Caulobacter phage CcrBL10
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 319 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385EC31|A0A385EC31_9CAUD Uncharacterized protein OS=Caulobacter phage CcrBL10 OX=2283269 GN=CcrBL10_gp084 PE=4 SV=1
MM1 pKa = 7.53 IPAEE5 pKa = 3.97 VLNPRR10 pKa = 11.84 EE11 pKa = 3.92 HH12 pKa = 5.88 WTPVATRR19 pKa = 11.84 DD20 pKa = 3.57 EE21 pKa = 4.56 AEE23 pKa = 4.72 SIATACNASDD33 pKa = 3.76 DD34 pKa = 3.75 VGRR37 pKa = 11.84 YY38 pKa = 7.34 YY39 pKa = 11.0 VEE41 pKa = 4.0 PKK43 pKa = 10.25 GAAFAVAYY51 pKa = 9.85 DD52 pKa = 4.12 DD53 pKa = 4.02 EE54 pKa = 6.49 SGAFVAYY61 pKa = 10.4 LL62 pKa = 3.45
Molecular weight: 6.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.919
IPC2_protein 4.24
IPC_protein 4.088
Toseland 3.91
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.923
Grimsley 3.834
Solomon 4.037
Lehninger 3.986
Nozaki 4.177
DTASelect 4.329
Thurlkill 3.961
EMBOSS 3.973
Sillero 4.202
Patrickios 1.952
IPC_peptide 4.037
IPC2_peptide 4.19
IPC2.peptide.svr19 4.09
Protein with the highest isoelectric point:
>tr|A0A385EBM9|A0A385EBM9_9CAUD Uncharacterized protein OS=Caulobacter phage CcrBL10 OX=2283269 GN=CcrBL10_gp068 PE=4 SV=1
MM1 pKa = 6.98 QNVPVGHH8 pKa = 7.04 RR9 pKa = 11.84 FTRR12 pKa = 11.84 YY13 pKa = 7.61 GQTYY17 pKa = 7.29 VVRR20 pKa = 11.84 PGLRR24 pKa = 11.84 YY25 pKa = 10.04 VPEE28 pKa = 4.16 AQAADD33 pKa = 3.43 ASRR36 pKa = 11.84 RR37 pKa = 11.84 GWTLQNSDD45 pKa = 4.32 LKK47 pKa = 11.27 ALLGWTPKK55 pKa = 10.47 LPRR58 pKa = 11.84 DD59 pKa = 3.33 AVAPVMVGNVSVYY72 pKa = 10.36 VLSAAEE78 pKa = 3.61 AAARR82 pKa = 11.84 KK83 pKa = 8.44 PKK85 pKa = 10.22 NNRR88 pKa = 11.84 PHH90 pKa = 7.19 RR91 pKa = 11.84 VTAVCPGCAAHH102 pKa = 6.65 VPAGRR107 pKa = 11.84 IGQHH111 pKa = 5.04 KK112 pKa = 10.09 CKK114 pKa = 10.65 AA115 pKa = 3.48
Molecular weight: 12.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.838
IPC_protein 10.643
Toseland 10.687
ProMoST 10.409
Dawson 10.804
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.038
Grimsley 10.862
Solomon 10.906
Lehninger 10.877
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 10.789
IPC_peptide 10.906
IPC2_peptide 9.692
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
319
0
319
62993
41
2384
197.5
21.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.05 ± 0.196
0.916 ± 0.059
6.744 ± 0.164
6.134 ± 0.203
3.773 ± 0.101
8.487 ± 0.46
2.143 ± 0.103
4.726 ± 0.091
4.699 ± 0.189
8.095 ± 0.145
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.104
3.002 ± 0.121
5.088 ± 0.13
3.264 ± 0.104
6.528 ± 0.226
5.17 ± 0.189
6.155 ± 0.27
6.955 ± 0.126
1.646 ± 0.079
3.034 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here