Homoserinimonas aerilata
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2633 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A542YHZ8|A0A542YHZ8_9MICO 2-hydroxychromene-2-carboxylate isomerase OS=Homoserinimonas aerilata OX=1162970 GN=FB562_0805 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 10.21 KK3 pKa = 10.43 RR4 pKa = 11.84 FMTSIAVGAVALLGLTACAGSADD27 pKa = 3.82 TGDD30 pKa = 3.89 NGSGDD35 pKa = 4.23 GDD37 pKa = 4.05 SKK39 pKa = 11.61 SLTVGVFNGWPEE51 pKa = 4.14 GEE53 pKa = 4.02 AASYY57 pKa = 9.67 LWKK60 pKa = 10.65 AVLEE64 pKa = 4.25 EE65 pKa = 3.93 KK66 pKa = 10.76 GYY68 pKa = 11.15 DD69 pKa = 3.47 VTLEE73 pKa = 4.02 YY74 pKa = 11.15 ADD76 pKa = 4.77 AGPVFAGLSTGDD88 pKa = 3.42 YY89 pKa = 11.18 DD90 pKa = 4.34 VAFDD94 pKa = 3.27 GWLPLTHH101 pKa = 6.32 QSYY104 pKa = 9.68 MDD106 pKa = 3.64 EE107 pKa = 4.57 YY108 pKa = 11.2 GDD110 pKa = 3.87 SLEE113 pKa = 6.27 DD114 pKa = 3.42 LGSWNDD120 pKa = 3.72 DD121 pKa = 3.02 AVLTVAVNEE130 pKa = 4.38 DD131 pKa = 3.56 APITSLDD138 pKa = 3.68 EE139 pKa = 4.55 LADD142 pKa = 3.87 HH143 pKa = 7.3 ADD145 pKa = 3.39 EE146 pKa = 4.7 FGNRR150 pKa = 11.84 IVGIEE155 pKa = 4.04 PGAGLTKK162 pKa = 10.54 AVGEE166 pKa = 4.21 QTIPTYY172 pKa = 11.03 GLDD175 pKa = 3.22 GMEE178 pKa = 5.1 FLTSSTPAMLSEE190 pKa = 4.83 LSAALDD196 pKa = 3.59 AGEE199 pKa = 4.25 NVAVTLWRR207 pKa = 11.84 PHH209 pKa = 5.02 WAYY212 pKa = 10.82 DD213 pKa = 3.5 EE214 pKa = 4.31 YY215 pKa = 10.64 PIRR218 pKa = 11.84 DD219 pKa = 3.72 LEE221 pKa = 4.46 DD222 pKa = 3.88 PEE224 pKa = 4.53 GTLGASEE231 pKa = 5.43 GIHH234 pKa = 6.25 SIARR238 pKa = 11.84 SGFGDD243 pKa = 3.87 EE244 pKa = 4.31 FSEE247 pKa = 4.19 LAGWIGDD254 pKa = 3.53 WKK256 pKa = 10.79 LDD258 pKa = 3.85 SEE260 pKa = 4.79 LLYY263 pKa = 11.06 SLEE266 pKa = 3.71 NAMYY270 pKa = 10.76 NSGADD275 pKa = 3.22 ASEE278 pKa = 4.47 YY279 pKa = 10.79 EE280 pKa = 4.79 SIVADD285 pKa = 4.69 WIAEE289 pKa = 3.93 NQEE292 pKa = 4.25 YY293 pKa = 11.1 VDD295 pKa = 4.58 GLTSS299 pKa = 3.2
Molecular weight: 31.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.49
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.821
Patrickios 1.202
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.741
Protein with the highest isoelectric point:
>tr|A0A542YA47|A0A542YA47_9MICO HAD superfamily hydrolase (TIGR01490 family) OS=Homoserinimonas aerilata OX=1162970 GN=FB562_2380 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2633
0
2633
851979
30
1985
323.6
34.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.998 ± 0.074
0.476 ± 0.01
5.912 ± 0.041
5.818 ± 0.044
3.236 ± 0.028
8.989 ± 0.044
1.931 ± 0.023
4.918 ± 0.038
2.269 ± 0.036
10.295 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.906 ± 0.017
2.166 ± 0.026
5.118 ± 0.036
2.919 ± 0.027
6.776 ± 0.046
6.148 ± 0.034
5.947 ± 0.037
8.835 ± 0.044
1.4 ± 0.021
1.943 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here