Novosphingobium sp. AAP83

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1BX21|A0A0N1BX21_9SPHN Cytochrome OS=Novosphingobium sp. AAP83 OX=1523425 GN=IP81_17885 PE=3 SV=1
MM1 pKa = 7.55EE2 pKa = 5.31GLRR5 pKa = 11.84EE6 pKa = 4.23AKK8 pKa = 10.14LLCQTVSRR16 pKa = 11.84FAVEE20 pKa = 4.37RR21 pKa = 11.84FQRR24 pKa = 11.84LATEE28 pKa = 4.75VIAHH32 pKa = 6.36IQTYY36 pKa = 7.26PACALFGDD44 pKa = 5.08DD45 pKa = 4.89EE46 pKa = 4.46NASNLWDD53 pKa = 4.71DD54 pKa = 3.68YY55 pKa = 11.66CYY57 pKa = 10.8DD58 pKa = 3.63QQTGPVTFLEE68 pKa = 4.8FAWSDD73 pKa = 3.39VFEE76 pKa = 4.28PHH78 pKa = 6.62LKK80 pKa = 8.5EE81 pKa = 4.27TIGALDD87 pKa = 3.47RR88 pKa = 11.84SEE90 pKa = 4.25ATLLTFYY97 pKa = 10.73CCWDD101 pKa = 3.78LYY103 pKa = 10.85EE104 pKa = 5.2DD105 pKa = 5.38PINIVGEE112 pKa = 4.23PPNGIYY118 pKa = 10.43EE119 pKa = 4.39DD120 pKa = 3.86ALAAEE125 pKa = 4.75IMDD128 pKa = 3.72AVTRR132 pKa = 11.84VALNRR137 pKa = 11.84RR138 pKa = 11.84LPDD141 pKa = 3.87DD142 pKa = 4.55EE143 pKa = 5.42EE144 pKa = 6.97GEE146 pKa = 4.54DD147 pKa = 4.79DD148 pKa = 4.18WSEE151 pKa = 4.78DD152 pKa = 4.85DD153 pKa = 6.03DD154 pKa = 5.55PSDD157 pKa = 4.46DD158 pKa = 3.6

Molecular weight: 18.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1C2W5|A0A0N1C2W5_9SPHN Uncharacterized protein OS=Novosphingobium sp. AAP83 OX=1523425 GN=IP81_14740 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.32GFRR19 pKa = 11.84TRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 8.75ILAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97KK41 pKa = 10.66LSAA44 pKa = 4.03

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3633

0

3633

1192463

31

5349

328.2

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.876 ± 0.064

0.852 ± 0.013

5.899 ± 0.055

5.374 ± 0.04

3.717 ± 0.032

9.023 ± 0.101

1.96 ± 0.025

5.194 ± 0.028

3.245 ± 0.039

9.699 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.026

2.93 ± 0.037

5.068 ± 0.038

3.231 ± 0.024

6.612 ± 0.053

5.425 ± 0.043

5.433 ± 0.044

7.259 ± 0.029

1.43 ± 0.021

2.199 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski