Amino acid dipepetide frequency for Vibrio phage VPMS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.433AlaAla: 7.433 ± 1.05
0.465AlaCys: 0.465 ± 0.193
4.955AlaAsp: 4.955 ± 0.688
6.349AlaGlu: 6.349 ± 0.955
1.936AlaPhe: 1.936 ± 0.338
4.336AlaGly: 4.336 ± 0.631
1.239AlaHis: 1.239 ± 0.292
5.575AlaIle: 5.575 ± 0.765
5.575AlaLys: 5.575 ± 0.894
6.969AlaLeu: 6.969 ± 0.969
2.633AlaMet: 2.633 ± 0.42
4.104AlaAsn: 4.104 ± 0.572
3.097AlaPro: 3.097 ± 0.561
3.02AlaGln: 3.02 ± 0.586
4.259AlaArg: 4.259 ± 0.699
5.575AlaSer: 5.575 ± 0.756
5.575AlaThr: 5.575 ± 0.756
4.723AlaVal: 4.723 ± 0.662
1.549AlaTrp: 1.549 ± 0.407
2.787AlaTyr: 2.787 ± 0.407
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.207
0.155CysCys: 0.155 ± 0.113
0.697CysAsp: 0.697 ± 0.266
1.084CysGlu: 1.084 ± 0.261
0.465CysPhe: 0.465 ± 0.198
0.697CysGly: 0.697 ± 0.244
0.155CysHis: 0.155 ± 0.15
0.465CysIle: 0.465 ± 0.223
0.697CysLys: 0.697 ± 0.246
1.471CysLeu: 1.471 ± 0.335
0.465CysMet: 0.465 ± 0.186
0.387CysAsn: 0.387 ± 0.199
0.31CysPro: 0.31 ± 0.142
0.155CysGln: 0.155 ± 0.104
0.542CysArg: 0.542 ± 0.216
0.697CysSer: 0.697 ± 0.268
0.387CysThr: 0.387 ± 0.135
0.542CysVal: 0.542 ± 0.193
0.232CysTrp: 0.232 ± 0.144
0.697CysTyr: 0.697 ± 0.312
0.0CysXaa: 0.0 ± 0.0
Asp
4.568AspAla: 4.568 ± 0.586
0.929AspCys: 0.929 ± 0.312
3.407AspAsp: 3.407 ± 0.661
5.962AspGlu: 5.962 ± 0.702
3.175AspPhe: 3.175 ± 0.569
4.723AspGly: 4.723 ± 0.529
0.774AspHis: 0.774 ± 0.253
4.181AspIle: 4.181 ± 0.596
4.646AspLys: 4.646 ± 0.705
5.652AspLeu: 5.652 ± 0.575
1.626AspMet: 1.626 ± 0.29
2.245AspAsn: 2.245 ± 0.459
2.865AspPro: 2.865 ± 0.387
2.555AspGln: 2.555 ± 0.419
1.626AspArg: 1.626 ± 0.332
4.491AspSer: 4.491 ± 0.567
3.407AspThr: 3.407 ± 0.398
3.717AspVal: 3.717 ± 0.456
0.542AspTrp: 0.542 ± 0.163
2.555AspTyr: 2.555 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
6.969GluAla: 6.969 ± 0.808
1.239GluCys: 1.239 ± 0.307
4.413GluAsp: 4.413 ± 0.602
5.42GluGlu: 5.42 ± 0.833
2.71GluPhe: 2.71 ± 0.373
5.033GluGly: 5.033 ± 0.566
1.549GluHis: 1.549 ± 0.317
2.787GluIle: 2.787 ± 0.46
3.097GluLys: 3.097 ± 0.383
7.975GluLeu: 7.975 ± 0.963
1.936GluMet: 1.936 ± 0.315
2.555GluAsn: 2.555 ± 0.643
1.471GluPro: 1.471 ± 0.397
3.407GluGln: 3.407 ± 0.514
3.175GluArg: 3.175 ± 0.48
2.787GluSer: 2.787 ± 0.337
4.491GluThr: 4.491 ± 0.619
5.497GluVal: 5.497 ± 0.498
0.852GluTrp: 0.852 ± 0.209
4.181GluTyr: 4.181 ± 0.601
0.0GluXaa: 0.0 ± 0.0
Phe
1.394PheAla: 1.394 ± 0.39
0.31PheCys: 0.31 ± 0.145
2.478PheAsp: 2.478 ± 0.494
3.329PheGlu: 3.329 ± 0.569
0.852PhePhe: 0.852 ± 0.234
3.252PheGly: 3.252 ± 0.576
0.619PheHis: 0.619 ± 0.242
2.168PheIle: 2.168 ± 0.402
3.175PheLys: 3.175 ± 0.453
3.175PheLeu: 3.175 ± 0.476
0.774PheMet: 0.774 ± 0.235
2.71PheAsn: 2.71 ± 0.487
0.929PhePro: 0.929 ± 0.188
1.007PheGln: 1.007 ± 0.259
1.394PheArg: 1.394 ± 0.404
2.168PheSer: 2.168 ± 0.342
2.478PheThr: 2.478 ± 0.478
2.013PheVal: 2.013 ± 0.424
0.387PheTrp: 0.387 ± 0.191
1.161PheTyr: 1.161 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
4.955GlyAla: 4.955 ± 0.635
0.387GlyCys: 0.387 ± 0.175
4.336GlyAsp: 4.336 ± 0.504
4.723GlyGlu: 4.723 ± 0.69
1.858GlyPhe: 1.858 ± 0.422
3.949GlyGly: 3.949 ± 0.882
0.774GlyHis: 0.774 ± 0.238
4.491GlyIle: 4.491 ± 0.464
5.42GlyLys: 5.42 ± 0.709
4.026GlyLeu: 4.026 ± 0.582
1.703GlyMet: 1.703 ± 0.376
3.329GlyAsn: 3.329 ± 0.503
1.703GlyPro: 1.703 ± 0.345
2.71GlyGln: 2.71 ± 0.557
3.02GlyArg: 3.02 ± 0.458
5.497GlySer: 5.497 ± 0.848
4.955GlyThr: 4.955 ± 0.744
4.646GlyVal: 4.646 ± 0.562
1.316GlyTrp: 1.316 ± 0.316
3.02GlyTyr: 3.02 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
1.549HisAla: 1.549 ± 0.387
0.232HisCys: 0.232 ± 0.122
0.465HisAsp: 0.465 ± 0.196
0.929HisGlu: 0.929 ± 0.29
0.465HisPhe: 0.465 ± 0.211
1.084HisGly: 1.084 ± 0.297
0.155HisHis: 0.155 ± 0.165
0.774HisIle: 0.774 ± 0.208
1.316HisLys: 1.316 ± 0.278
1.626HisLeu: 1.626 ± 0.468
0.542HisMet: 0.542 ± 0.202
1.161HisAsn: 1.161 ± 0.271
0.697HisPro: 0.697 ± 0.203
0.697HisGln: 0.697 ± 0.312
0.774HisArg: 0.774 ± 0.227
0.852HisSer: 0.852 ± 0.187
1.316HisThr: 1.316 ± 0.342
1.316HisVal: 1.316 ± 0.309
0.077HisTrp: 0.077 ± 0.078
1.316HisTyr: 1.316 ± 0.358
0.0HisXaa: 0.0 ± 0.0
Ile
4.413IleAla: 4.413 ± 0.618
0.929IleCys: 0.929 ± 0.219
3.871IleAsp: 3.871 ± 0.356
3.871IleGlu: 3.871 ± 0.492
1.007IlePhe: 1.007 ± 0.265
2.942IleGly: 2.942 ± 0.376
0.697IleHis: 0.697 ± 0.252
2.4IleIle: 2.4 ± 0.412
3.717IleLys: 3.717 ± 0.443
3.717IleLeu: 3.717 ± 0.536
1.703IleMet: 1.703 ± 0.307
2.478IleAsn: 2.478 ± 0.375
1.703IlePro: 1.703 ± 0.352
2.323IleGln: 2.323 ± 0.479
2.787IleArg: 2.787 ± 0.52
4.104IleSer: 4.104 ± 0.716
3.717IleThr: 3.717 ± 0.517
2.942IleVal: 2.942 ± 0.653
0.542IleTrp: 0.542 ± 0.188
2.323IleTyr: 2.323 ± 0.454
0.0IleXaa: 0.0 ± 0.0
Lys
5.885LysAla: 5.885 ± 0.844
0.697LysCys: 0.697 ± 0.212
4.491LysAsp: 4.491 ± 0.693
4.723LysGlu: 4.723 ± 0.535
2.091LysPhe: 2.091 ± 0.386
4.181LysGly: 4.181 ± 0.553
1.239LysHis: 1.239 ± 0.422
3.407LysIle: 3.407 ± 0.481
3.562LysLys: 3.562 ± 0.674
7.433LysLeu: 7.433 ± 0.69
1.471LysMet: 1.471 ± 0.309
1.316LysAsn: 1.316 ± 0.297
3.02LysPro: 3.02 ± 0.536
2.478LysGln: 2.478 ± 0.46
2.787LysArg: 2.787 ± 0.43
3.949LysSer: 3.949 ± 0.462
4.336LysThr: 4.336 ± 0.469
5.265LysVal: 5.265 ± 0.543
1.239LysTrp: 1.239 ± 0.333
2.942LysTyr: 2.942 ± 0.387
0.0LysXaa: 0.0 ± 0.0
Leu
8.208LeuAla: 8.208 ± 0.79
0.619LeuCys: 0.619 ± 0.27
6.581LeuAsp: 6.581 ± 0.612
7.201LeuGlu: 7.201 ± 0.685
2.865LeuPhe: 2.865 ± 0.398
5.188LeuGly: 5.188 ± 0.476
2.013LeuHis: 2.013 ± 0.411
3.717LeuIle: 3.717 ± 0.549
5.188LeuLys: 5.188 ± 0.62
6.039LeuLeu: 6.039 ± 0.934
2.865LeuMet: 2.865 ± 0.468
3.717LeuAsn: 3.717 ± 0.484
3.794LeuPro: 3.794 ± 0.57
3.639LeuGln: 3.639 ± 0.584
3.175LeuArg: 3.175 ± 0.476
6.272LeuSer: 6.272 ± 0.731
6.039LeuThr: 6.039 ± 0.689
6.736LeuVal: 6.736 ± 0.542
0.852LeuTrp: 0.852 ± 0.223
2.942LeuTyr: 2.942 ± 0.539
0.0LeuXaa: 0.0 ± 0.0
Met
2.865MetAla: 2.865 ± 0.44
0.31MetCys: 0.31 ± 0.144
1.471MetAsp: 1.471 ± 0.345
1.858MetGlu: 1.858 ± 0.338
1.161MetPhe: 1.161 ± 0.22
1.084MetGly: 1.084 ± 0.241
0.852MetHis: 0.852 ± 0.239
1.161MetIle: 1.161 ± 0.301
2.013MetLys: 2.013 ± 0.374
2.478MetLeu: 2.478 ± 0.42
0.852MetMet: 0.852 ± 0.229
0.774MetAsn: 0.774 ± 0.243
0.697MetPro: 0.697 ± 0.229
1.703MetGln: 1.703 ± 0.279
1.161MetArg: 1.161 ± 0.286
2.4MetSer: 2.4 ± 0.339
2.245MetThr: 2.245 ± 0.393
1.007MetVal: 1.007 ± 0.232
0.387MetTrp: 0.387 ± 0.211
1.394MetTyr: 1.394 ± 0.272
0.0MetXaa: 0.0 ± 0.0
Asn
4.026AsnAla: 4.026 ± 0.592
0.542AsnCys: 0.542 ± 0.216
2.478AsnAsp: 2.478 ± 0.4
2.787AsnGlu: 2.787 ± 0.357
2.013AsnPhe: 2.013 ± 0.408
3.562AsnGly: 3.562 ± 0.509
0.697AsnHis: 0.697 ± 0.227
2.478AsnIle: 2.478 ± 0.435
3.175AsnLys: 3.175 ± 0.382
4.026AsnLeu: 4.026 ± 0.727
2.245AsnMet: 2.245 ± 0.44
2.478AsnAsn: 2.478 ± 0.495
2.323AsnPro: 2.323 ± 0.5
2.091AsnGln: 2.091 ± 0.394
1.626AsnArg: 1.626 ± 0.516
2.323AsnSer: 2.323 ± 0.369
2.865AsnThr: 2.865 ± 0.427
3.02AsnVal: 3.02 ± 0.456
0.542AsnTrp: 0.542 ± 0.221
1.703AsnTyr: 1.703 ± 0.393
0.0AsnXaa: 0.0 ± 0.0
Pro
2.71ProAla: 2.71 ± 0.471
0.31ProCys: 0.31 ± 0.192
2.4ProAsp: 2.4 ± 0.383
2.942ProGlu: 2.942 ± 0.675
1.239ProPhe: 1.239 ± 0.333
2.323ProGly: 2.323 ± 0.407
0.697ProHis: 0.697 ± 0.265
1.626ProIle: 1.626 ± 0.385
2.71ProLys: 2.71 ± 0.452
2.633ProLeu: 2.633 ± 0.442
1.007ProMet: 1.007 ± 0.346
2.633ProAsn: 2.633 ± 0.398
0.619ProPro: 0.619 ± 0.208
1.703ProGln: 1.703 ± 0.354
1.549ProArg: 1.549 ± 0.305
2.323ProSer: 2.323 ± 0.416
1.858ProThr: 1.858 ± 0.432
3.175ProVal: 3.175 ± 0.568
0.31ProTrp: 0.31 ± 0.13
1.316ProTyr: 1.316 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
4.491GlnAla: 4.491 ± 0.563
0.774GlnCys: 0.774 ± 0.236
1.858GlnAsp: 1.858 ± 0.348
3.175GlnGlu: 3.175 ± 0.475
1.626GlnPhe: 1.626 ± 0.334
3.02GlnGly: 3.02 ± 0.457
0.929GlnHis: 0.929 ± 0.27
2.168GlnIle: 2.168 ± 0.368
1.316GlnLys: 1.316 ± 0.289
3.717GlnLeu: 3.717 ± 0.563
1.239GlnMet: 1.239 ± 0.324
2.168GlnAsn: 2.168 ± 0.494
1.394GlnPro: 1.394 ± 0.25
2.323GlnGln: 2.323 ± 0.501
2.245GlnArg: 2.245 ± 0.392
1.626GlnSer: 1.626 ± 0.402
1.239GlnThr: 1.239 ± 0.288
2.71GlnVal: 2.71 ± 0.39
0.929GlnTrp: 0.929 ± 0.308
1.626GlnTyr: 1.626 ± 0.315
0.0GlnXaa: 0.0 ± 0.0
Arg
3.794ArgAla: 3.794 ± 0.647
0.232ArgCys: 0.232 ± 0.189
2.71ArgAsp: 2.71 ± 0.651
2.4ArgGlu: 2.4 ± 0.447
2.245ArgPhe: 2.245 ± 0.475
3.097ArgGly: 3.097 ± 0.398
0.697ArgHis: 0.697 ± 0.223
2.555ArgIle: 2.555 ± 0.524
3.175ArgLys: 3.175 ± 0.529
4.568ArgLeu: 4.568 ± 0.636
1.084ArgMet: 1.084 ± 0.283
1.936ArgAsn: 1.936 ± 0.397
1.936ArgPro: 1.936 ± 0.392
1.394ArgGln: 1.394 ± 0.393
2.013ArgArg: 2.013 ± 0.517
2.942ArgSer: 2.942 ± 0.587
2.245ArgThr: 2.245 ± 0.458
3.484ArgVal: 3.484 ± 0.449
0.542ArgTrp: 0.542 ± 0.268
0.774ArgTyr: 0.774 ± 0.261
0.0ArgXaa: 0.0 ± 0.0
Ser
4.723SerAla: 4.723 ± 0.646
0.387SerCys: 0.387 ± 0.159
4.955SerAsp: 4.955 ± 0.622
3.871SerGlu: 3.871 ± 0.488
2.4SerPhe: 2.4 ± 0.393
6.117SerGly: 6.117 ± 0.806
1.084SerHis: 1.084 ± 0.29
3.329SerIle: 3.329 ± 0.572
4.878SerLys: 4.878 ± 0.619
4.955SerLeu: 4.955 ± 0.56
1.781SerMet: 1.781 ± 0.294
3.252SerAsn: 3.252 ± 0.576
2.091SerPro: 2.091 ± 0.347
2.013SerGln: 2.013 ± 0.393
2.942SerArg: 2.942 ± 0.486
4.646SerSer: 4.646 ± 0.833
3.794SerThr: 3.794 ± 0.691
3.639SerVal: 3.639 ± 0.5
1.161SerTrp: 1.161 ± 0.248
2.478SerTyr: 2.478 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
5.575ThrAla: 5.575 ± 0.765
0.619ThrCys: 0.619 ± 0.228
4.181ThrAsp: 4.181 ± 0.682
3.407ThrGlu: 3.407 ± 0.631
3.252ThrPhe: 3.252 ± 0.523
4.955ThrGly: 4.955 ± 0.715
1.084ThrHis: 1.084 ± 0.271
2.787ThrIle: 2.787 ± 0.439
4.336ThrLys: 4.336 ± 0.593
5.575ThrLeu: 5.575 ± 0.658
1.394ThrMet: 1.394 ± 0.26
2.865ThrAsn: 2.865 ± 0.566
2.4ThrPro: 2.4 ± 0.482
2.091ThrGln: 2.091 ± 0.446
3.562ThrArg: 3.562 ± 0.643
2.633ThrSer: 2.633 ± 0.506
4.336ThrThr: 4.336 ± 0.684
5.033ThrVal: 5.033 ± 0.68
0.387ThrTrp: 0.387 ± 0.143
2.633ThrTyr: 2.633 ± 0.504
0.0ThrXaa: 0.0 ± 0.0
Val
4.568ValAla: 4.568 ± 0.604
0.774ValCys: 0.774 ± 0.227
4.491ValAsp: 4.491 ± 0.653
4.491ValGlu: 4.491 ± 0.562
2.633ValPhe: 2.633 ± 0.482
3.871ValGly: 3.871 ± 0.567
1.007ValHis: 1.007 ± 0.244
3.484ValIle: 3.484 ± 0.574
4.646ValLys: 4.646 ± 0.55
6.349ValLeu: 6.349 ± 0.498
1.626ValMet: 1.626 ± 0.348
3.562ValAsn: 3.562 ± 0.515
2.942ValPro: 2.942 ± 0.5
3.175ValGln: 3.175 ± 0.418
2.555ValArg: 2.555 ± 0.591
5.73ValSer: 5.73 ± 0.684
4.104ValThr: 4.104 ± 0.672
5.497ValVal: 5.497 ± 0.682
0.542ValTrp: 0.542 ± 0.205
2.633ValTyr: 2.633 ± 0.521
0.0ValXaa: 0.0 ± 0.0
Trp
0.465TrpAla: 0.465 ± 0.187
0.155TrpCys: 0.155 ± 0.115
1.007TrpAsp: 1.007 ± 0.34
0.697TrpGlu: 0.697 ± 0.193
0.387TrpPhe: 0.387 ± 0.15
0.774TrpGly: 0.774 ± 0.193
0.31TrpHis: 0.31 ± 0.142
0.387TrpIle: 0.387 ± 0.137
0.852TrpLys: 0.852 ± 0.266
1.549TrpLeu: 1.549 ± 0.234
0.31TrpMet: 0.31 ± 0.193
0.929TrpAsn: 0.929 ± 0.225
0.697TrpPro: 0.697 ± 0.299
0.542TrpGln: 0.542 ± 0.222
0.852TrpArg: 0.852 ± 0.292
1.161TrpSer: 1.161 ± 0.309
0.774TrpThr: 0.774 ± 0.301
1.007TrpVal: 1.007 ± 0.233
0.155TrpTrp: 0.155 ± 0.111
0.232TrpTyr: 0.232 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.942TyrAla: 2.942 ± 0.393
0.852TyrCys: 0.852 ± 0.263
2.4TyrAsp: 2.4 ± 0.275
2.4TyrGlu: 2.4 ± 0.407
1.394TyrPhe: 1.394 ± 0.266
2.633TyrGly: 2.633 ± 0.492
0.774TyrHis: 0.774 ± 0.263
2.245TyrIle: 2.245 ± 0.439
3.02TyrLys: 3.02 ± 0.436
3.717TyrLeu: 3.717 ± 0.557
0.542TyrMet: 0.542 ± 0.193
2.4TyrAsn: 2.4 ± 0.477
1.394TyrPro: 1.394 ± 0.39
1.549TyrGln: 1.549 ± 0.285
1.781TyrArg: 1.781 ± 0.338
2.323TyrSer: 2.323 ± 0.44
2.942TyrThr: 2.942 ± 0.445
2.71TyrVal: 2.71 ± 0.433
0.697TyrTrp: 0.697 ± 0.269
1.858TyrTyr: 1.858 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12916 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski