Colletotrichum orchidophilum
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14495 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G4BEV4|A0A1G4BEV4_9PEZI Cation transporter OS=Colletotrichum orchidophilum OX=1209926 GN=CORC01_04852 PE=4 SV=1
MM1 pKa = 7.49 PGLWKK6 pKa = 10.67 LLLPLLLLGGRR17 pKa = 11.84 SLAQTCLDD25 pKa = 4.17 ALNNDD30 pKa = 3.25 QVDD33 pKa = 3.51 ACVCYY38 pKa = 10.17 SLEE41 pKa = 4.19 YY42 pKa = 9.39 VVSYY46 pKa = 9.97 VAYY49 pKa = 8.72 IAVSTSYY56 pKa = 10.76 TSNCEE61 pKa = 3.91 ALPTLSATTSAAARR75 pKa = 11.84 FLEE78 pKa = 4.44 SNLGGGNYY86 pKa = 9.73 SGYY89 pKa = 8.68 VTISNGVYY97 pKa = 9.65 TGTDD101 pKa = 2.77 ILAGPTTRR109 pKa = 11.84 AILGLDD115 pKa = 3.42 GSGTLVVEE123 pKa = 4.96 DD124 pKa = 4.45 PPSYY128 pKa = 8.34 TTVSRR133 pKa = 11.84 TGTATVSPVSLPSFINSTSFLTTNPITAPQTILVANPTPGASIASRR179 pKa = 11.84 GTVVVAVPDD188 pKa = 3.63 PRR190 pKa = 11.84 LYY192 pKa = 9.77 NTSTLAYY199 pKa = 10.16 AGTDD203 pKa = 3.36 PLGAARR209 pKa = 11.84 TTISTIAEE217 pKa = 4.12 SGFVPGTYY225 pKa = 9.8 VVAVATTTVPDD236 pKa = 3.15 VSYY239 pKa = 11.13 DD240 pKa = 3.54 VSTVAYY246 pKa = 8.22 TGTAALSGPTVVSVIQPTNRR266 pKa = 11.84 SGRR269 pKa = 11.84 GTVVIGVPPTGVPPPQVTSIVPYY292 pKa = 9.86 EE293 pKa = 4.26 GTSSITGATTILNRR307 pKa = 11.84 PFDD310 pKa = 3.78 GTVPGTVVVGLPPSSFITQTVPFTGKK336 pKa = 9.9 EE337 pKa = 4.01 VLTAPITISIIPPSGSQLGTVVVGSPSATFLASFVPFTGSTQEE380 pKa = 4.21 TLSVIQPTGTSPGIVIIGQPGDD402 pKa = 3.56 PYY404 pKa = 11.76 ADD406 pKa = 3.63 VNDD409 pKa = 4.04 PTLTSASQVMPTRR422 pKa = 11.84 YY423 pKa = 7.86 VTSIVTYY430 pKa = 9.33 TPASDD435 pKa = 4.29 APNTPSTLAVIDD447 pKa = 3.66 GTVIIGLPRR456 pKa = 11.84 IISPAATGSDD466 pKa = 3.45 SFITSARR473 pKa = 11.84 GSFVTSTIDD482 pKa = 3.4 FTEE485 pKa = 4.78 GADD488 pKa = 3.75 LPTGVDD494 pKa = 3.57 PVGTIPGTVFVYY506 pKa = 10.67 APSAARR512 pKa = 11.84 DD513 pKa = 3.68 LGPSFVTSEE522 pKa = 4.25 VILLGTAAGSAPTTVSIVEE541 pKa = 4.3 PTDD544 pKa = 3.65 GLPGTIIIGRR554 pKa = 11.84 PAIFVTSTVPWTGSIPLPSRR574 pKa = 11.84 TTVAVVAPSGDD585 pKa = 3.62 SPGTIFVADD594 pKa = 3.62 PTSFGRR600 pKa = 11.84 QFSTSFVRR608 pKa = 11.84 LSGSLEE614 pKa = 3.77 EE615 pKa = 4.78 GAATTLTLIPGQGTSQPVVVIGVPDD640 pKa = 4.29 DD641 pKa = 4.79 YY642 pKa = 10.9 ITSTVDD648 pKa = 3.53 FSGTGILTTRR658 pKa = 11.84 TTLSTLPPNGVAPGVIIVADD678 pKa = 4.23 PPTSVSPGSAVVTAGVSDD696 pKa = 4.22 GTQPNYY702 pKa = 9.17 VTSTVVYY709 pKa = 10.08 GGLTTSVLTSVPPSGSNRR727 pKa = 11.84 GTVVLAVPSAAAGNPSPTPVSQLNNPSVARR757 pKa = 11.84 ASFVTSTIVSLVGSLVASRR776 pKa = 11.84 ATISTVTPDD785 pKa = 3.26 IANSIPGTYY794 pKa = 9.75 VVALPNAYY802 pKa = 7.99 ITSTVPWTGEE812 pKa = 4.05 VPLSTVTTISIIPPSNFQPGVIVVAAPNAIDD843 pKa = 3.85 SSTTQAVGSGTVPTPVLASNASPGNSPTPLSVTQLPAQTLFTTSTVRR890 pKa = 11.84 LASDD894 pKa = 4.17 LGISSATILTTQQASPGSPSTVLLGVPDD922 pKa = 3.52 QVANSEE928 pKa = 4.08 ASTAGAEE935 pKa = 4.22 AEE937 pKa = 4.5 TIINPNSGPQPASSSAAVFTSTATEE962 pKa = 3.83 SSIAFSASVGSVPSTTVLGNTALSSFVISDD992 pKa = 3.39 IFYY995 pKa = 9.41 TGTNSFTSPIPLTTLSADD1013 pKa = 3.6 LGSPMTVILLQPSSAIGSTGNPVGGSGSTSSLATGSSPTTSASPFSNVAGTAFSTIASTSVLADD1077 pKa = 3.28 SSISTTASSEE1087 pKa = 4.07 RR1088 pKa = 11.84 FSNQSPFGTPPDD1100 pKa = 3.9 SAVTTPLATVFVDD1113 pKa = 3.49 YY1114 pKa = 10.43 TGTGVPSEE1122 pKa = 4.2 FLTLRR1127 pKa = 11.84 TTSGTVVIQRR1137 pKa = 11.84 PRR1139 pKa = 11.84 PLTTVQTFYY1148 pKa = 11.01 SGSVSIAILTIIPSGTEE1165 pKa = 2.99 AATIVFGVPSPVNTTPVTSFSQEE1188 pKa = 4.08 SLSVEE1193 pKa = 4.35 LPSSTNSIDD1202 pKa = 3.59 TLISADD1208 pKa = 3.49 PQIIVSSTARR1218 pKa = 11.84 SSTPVAVPGTSTGTNQTSSEE1238 pKa = 4.14 LVADD1242 pKa = 3.92 PTSQISSPISSTRR1255 pKa = 11.84 SSSSEE1260 pKa = 4.02 TISSSNNSGAGSPALSAILSRR1281 pKa = 11.84 VSSTLTSLDD1290 pKa = 3.31 STIEE1294 pKa = 4.04 SSSSVLAQPQATSLSSLTASSTANTATATTVTDD1327 pKa = 3.69 AALTSNSVSEE1337 pKa = 4.49 SSSSSFSPILSGSVAPTASSSQSTSSGAISLSIISLDD1374 pKa = 3.48 VSTSDD1379 pKa = 4.37 PSSQGSGTFTSNTPPPSLLSTSISITADD1407 pKa = 3.18 PNLISSILSTATSLSTSDD1425 pKa = 3.57 AAFSASSGGLSLSATVEE1442 pKa = 4.01 VSVEE1446 pKa = 4.17 STVSSTAARR1455 pKa = 11.84 TPSTPSTSPSTSLSSVLDD1473 pKa = 3.49 ILSDD1477 pKa = 3.51 TTASSFSSSSGSGSQSSTSSDD1498 pKa = 2.88 ATASTSPSSEE1508 pKa = 3.87 LSIQNGPSSNLASTDD1523 pKa = 3.14 VPAPSDD1529 pKa = 3.78 SVVSSTALPNILSSLSEE1546 pKa = 4.44 SIEE1549 pKa = 4.11 SSSTLISSSSGTSTSTGTDD1568 pKa = 2.85 STTAEE1573 pKa = 4.18 TNAVSNDD1580 pKa = 2.97 SSAQSMNPVSSNPSDD1595 pKa = 3.54 SSTSSLDD1602 pKa = 3.17 PGISVGSSGLIGSTTTSGLSLVTPTATVVTSVVVFTGTEE1641 pKa = 4.15 SFTGTGLVPLTTIYY1655 pKa = 10.26 PSNSEE1660 pKa = 3.82 APTVVFGAARR1670 pKa = 11.84 EE1671 pKa = 4.29 LFSSVAVPYY1680 pKa = 10.29 SGTGIITGPQQVSIVLPSGSDD1701 pKa = 3.36 SLVLGTIVYY1710 pKa = 7.71 ATPDD1714 pKa = 3.58 PFVTSEE1720 pKa = 3.5 VDD1722 pKa = 3.35 YY1723 pKa = 10.53 TGTGTLVSPLPLTTIYY1739 pKa = 10.75 QSGTVPGTVILARR1752 pKa = 11.84 PAIFATSSTSSSTHH1766 pKa = 4.52 STASSEE1772 pKa = 4.15 SSISIEE1778 pKa = 4.8 LSTTSQSRR1786 pKa = 11.84 SSSDD1790 pKa = 3.18 SNASLEE1796 pKa = 4.4 TSSSFASSSQLEE1808 pKa = 4.36 STPSSTSSEE1817 pKa = 4.27 SLLGPSNIPEE1827 pKa = 4.41 STTSSTPTVLAEE1839 pKa = 4.22 SGTASEE1845 pKa = 4.5 SQSPSSEE1852 pKa = 4.11 LASTAPSTTSEE1863 pKa = 4.48 GVSSTIGEE1871 pKa = 4.34 LSSSTIISASSIGSSSSLGPSSSIAALTDD1900 pKa = 3.53 ASLSTGVSPDD1910 pKa = 3.44 PAFLSTSPISISDD1923 pKa = 3.64 STSPTLTAEE1932 pKa = 4.22 SSISSSRR1939 pKa = 11.84 TTLSTVPDD1947 pKa = 3.73 TTTSQRR1953 pKa = 11.84 SPFSTEE1959 pKa = 3.44 ISSSSQAPEE1968 pKa = 3.65 ISSQQSSDD1976 pKa = 3.27 FTALSSSTASSTTPLPAFSSTTEE1999 pKa = 3.83 SSSIEE2004 pKa = 4.06 AATSPSTADD2013 pKa = 3.02 ASMTSSSSSTDD2024 pKa = 3.05 STGMLEE2030 pKa = 3.95 ATTGFEE2036 pKa = 4.26 ATSGATSSSGVEE2048 pKa = 4.1 SGSATDD2054 pKa = 3.34 TGTTGAAFASLLTTSGSSSSSDD2076 pKa = 2.69 IAATSNIADD2085 pKa = 3.92 PRR2087 pKa = 11.84 SSSTLSDD2094 pKa = 3.51 VLSSEE2099 pKa = 4.5 TSVSTSISIEE2109 pKa = 4.13 SEE2111 pKa = 3.86 ASSATSMDD2119 pKa = 3.27 TTTTEE2124 pKa = 4.36 LTSSTSTIASSLAIEE2139 pKa = 4.58 SLTTPIVTTSEE2150 pKa = 4.11 TTSNDD2155 pKa = 2.25 ISRR2158 pKa = 11.84 SISTTSITSEE2168 pKa = 4.03 SDD2170 pKa = 3.27 QSSSTSDD2177 pKa = 2.9 IEE2179 pKa = 4.62 TVSTSYY2185 pKa = 11.56 ASLIPTTDD2193 pKa = 3.06 ASSLSGISITAGGTTSEE2210 pKa = 4.54 PSSSVADD2217 pKa = 3.38 ITSLISSTDD2226 pKa = 3.31 IPPTTPTSLFSEE2238 pKa = 4.51 ATSTSSEE2245 pKa = 4.08 ATTSTGDD2252 pKa = 3.61 PPSTPSSSLADD2263 pKa = 3.56 AASSLSLTDD2272 pKa = 3.71 ADD2274 pKa = 4.41 TVTSSSLLPTSSGLATATPISSEE2297 pKa = 4.1 TSPVSITTASVDD2309 pKa = 3.07 AASYY2313 pKa = 8.1 VTSQLIYY2320 pKa = 9.48 TGPSSLSGPLPLSTIQPTNGSPGTVILAFPASFYY2354 pKa = 7.6 TTSTSIYY2361 pKa = 9.55 TEE2363 pKa = 4.09 GDD2365 pKa = 3.53 SYY2367 pKa = 11.26 IVPSAISTVAPSGIAKK2383 pKa = 8.36 GTVIFAARR2391 pKa = 11.84 YY2392 pKa = 7.76 AVARR2396 pKa = 11.84 VVYY2399 pKa = 10.05 SGTSVITEE2407 pKa = 5.29 AIPVATYY2414 pKa = 9.43 PGSGTIIGTIVYY2426 pKa = 9.15 AYY2428 pKa = 7.57 PPPSLATSEE2437 pKa = 4.48 EE2438 pKa = 4.57 STSGASTTASTEE2450 pKa = 4.06 PLSSITPQVNSTSSTSSDD2468 pKa = 3.43 VNFSSTPSSSSTLSTFNLWIDD2489 pKa = 3.96 DD2490 pKa = 3.93 RR2491 pKa = 11.84 GADD2494 pKa = 3.38 NVEE2497 pKa = 3.68 RR2498 pKa = 11.84 ATFRR2502 pKa = 11.84 DD2503 pKa = 3.93 YY2504 pKa = 10.91 IITIDD2509 pKa = 3.5 RR2510 pKa = 11.84 FYY2512 pKa = 11.66 GLLSFIDD2519 pKa = 4.12 FVYY2522 pKa = 10.94 SS2523 pKa = 3.39
Molecular weight: 250.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.012
Thurlkill 3.516
EMBOSS 3.605
Sillero 3.808
Patrickios 1.341
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A1G4B2K1|A0A1G4B2K1_9PEZI Uncharacterized protein OS=Colletotrichum orchidophilum OX=1209926 GN=CORC01_09176 PE=4 SV=1
MM1 pKa = 7.85 PLRR4 pKa = 11.84 STRR7 pKa = 11.84 HH8 pKa = 4.05 TTTTAPRR15 pKa = 11.84 RR16 pKa = 11.84 SIFSRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 APAHH27 pKa = 5.9 TSHH30 pKa = 6.67 HH31 pKa = 5.83 HH32 pKa = 5.27 TTTKK36 pKa = 7.05 TTRR39 pKa = 11.84 TTKK42 pKa = 10.47 RR43 pKa = 11.84 GGGGLFSRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 GPVHH57 pKa = 6.22 TAPVHH62 pKa = 4.58 HH63 pKa = 6.26 QRR65 pKa = 11.84 RR66 pKa = 11.84 KK67 pKa = 9.81 PSMGDD72 pKa = 3.3 KK73 pKa = 10.53 ISGAMLKK80 pKa = 10.72 LKK82 pKa = 9.36 GTLTRR87 pKa = 11.84 RR88 pKa = 11.84 PGQKK92 pKa = 9.9 AAGTRR97 pKa = 11.84 RR98 pKa = 11.84 MHH100 pKa = 5.68 GTDD103 pKa = 2.72 GRR105 pKa = 11.84 GSHH108 pKa = 6.42 RR109 pKa = 11.84 AHH111 pKa = 7.53 RR112 pKa = 11.84 YY113 pKa = 8.09
Molecular weight: 12.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 10.979
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.281
Grimsley 12.676
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.003
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14495
0
14495
6380480
21
9789
440.2
48.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.046 ± 0.022
1.249 ± 0.008
5.678 ± 0.016
6.006 ± 0.023
3.755 ± 0.012
7.108 ± 0.019
2.352 ± 0.011
4.771 ± 0.015
4.803 ± 0.022
8.717 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.009
3.629 ± 0.011
6.165 ± 0.027
3.921 ± 0.013
6.052 ± 0.019
8.113 ± 0.023
6.049 ± 0.027
6.23 ± 0.016
1.51 ± 0.009
2.665 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here