Mastomys natalensis polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5RUP2|E5RUP2_9POLY Minor capsid protein OS=Mastomys natalensis polyomavirus 1 OX=1891768 GN=VP3 PE=3 SV=1
MM1 pKa = 7.44GAFLTVLAEE10 pKa = 4.16VFEE13 pKa = 4.57LASVTGLSAEE23 pKa = 4.43SIISGEE29 pKa = 4.08AFTTAEE35 pKa = 4.49LLQSHH40 pKa = 7.17IANLVTYY47 pKa = 10.69GEE49 pKa = 4.21LTEE52 pKa = 5.18AEE54 pKa = 4.11ALAAAEE60 pKa = 4.43VSTEE64 pKa = 4.16AYY66 pKa = 9.86SALQTLTPNFPQVLAALAASEE87 pKa = 4.01ITATGSITVGAAVAAALYY105 pKa = 8.45PYY107 pKa = 9.68SYY109 pKa = 10.97EE110 pKa = 4.16FSTPLANLNQNMALQLWFPDD130 pKa = 2.57VDD132 pKa = 4.2YY133 pKa = 11.23NFPGLLPFVRR143 pKa = 11.84FVNYY147 pKa = 9.61IDD149 pKa = 3.84PARR152 pKa = 11.84WASDD156 pKa = 3.41LYY158 pKa = 10.78HH159 pKa = 6.97AVGRR163 pKa = 11.84YY164 pKa = 7.03FWQSVEE170 pKa = 4.25RR171 pKa = 11.84EE172 pKa = 3.9ASRR175 pKa = 11.84QIGDD179 pKa = 3.95LSRR182 pKa = 11.84DD183 pKa = 3.11LALRR187 pKa = 11.84TTQTVGEE194 pKa = 4.1TLARR198 pKa = 11.84YY199 pKa = 9.2FEE201 pKa = 4.06NARR204 pKa = 11.84WAVTNLPSLNLYY216 pKa = 10.41SGLEE220 pKa = 4.04TYY222 pKa = 10.24YY223 pKa = 11.39SKK225 pKa = 11.24LSPLNPIRR233 pKa = 11.84ARR235 pKa = 11.84QLARR239 pKa = 11.84NLGEE243 pKa = 4.19EE244 pKa = 3.83EE245 pKa = 4.31PYY247 pKa = 10.55RR248 pKa = 11.84YY249 pKa = 10.13DD250 pKa = 4.25RR251 pKa = 11.84YY252 pKa = 11.23DD253 pKa = 3.52GAQSNQKK260 pKa = 9.28SAEE263 pKa = 4.04YY264 pKa = 10.04VEE266 pKa = 5.84KK267 pKa = 10.82YY268 pKa = 10.23DD269 pKa = 4.16SPGGAHH275 pKa = 6.11QRR277 pKa = 11.84HH278 pKa = 5.59TPDD281 pKa = 2.5WMLPLILGLYY291 pKa = 10.37GDD293 pKa = 5.15ISPSWGEE300 pKa = 3.83TIKK303 pKa = 10.79EE304 pKa = 3.95IEE306 pKa = 4.3EE307 pKa = 4.3EE308 pKa = 3.84EE309 pKa = 4.48DD310 pKa = 3.74GPKK313 pKa = 10.16KK314 pKa = 10.21KK315 pKa = 10.28RR316 pKa = 11.84PRR318 pKa = 11.84KK319 pKa = 9.78AGWKK323 pKa = 9.73ASRR326 pKa = 11.84STKK329 pKa = 10.45ANHH332 pKa = 6.14

Molecular weight: 36.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5RUP1|E5RUP1_9POLY Minor capsid protein OS=Mastomys natalensis polyomavirus 1 OX=1891768 GN=VP2 PE=3 SV=1
MM1 pKa = 7.36IQVLWQTLKK10 pKa = 10.76CLLRR14 pKa = 11.84VFSISHH20 pKa = 6.69ASAAVLGDD28 pKa = 3.15IMCILEE34 pKa = 4.35EE35 pKa = 4.32FYY37 pKa = 11.48KK38 pKa = 10.61LAKK41 pKa = 8.58EE42 pKa = 4.28HH43 pKa = 6.16KK44 pKa = 7.9TVLNKK49 pKa = 9.35VEE51 pKa = 4.88CGWEE55 pKa = 3.74LARR58 pKa = 11.84FKK60 pKa = 11.16QKK62 pKa = 10.34INYY65 pKa = 9.43YY66 pKa = 7.29MDD68 pKa = 3.54KK69 pKa = 11.03VKK71 pKa = 10.12ATLKK75 pKa = 10.47HH76 pKa = 5.78CGKK79 pKa = 9.24VQVMYY84 pKa = 10.13FSKK87 pKa = 10.57QGTDD91 pKa = 3.08SVDD94 pKa = 3.3RR95 pKa = 11.84NLACVPEE102 pKa = 4.54LGKK105 pKa = 9.58GTLALSLGHH114 pKa = 6.01SEE116 pKa = 4.39QEE118 pKa = 4.29RR119 pKa = 11.84EE120 pKa = 4.03EE121 pKa = 4.25TACDD125 pKa = 3.65CLACQDD131 pKa = 3.96MRR133 pKa = 11.84QQLNLAEE140 pKa = 4.57HH141 pKa = 6.27LRR143 pKa = 11.84SLTEE147 pKa = 3.92EE148 pKa = 4.05LQKK151 pKa = 11.58LLMM154 pKa = 4.97

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1876

154

653

312.7

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.716 ± 1.365

2.239 ± 0.728

5.171 ± 0.398

7.463 ± 0.308

3.945 ± 0.386

6.023 ± 0.907

1.866 ± 0.276

4.318 ± 0.382

6.503 ± 0.97

11.407 ± 0.748

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.426

4.744 ± 0.428

5.277 ± 0.705

4.584 ± 0.304

4.904 ± 0.607

5.171 ± 0.698

5.97 ± 0.405

5.384 ± 0.548

1.599 ± 0.384

4.264 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski