Amino acid dipepetide frequency for Novosphingobium sp. AAP83

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.2AlaAla: 17.2 ± 0.189	1.026AlaCys: 1.026 ± 0.033	7.485AlaAsp: 7.485 ± 0.118	7.29AlaGlu: 7.29 ± 0.104	4.533AlaPhe: 4.533 ± 0.067	11.226AlaGly: 11.226 ± 0.179	2.285AlaHis: 2.285 ± 0.051	6.764AlaIle: 6.764 ± 0.081	4.483AlaLys: 4.483 ± 0.083	13.38AlaLeu: 13.38 ± 0.153	4.101AlaMet: 4.101 ± 0.065	3.747AlaAsn: 3.747 ± 0.072	5.739AlaPro: 5.739 ± 0.092	4.991AlaGln: 4.991 ± 0.069	8.617AlaArg: 8.617 ± 0.12	6.9AlaSer: 6.9 ± 0.095	6.435AlaThr: 6.435 ± 0.091	8.513AlaVal: 8.513 ± 0.099	1.619AlaTrp: 1.619 ± 0.041	2.425AlaTyr: 2.425 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.063CysAla: 1.063 ± 0.033	0.101CysCys: 0.101 ± 0.009	0.599CysAsp: 0.599 ± 0.025	0.461CysGlu: 0.461 ± 0.022	0.293CysPhe: 0.293 ± 0.015	0.907CysGly: 0.907 ± 0.031	0.254CysHis: 0.254 ± 0.02	0.409CysIle: 0.409 ± 0.021	0.221CysLys: 0.221 ± 0.016	0.761CysLeu: 0.761 ± 0.027	0.176CysMet: 0.176 ± 0.013	0.26CysAsn: 0.26 ± 0.015	0.471CysPro: 0.471 ± 0.022	0.225CysGln: 0.225 ± 0.016	0.509CysArg: 0.509 ± 0.02	0.468CysSer: 0.468 ± 0.021	0.437CysThr: 0.437 ± 0.021	0.594CysVal: 0.594 ± 0.023	0.127CysTrp: 0.127 ± 0.01	0.184CysTyr: 0.184 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.266AspAla: 7.266 ± 0.086	0.527AspCys: 0.527 ± 0.021	3.362AspAsp: 3.362 ± 0.096	3.362AspGlu: 3.362 ± 0.057	2.294AspPhe: 2.294 ± 0.053	5.637AspGly: 5.637 ± 0.108	1.311AspHis: 1.311 ± 0.037	2.969AspIle: 2.969 ± 0.057	1.874AspLys: 1.874 ± 0.045	5.624AspLeu: 5.624 ± 0.073	1.41AspMet: 1.41 ± 0.032	1.513AspAsn: 1.513 ± 0.046	3.494AspPro: 3.494 ± 0.06	1.8AspGln: 1.8 ± 0.04	4.073AspArg: 4.073 ± 0.055	2.487AspSer: 2.487 ± 0.052	3.023AspThr: 3.023 ± 0.171	4.321AspVal: 4.321 ± 0.085	1.083AspTrp: 1.083 ± 0.03	1.56AspTyr: 1.56 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	7.412GluAla: 7.412 ± 0.095	0.408GluCys: 0.408 ± 0.022	2.688GluAsp: 2.688 ± 0.052	2.789GluGlu: 2.789 ± 0.069	1.755GluPhe: 1.755 ± 0.038	4.513GluGly: 4.513 ± 0.07	1.14GluHis: 1.14 ± 0.037	2.954GluIle: 2.954 ± 0.062	2.063GluLys: 2.063 ± 0.045	5.1GluLeu: 5.1 ± 0.072	1.449GluMet: 1.449 ± 0.04	1.527GluAsn: 1.527 ± 0.038	2.377GluPro: 2.377 ± 0.052	2.144GluGln: 2.144 ± 0.042	4.388GluArg: 4.388 ± 0.085	2.23GluSer: 2.23 ± 0.039	3.056GluThr: 3.056 ± 0.05	3.824GluVal: 3.824 ± 0.061	0.844GluTrp: 0.844 ± 0.026	1.081GluTyr: 1.081 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	5.059PheAla: 5.059 ± 0.072	0.357PheCys: 0.357 ± 0.015	2.755PheAsp: 2.755 ± 0.055	2.091PheGlu: 2.091 ± 0.044	1.346PhePhe: 1.346 ± 0.04	3.969PheGly: 3.969 ± 0.073	0.725PheHis: 0.725 ± 0.026	1.606PheIle: 1.606 ± 0.033	1.021PheLys: 1.021 ± 0.031	3.077PheLeu: 3.077 ± 0.059	0.832PheMet: 0.832 ± 0.026	1.26PheAsn: 1.26 ± 0.036	1.541PhePro: 1.541 ± 0.043	0.97PheGln: 0.97 ± 0.028	2.085PheArg: 2.085 ± 0.039	2.19PheSer: 2.19 ± 0.051	2.176PheThr: 2.176 ± 0.051	2.61PheVal: 2.61 ± 0.048	0.533PheTrp: 0.533 ± 0.021	0.972PheTyr: 0.972 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.102GlyAla: 10.102 ± 0.167	0.845GlyCys: 0.845 ± 0.031	5.042GlyAsp: 5.042 ± 0.181	4.654GlyGlu: 4.654 ± 0.069	3.738GlyPhe: 3.738 ± 0.068	8.504GlyGly: 8.504 ± 0.27	1.814GlyHis: 1.814 ± 0.043	4.717GlyIle: 4.717 ± 0.069	3.666GlyLys: 3.666 ± 0.068	8.945GlyLeu: 8.945 ± 0.101	2.527GlyMet: 2.527 ± 0.051	2.748GlyAsn: 2.748 ± 0.138	3.577GlyPro: 3.577 ± 0.063	3.125GlyGln: 3.125 ± 0.052	5.619GlyArg: 5.619 ± 0.074	5.123GlySer: 5.123 ± 0.138	5.137GlyThr: 5.137 ± 0.108	6.407GlyVal: 6.407 ± 0.088	1.576GlyTrp: 1.576 ± 0.037	2.362GlyTyr: 2.362 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.354HisAla: 2.354 ± 0.056	0.236HisCys: 0.236 ± 0.016	1.187HisAsp: 1.187 ± 0.033	0.991HisGlu: 0.991 ± 0.031	0.833HisPhe: 0.833 ± 0.027	1.909HisGly: 1.909 ± 0.05	0.512HisHis: 0.512 ± 0.026	0.935HisIle: 0.935 ± 0.029	0.545HisLys: 0.545 ± 0.023	1.798HisLeu: 1.798 ± 0.045	0.489HisMet: 0.489 ± 0.021	0.517HisAsn: 0.517 ± 0.022	1.212HisPro: 1.212 ± 0.037	0.524HisGln: 0.524 ± 0.023	1.361HisArg: 1.361 ± 0.04	0.98HisSer: 0.98 ± 0.031	0.819HisThr: 0.819 ± 0.029	1.479HisVal: 1.479 ± 0.04	0.346HisTrp: 0.346 ± 0.018	0.57HisTyr: 0.57 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.943IleAla: 7.943 ± 0.106	0.507IleCys: 0.507 ± 0.023	4.0IleAsp: 4.0 ± 0.058	3.596IleGlu: 3.596 ± 0.059	1.501IlePhe: 1.501 ± 0.04	5.194IleGly: 5.194 ± 0.08	0.907IleHis: 0.907 ± 0.03	2.237IleIle: 2.237 ± 0.045	1.429IleLys: 1.429 ± 0.036	3.78IleLeu: 3.78 ± 0.064	1.026IleMet: 1.026 ± 0.034	1.676IleAsn: 1.676 ± 0.046	2.265IlePro: 2.265 ± 0.048	1.137IleGln: 1.137 ± 0.03	3.037IleArg: 3.037 ± 0.052	2.939IleSer: 2.939 ± 0.06	2.975IleThr: 2.975 ± 0.076	4.097IleVal: 4.097 ± 0.053	0.614IleTrp: 0.614 ± 0.024	1.084IleTyr: 1.084 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.491LysAla: 4.491 ± 0.081	0.19LysCys: 0.19 ± 0.013	1.686LysAsp: 1.686 ± 0.049	1.351LysGlu: 1.351 ± 0.036	1.019LysPhe: 1.019 ± 0.033	2.808LysGly: 2.808 ± 0.053	0.626LysHis: 0.626 ± 0.026	1.628LysIle: 1.628 ± 0.046	1.085LysLys: 1.085 ± 0.035	3.419LysLeu: 3.419 ± 0.068	0.88LysMet: 0.88 ± 0.031	0.773LysAsn: 0.773 ± 0.028	2.113LysPro: 2.113 ± 0.049	0.98LysGln: 0.98 ± 0.032	2.315LysArg: 2.315 ± 0.047	1.713LysSer: 1.713 ± 0.039	1.794LysThr: 1.794 ± 0.041	2.575LysVal: 2.575 ± 0.06	0.407LysTrp: 0.407 ± 0.018	0.595LysTyr: 0.595 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.545LeuAla: 13.545 ± 0.149	0.851LeuCys: 0.851 ± 0.026	5.895LeuAsp: 5.895 ± 0.073	4.981LeuGlu: 4.981 ± 0.075	3.425LeuPhe: 3.425 ± 0.059	8.596LeuGly: 8.596 ± 0.098	1.798LeuHis: 1.798 ± 0.047	4.855LeuIle: 4.855 ± 0.074	3.105LeuLys: 3.105 ± 0.063	8.786LeuLeu: 8.786 ± 0.134	2.26LeuMet: 2.26 ± 0.041	2.722LeuAsn: 2.722 ± 0.055	5.504LeuPro: 5.504 ± 0.09	2.606LeuGln: 2.606 ± 0.047	6.361LeuArg: 6.361 ± 0.091	5.994LeuSer: 5.994 ± 0.079	5.5LeuThr: 5.5 ± 0.085	7.116LeuVal: 7.116 ± 0.084	1.196LeuTrp: 1.196 ± 0.032	1.894LeuTyr: 1.894 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.534MetAla: 3.534 ± 0.059	0.185MetCys: 0.185 ± 0.012	1.234MetAsp: 1.234 ± 0.03	1.166MetGlu: 1.166 ± 0.032	0.792MetPhe: 0.792 ± 0.027	2.054MetGly: 2.054 ± 0.051	0.468MetHis: 0.468 ± 0.02	1.556MetIle: 1.556 ± 0.039	0.958MetLys: 0.958 ± 0.032	2.658MetLeu: 2.658 ± 0.055	0.714MetMet: 0.714 ± 0.027	0.745MetAsn: 0.745 ± 0.024	1.488MetPro: 1.488 ± 0.039	0.865MetGln: 0.865 ± 0.031	1.801MetArg: 1.801 ± 0.04	1.463MetSer: 1.463 ± 0.035	1.743MetThr: 1.743 ± 0.035	1.737MetVal: 1.737 ± 0.04	0.267MetTrp: 0.267 ± 0.016	0.3MetTyr: 0.3 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.866AsnAla: 3.866 ± 0.078	0.265AsnCys: 0.265 ± 0.015	1.788AsnAsp: 1.788 ± 0.107	1.223AsnGlu: 1.223 ± 0.034	1.176AsnPhe: 1.176 ± 0.035	2.843AsnGly: 2.843 ± 0.072	0.516AsnHis: 0.516 ± 0.022	1.445AsnIle: 1.445 ± 0.036	0.749AsnLys: 0.749 ± 0.028	2.748AsnLeu: 2.748 ± 0.063	0.609AsnMet: 0.609 ± 0.022	0.915AsnAsn: 0.915 ± 0.043	2.048AsnPro: 2.048 ± 0.047	0.822AsnGln: 0.822 ± 0.032	1.959AsnArg: 1.959 ± 0.048	1.404AsnSer: 1.404 ± 0.046	1.472AsnThr: 1.472 ± 0.048	2.212AsnVal: 2.212 ± 0.055	0.481AsnTrp: 0.481 ± 0.021	0.761AsnTyr: 0.761 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	6.485ProAla: 6.485 ± 0.094	0.351ProCys: 0.351 ± 0.018	3.518ProAsp: 3.518 ± 0.068	3.562ProGlu: 3.562 ± 0.061	1.902ProPhe: 1.902 ± 0.038	4.339ProGly: 4.339 ± 0.065	1.021ProHis: 1.021 ± 0.034	2.314ProIle: 2.314 ± 0.043	1.634ProLys: 1.634 ± 0.042	4.819ProLeu: 4.819 ± 0.066	1.274ProMet: 1.274 ± 0.035	1.339ProAsn: 1.339 ± 0.038	2.427ProPro: 2.427 ± 0.064	1.826ProGln: 1.826 ± 0.045	2.74ProArg: 2.74 ± 0.058	2.66ProSer: 2.66 ± 0.054	2.439ProThr: 2.439 ± 0.045	4.291ProVal: 4.291 ± 0.066	0.696ProTrp: 0.696 ± 0.023	1.046ProTyr: 1.046 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.183GlnAla: 4.183 ± 0.075	0.248GlnCys: 0.248 ± 0.015	1.505GlnAsp: 1.505 ± 0.038	1.384GlnGlu: 1.384 ± 0.038	1.307GlnPhe: 1.307 ± 0.037	2.683GlnGly: 2.683 ± 0.048	0.629GlnHis: 0.629 ± 0.022	1.917GlnIle: 1.917 ± 0.038	1.009GlnLys: 1.009 ± 0.031	3.042GlnLeu: 3.042 ± 0.05	0.889GlnMet: 0.889 ± 0.028	0.909GlnAsn: 0.909 ± 0.03	1.738GlnPro: 1.738 ± 0.043	1.2GlnGln: 1.2 ± 0.037	2.346GlnArg: 2.346 ± 0.049	1.91GlnSer: 1.91 ± 0.047	1.848GlnThr: 1.848 ± 0.044	2.419GlnVal: 2.419 ± 0.051	0.515GlnTrp: 0.515 ± 0.02	0.631GlnTyr: 0.631 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	7.696ArgAla: 7.696 ± 0.119	0.533ArgCys: 0.533 ± 0.024	3.8ArgAsp: 3.8 ± 0.07	3.748ArgGlu: 3.748 ± 0.07	2.923ArgPhe: 2.923 ± 0.05	4.66ArgGly: 4.66 ± 0.069	1.487ArgHis: 1.487 ± 0.041	3.961ArgIle: 3.961 ± 0.065	2.379ArgLys: 2.379 ± 0.049	7.048ArgLeu: 7.048 ± 0.105	1.886ArgMet: 1.886 ± 0.041	2.041ArgAsn: 2.041 ± 0.044	3.106ArgPro: 3.106 ± 0.051	2.346ArgGln: 2.346 ± 0.046	4.564ArgArg: 4.564 ± 0.081	3.361ArgSer: 3.361 ± 0.056	3.327ArgThr: 3.327 ± 0.051	4.435ArgVal: 4.435 ± 0.068	1.079ArgTrp: 1.079 ± 0.037	1.741ArgTyr: 1.741 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.873SerAla: 6.873 ± 0.118	0.437SerCys: 0.437 ± 0.022	3.178SerAsp: 3.178 ± 0.056	2.593SerGlu: 2.593 ± 0.05	2.273SerPhe: 2.273 ± 0.043	5.898SerGly: 5.898 ± 0.139	0.989SerHis: 0.989 ± 0.03	2.811SerIle: 2.811 ± 0.053	1.495SerLys: 1.495 ± 0.037	5.308SerLeu: 5.308 ± 0.066	1.227SerMet: 1.227 ± 0.035	1.639SerAsn: 1.639 ± 0.045	2.785SerPro: 2.785 ± 0.056	1.623SerGln: 1.623 ± 0.043	3.321SerArg: 3.321 ± 0.055	2.981SerSer: 2.981 ± 0.074	2.73SerThr: 2.73 ± 0.063	4.017SerVal: 4.017 ± 0.068	0.743SerTrp: 0.743 ± 0.029	1.333SerTyr: 1.333 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	6.626ThrAla: 6.626 ± 0.101	0.445ThrCys: 0.445 ± 0.022	2.86ThrAsp: 2.86 ± 0.052	2.473ThrGlu: 2.473 ± 0.052	2.051ThrPhe: 2.051 ± 0.048	5.77ThrGly: 5.77 ± 0.141	0.974ThrHis: 0.974 ± 0.029	3.094ThrIle: 3.094 ± 0.071	1.444ThrLys: 1.444 ± 0.033	5.958ThrLeu: 5.958 ± 0.143	1.334ThrMet: 1.334 ± 0.037	1.545ThrAsn: 1.545 ± 0.048	3.152ThrPro: 3.152 ± 0.053	1.585ThrGln: 1.585 ± 0.043	3.189ThrArg: 3.189 ± 0.059	2.917ThrSer: 2.917 ± 0.064	2.833ThrThr: 2.833 ± 0.067	4.219ThrVal: 4.219 ± 0.073	0.638ThrTrp: 0.638 ± 0.022	1.226ThrTyr: 1.226 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	8.994ValAla: 8.994 ± 0.102	0.625ValCys: 0.625 ± 0.024	4.184ValAsp: 4.184 ± 0.056	4.288ValGlu: 4.288 ± 0.064	2.525ValPhe: 2.525 ± 0.051	5.552ValGly: 5.552 ± 0.088	1.28ValHis: 1.28 ± 0.038	4.178ValIle: 4.178 ± 0.064	2.168ValLys: 2.168 ± 0.05	7.004ValLeu: 7.004 ± 0.09	1.805ValMet: 1.805 ± 0.04	2.323ValAsn: 2.323 ± 0.061	3.91ValPro: 3.91 ± 0.054	2.177ValGln: 2.177 ± 0.039	4.917ValArg: 4.917 ± 0.065	4.34ValSer: 4.34 ± 0.079	4.656ValThr: 4.656 ± 0.087	5.344ValVal: 5.344 ± 0.082	0.965ValTrp: 0.965 ± 0.03	1.354ValTyr: 1.354 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.397TrpAla: 1.397 ± 0.039	0.149TrpCys: 0.149 ± 0.012	0.762TrpAsp: 0.762 ± 0.024	0.615TrpGlu: 0.615 ± 0.022	0.57TrpPhe: 0.57 ± 0.024	0.982TrpGly: 0.982 ± 0.036	0.332TrpHis: 0.332 ± 0.019	0.699TrpIle: 0.699 ± 0.027	0.498TrpLys: 0.498 ± 0.023	1.676TrpLeu: 1.676 ± 0.04	0.382TrpMet: 0.382 ± 0.018	0.481TrpAsn: 0.481 ± 0.02	0.694TrpPro: 0.694 ± 0.026	0.67TrpGln: 0.67 ± 0.025	1.233TrpArg: 1.233 ± 0.033	0.921TrpSer: 0.921 ± 0.036	0.813TrpThr: 0.813 ± 0.023	0.867TrpVal: 0.867 ± 0.029	0.249TrpTrp: 0.249 ± 0.015	0.305TrpTyr: 0.305 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.67TyrAla: 2.67 ± 0.05	0.234TyrCys: 0.234 ± 0.014	1.463TyrAsp: 1.463 ± 0.031	1.124TyrGlu: 1.124 ± 0.031	0.917TyrPhe: 0.917 ± 0.03	2.183TyrGly: 2.183 ± 0.069	0.533TyrHis: 0.533 ± 0.022	0.896TyrIle: 0.896 ± 0.027	0.623TyrLys: 0.623 ± 0.026	2.06TyrLeu: 2.06 ± 0.044	0.46TyrMet: 0.46 ± 0.02	0.62TyrAsn: 0.62 ± 0.026	1.041TyrPro: 1.041 ± 0.027	0.695TyrGln: 0.695 ± 0.029	1.686TyrArg: 1.686 ± 0.04	1.274TyrSer: 1.274 ± 0.041	1.12TyrThr: 1.12 ± 0.036	1.508TyrVal: 1.508 ± 0.04	0.319TyrTrp: 0.319 ± 0.016	0.57TyrTyr: 0.57 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3633 proteins (1192463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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