Amino acid dipepetide frequency for Caulobacter phage CcrBL10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.398AlaAla: 12.398 ± 0.684
1.016AlaCys: 1.016 ± 0.125
6.826AlaAsp: 6.826 ± 0.36
6.779AlaGlu: 6.779 ± 0.356
4.223AlaPhe: 4.223 ± 0.283
9.525AlaGly: 9.525 ± 0.527
2.048AlaHis: 2.048 ± 0.185
5.207AlaIle: 5.207 ± 0.303
5.525AlaLys: 5.525 ± 0.441
10.493AlaLeu: 10.493 ± 0.53
3.477AlaMet: 3.477 ± 0.273
3.334AlaAsn: 3.334 ± 0.295
5.493AlaPro: 5.493 ± 0.26
4.397AlaGln: 4.397 ± 0.354
6.953AlaArg: 6.953 ± 0.391
5.302AlaSer: 5.302 ± 0.321
5.366AlaThr: 5.366 ± 0.485
6.874AlaVal: 6.874 ± 0.359
1.969AlaTrp: 1.969 ± 0.182
3.286AlaTyr: 3.286 ± 0.265
0.0AlaXaa: 0.0 ± 0.0
Cys
0.746CysAla: 0.746 ± 0.117
0.191CysCys: 0.191 ± 0.058
0.619CysAsp: 0.619 ± 0.107
0.778CysGlu: 0.778 ± 0.102
0.27CysPhe: 0.27 ± 0.068
0.857CysGly: 0.857 ± 0.137
0.286CysHis: 0.286 ± 0.069
0.302CysIle: 0.302 ± 0.079
0.365CysLys: 0.365 ± 0.069
0.714CysLeu: 0.714 ± 0.109
0.206CysMet: 0.206 ± 0.058
0.206CysAsn: 0.206 ± 0.062
0.413CysPro: 0.413 ± 0.083
0.302CysGln: 0.302 ± 0.065
0.921CysArg: 0.921 ± 0.131
0.46CysSer: 0.46 ± 0.09
0.46CysThr: 0.46 ± 0.081
0.556CysVal: 0.556 ± 0.104
0.127CysTrp: 0.127 ± 0.043
0.381CysTyr: 0.381 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
6.747AspAla: 6.747 ± 0.377
0.714AspCys: 0.714 ± 0.101
4.588AspAsp: 4.588 ± 0.317
4.778AspGlu: 4.778 ± 0.417
2.461AspPhe: 2.461 ± 0.196
6.683AspGly: 6.683 ± 0.364
1.476AspHis: 1.476 ± 0.178
3.048AspIle: 3.048 ± 0.194
2.715AspLys: 2.715 ± 0.237
6.874AspLeu: 6.874 ± 0.382
1.572AspMet: 1.572 ± 0.163
1.572AspAsn: 1.572 ± 0.147
3.953AspPro: 3.953 ± 0.254
1.762AspGln: 1.762 ± 0.187
3.905AspArg: 3.905 ± 0.308
2.223AspSer: 2.223 ± 0.179
3.556AspThr: 3.556 ± 0.238
5.112AspVal: 5.112 ± 0.292
1.27AspTrp: 1.27 ± 0.151
2.429AspTyr: 2.429 ± 0.205
0.0AspXaa: 0.0 ± 0.0
Glu
8.477GluAla: 8.477 ± 0.462
0.445GluCys: 0.445 ± 0.078
4.112GluAsp: 4.112 ± 0.396
3.905GluGlu: 3.905 ± 0.33
2.556GluPhe: 2.556 ± 0.182
3.937GluGly: 3.937 ± 0.245
1.54GluHis: 1.54 ± 0.187
3.604GluIle: 3.604 ± 0.261
2.794GluLys: 2.794 ± 0.239
3.54GluLeu: 3.54 ± 0.222
1.619GluMet: 1.619 ± 0.17
1.572GluAsn: 1.572 ± 0.2
2.921GluPro: 2.921 ± 0.267
2.445GluGln: 2.445 ± 0.231
4.89GluArg: 4.89 ± 0.411
2.365GluSer: 2.365 ± 0.162
3.826GluThr: 3.826 ± 0.271
4.667GluVal: 4.667 ± 0.277
0.857GluTrp: 0.857 ± 0.103
1.27GluTyr: 1.27 ± 0.162
0.0GluXaa: 0.0 ± 0.0
Phe
3.413PheAla: 3.413 ± 0.225
0.397PheCys: 0.397 ± 0.082
3.064PheAsp: 3.064 ± 0.244
2.635PheGlu: 2.635 ± 0.198
1.429PhePhe: 1.429 ± 0.161
3.0PheGly: 3.0 ± 0.222
1.08PheHis: 1.08 ± 0.155
1.413PheIle: 1.413 ± 0.14
2.096PheLys: 2.096 ± 0.17
2.953PheLeu: 2.953 ± 0.209
0.873PheMet: 0.873 ± 0.124
1.175PheAsn: 1.175 ± 0.144
1.619PhePro: 1.619 ± 0.158
1.207PheGln: 1.207 ± 0.145
2.048PheArg: 2.048 ± 0.176
2.143PheSer: 2.143 ± 0.217
2.429PheThr: 2.429 ± 0.213
2.619PheVal: 2.619 ± 0.21
0.699PheTrp: 0.699 ± 0.09
1.445PheTyr: 1.445 ± 0.166
0.0PheXaa: 0.0 ± 0.0
Gly
8.16GlyAla: 8.16 ± 0.66
0.794GlyCys: 0.794 ± 0.109
5.62GlyAsp: 5.62 ± 0.353
4.62GlyGlu: 4.62 ± 0.241
3.318GlyPhe: 3.318 ± 0.206
10.557GlyGly: 10.557 ± 2.021
1.603GlyHis: 1.603 ± 0.155
2.651GlyIle: 2.651 ± 0.216
4.048GlyLys: 4.048 ± 0.284
6.112GlyLeu: 6.112 ± 0.409
2.334GlyMet: 2.334 ± 0.206
2.27GlyAsn: 2.27 ± 0.243
3.064GlyPro: 3.064 ± 0.218
2.858GlyGln: 2.858 ± 0.222
5.302GlyArg: 5.302 ± 0.299
5.413GlySer: 5.413 ± 0.508
5.175GlyThr: 5.175 ± 0.676
6.033GlyVal: 6.033 ± 0.353
1.889GlyTrp: 1.889 ± 0.204
3.048GlyTyr: 3.048 ± 0.297
0.0GlyXaa: 0.0 ± 0.0
His
2.572HisAla: 2.572 ± 0.225
0.238HisCys: 0.238 ± 0.062
1.699HisAsp: 1.699 ± 0.164
1.318HisGlu: 1.318 ± 0.146
0.81HisPhe: 0.81 ± 0.124
1.905HisGly: 1.905 ± 0.203
0.587HisHis: 0.587 ± 0.103
0.921HisIle: 0.921 ± 0.125
0.714HisLys: 0.714 ± 0.13
1.746HisLeu: 1.746 ± 0.153
0.492HisMet: 0.492 ± 0.099
0.54HisAsn: 0.54 ± 0.091
1.318HisPro: 1.318 ± 0.16
0.699HisGln: 0.699 ± 0.101
1.476HisArg: 1.476 ± 0.163
0.937HisSer: 0.937 ± 0.136
0.841HisThr: 0.841 ± 0.135
1.524HisVal: 1.524 ± 0.187
0.413HisTrp: 0.413 ± 0.082
0.683HisTyr: 0.683 ± 0.093
0.0HisXaa: 0.0 ± 0.0
Ile
5.858IleAla: 5.858 ± 0.304
0.476IleCys: 0.476 ± 0.087
3.826IleAsp: 3.826 ± 0.305
3.762IleGlu: 3.762 ± 0.288
1.302IlePhe: 1.302 ± 0.141
3.842IleGly: 3.842 ± 0.299
1.032IleHis: 1.032 ± 0.146
1.857IleIle: 1.857 ± 0.199
2.318IleLys: 2.318 ± 0.213
3.334IleLeu: 3.334 ± 0.204
0.937IleMet: 0.937 ± 0.129
1.492IleAsn: 1.492 ± 0.171
2.127IlePro: 2.127 ± 0.165
1.476IleGln: 1.476 ± 0.164
2.461IleArg: 2.461 ± 0.242
2.413IleSer: 2.413 ± 0.175
3.223IleThr: 3.223 ± 0.352
3.699IleVal: 3.699 ± 0.226
0.651IleTrp: 0.651 ± 0.106
1.175IleTyr: 1.175 ± 0.139
0.0IleXaa: 0.0 ± 0.0
Lys
6.144LysAla: 6.144 ± 0.37
0.318LysCys: 0.318 ± 0.071
2.715LysAsp: 2.715 ± 0.247
2.0LysGlu: 2.0 ± 0.198
1.603LysPhe: 1.603 ± 0.152
3.572LysGly: 3.572 ± 0.281
0.953LysHis: 0.953 ± 0.122
2.254LysIle: 2.254 ± 0.165
2.477LysLys: 2.477 ± 0.233
4.175LysLeu: 4.175 ± 0.292
0.953LysMet: 0.953 ± 0.143
1.143LysAsn: 1.143 ± 0.13
2.556LysPro: 2.556 ± 0.237
1.254LysGln: 1.254 ± 0.132
3.683LysArg: 3.683 ± 0.277
2.254LysSer: 2.254 ± 0.186
3.54LysThr: 3.54 ± 0.268
3.699LysVal: 3.699 ± 0.244
0.651LysTrp: 0.651 ± 0.097
1.048LysTyr: 1.048 ± 0.135
0.0LysXaa: 0.0 ± 0.0
Leu
9.366LeuAla: 9.366 ± 0.524
0.841LeuCys: 0.841 ± 0.116
5.779LeuAsp: 5.779 ± 0.303
5.017LeuGlu: 5.017 ± 0.314
2.318LeuPhe: 2.318 ± 0.212
6.112LeuGly: 6.112 ± 0.332
1.762LeuHis: 1.762 ± 0.174
3.794LeuIle: 3.794 ± 0.213
4.159LeuLys: 4.159 ± 0.289
5.906LeuLeu: 5.906 ± 0.372
1.794LeuMet: 1.794 ± 0.193
2.794LeuAsn: 2.794 ± 0.251
4.207LeuPro: 4.207 ± 0.289
2.572LeuGln: 2.572 ± 0.239
5.54LeuArg: 5.54 ± 0.34
5.08LeuSer: 5.08 ± 0.403
5.604LeuThr: 5.604 ± 0.332
4.905LeuVal: 4.905 ± 0.306
1.143LeuTrp: 1.143 ± 0.115
2.254LeuTyr: 2.254 ± 0.225
0.0LeuXaa: 0.0 ± 0.0
Met
2.842MetAla: 2.842 ± 0.251
0.159MetCys: 0.159 ± 0.045
1.254MetAsp: 1.254 ± 0.151
1.111MetGlu: 1.111 ± 0.145
0.699MetPhe: 0.699 ± 0.108
1.508MetGly: 1.508 ± 0.195
0.349MetHis: 0.349 ± 0.077
1.365MetIle: 1.365 ± 0.169
1.381MetLys: 1.381 ± 0.143
1.683MetLeu: 1.683 ± 0.198
0.603MetMet: 0.603 ± 0.107
0.937MetAsn: 0.937 ± 0.117
1.381MetPro: 1.381 ± 0.165
0.778MetGln: 0.778 ± 0.127
1.746MetArg: 1.746 ± 0.171
1.857MetSer: 1.857 ± 0.172
2.223MetThr: 2.223 ± 0.21
1.365MetVal: 1.365 ± 0.139
0.286MetTrp: 0.286 ± 0.077
0.397MetTyr: 0.397 ± 0.088
0.0MetXaa: 0.0 ± 0.0
Asn
3.588AsnAla: 3.588 ± 0.252
0.286AsnCys: 0.286 ± 0.07
1.81AsnAsp: 1.81 ± 0.167
1.651AsnGlu: 1.651 ± 0.156
0.905AsnPhe: 0.905 ± 0.138
3.08AsnGly: 3.08 ± 0.341
0.556AsnHis: 0.556 ± 0.113
1.476AsnIle: 1.476 ± 0.156
1.032AsnLys: 1.032 ± 0.118
2.778AsnLeu: 2.778 ± 0.219
0.635AsnMet: 0.635 ± 0.115
1.191AsnAsn: 1.191 ± 0.176
1.953AsnPro: 1.953 ± 0.152
0.746AsnGln: 0.746 ± 0.103
1.619AsnArg: 1.619 ± 0.176
1.429AsnSer: 1.429 ± 0.147
1.969AsnThr: 1.969 ± 0.22
1.953AsnVal: 1.953 ± 0.192
0.365AsnTrp: 0.365 ± 0.068
1.0AsnTyr: 1.0 ± 0.146
0.0AsnXaa: 0.0 ± 0.0
Pro
5.493ProAla: 5.493 ± 0.303
0.397ProCys: 0.397 ± 0.083
3.588ProAsp: 3.588 ± 0.191
3.604ProGlu: 3.604 ± 0.281
1.905ProPhe: 1.905 ± 0.181
4.35ProGly: 4.35 ± 0.267
1.302ProHis: 1.302 ± 0.146
2.731ProIle: 2.731 ± 0.207
2.127ProLys: 2.127 ± 0.176
3.588ProLeu: 3.588 ± 0.258
1.111ProMet: 1.111 ± 0.14
1.857ProAsn: 1.857 ± 0.173
3.064ProPro: 3.064 ± 0.308
1.461ProGln: 1.461 ± 0.179
2.746ProArg: 2.746 ± 0.292
2.715ProSer: 2.715 ± 0.226
3.477ProThr: 3.477 ± 0.273
3.207ProVal: 3.207 ± 0.223
0.683ProTrp: 0.683 ± 0.101
1.476ProTyr: 1.476 ± 0.138
0.0ProXaa: 0.0 ± 0.0
Gln
4.477GlnAla: 4.477 ± 0.302
0.254GlnCys: 0.254 ± 0.069
2.0GlnAsp: 2.0 ± 0.201
2.0GlnGlu: 2.0 ± 0.187
1.159GlnPhe: 1.159 ± 0.139
2.492GlnGly: 2.492 ± 0.229
0.714GlnHis: 0.714 ± 0.11
1.889GlnIle: 1.889 ± 0.178
1.556GlnLys: 1.556 ± 0.175
1.715GlnLeu: 1.715 ± 0.22
0.699GlnMet: 0.699 ± 0.118
0.826GlnAsn: 0.826 ± 0.1
1.556GlnPro: 1.556 ± 0.148
1.08GlnGln: 1.08 ± 0.148
2.508GlnArg: 2.508 ± 0.19
1.619GlnSer: 1.619 ± 0.16
2.159GlnThr: 2.159 ± 0.204
2.556GlnVal: 2.556 ± 0.184
0.508GlnTrp: 0.508 ± 0.09
0.873GlnTyr: 0.873 ± 0.111
0.0GlnXaa: 0.0 ± 0.0
Arg
6.08ArgAla: 6.08 ± 0.357
0.54ArgCys: 0.54 ± 0.101
4.524ArgAsp: 4.524 ± 0.331
4.191ArgGlu: 4.191 ± 0.33
3.207ArgPhe: 3.207 ± 0.231
4.461ArgGly: 4.461 ± 0.257
1.365ArgHis: 1.365 ± 0.182
3.08ArgIle: 3.08 ± 0.199
3.159ArgLys: 3.159 ± 0.242
6.271ArgLeu: 6.271 ± 0.399
1.842ArgMet: 1.842 ± 0.174
1.857ArgAsn: 1.857 ± 0.165
2.985ArgPro: 2.985 ± 0.262
2.461ArgGln: 2.461 ± 0.213
5.175ArgArg: 5.175 ± 0.439
2.826ArgSer: 2.826 ± 0.282
3.0ArgThr: 3.0 ± 0.223
4.302ArgVal: 4.302 ± 0.254
1.365ArgTrp: 1.365 ± 0.148
2.588ArgTyr: 2.588 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
5.223SerAla: 5.223 ± 0.354
0.445SerCys: 0.445 ± 0.086
3.286SerAsp: 3.286 ± 0.227
2.778SerGlu: 2.778 ± 0.245
2.381SerPhe: 2.381 ± 0.185
5.175SerGly: 5.175 ± 0.604
0.921SerHis: 0.921 ± 0.121
2.731SerIle: 2.731 ± 0.2
2.413SerLys: 2.413 ± 0.206
4.112SerLeu: 4.112 ± 0.278
1.143SerMet: 1.143 ± 0.135
1.635SerAsn: 1.635 ± 0.194
2.381SerPro: 2.381 ± 0.184
1.683SerGln: 1.683 ± 0.207
3.112SerArg: 3.112 ± 0.247
2.889SerSer: 2.889 ± 0.263
3.413SerThr: 3.413 ± 0.299
3.366SerVal: 3.366 ± 0.234
0.889SerTrp: 0.889 ± 0.12
1.73SerTyr: 1.73 ± 0.19
0.0SerXaa: 0.0 ± 0.0
Thr
6.953ThrAla: 6.953 ± 0.469
0.54ThrCys: 0.54 ± 0.124
3.667ThrAsp: 3.667 ± 0.288
3.112ThrGlu: 3.112 ± 0.258
2.953ThrPhe: 2.953 ± 0.213
5.334ThrGly: 5.334 ± 0.583
1.27ThrHis: 1.27 ± 0.133
3.556ThrIle: 3.556 ± 0.426
2.461ThrLys: 2.461 ± 0.218
5.683ThrLeu: 5.683 ± 0.317
1.207ThrMet: 1.207 ± 0.15
1.826ThrAsn: 1.826 ± 0.198
4.159ThrPro: 4.159 ± 0.354
1.826ThrGln: 1.826 ± 0.147
3.223ThrArg: 3.223 ± 0.25
3.175ThrSer: 3.175 ± 0.3
3.334ThrThr: 3.334 ± 0.351
4.477ThrVal: 4.477 ± 0.399
0.857ThrTrp: 0.857 ± 0.109
1.937ThrTyr: 1.937 ± 0.216
0.0ThrXaa: 0.0 ± 0.0
Val
6.731ValAla: 6.731 ± 0.363
0.603ValCys: 0.603 ± 0.094
5.048ValAsp: 5.048 ± 0.293
4.572ValGlu: 4.572 ± 0.281
2.556ValPhe: 2.556 ± 0.189
4.905ValGly: 4.905 ± 0.334
1.54ValHis: 1.54 ± 0.182
3.445ValIle: 3.445 ± 0.207
3.524ValLys: 3.524 ± 0.218
5.509ValLeu: 5.509 ± 0.283
1.54ValMet: 1.54 ± 0.18
2.207ValAsn: 2.207 ± 0.21
3.524ValPro: 3.524 ± 0.279
2.254ValGln: 2.254 ± 0.176
4.588ValArg: 4.588 ± 0.279
4.112ValSer: 4.112 ± 0.25
4.604ValThr: 4.604 ± 0.351
5.017ValVal: 5.017 ± 0.359
1.127ValTrp: 1.127 ± 0.126
2.143ValTyr: 2.143 ± 0.191
0.0ValXaa: 0.0 ± 0.0
Trp
1.683TrpAla: 1.683 ± 0.159
0.079TrpCys: 0.079 ± 0.026
1.159TrpAsp: 1.159 ± 0.151
0.762TrpGlu: 0.762 ± 0.114
0.73TrpPhe: 0.73 ± 0.111
1.27TrpGly: 1.27 ± 0.13
0.254TrpHis: 0.254 ± 0.061
0.603TrpIle: 0.603 ± 0.09
0.953TrpLys: 0.953 ± 0.118
1.445TrpLeu: 1.445 ± 0.154
0.318TrpMet: 0.318 ± 0.065
0.746TrpAsn: 0.746 ± 0.128
0.794TrpPro: 0.794 ± 0.119
0.445TrpGln: 0.445 ± 0.094
1.159TrpArg: 1.159 ± 0.142
1.048TrpSer: 1.048 ± 0.131
1.222TrpThr: 1.222 ± 0.155
1.27TrpVal: 1.27 ± 0.158
0.27TrpTrp: 0.27 ± 0.068
0.254TrpTyr: 0.254 ± 0.053
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.556TyrAla: 3.556 ± 0.233
0.429TyrCys: 0.429 ± 0.086
2.254TyrAsp: 2.254 ± 0.241
1.969TyrGlu: 1.969 ± 0.189
0.953TyrPhe: 0.953 ± 0.151
2.302TyrGly: 2.302 ± 0.176
0.794TyrHis: 0.794 ± 0.157
1.334TyrIle: 1.334 ± 0.149
1.175TyrLys: 1.175 ± 0.15
2.318TyrLeu: 2.318 ± 0.183
0.556TyrMet: 0.556 ± 0.095
0.841TyrAsn: 0.841 ± 0.121
1.413TyrPro: 1.413 ± 0.146
0.937TyrGln: 0.937 ± 0.119
2.223TyrArg: 2.223 ± 0.183
1.445TyrSer: 1.445 ± 0.128
2.127TyrThr: 2.127 ± 0.207
2.35TyrVal: 2.35 ± 0.209
0.445TyrTrp: 0.445 ± 0.086
0.921TyrTyr: 0.921 ± 0.117
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 319 proteins (62993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski