4.48 NVTPDD796 pKa = 3.2 AAINEE801 pKa = 4.16 ISFNGNTFAPVDD813 pKa = 3.81 GVITIDD819 pKa = 3.56 TPLGILTVYY828 pKa = 10.38 TEE830 pKa = 4.89 DD831 pKa = 5.07 SVDD834 pKa = 3.3 GHH836 pKa = 6.29 NKK838 pKa = 9.74 GDD840 pKa = 3.95 YY841 pKa = 10.48 EE842 pKa = 4.25 YY843 pKa = 10.55 TLQNASNEE851 pKa = 3.93 GDD853 pKa = 3.59 HH854 pKa = 6.93 VSEE857 pKa = 4.35 AFTYY861 pKa = 10.42 QLINSSTGNASSSALTINIADD882 pKa = 4.29 DD883 pKa = 3.77 APRR886 pKa = 11.84 GQDD889 pKa = 2.51 IHH891 pKa = 8.24 ASLNKK896 pKa = 9.82 EE897 pKa = 3.91 GSALTINMTLVIDD910 pKa = 4.11 VSGSMNEE917 pKa = 3.73 IDD919 pKa = 3.94 SGEE922 pKa = 4.13 TASRR926 pKa = 11.84 LEE928 pKa = 3.93 LAKK931 pKa = 10.59 QSLEE935 pKa = 3.81 EE936 pKa = 4.51 LIRR939 pKa = 11.84 TGDD942 pKa = 3.31 EE943 pKa = 3.88 AGTINIKK950 pKa = 10.33 IIAFSTMAANSGWFVNNIEE969 pKa = 4.69 AAIDD973 pKa = 3.81 YY974 pKa = 10.83 LNLLQANGYY983 pKa = 7.36 TYY985 pKa = 11.16 YY986 pKa = 11.19 DD987 pKa = 3.32 NALQEE992 pKa = 4.68 VISSNGAPEE1001 pKa = 4.23 PSADD1005 pKa = 3.2 QHH1007 pKa = 6.42 LLYY1010 pKa = 10.28 FISDD1014 pKa = 4.19 GEE1016 pKa = 4.25 PTNNHH1021 pKa = 6.45 SIANGNLTYY1030 pKa = 10.72 NGYY1033 pKa = 10.47 EE1034 pKa = 4.0 DD1035 pKa = 3.83 TEE1037 pKa = 4.02 AWEE1040 pKa = 4.04 QFITDD1045 pKa = 3.63 SSIDD1049 pKa = 3.23 KK1050 pKa = 10.75 AFAVGFAGARR1060 pKa = 11.84 SSALEE1065 pKa = 3.71 PVAYY1069 pKa = 9.66 PNGEE1073 pKa = 4.23 DD1074 pKa = 3.34 GGEE1077 pKa = 4.05 VIIIDD1082 pKa = 4.18 SASDD1086 pKa = 3.43 LADD1089 pKa = 3.54 ALINSLVDD1097 pKa = 3.3 HH1098 pKa = 6.26 VQTGTVINTEE1108 pKa = 3.95 NASAEE1113 pKa = 4.1 GFIIGADD1120 pKa = 3.53 DD1121 pKa = 4.41 AGSHH1125 pKa = 5.97 IQSIMINHH1133 pKa = 5.59 VVYY1136 pKa = 10.09 EE1137 pKa = 4.34 FNPAEE1142 pKa = 4.09 PEE1144 pKa = 3.89 IEE1146 pKa = 4.1 VATGIKK1152 pKa = 10.31 GATLTFNFLTGTYY1165 pKa = 10.02 QYY1167 pKa = 10.44 TVDD1170 pKa = 3.82 SNEE1173 pKa = 4.04 EE1174 pKa = 3.72 IIAAEE1179 pKa = 4.2 TFPITLVDD1187 pKa = 3.51 GDD1189 pKa = 4.1 GDD1191 pKa = 3.72 EE1192 pKa = 4.4 KK1193 pKa = 10.62 TIHH1196 pKa = 6.26 LTVQVNYY1203 pKa = 9.15 EE1204 pKa = 3.82 ASIDD1208 pKa = 3.84 ANRR1211 pKa = 11.84 DD1212 pKa = 3.69 TIITNLTEE1220 pKa = 4.11 GSLNFDD1226 pKa = 4.37 ASALLNNDD1234 pKa = 3.19 AGITNDD1240 pKa = 3.49 VLISQVNNPKK1250 pKa = 10.68 GGTVSLNNNMIQFNLNAQYY1269 pKa = 10.9 SANDD1273 pKa = 3.58 FNNTQYY1279 pKa = 11.88 AKK1281 pKa = 10.17 IVNEE1285 pKa = 4.19 SEE1287 pKa = 4.18 NNNTKK1292 pKa = 10.76 LNADD1296 pKa = 4.03 EE1297 pKa = 4.41 ISRR1300 pKa = 11.84 GSFKK1304 pKa = 11.27 SNGLNNYY1311 pKa = 9.68 SMTYY1315 pKa = 9.95 RR1316 pKa = 11.84 GAVSSNDD1323 pKa = 3.34 ADD1325 pKa = 3.19 WFMLSLAAGEE1335 pKa = 4.46 LVHH1338 pKa = 7.67 IDD1340 pKa = 4.47 LNNTAYY1346 pKa = 10.95 NIDD1349 pKa = 3.19 IFAYY1353 pKa = 10.26 DD1354 pKa = 3.75 DD1355 pKa = 3.91 NGNQLTQIFDD1365 pKa = 3.88 YY1366 pKa = 10.48 TFSSYY1371 pKa = 11.22 YY1372 pKa = 8.26 GTYY1375 pKa = 10.66 GITNGLFAVQEE1386 pKa = 3.9 EE1387 pKa = 4.77 GNYY1390 pKa = 9.49 YY1391 pKa = 10.25 FQVAKK1396 pKa = 10.96 NSGSSLNYY1404 pKa = 8.84 EE1405 pKa = 4.08 ANITIDD1411 pKa = 3.44 TSNAQYY1417 pKa = 10.83 QGFDD1421 pKa = 3.44 YY1422 pKa = 10.74 TVNDD1426 pKa = 3.83 HH1427 pKa = 6.32 NAVEE1431 pKa = 4.53 ISAPVNIIVQEE1442 pKa = 4.18 GNEE1445 pKa = 4.15 LTGSEE1450 pKa = 4.21 ASEE1453 pKa = 3.78 ILIGSDD1459 pKa = 3.24 GDD1461 pKa = 4.37 DD1462 pKa = 4.3 MINADD1467 pKa = 4.36 GGDD1470 pKa = 3.66 DD1471 pKa = 3.7 VLFAGLGDD1479 pKa = 5.38 DD1480 pKa = 4.26 ILHH1483 pKa = 6.64 GGDD1486 pKa = 4.33 GNDD1489 pKa = 3.32 TLFGGEE1495 pKa = 4.77 GNDD1498 pKa = 3.47 TMTGGDD1504 pKa = 3.77 GQNTFVWKK1512 pKa = 10.68 AGDD1515 pKa = 3.76 DD1516 pKa = 3.66 AGGSIDD1522 pKa = 4.34 TITDD1526 pKa = 4.24 FKK1528 pKa = 11.02 LGQNGDD1534 pKa = 3.88 VLDD1537 pKa = 5.03 LSDD1540 pKa = 4.92 LLVGEE1545 pKa = 4.78 SNTAEE1550 pKa = 4.25 SLDD1553 pKa = 3.59 AYY1555 pKa = 11.39 LNISYY1560 pKa = 10.89 DD1561 pKa = 3.61 NASNTSTVSVNPDD1574 pKa = 2.68 GSASFSATQAIVLSGVDD1591 pKa = 3.59 LTAGGTLQTDD1601 pKa = 3.55 QAILDD1606 pKa = 3.75 VLINNGNIVTDD1617 pKa = 4.25 GGG1619 pKa = 3.73
Molecular weight: 172.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.91
Patrickios 0.935
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A0W0VT33|A0A0W0VT33_9GAMM 3-hydroxymyristoyl-ACP dehydratase OS=Legionella londiniensis OX=45068 GN=fabA PE=4 SV=1
MM1 pKa = 7.55 ALIKK5 pKa = 10.53 SKK7 pKa = 9.07 PTSPGKK13 pKa = 10.09 RR14 pKa = 11.84 GEE16 pKa = 3.61 IRR18 pKa = 11.84 VRR20 pKa = 11.84 HH21 pKa = 5.9 DD22 pKa = 3.38 VYY24 pKa = 11.16 KK25 pKa = 10.86 GDD27 pKa = 4.54 PFPGLISTVKK37 pKa = 9.51 KK38 pKa = 9.15 TGGRR42 pKa = 11.84 NNQGRR47 pKa = 11.84 ITVRR51 pKa = 11.84 HH52 pKa = 5.83 IGGGTRR58 pKa = 11.84 VQYY61 pKa = 10.79 RR62 pKa = 11.84 HH63 pKa = 6.6 IDD65 pKa = 3.68 FKK67 pKa = 11.19 RR68 pKa = 11.84 NKK70 pKa = 9.81 DD71 pKa = 3.49 GIEE74 pKa = 3.79 ARR76 pKa = 11.84 VEE78 pKa = 4.11 RR79 pKa = 11.84 IEE81 pKa = 3.97 YY82 pKa = 10.37 DD83 pKa = 3.49 PNRR86 pKa = 11.84 SALIALIVYY95 pKa = 10.06 KK96 pKa = 10.6 DD97 pKa = 3.22 GEE99 pKa = 4.23 RR100 pKa = 11.84 RR101 pKa = 11.84 YY102 pKa = 10.08 IIAPAGLALNDD113 pKa = 4.01 TVVSGDD119 pKa = 4.09 DD120 pKa = 3.7 APISTGNALPLKK132 pKa = 8.99 NIPVGTTVHH141 pKa = 6.24 CVEE144 pKa = 5.21 LKK146 pKa = 10.19 PGCGAQLLRR155 pKa = 11.84 SAGCSGQIVAKK166 pKa = 9.68 EE167 pKa = 3.95 GAYY170 pKa = 8.78 STLKK174 pKa = 10.45 LRR176 pKa = 11.84 SGEE179 pKa = 3.95 MRR181 pKa = 11.84 KK182 pKa = 9.5 VLSACRR188 pKa = 11.84 ATVGEE193 pKa = 4.46 VSNSEE198 pKa = 4.06 HH199 pKa = 6.74 NLRR202 pKa = 11.84 ALGKK206 pKa = 10.04 AGASRR211 pKa = 11.84 WRR213 pKa = 11.84 GKK215 pKa = 10.27 RR216 pKa = 11.84 PTVRR220 pKa = 11.84 GVAMNPVDD228 pKa = 3.85 HH229 pKa = 6.82 PHH231 pKa = 6.77 GGGEE235 pKa = 4.38 GKK237 pKa = 9.02 TSGGRR242 pKa = 11.84 HH243 pKa = 5.83 PVTPWGIPTKK253 pKa = 10.33 GYY255 pKa = 7.47 KK256 pKa = 7.77 TRR258 pKa = 11.84 RR259 pKa = 11.84 NKK261 pKa = 9.32 RR262 pKa = 11.84 TSKK265 pKa = 10.29 YY266 pKa = 9.53 IVRR269 pKa = 11.84 GRR271 pKa = 11.84 KK272 pKa = 8.88 NKK274 pKa = 10.12
Molecular weight: 29.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.794
IPC_protein 10.511
Toseland 10.847
ProMoST 10.526
Dawson 10.935
Bjellqvist 10.613
Wikipedia 11.125
Rodwell 11.242
Grimsley 10.965
Solomon 11.038
Lehninger 11.008
Nozaki 10.833
DTASelect 10.613
Thurlkill 10.833
EMBOSS 11.257
Sillero 10.862
Patrickios 10.95
IPC_peptide 11.052
IPC2_peptide 9.516
IPC2.peptide.svr19 8.593
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2172
0
2172
722513
51
3879
332.6
37.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.372 ± 0.056
1.188 ± 0.018
4.7 ± 0.04
6.336 ± 0.054
4.471 ± 0.038
6.208 ± 0.044
2.595 ± 0.026
7.254 ± 0.047
6.19 ± 0.058
11.267 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.021
4.372 ± 0.041
4.137 ± 0.034
4.593 ± 0.04
4.794 ± 0.039
6.081 ± 0.031
4.715 ± 0.033
5.787 ± 0.048
1.143 ± 0.02
3.393 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here