Pueribacillus theae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pueribacillus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1K374|A0A2U1K374_9BACI Squalene--hopene cyclase OS=Pueribacillus theae OX=2171751 GN=shc PE=3 SV=1
MM1 pKa = 7.46SKK3 pKa = 9.18YY4 pKa = 9.45TIIDD8 pKa = 3.5KK9 pKa = 7.94NTCIACGTCGAIAPDD24 pKa = 3.54IYY26 pKa = 11.03DD27 pKa = 3.83YY28 pKa = 11.72DD29 pKa = 5.09DD30 pKa = 5.64DD31 pKa = 6.08GIAYY35 pKa = 9.69AILDD39 pKa = 3.84NNKK42 pKa = 10.35GIVKK46 pKa = 9.86VPEE49 pKa = 4.06EE50 pKa = 4.0FLDD53 pKa = 5.09DD54 pKa = 4.09IEE56 pKa = 4.61EE57 pKa = 4.38ACEE60 pKa = 4.15GCPTDD65 pKa = 3.73SLKK68 pKa = 11.18VADD71 pKa = 4.93KK72 pKa = 11.1PFGNDD77 pKa = 2.28II78 pKa = 4.09

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1JNG5|A0A2U1JNG5_9BACI Chromosome partitioning protein ParB OS=Pueribacillus theae OX=2171751 GN=DCC39_17220 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 8.84VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.22GRR39 pKa = 11.84KK40 pKa = 8.65ILSAA44 pKa = 4.02

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3627

0

3627

1053953

21

2032

290.6

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.058 ± 0.044

0.729 ± 0.011

4.968 ± 0.032

7.83 ± 0.046

4.591 ± 0.03

6.899 ± 0.04

2.058 ± 0.019

7.925 ± 0.039

7.294 ± 0.039

9.712 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.016

4.483 ± 0.027

3.731 ± 0.023

3.523 ± 0.026

4.233 ± 0.028

5.857 ± 0.025

5.194 ± 0.025

6.805 ± 0.034

1.015 ± 0.014

3.353 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski