Amino acid dipepetide frequency for Pectobacterium phage phiTE

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.4AlaAla: 6.4 ± 0.527
0.901AlaCys: 0.901 ± 0.172
3.864AlaAsp: 3.864 ± 0.327
4.599AlaGlu: 4.599 ± 0.346
2.702AlaPhe: 2.702 ± 0.234
5.997AlaGly: 5.997 ± 0.485
1.825AlaHis: 1.825 ± 0.199
4.03AlaIle: 4.03 ± 0.289
4.267AlaLys: 4.267 ± 0.309
5.618AlaLeu: 5.618 ± 0.341
2.418AlaMet: 2.418 ± 0.221
2.797AlaAsn: 2.797 ± 0.238
2.299AlaPro: 2.299 ± 0.26
3.058AlaGln: 3.058 ± 0.325
3.793AlaArg: 3.793 ± 0.322
3.484AlaSer: 3.484 ± 0.325
3.911AlaThr: 3.911 ± 0.451
4.765AlaVal: 4.765 ± 0.303
1.233AlaTrp: 1.233 ± 0.167
2.655AlaTyr: 2.655 ± 0.277
0.0AlaXaa: 0.0 ± 0.0
Cys
0.711CysAla: 0.711 ± 0.104
0.237CysCys: 0.237 ± 0.074
0.877CysAsp: 0.877 ± 0.144
0.972CysGlu: 0.972 ± 0.139
0.664CysPhe: 0.664 ± 0.142
1.493CysGly: 1.493 ± 0.187
0.379CysHis: 0.379 ± 0.085
0.308CysIle: 0.308 ± 0.075
0.759CysLys: 0.759 ± 0.145
1.067CysLeu: 1.067 ± 0.185
0.356CysMet: 0.356 ± 0.074
0.616CysAsn: 0.616 ± 0.138
0.83CysPro: 0.83 ± 0.171
0.427CysGln: 0.427 ± 0.101
0.901CysArg: 0.901 ± 0.163
0.735CysSer: 0.735 ± 0.189
0.427CysThr: 0.427 ± 0.106
0.521CysVal: 0.521 ± 0.094
0.166CysTrp: 0.166 ± 0.071
0.593CysTyr: 0.593 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.243AspAla: 4.243 ± 0.33
0.498AspCys: 0.498 ± 0.112
4.196AspAsp: 4.196 ± 0.406
3.887AspGlu: 3.887 ± 0.319
3.271AspPhe: 3.271 ± 0.29
5.452AspGly: 5.452 ± 0.415
1.422AspHis: 1.422 ± 0.193
3.722AspIle: 3.722 ± 0.337
3.698AspLys: 3.698 ± 0.363
6.685AspLeu: 6.685 ± 0.34
2.37AspMet: 2.37 ± 0.227
3.034AspAsn: 3.034 ± 0.257
2.963AspPro: 2.963 ± 0.3
2.442AspGln: 2.442 ± 0.215
3.082AspArg: 3.082 ± 0.303
3.176AspSer: 3.176 ± 0.305
3.2AspThr: 3.2 ± 0.412
4.29AspVal: 4.29 ± 0.32
1.351AspTrp: 1.351 ± 0.182
2.607AspTyr: 2.607 ± 0.229
0.0AspXaa: 0.0 ± 0.0
Glu
5.12GluAla: 5.12 ± 0.363
0.664GluCys: 0.664 ± 0.121
5.191GluAsp: 5.191 ± 0.35
5.784GluGlu: 5.784 ± 0.416
2.987GluPhe: 2.987 ± 0.258
5.523GluGly: 5.523 ± 0.409
1.541GluHis: 1.541 ± 0.22
4.338GluIle: 4.338 ± 0.295
5.096GluLys: 5.096 ± 0.374
5.286GluLeu: 5.286 ± 0.33
2.086GluMet: 2.086 ± 0.195
2.679GluAsn: 2.679 ± 0.191
1.683GluPro: 1.683 ± 0.207
2.631GluGln: 2.631 ± 0.26
3.437GluArg: 3.437 ± 0.333
3.295GluSer: 3.295 ± 0.266
3.532GluThr: 3.532 ± 0.253
5.523GluVal: 5.523 ± 0.377
1.209GluTrp: 1.209 ± 0.175
2.323GluTyr: 2.323 ± 0.266
0.0GluXaa: 0.0 ± 0.0
Phe
2.489PheAla: 2.489 ± 0.252
0.64PheCys: 0.64 ± 0.142
2.963PheAsp: 2.963 ± 0.292
2.939PheGlu: 2.939 ± 0.289
1.873PhePhe: 1.873 ± 0.21
3.271PheGly: 3.271 ± 0.326
0.782PheHis: 0.782 ± 0.148
2.276PheIle: 2.276 ± 0.209
2.679PheLys: 2.679 ± 0.282
3.2PheLeu: 3.2 ± 0.289
0.996PheMet: 0.996 ± 0.15
2.015PheAsn: 2.015 ± 0.201
1.541PhePro: 1.541 ± 0.18
1.138PheGln: 1.138 ± 0.174
1.446PheArg: 1.446 ± 0.166
2.536PheSer: 2.536 ± 0.251
2.394PheThr: 2.394 ± 0.233
2.939PheVal: 2.939 ± 0.259
0.877PheTrp: 0.877 ± 0.154
1.802PheTyr: 1.802 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
5.049GlyAla: 5.049 ± 0.578
0.996GlyCys: 0.996 ± 0.164
5.191GlyAsp: 5.191 ± 0.8
5.191GlyGlu: 5.191 ± 0.38
3.342GlyPhe: 3.342 ± 0.352
5.618GlyGly: 5.618 ± 0.455
1.304GlyHis: 1.304 ± 0.164
4.077GlyIle: 4.077 ± 0.329
6.827GlyLys: 6.827 ± 0.392
5.95GlyLeu: 5.95 ± 0.449
2.442GlyMet: 2.442 ± 0.257
3.224GlyAsn: 3.224 ± 0.269
2.868GlyPro: 2.868 ± 1.004
2.584GlyGln: 2.584 ± 0.271
4.29GlyArg: 4.29 ± 0.293
5.002GlySer: 5.002 ± 0.314
5.073GlyThr: 5.073 ± 0.544
5.736GlyVal: 5.736 ± 0.347
1.09GlyTrp: 1.09 ± 0.169
4.172GlyTyr: 4.172 ± 0.273
0.0GlyXaa: 0.0 ± 0.0
His
0.924HisAla: 0.924 ± 0.145
0.332HisCys: 0.332 ± 0.079
1.256HisAsp: 1.256 ± 0.197
1.256HisGlu: 1.256 ± 0.18
0.877HisPhe: 0.877 ± 0.147
1.659HisGly: 1.659 ± 0.194
0.427HisHis: 0.427 ± 0.081
1.304HisIle: 1.304 ± 0.165
1.375HisLys: 1.375 ± 0.186
1.778HisLeu: 1.778 ± 0.188
0.64HisMet: 0.64 ± 0.125
1.161HisAsn: 1.161 ± 0.168
1.043HisPro: 1.043 ± 0.161
0.474HisGln: 0.474 ± 0.089
1.47HisArg: 1.47 ± 0.172
0.83HisSer: 0.83 ± 0.136
1.185HisThr: 1.185 ± 0.168
1.327HisVal: 1.327 ± 0.155
0.356HisTrp: 0.356 ± 0.09
0.735HisTyr: 0.735 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
3.508IleAla: 3.508 ± 0.303
0.735IleCys: 0.735 ± 0.134
4.101IleAsp: 4.101 ± 0.242
4.077IleGlu: 4.077 ± 0.346
2.039IlePhe: 2.039 ± 0.222
4.077IleGly: 4.077 ± 0.372
1.256IleHis: 1.256 ± 0.202
2.702IleIle: 2.702 ± 0.25
3.603IleLys: 3.603 ± 0.33
3.959IleLeu: 3.959 ± 0.307
1.47IleMet: 1.47 ± 0.206
2.37IleAsn: 2.37 ± 0.201
2.418IlePro: 2.418 ± 0.226
1.707IleGln: 1.707 ± 0.194
3.864IleArg: 3.864 ± 0.279
3.319IleSer: 3.319 ± 0.253
3.39IleThr: 3.39 ± 0.31
3.722IleVal: 3.722 ± 0.328
0.664IleTrp: 0.664 ± 0.118
1.92IleTyr: 1.92 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
5.736LysAla: 5.736 ± 0.486
0.972LysCys: 0.972 ± 0.156
4.48LysAsp: 4.48 ± 0.367
5.025LysGlu: 5.025 ± 0.42
2.75LysPhe: 2.75 ± 0.242
6.045LysGly: 6.045 ± 0.58
1.28LysHis: 1.28 ± 0.187
3.887LysIle: 3.887 ± 0.308
4.67LysLys: 4.67 ± 0.386
4.267LysLeu: 4.267 ± 0.343
2.394LysMet: 2.394 ± 0.257
2.204LysAsn: 2.204 ± 0.216
2.607LysPro: 2.607 ± 0.304
2.489LysGln: 2.489 ± 0.257
3.816LysArg: 3.816 ± 0.315
3.579LysSer: 3.579 ± 0.327
3.627LysThr: 3.627 ± 0.288
4.456LysVal: 4.456 ± 0.261
1.019LysTrp: 1.019 ± 0.14
2.37LysTyr: 2.37 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
5.855LeuAla: 5.855 ± 0.339
1.138LeuCys: 1.138 ± 0.169
5.167LeuAsp: 5.167 ± 0.352
5.926LeuGlu: 5.926 ± 0.383
2.892LeuPhe: 2.892 ± 0.268
5.286LeuGly: 5.286 ± 0.353
1.778LeuHis: 1.778 ± 0.255
4.362LeuIle: 4.362 ± 0.315
5.191LeuLys: 5.191 ± 0.384
5.144LeuLeu: 5.144 ± 0.408
2.37LeuMet: 2.37 ± 0.262
3.864LeuAsn: 3.864 ± 0.282
3.864LeuPro: 3.864 ± 0.3
2.11LeuGln: 2.11 ± 0.211
4.243LeuArg: 4.243 ± 0.342
5.286LeuSer: 5.286 ± 0.365
4.67LeuThr: 4.67 ± 0.339
4.622LeuVal: 4.622 ± 0.313
1.209LeuTrp: 1.209 ± 0.164
3.082LeuTyr: 3.082 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
2.679MetAla: 2.679 ± 0.251
0.45MetCys: 0.45 ± 0.104
1.375MetAsp: 1.375 ± 0.205
2.062MetGlu: 2.062 ± 0.233
1.47MetPhe: 1.47 ± 0.208
1.588MetGly: 1.588 ± 0.195
0.474MetHis: 0.474 ± 0.108
1.802MetIle: 1.802 ± 0.223
2.584MetLys: 2.584 ± 0.265
1.873MetLeu: 1.873 ± 0.213
0.569MetMet: 0.569 ± 0.12
1.138MetAsn: 1.138 ± 0.147
1.043MetPro: 1.043 ± 0.139
1.09MetGln: 1.09 ± 0.153
1.493MetArg: 1.493 ± 0.177
2.157MetSer: 2.157 ± 0.23
2.039MetThr: 2.039 ± 0.214
2.015MetVal: 2.015 ± 0.221
0.308MetTrp: 0.308 ± 0.078
0.877MetTyr: 0.877 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.129AsnAla: 3.129 ± 0.281
0.498AsnCys: 0.498 ± 0.116
2.086AsnAsp: 2.086 ± 0.192
2.276AsnGlu: 2.276 ± 0.23
1.399AsnPhe: 1.399 ± 0.171
3.911AsnGly: 3.911 ± 0.271
0.759AsnHis: 0.759 ± 0.124
2.418AsnIle: 2.418 ± 0.265
2.797AsnLys: 2.797 ± 0.24
3.959AsnLeu: 3.959 ± 0.314
0.972AsnMet: 0.972 ± 0.167
2.11AsnAsn: 2.11 ± 0.264
1.991AsnPro: 1.991 ± 0.214
1.636AsnGln: 1.636 ± 0.196
2.418AsnArg: 2.418 ± 0.257
2.37AsnSer: 2.37 ± 0.201
2.75AsnThr: 2.75 ± 0.267
2.892AsnVal: 2.892 ± 0.274
0.64AsnTrp: 0.64 ± 0.118
1.517AsnTyr: 1.517 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
2.939ProAla: 2.939 ± 0.355
0.664ProCys: 0.664 ± 0.118
3.105ProAsp: 3.105 ± 0.331
3.769ProGlu: 3.769 ± 0.359
1.446ProPhe: 1.446 ± 0.191
2.892ProGly: 2.892 ± 0.271
0.877ProHis: 0.877 ± 0.147
1.754ProIle: 1.754 ± 0.201
2.631ProLys: 2.631 ± 0.293
2.631ProLeu: 2.631 ± 0.249
1.067ProMet: 1.067 ± 0.135
1.375ProAsn: 1.375 ± 0.169
1.47ProPro: 1.47 ± 0.231
1.517ProGln: 1.517 ± 0.307
1.849ProArg: 1.849 ± 0.164
2.228ProSer: 2.228 ± 0.188
2.489ProThr: 2.489 ± 0.292
3.129ProVal: 3.129 ± 0.267
0.64ProTrp: 0.64 ± 0.111
1.351ProTyr: 1.351 ± 0.176
0.0ProXaa: 0.0 ± 0.0
Gln
2.465GlnAla: 2.465 ± 0.239
0.308GlnCys: 0.308 ± 0.083
1.707GlnAsp: 1.707 ± 0.193
2.299GlnGlu: 2.299 ± 0.249
1.446GlnPhe: 1.446 ± 0.156
2.536GlnGly: 2.536 ± 0.579
0.498GlnHis: 0.498 ± 0.115
2.418GlnIle: 2.418 ± 0.23
1.944GlnLys: 1.944 ± 0.224
2.536GlnLeu: 2.536 ± 0.205
1.161GlnMet: 1.161 ± 0.185
1.896GlnAsn: 1.896 ± 0.188
1.185GlnPro: 1.185 ± 0.158
1.138GlnGln: 1.138 ± 0.191
1.778GlnArg: 1.778 ± 0.212
1.659GlnSer: 1.659 ± 0.178
1.873GlnThr: 1.873 ± 0.203
2.726GlnVal: 2.726 ± 0.266
0.687GlnTrp: 0.687 ± 0.124
1.778GlnTyr: 1.778 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
3.579ArgAla: 3.579 ± 0.314
0.877ArgCys: 0.877 ± 0.169
3.65ArgAsp: 3.65 ± 0.292
3.84ArgGlu: 3.84 ± 0.315
2.039ArgPhe: 2.039 ± 0.256
4.29ArgGly: 4.29 ± 0.292
1.067ArgHis: 1.067 ± 0.184
2.655ArgIle: 2.655 ± 0.286
4.409ArgLys: 4.409 ± 0.359
4.504ArgLeu: 4.504 ± 0.325
1.683ArgMet: 1.683 ± 0.236
2.086ArgAsn: 2.086 ± 0.225
1.92ArgPro: 1.92 ± 0.186
1.991ArgGln: 1.991 ± 0.219
3.271ArgArg: 3.271 ± 0.339
3.176ArgSer: 3.176 ± 0.226
2.347ArgThr: 2.347 ± 0.244
4.599ArgVal: 4.599 ± 0.339
0.735ArgTrp: 0.735 ± 0.151
1.636ArgTyr: 1.636 ± 0.162
0.0ArgXaa: 0.0 ± 0.0
Ser
3.603SerAla: 3.603 ± 0.324
0.782SerCys: 0.782 ± 0.172
4.101SerAsp: 4.101 ± 0.316
3.579SerGlu: 3.579 ± 0.288
2.062SerPhe: 2.062 ± 0.226
5.215SerGly: 5.215 ± 0.324
0.853SerHis: 0.853 ± 0.157
2.679SerIle: 2.679 ± 0.254
3.556SerLys: 3.556 ± 0.278
4.907SerLeu: 4.907 ± 0.33
1.564SerMet: 1.564 ± 0.177
2.039SerAsn: 2.039 ± 0.244
2.726SerPro: 2.726 ± 0.239
1.802SerGln: 1.802 ± 0.217
3.342SerArg: 3.342 ± 0.316
4.148SerSer: 4.148 ± 0.579
3.224SerThr: 3.224 ± 0.338
4.267SerVal: 4.267 ± 0.329
1.043SerTrp: 1.043 ± 0.148
2.276SerTyr: 2.276 ± 0.247
0.0SerXaa: 0.0 ± 0.0
Thr
3.579ThrAla: 3.579 ± 0.315
0.64ThrCys: 0.64 ± 0.132
3.271ThrAsp: 3.271 ± 0.292
3.579ThrGlu: 3.579 ± 0.347
2.607ThrPhe: 2.607 ± 0.275
6.353ThrGly: 6.353 ± 1.003
1.28ThrHis: 1.28 ± 0.189
3.342ThrIle: 3.342 ± 0.325
3.508ThrLys: 3.508 ± 0.238
4.765ThrLeu: 4.765 ± 0.311
1.327ThrMet: 1.327 ± 0.157
2.37ThrAsn: 2.37 ± 0.242
2.513ThrPro: 2.513 ± 0.288
1.564ThrGln: 1.564 ± 0.176
2.916ThrArg: 2.916 ± 0.263
2.939ThrSer: 2.939 ± 0.256
2.916ThrThr: 2.916 ± 0.351
4.362ThrVal: 4.362 ± 0.43
0.735ThrTrp: 0.735 ± 0.131
1.778ThrTyr: 1.778 ± 0.21
0.0ThrXaa: 0.0 ± 0.0
Val
5.025ValAla: 5.025 ± 0.363
0.877ValCys: 0.877 ± 0.17
5.025ValAsp: 5.025 ± 0.343
5.31ValGlu: 5.31 ± 0.358
2.773ValPhe: 2.773 ± 0.251
4.812ValGly: 4.812 ± 0.343
1.185ValHis: 1.185 ± 0.167
4.243ValIle: 4.243 ± 0.277
5.096ValLys: 5.096 ± 0.33
4.907ValLeu: 4.907 ± 0.381
2.133ValMet: 2.133 ± 0.234
3.39ValAsn: 3.39 ± 0.333
2.773ValPro: 2.773 ± 0.261
2.299ValGln: 2.299 ± 0.23
3.532ValArg: 3.532 ± 0.323
4.504ValSer: 4.504 ± 0.335
4.314ValThr: 4.314 ± 0.302
5.31ValVal: 5.31 ± 0.39
0.996ValTrp: 0.996 ± 0.164
2.655ValTyr: 2.655 ± 0.237
0.0ValXaa: 0.0 ± 0.0
Trp
1.28TrpAla: 1.28 ± 0.184
0.19TrpCys: 0.19 ± 0.069
1.256TrpAsp: 1.256 ± 0.166
1.209TrpGlu: 1.209 ± 0.154
0.759TrpPhe: 0.759 ± 0.114
0.853TrpGly: 0.853 ± 0.131
0.308TrpHis: 0.308 ± 0.078
0.806TrpIle: 0.806 ± 0.132
1.161TrpLys: 1.161 ± 0.181
1.09TrpLeu: 1.09 ± 0.173
0.45TrpMet: 0.45 ± 0.099
0.569TrpAsn: 0.569 ± 0.112
0.545TrpPro: 0.545 ± 0.115
0.593TrpGln: 0.593 ± 0.116
0.972TrpArg: 0.972 ± 0.131
1.067TrpSer: 1.067 ± 0.147
0.806TrpThr: 0.806 ± 0.133
0.901TrpVal: 0.901 ± 0.164
0.261TrpTrp: 0.261 ± 0.099
0.687TrpTyr: 0.687 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.299TyrAla: 2.299 ± 0.221
0.64TyrCys: 0.64 ± 0.12
2.726TyrAsp: 2.726 ± 0.242
2.347TyrGlu: 2.347 ± 0.245
1.399TyrPhe: 1.399 ± 0.196
3.153TyrGly: 3.153 ± 0.267
1.114TyrHis: 1.114 ± 0.155
1.778TyrIle: 1.778 ± 0.211
1.849TyrLys: 1.849 ± 0.202
3.935TyrLeu: 3.935 ± 0.305
0.593TyrMet: 0.593 ± 0.119
1.588TyrAsn: 1.588 ± 0.211
1.541TyrPro: 1.541 ± 0.211
1.351TyrGln: 1.351 ± 0.185
2.442TyrArg: 2.442 ± 0.262
2.276TyrSer: 2.276 ± 0.269
2.181TyrThr: 2.181 ± 0.244
3.058TyrVal: 3.058 ± 0.267
0.545TyrTrp: 0.545 ± 0.132
1.304TyrTyr: 1.304 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 242 proteins (42188 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski