Vibrio phage VPMS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kafunavirus; unclassified Kafunavirus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4T7Z0|S4T7Z0_9CAUD Head completion protein OS=Vibrio phage VPMS1 OX=1233488 GN=MS_007 PE=4 SV=1
MM1 pKa = 7.0TEE3 pKa = 4.98LYY5 pKa = 10.37IFDD8 pKa = 4.59HH9 pKa = 6.71VSDD12 pKa = 3.92SLVEE16 pKa = 4.18EE17 pKa = 4.64VACYY21 pKa = 10.11APCICSNNRR30 pKa = 11.84VVLFKK35 pKa = 10.91EE36 pKa = 4.27VFTDD40 pKa = 4.62LPCDD44 pKa = 3.73VQEE47 pKa = 5.22LLLEE51 pKa = 4.48CEE53 pKa = 4.87LIEE56 pKa = 4.98INN58 pKa = 4.74

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4T7F1|S4T7F1_9CAUD Uncharacterized protein OS=Vibrio phage VPMS1 OX=1233488 GN=MS_020 PE=4 SV=1
MM1 pKa = 7.73LYY3 pKa = 10.06FHH5 pKa = 6.95NFSSSATRR13 pKa = 11.84NSKK16 pKa = 9.23TSSLDD21 pKa = 3.35FASVLVIRR29 pKa = 11.84VASTIVRR36 pKa = 11.84RR37 pKa = 11.84LSMILTASYY46 pKa = 9.65WPLPNQLRR54 pKa = 11.84TAKK57 pKa = 10.42SKK59 pKa = 10.37VNSVQVILASISTPMTLL76 pKa = 4.36

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12916

30

961

243.7

27.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.215 ± 0.43

1.092 ± 0.141

6.364 ± 0.201

7.162 ± 0.229

3.577 ± 0.209

6.682 ± 0.363

1.734 ± 0.213

5.218 ± 0.151

6.651 ± 0.285

8.764 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.161

4.7 ± 0.262

3.608 ± 0.168

4.057 ± 0.173

4.382 ± 0.272

6.449 ± 0.234

6.31 ± 0.288

6.867 ± 0.196

1.324 ± 0.103

4.057 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski