Amino acid dipepetide frequency for Microbacterium phage Chepli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.745AlaAla: 11.745 ± 1.641
0.614AlaCys: 0.614 ± 0.203
4.836AlaAsp: 4.836 ± 0.83
6.218AlaGlu: 6.218 ± 0.592
2.994AlaPhe: 2.994 ± 0.399
7.983AlaGly: 7.983 ± 1.32
1.612AlaHis: 1.612 ± 0.403
5.527AlaIle: 5.527 ± 0.882
5.527AlaLys: 5.527 ± 0.982
10.824AlaLeu: 10.824 ± 1.347
2.533AlaMet: 2.533 ± 0.511
2.84AlaAsn: 2.84 ± 0.444
4.299AlaPro: 4.299 ± 0.579
3.454AlaGln: 3.454 ± 0.609
6.295AlaArg: 6.295 ± 0.76
5.066AlaSer: 5.066 ± 0.656
7.216AlaThr: 7.216 ± 0.935
5.757AlaVal: 5.757 ± 0.6
1.382AlaTrp: 1.382 ± 0.358
2.38AlaTyr: 2.38 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.921CysAla: 0.921 ± 0.23
0.077CysCys: 0.077 ± 0.069
0.614CysAsp: 0.614 ± 0.245
0.307CysGlu: 0.307 ± 0.151
0.0CysPhe: 0.0 ± 0.0
0.614CysGly: 0.614 ± 0.191
0.307CysHis: 0.307 ± 0.172
0.537CysIle: 0.537 ± 0.209
0.537CysLys: 0.537 ± 0.189
0.614CysLeu: 0.614 ± 0.236
0.077CysMet: 0.077 ± 0.067
0.307CysAsn: 0.307 ± 0.134
0.537CysPro: 0.537 ± 0.237
0.307CysGln: 0.307 ± 0.148
0.537CysArg: 0.537 ± 0.184
0.461CysSer: 0.461 ± 0.16
0.307CysThr: 0.307 ± 0.147
0.23CysVal: 0.23 ± 0.109
0.23CysTrp: 0.23 ± 0.126
0.23CysTyr: 0.23 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
4.376AspAla: 4.376 ± 0.659
0.384AspCys: 0.384 ± 0.139
5.297AspAsp: 5.297 ± 1.114
5.527AspGlu: 5.527 ± 1.291
1.766AspPhe: 1.766 ± 0.287
4.99AspGly: 4.99 ± 0.686
1.151AspHis: 1.151 ± 0.336
3.685AspIle: 3.685 ± 0.601
2.226AspLys: 2.226 ± 0.444
5.757AspLeu: 5.757 ± 0.851
1.535AspMet: 1.535 ± 0.305
1.535AspAsn: 1.535 ± 0.347
3.992AspPro: 3.992 ± 0.543
2.303AspGln: 2.303 ± 0.444
3.147AspArg: 3.147 ± 0.443
3.915AspSer: 3.915 ± 0.541
2.763AspThr: 2.763 ± 0.38
4.529AspVal: 4.529 ± 0.583
1.689AspTrp: 1.689 ± 0.385
1.459AspTyr: 1.459 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
6.832GluAla: 6.832 ± 1.029
0.614GluCys: 0.614 ± 0.232
5.757GluAsp: 5.757 ± 1.286
6.525GluGlu: 6.525 ± 1.469
1.766GluPhe: 1.766 ± 0.351
4.836GluGly: 4.836 ± 0.569
1.919GluHis: 1.919 ± 0.472
2.073GluIle: 2.073 ± 0.49
2.149GluLys: 2.149 ± 0.375
5.143GluLeu: 5.143 ± 0.625
1.075GluMet: 1.075 ± 0.275
2.149GluAsn: 2.149 ± 0.416
1.919GluPro: 1.919 ± 0.437
2.533GluGln: 2.533 ± 0.487
3.992GluArg: 3.992 ± 0.728
2.226GluSer: 2.226 ± 0.407
4.299GluThr: 4.299 ± 0.511
3.915GluVal: 3.915 ± 0.568
1.228GluTrp: 1.228 ± 0.302
1.996GluTyr: 1.996 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
1.842PheAla: 1.842 ± 0.358
0.23PheCys: 0.23 ± 0.127
1.842PheAsp: 1.842 ± 0.393
1.689PheGlu: 1.689 ± 0.32
0.384PhePhe: 0.384 ± 0.153
2.763PheGly: 2.763 ± 0.551
0.537PheHis: 0.537 ± 0.211
1.305PheIle: 1.305 ± 0.327
1.535PheLys: 1.535 ± 0.43
2.226PheLeu: 2.226 ± 0.461
0.844PheMet: 0.844 ± 0.236
0.844PheAsn: 0.844 ± 0.216
1.075PhePro: 1.075 ± 0.28
0.998PheGln: 0.998 ± 0.26
2.226PheArg: 2.226 ± 0.46
2.303PheSer: 2.303 ± 0.362
2.687PheThr: 2.687 ± 0.435
1.612PheVal: 1.612 ± 0.307
0.461PheTrp: 0.461 ± 0.182
0.384PheTyr: 0.384 ± 0.147
0.0PheXaa: 0.0 ± 0.0
Gly
6.985GlyAla: 6.985 ± 0.974
0.614GlyCys: 0.614 ± 0.207
4.606GlyAsp: 4.606 ± 0.603
3.685GlyGlu: 3.685 ± 0.477
2.687GlyPhe: 2.687 ± 0.431
5.911GlyGly: 5.911 ± 0.977
1.305GlyHis: 1.305 ± 0.325
3.761GlyIle: 3.761 ± 0.835
5.604GlyLys: 5.604 ± 0.707
6.755GlyLeu: 6.755 ± 0.974
2.303GlyMet: 2.303 ± 0.421
2.687GlyAsn: 2.687 ± 0.393
3.454GlyPro: 3.454 ± 0.478
3.224GlyGln: 3.224 ± 0.52
4.529GlyArg: 4.529 ± 0.683
4.606GlySer: 4.606 ± 0.584
6.295GlyThr: 6.295 ± 0.8
5.911GlyVal: 5.911 ± 0.771
1.689GlyTrp: 1.689 ± 0.315
2.456GlyTyr: 2.456 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
1.612HisAla: 1.612 ± 0.365
0.077HisCys: 0.077 ± 0.079
1.075HisAsp: 1.075 ± 0.227
1.075HisGlu: 1.075 ± 0.293
0.768HisPhe: 0.768 ± 0.237
1.919HisGly: 1.919 ± 0.401
0.384HisHis: 0.384 ± 0.197
0.998HisIle: 0.998 ± 0.259
1.075HisLys: 1.075 ± 0.264
1.612HisLeu: 1.612 ± 0.296
0.614HisMet: 0.614 ± 0.207
0.614HisAsn: 0.614 ± 0.28
1.075HisPro: 1.075 ± 0.354
1.075HisGln: 1.075 ± 0.345
0.998HisArg: 0.998 ± 0.294
0.537HisSer: 0.537 ± 0.161
1.228HisThr: 1.228 ± 0.354
1.075HisVal: 1.075 ± 0.251
0.461HisTrp: 0.461 ± 0.167
1.228HisTyr: 1.228 ± 0.322
0.0HisXaa: 0.0 ± 0.0
Ile
5.297IleAla: 5.297 ± 0.643
0.384IleCys: 0.384 ± 0.168
3.761IleAsp: 3.761 ± 0.53
2.687IleGlu: 2.687 ± 0.416
0.844IlePhe: 0.844 ± 0.355
4.145IleGly: 4.145 ± 0.629
0.691IleHis: 0.691 ± 0.234
3.838IleIle: 3.838 ± 0.86
2.456IleLys: 2.456 ± 0.45
3.071IleLeu: 3.071 ± 0.545
0.921IleMet: 0.921 ± 0.313
2.533IleAsn: 2.533 ± 0.495
3.301IlePro: 3.301 ± 0.602
1.612IleGln: 1.612 ± 0.423
3.301IleArg: 3.301 ± 0.525
3.071IleSer: 3.071 ± 0.51
4.376IleThr: 4.376 ± 0.746
2.994IleVal: 2.994 ± 0.743
0.844IleTrp: 0.844 ± 0.239
1.075IleTyr: 1.075 ± 0.28
0.0IleXaa: 0.0 ± 0.0
Lys
5.066LysAla: 5.066 ± 0.697
0.461LysCys: 0.461 ± 0.185
2.38LysAsp: 2.38 ± 0.509
2.84LysGlu: 2.84 ± 0.514
1.151LysPhe: 1.151 ± 0.333
3.378LysGly: 3.378 ± 0.572
0.998LysHis: 0.998 ± 0.253
1.612LysIle: 1.612 ± 0.303
2.303LysLys: 2.303 ± 0.499
4.452LysLeu: 4.452 ± 0.478
1.535LysMet: 1.535 ± 0.336
1.305LysAsn: 1.305 ± 0.319
2.763LysPro: 2.763 ± 0.555
2.073LysGln: 2.073 ± 0.402
3.071LysArg: 3.071 ± 0.521
2.687LysSer: 2.687 ± 0.519
3.147LysThr: 3.147 ± 0.492
3.071LysVal: 3.071 ± 0.334
0.998LysTrp: 0.998 ± 0.278
0.537LysTyr: 0.537 ± 0.179
0.0LysXaa: 0.0 ± 0.0
Leu
10.67LeuAla: 10.67 ± 1.024
0.691LeuCys: 0.691 ± 0.279
4.529LeuAsp: 4.529 ± 0.722
4.836LeuGlu: 4.836 ± 0.581
1.996LeuPhe: 1.996 ± 0.426
6.602LeuGly: 6.602 ± 0.844
1.612LeuHis: 1.612 ± 0.438
4.606LeuIle: 4.606 ± 0.628
3.531LeuLys: 3.531 ± 0.536
7.523LeuLeu: 7.523 ± 0.761
2.149LeuMet: 2.149 ± 0.322
3.071LeuAsn: 3.071 ± 0.453
5.066LeuPro: 5.066 ± 0.604
3.378LeuGln: 3.378 ± 0.4
5.681LeuArg: 5.681 ± 0.782
3.685LeuSer: 3.685 ± 0.459
6.371LeuThr: 6.371 ± 0.647
7.753LeuVal: 7.753 ± 0.845
1.689LeuTrp: 1.689 ± 0.254
1.689LeuTyr: 1.689 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
2.533MetAla: 2.533 ± 0.368
0.0MetCys: 0.0 ± 0.0
1.535MetAsp: 1.535 ± 0.37
1.535MetGlu: 1.535 ± 0.307
0.537MetPhe: 0.537 ± 0.183
2.073MetGly: 2.073 ± 0.412
0.384MetHis: 0.384 ± 0.156
0.844MetIle: 0.844 ± 0.258
0.921MetLys: 0.921 ± 0.288
2.687MetLeu: 2.687 ± 0.553
0.537MetMet: 0.537 ± 0.213
0.921MetAsn: 0.921 ± 0.227
1.535MetPro: 1.535 ± 0.235
1.305MetGln: 1.305 ± 0.311
1.228MetArg: 1.228 ± 0.351
1.996MetSer: 1.996 ± 0.353
1.535MetThr: 1.535 ± 0.395
1.766MetVal: 1.766 ± 0.297
0.384MetTrp: 0.384 ± 0.193
0.461MetTyr: 0.461 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.071AsnAla: 3.071 ± 0.466
0.077AsnCys: 0.077 ± 0.087
1.766AsnAsp: 1.766 ± 0.329
1.996AsnGlu: 1.996 ± 0.336
0.768AsnPhe: 0.768 ± 0.262
3.608AsnGly: 3.608 ± 0.648
0.921AsnHis: 0.921 ± 0.219
1.228AsnIle: 1.228 ± 0.442
1.151AsnLys: 1.151 ± 0.29
2.61AsnLeu: 2.61 ± 0.416
0.921AsnMet: 0.921 ± 0.254
1.228AsnAsn: 1.228 ± 0.302
2.38AsnPro: 2.38 ± 0.466
1.382AsnGln: 1.382 ± 0.294
1.766AsnArg: 1.766 ± 0.378
2.149AsnSer: 2.149 ± 0.349
2.38AsnThr: 2.38 ± 0.446
2.533AsnVal: 2.533 ± 0.436
0.537AsnTrp: 0.537 ± 0.215
1.151AsnTyr: 1.151 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
5.911ProAla: 5.911 ± 0.635
0.537ProCys: 0.537 ± 0.24
2.61ProAsp: 2.61 ± 0.451
3.301ProGlu: 3.301 ± 0.607
1.151ProPhe: 1.151 ± 0.306
4.759ProGly: 4.759 ± 0.682
0.461ProHis: 0.461 ± 0.169
2.917ProIle: 2.917 ± 0.46
1.919ProLys: 1.919 ± 0.469
4.145ProLeu: 4.145 ± 0.669
0.998ProMet: 0.998 ± 0.268
1.535ProAsn: 1.535 ± 0.332
1.689ProPro: 1.689 ± 0.377
2.456ProGln: 2.456 ± 0.643
2.763ProArg: 2.763 ± 0.534
2.84ProSer: 2.84 ± 0.303
4.376ProThr: 4.376 ± 0.626
3.685ProVal: 3.685 ± 0.434
1.151ProTrp: 1.151 ± 0.27
0.998ProTyr: 0.998 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
5.911GlnAla: 5.911 ± 0.635
0.154GlnCys: 0.154 ± 0.115
2.456GlnAsp: 2.456 ± 0.462
2.917GlnGlu: 2.917 ± 0.428
0.844GlnPhe: 0.844 ± 0.222
3.224GlnGly: 3.224 ± 0.468
1.151GlnHis: 1.151 ± 0.308
2.226GlnIle: 2.226 ± 0.548
1.305GlnLys: 1.305 ± 0.264
3.761GlnLeu: 3.761 ± 0.564
0.998GlnMet: 0.998 ± 0.285
1.842GlnAsn: 1.842 ± 0.329
1.612GlnPro: 1.612 ± 0.395
1.919GlnGln: 1.919 ± 0.489
3.147GlnArg: 3.147 ± 0.55
2.61GlnSer: 2.61 ± 0.507
1.689GlnThr: 1.689 ± 0.281
3.378GlnVal: 3.378 ± 0.559
0.921GlnTrp: 0.921 ± 0.308
1.689GlnTyr: 1.689 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
5.911ArgAla: 5.911 ± 0.685
0.768ArgCys: 0.768 ± 0.283
4.299ArgAsp: 4.299 ± 0.654
4.068ArgGlu: 4.068 ± 0.599
1.766ArgPhe: 1.766 ± 0.366
3.454ArgGly: 3.454 ± 0.521
1.151ArgHis: 1.151 ± 0.323
3.071ArgIle: 3.071 ± 0.441
3.531ArgLys: 3.531 ± 0.762
5.527ArgLeu: 5.527 ± 0.798
1.996ArgMet: 1.996 ± 0.387
1.842ArgAsn: 1.842 ± 0.351
2.61ArgPro: 2.61 ± 0.433
2.687ArgGln: 2.687 ± 0.399
4.376ArgArg: 4.376 ± 0.678
3.147ArgSer: 3.147 ± 0.407
3.454ArgThr: 3.454 ± 0.503
5.297ArgVal: 5.297 ± 0.661
0.844ArgTrp: 0.844 ± 0.333
1.151ArgTyr: 1.151 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
4.759SerAla: 4.759 ± 0.873
0.154SerCys: 0.154 ± 0.112
3.608SerAsp: 3.608 ± 0.516
2.687SerGlu: 2.687 ± 0.439
2.303SerPhe: 2.303 ± 0.444
5.22SerGly: 5.22 ± 0.626
0.768SerHis: 0.768 ± 0.224
3.071SerIle: 3.071 ± 0.524
2.38SerLys: 2.38 ± 0.448
5.681SerLeu: 5.681 ± 0.848
1.689SerMet: 1.689 ± 0.405
1.689SerAsn: 1.689 ± 0.407
2.38SerPro: 2.38 ± 0.449
3.147SerGln: 3.147 ± 0.501
2.917SerArg: 2.917 ± 0.379
3.224SerSer: 3.224 ± 0.619
3.531SerThr: 3.531 ± 0.713
4.759SerVal: 4.759 ± 0.636
0.844SerTrp: 0.844 ± 0.316
1.919SerTyr: 1.919 ± 0.449
0.0SerXaa: 0.0 ± 0.0
Thr
5.757ThrAla: 5.757 ± 0.809
0.537ThrCys: 0.537 ± 0.198
3.531ThrAsp: 3.531 ± 0.654
3.224ThrGlu: 3.224 ± 0.456
2.456ThrPhe: 2.456 ± 0.372
6.371ThrGly: 6.371 ± 0.661
1.535ThrHis: 1.535 ± 0.417
3.224ThrIle: 3.224 ± 0.389
2.226ThrLys: 2.226 ± 0.37
5.527ThrLeu: 5.527 ± 0.581
1.382ThrMet: 1.382 ± 0.295
1.842ThrAsn: 1.842 ± 0.437
4.452ThrPro: 4.452 ± 0.66
2.994ThrGln: 2.994 ± 0.437
4.529ThrArg: 4.529 ± 0.623
4.913ThrSer: 4.913 ± 0.744
6.141ThrThr: 6.141 ± 0.845
5.681ThrVal: 5.681 ± 0.665
1.151ThrTrp: 1.151 ± 0.339
1.535ThrTyr: 1.535 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.985ValAla: 6.985 ± 0.684
0.691ValCys: 0.691 ± 0.255
4.529ValAsp: 4.529 ± 0.566
4.836ValGlu: 4.836 ± 0.573
2.303ValPhe: 2.303 ± 0.53
4.376ValGly: 4.376 ± 0.608
1.382ValHis: 1.382 ± 0.371
4.222ValIle: 4.222 ± 0.784
2.994ValLys: 2.994 ± 0.498
5.757ValLeu: 5.757 ± 0.629
1.689ValMet: 1.689 ± 0.375
2.687ValAsn: 2.687 ± 0.525
3.761ValPro: 3.761 ± 0.581
4.529ValGln: 4.529 ± 0.698
3.685ValArg: 3.685 ± 0.512
4.606ValSer: 4.606 ± 0.683
4.299ValThr: 4.299 ± 0.525
5.297ValVal: 5.297 ± 0.664
2.456ValTrp: 2.456 ± 0.434
1.842ValTyr: 1.842 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.535TrpAla: 1.535 ± 0.314
0.307TrpCys: 0.307 ± 0.195
1.382TrpAsp: 1.382 ± 0.32
1.382TrpGlu: 1.382 ± 0.379
0.537TrpPhe: 0.537 ± 0.202
0.998TrpGly: 0.998 ± 0.277
0.844TrpHis: 0.844 ± 0.254
1.075TrpIle: 1.075 ± 0.334
1.075TrpLys: 1.075 ± 0.247
1.919TrpLeu: 1.919 ± 0.309
0.23TrpMet: 0.23 ± 0.162
0.921TrpAsn: 0.921 ± 0.232
0.691TrpPro: 0.691 ± 0.236
1.305TrpGln: 1.305 ± 0.28
0.844TrpArg: 0.844 ± 0.299
0.921TrpSer: 0.921 ± 0.335
1.459TrpThr: 1.459 ± 0.321
1.766TrpVal: 1.766 ± 0.417
0.461TrpTrp: 0.461 ± 0.218
0.691TrpTyr: 0.691 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.382TyrAla: 1.382 ± 0.319
0.384TyrCys: 0.384 ± 0.165
1.612TyrAsp: 1.612 ± 0.415
1.612TyrGlu: 1.612 ± 0.308
0.768TyrPhe: 0.768 ± 0.188
1.842TyrGly: 1.842 ± 0.314
0.384TyrHis: 0.384 ± 0.163
1.382TyrIle: 1.382 ± 0.293
1.382TyrLys: 1.382 ± 0.447
1.535TyrLeu: 1.535 ± 0.286
0.614TyrMet: 0.614 ± 0.188
1.228TyrAsn: 1.228 ± 0.239
1.612TyrPro: 1.612 ± 0.353
1.075TyrGln: 1.075 ± 0.293
1.919TyrArg: 1.919 ± 0.376
1.842TyrSer: 1.842 ± 0.425
1.459TyrThr: 1.459 ± 0.355
1.919TyrVal: 1.919 ± 0.365
0.921TyrTrp: 0.921 ± 0.232
0.537TyrTyr: 0.537 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski