Pectobacterium phage phiTE

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Certrevirus; Pectobacterium virus phiTE

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9L3Y6|K9L3Y6_9CAUD Uncharacterized protein OS=Pectobacterium phage phiTE OX=1116482 GN=phiTE_233 PE=4 SV=1
MM1 pKa = 7.37NPYY4 pKa = 10.73KK5 pKa = 10.18LTLSFITPDD14 pKa = 3.46YY15 pKa = 10.36TSEE18 pKa = 4.14EE19 pKa = 4.28HH20 pKa = 6.69CEE22 pKa = 4.03HH23 pKa = 6.8YY24 pKa = 7.77PTLQDD29 pKa = 4.58ALDD32 pKa = 4.64DD33 pKa = 4.72ADD35 pKa = 5.53DD36 pKa = 4.35LAFSTVGFPLEE47 pKa = 4.04DD48 pKa = 3.3WQNVDD53 pKa = 3.94FGEE56 pKa = 4.57GNHH59 pKa = 6.68LYY61 pKa = 10.5SFYY64 pKa = 10.6PNEE67 pKa = 3.69VDD69 pKa = 2.87KK70 pKa = 11.11TSRR73 pKa = 11.84YY74 pKa = 9.65KK75 pKa = 10.77LAILKK80 pKa = 10.59EE81 pKa = 4.4EE82 pKa = 4.24VV83 pKa = 2.98

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9L4A5|K9L4A5_9CAUD Uncharacterized protein OS=Pectobacterium phage phiTE OX=1116482 GN=phiTE_117 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 6.08CLPKK6 pKa = 10.56CYY8 pKa = 9.9DD9 pKa = 3.62SPRR12 pKa = 11.84VPCQWKK18 pKa = 8.61KK19 pKa = 9.3DD20 pKa = 3.47QRR22 pKa = 11.84VRR24 pKa = 11.84ILRR27 pKa = 11.84GKK29 pKa = 9.78NRR31 pKa = 11.84GRR33 pKa = 11.84TALVSYY39 pKa = 7.74EE40 pKa = 4.28TEE42 pKa = 3.77PHH44 pKa = 5.92HH45 pKa = 7.41LSRR48 pKa = 11.84QSGAPIEE55 pKa = 4.28KK56 pKa = 9.83LRR58 pKa = 11.84SSSVVAYY65 pKa = 7.11YY66 pKa = 9.46QRR68 pKa = 11.84KK69 pKa = 8.68YY70 pKa = 9.88LTRR73 pKa = 11.84CPSGGRR79 pKa = 11.84LGLSIGGGVAMARR92 pKa = 11.84KK93 pKa = 9.21YY94 pKa = 10.16LWSGRR99 pKa = 11.84KK100 pKa = 9.12CRR102 pKa = 11.84DD103 pKa = 3.44FWCTRR108 pKa = 11.84YY109 pKa = 8.46TSSRR113 pKa = 11.84RR114 pKa = 11.84PRR116 pKa = 11.84VYY118 pKa = 9.99EE119 pKa = 3.83VGEE122 pKa = 3.72FSYY125 pKa = 9.87WASSRR130 pKa = 11.84GEE132 pKa = 3.88VVRR135 pKa = 11.84QVIYY139 pKa = 10.62RR140 pKa = 11.84RR141 pKa = 11.84TAIHH145 pKa = 6.75GLRR148 pKa = 11.84EE149 pKa = 4.08EE150 pKa = 4.17EE151 pKa = 4.45VKK153 pKa = 10.88DD154 pKa = 3.17ILQTSRR160 pKa = 11.84RR161 pKa = 11.84NCDD164 pKa = 2.76DD165 pKa = 2.25WHH167 pKa = 5.29TRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84EE172 pKa = 3.97DD173 pKa = 3.25GLLDD177 pKa = 3.83LFHH180 pKa = 7.32IKK182 pKa = 9.95RR183 pKa = 11.84VRR185 pKa = 11.84DD186 pKa = 3.53QIISRR191 pKa = 11.84TYY193 pKa = 10.15GRR195 pKa = 11.84RR196 pKa = 11.84DD197 pKa = 3.41AII199 pKa = 4.02

Molecular weight: 23.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

242

0

242

42188

32

1099

174.3

19.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.061 ± 0.207

1.304 ± 0.108

6.559 ± 0.146

6.993 ± 0.21

4.079 ± 0.123

8.002 ± 0.333

2.041 ± 0.1

5.49 ± 0.124

6.77 ± 0.196

7.806 ± 0.14

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.908 ± 0.094

4.252 ± 0.12

3.982 ± 0.148

3.404 ± 0.111

5.435 ± 0.169

5.777 ± 0.149

5.634 ± 0.211

7.116 ± 0.141

1.576 ± 0.064

3.812 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski