Microbacterium phage Chepli

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; unclassified Neferthenavirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5NZ20|A0A4Y5NZ20_9CAUD aGPT-Pplase1 domain-containing protein OS=Microbacterium phage Chepli OX=2584495 GN=53 PE=4 SV=1
MM1 pKa = 7.75AAIDD5 pKa = 4.6DD6 pKa = 4.47LNGLLFPLPGYY17 pKa = 9.57PILTDD22 pKa = 3.25AMKK25 pKa = 10.5QRR27 pKa = 11.84ALATALIPDD36 pKa = 4.71GAGVWPGQPGYY47 pKa = 11.1QNTYY51 pKa = 9.32DD52 pKa = 4.01VYY54 pKa = 8.25WAAMSLVGYY63 pKa = 8.45LQSQPFVKK71 pKa = 10.33SSSSEE76 pKa = 3.8GTAVAVEE83 pKa = 4.02KK84 pKa = 10.31PDD86 pKa = 3.0WGGILSFFRR95 pKa = 11.84SQSVIAAASQSGPILNPIPIPDD117 pKa = 3.7SSHH120 pKa = 6.89VIRR123 pKa = 11.84TDD125 pKa = 3.03MSGGDD130 pKa = 3.39SYY132 pKa = 12.2GDD134 pKa = 3.23VDD136 pKa = 3.86TDD138 pKa = 4.18LGG140 pKa = 3.83

Molecular weight: 14.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5NYZ0|A0A4Y5NYZ0_9CAUD Exonuclease OS=Microbacterium phage Chepli OX=2584495 GN=38 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.94PIPVISWWEE12 pKa = 3.53RR13 pKa = 11.84AKK15 pKa = 10.8RR16 pKa = 11.84FTVWHH21 pKa = 7.2PINTDD26 pKa = 3.13PDD28 pKa = 3.49DD29 pKa = 4.25EE30 pKa = 4.52YY31 pKa = 11.68RR32 pKa = 11.84DD33 pKa = 4.12GPRR36 pKa = 11.84FWYY39 pKa = 8.0PTFDD43 pKa = 4.05LLMVALGVCAYY54 pKa = 10.16FIGSPLLNTLFPVWFVDD71 pKa = 3.48SLGVSIVISALLAAAGAIVPKK92 pKa = 10.62LFMLEE97 pKa = 3.99IVGKK101 pKa = 9.58LALVFLLGGYY111 pKa = 10.13AGTVASLSQNSGEE124 pKa = 3.92NWFVVITLFSLVWLLGPRR142 pKa = 11.84LSWLFRR148 pKa = 11.84RR149 pKa = 11.84LGKK152 pKa = 9.7WIALTAWGQRR162 pKa = 11.84LAVRR166 pKa = 11.84RR167 pKa = 11.84AFRR170 pKa = 11.84QATRR174 pKa = 11.84EE175 pKa = 3.7EE176 pKa = 4.2RR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84EE180 pKa = 3.78RR181 pKa = 3.83

Molecular weight: 20.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13028

42

809

203.6

22.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.909 ± 0.52

0.775 ± 0.117

6.202 ± 0.439

6.294 ± 0.437

2.779 ± 0.18

7.906 ± 0.339

1.95 ± 0.196

5.12 ± 0.439

4.582 ± 0.372

8.712 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.168

3.362 ± 0.248

5.02 ± 0.209

4.375 ± 0.229

6.095 ± 0.397

5.68 ± 0.333

6.901 ± 0.369

7.292 ± 0.346

2.034 ± 0.18

2.541 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski