Lachnospiraceae bacterium A10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6UPR6|A0A1H6UPR6_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium A10 OX=1520821 GN=SAMN02910453_1645 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.4FHH4 pKa = 7.11TNRR7 pKa = 11.84LQPKK11 pKa = 9.5RR12 pKa = 11.84FGALFLAASMLTLSACGSDD31 pKa = 3.81SISYY35 pKa = 10.27EE36 pKa = 3.97PLTEE40 pKa = 4.52EE41 pKa = 5.63DD42 pKa = 3.43DD43 pKa = 3.89TVYY46 pKa = 10.83NICIVQSEE54 pKa = 5.01DD55 pKa = 3.32NTEE58 pKa = 4.04LNQIQQGFQDD68 pKa = 3.85ALTDD72 pKa = 4.1LFGEE76 pKa = 4.16DD77 pKa = 3.58HH78 pKa = 7.17VNVTVQVANSEE89 pKa = 4.32TSVDD93 pKa = 4.74DD94 pKa = 3.76INSTYY99 pKa = 8.12TTDD102 pKa = 4.03EE103 pKa = 3.8NTQLIFANGSSALTSAAFYY122 pKa = 10.46TDD124 pKa = 5.22DD125 pKa = 4.12IPVVGAGVIDD135 pKa = 4.3FQSALHH141 pKa = 6.2TPDD144 pKa = 4.48SEE146 pKa = 4.05WDD148 pKa = 3.24AKK150 pKa = 10.46TGTNVTGISSRR161 pKa = 11.84PPIAAQLSLLIEE173 pKa = 4.47ATSDD177 pKa = 3.41LQTVGILYY185 pKa = 10.36CPEE188 pKa = 4.17DD189 pKa = 3.43TDD191 pKa = 5.9AIYY194 pKa = 10.8QNEE197 pKa = 4.1ILEE200 pKa = 5.05DD201 pKa = 3.79YY202 pKa = 10.78LDD204 pKa = 3.56QAGIAWRR211 pKa = 11.84EE212 pKa = 3.7YY213 pKa = 10.15EE214 pKa = 4.17IPSTEE219 pKa = 3.79AAITEE224 pKa = 4.52HH225 pKa = 6.1EE226 pKa = 4.26TSIDD230 pKa = 3.74TEE232 pKa = 4.64TSGSTISGDD241 pKa = 3.26KK242 pKa = 10.62VVANSAKK249 pKa = 9.71EE250 pKa = 3.79GSEE253 pKa = 3.57IDD255 pKa = 3.6VEE257 pKa = 5.17SIGEE261 pKa = 4.29SNVLFGLNSTNSARR275 pKa = 11.84SAKK278 pKa = 9.93ISANWTQDD286 pKa = 2.56INDD289 pKa = 4.11PVSYY293 pKa = 10.84DD294 pKa = 3.32LGSTTEE300 pKa = 4.21EE301 pKa = 3.89IVNYY305 pKa = 10.0AASQSSAFFISANSYY320 pKa = 11.19LDD322 pKa = 3.99DD323 pKa = 4.94QIDD326 pKa = 4.36TIASIANATGTTTVSADD343 pKa = 3.21TTIASKK349 pKa = 9.46TLVSLYY355 pKa = 9.85IDD357 pKa = 5.05PYY359 pKa = 10.66NEE361 pKa = 4.59GYY363 pKa = 10.19SAGKK367 pKa = 8.68LAYY370 pKa = 9.76RR371 pKa = 11.84ILVNGDD377 pKa = 3.44DD378 pKa = 4.25PGSIKK383 pKa = 9.45ITGTNADD390 pKa = 4.78DD391 pKa = 3.67ATKK394 pKa = 10.52LYY396 pKa = 10.46QDD398 pKa = 4.65TIAEE402 pKa = 4.23QFGLTFGKK410 pKa = 10.35SFSEE414 pKa = 4.13VDD416 pKa = 3.7DD417 pKa = 4.17FLNTYY422 pKa = 9.95VPGTNTEE429 pKa = 4.32RR430 pKa = 11.84ISSEE434 pKa = 3.94EE435 pKa = 3.87EE436 pKa = 3.63

Molecular weight: 46.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6S7Y6|A0A1H6S7Y6_9FIRM Carbohydrate diacid regulator OS=Lachnospiraceae bacterium A10 OX=1520821 GN=SAMN02910453_0960 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.55MTYY5 pKa = 8.41QPKK8 pKa = 8.94RR9 pKa = 11.84RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.92VHH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 8.84VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2244

0

2244

749661

37

2935

334.1

37.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.29 ± 0.049

1.298 ± 0.021

6.196 ± 0.041

7.876 ± 0.064

4.147 ± 0.037

6.741 ± 0.041

1.85 ± 0.021

7.501 ± 0.051

6.608 ± 0.047

8.74 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.323 ± 0.03

4.495 ± 0.037

3.003 ± 0.029

3.166 ± 0.026

4.198 ± 0.039

5.939 ± 0.052

5.446 ± 0.054

7.107 ± 0.044

0.818 ± 0.016

4.256 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski