Streptomyces sp. 844.5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1KR25|A0A3N1KR25_9ACTN Fructoselysine-6-P-deglycase FrlB-like protein OS=Streptomyces sp. 844.5 OX=2485140 GN=EDB18_6418 PE=4 SV=1
MM1 pKa = 7.46GLFTRR6 pKa = 11.84RR7 pKa = 11.84SGFEE11 pKa = 3.75FDD13 pKa = 5.19AVTADD18 pKa = 3.05GVTYY22 pKa = 10.61DD23 pKa = 5.16AVTDD27 pKa = 4.06DD28 pKa = 4.71DD29 pKa = 4.35GVVYY33 pKa = 10.86LEE35 pKa = 5.82GYY37 pKa = 10.5DD38 pKa = 3.16EE39 pKa = 5.92DD40 pKa = 4.11YY41 pKa = 10.94RR42 pKa = 11.84VWIDD46 pKa = 3.14GVIIGDD52 pKa = 3.57YY53 pKa = 9.37WYY55 pKa = 8.8PAHH58 pKa = 6.61TDD60 pKa = 3.02EE61 pKa = 5.56HH62 pKa = 6.96GRR64 pKa = 11.84VYY66 pKa = 10.93LDD68 pKa = 3.35EE69 pKa = 4.81DD70 pKa = 3.65

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1LHJ6|A0A3N1LHJ6_9ACTN Uncharacterized protein OS=Streptomyces sp. 844.5 OX=2485140 GN=EDB18_5837 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84ARR4 pKa = 11.84SSWPSSKK11 pKa = 10.59SSASSCVRR19 pKa = 11.84RR20 pKa = 11.84PRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84LSYY29 pKa = 10.45EE30 pKa = 3.64SWKK33 pKa = 10.58LPSVGPAAPRR43 pKa = 11.84SLGRR47 pKa = 11.84RR48 pKa = 11.84ASRR51 pKa = 11.84HH52 pKa = 5.37PPSPQARR59 pKa = 11.84RR60 pKa = 11.84LRR62 pKa = 11.84PHH64 pKa = 7.09RR65 pKa = 11.84VRR67 pKa = 11.84APRR70 pKa = 11.84APPRR74 pKa = 11.84RR75 pKa = 11.84VPPPLQHH82 pKa = 7.09PLRR85 pKa = 11.84RR86 pKa = 11.84PPPRR90 pKa = 11.84PPHH93 pKa = 6.19RR94 pKa = 11.84GPRR97 pKa = 11.84LPRR100 pKa = 11.84ARR102 pKa = 11.84ARR104 pKa = 11.84LRR106 pKa = 11.84VRR108 pKa = 11.84PLRR111 pKa = 11.84PPSSRR116 pKa = 11.84PRR118 pKa = 11.84PRR120 pKa = 11.84LPRR123 pKa = 11.84RR124 pKa = 11.84PPPRR128 pKa = 11.84PLRR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84LRR135 pKa = 11.84PPRR138 pKa = 11.84PRR140 pKa = 11.84PRR142 pKa = 11.84VRR144 pKa = 11.84LPPVRR149 pKa = 11.84RR150 pKa = 11.84VPVLRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84VPPRR161 pKa = 11.84PRR163 pKa = 11.84APARR167 pKa = 11.84PARR170 pKa = 11.84VRR172 pKa = 11.84VTTPSRR178 pKa = 11.84PATPACRR185 pKa = 11.84DD186 pKa = 3.44RR187 pKa = 11.84APARR191 pKa = 11.84PAPAPSGPPPRR202 pKa = 11.84VRR204 pKa = 11.84AATVRR209 pKa = 11.84LPVALRR215 pKa = 11.84VPVASVRR222 pKa = 11.84PVRR225 pKa = 11.84VATVRR230 pKa = 11.84VLRR233 pKa = 11.84VALRR237 pKa = 11.84VPVVTVRR244 pKa = 11.84PVRR247 pKa = 11.84VATVRR252 pKa = 11.84VLRR255 pKa = 11.84VALRR259 pKa = 11.84VPVVTVRR266 pKa = 11.84PARR269 pKa = 11.84PATVPVLPVGCPVRR283 pKa = 11.84RR284 pKa = 11.84AVLVPVGRR292 pKa = 11.84RR293 pKa = 11.84RR294 pKa = 11.84PVCPARR300 pKa = 11.84TRR302 pKa = 11.84AA303 pKa = 3.35

Molecular weight: 34.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7536

0

7536

2536846

29

7492

336.6

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.786 ± 0.043

0.836 ± 0.008

5.667 ± 0.022

5.058 ± 0.033

2.692 ± 0.016

9.516 ± 0.027

2.276 ± 0.013

3.167 ± 0.017

1.731 ± 0.019

10.689 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.72 ± 0.011

1.941 ± 0.02

6.309 ± 0.029

3.232 ± 0.018

7.576 ± 0.034

5.61 ± 0.025

6.36 ± 0.034

8.277 ± 0.025

1.524 ± 0.013

2.033 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski