Hyunsoonleella jejuensis
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2939 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9B3U9|A0A1H9B3U9_9FLAO Exo-beta-1 3-glucanase GH17 family OS=Hyunsoonleella jejuensis OX=419940 GN=SAMN05421824_0444 PE=4 SV=1
MM1 pKa = 7.08 MKK3 pKa = 10.45 KK4 pKa = 8.36 ITLLLLTFLSFSLGFSQTEE23 pKa = 3.99 LLNLSFDD30 pKa = 3.92 AAGSEE35 pKa = 4.95 SVWTPIADD43 pKa = 3.63 AASNPSEE50 pKa = 4.06 VNIAFDD56 pKa = 4.51 ANGNPTGATLLSGINTSDD74 pKa = 3.28 VAGRR78 pKa = 11.84 AYY80 pKa = 9.75 IFRR83 pKa = 11.84 YY84 pKa = 10.17 NNASFDD90 pKa = 3.81 FGTSGSVSISMDD102 pKa = 3.32 VKK104 pKa = 10.49 IDD106 pKa = 3.45 AALTGTNLVFEE117 pKa = 4.58 TQVSKK122 pKa = 11.35 VGGGVVVVTHH132 pKa = 6.56 NNVQNDD138 pKa = 3.68 VAVGGGWIPLTFDD151 pKa = 3.19 MTPNPAEE158 pKa = 4.04 FNNAGTEE165 pKa = 4.17 LYY167 pKa = 10.45 FYY169 pKa = 11.23 FNMAAGAFVGAGGTFLVDD187 pKa = 3.79 NIVVTGGAPAEE198 pKa = 4.17 PTCDD202 pKa = 3.58 DD203 pKa = 3.44 GLMNGDD209 pKa = 3.92 EE210 pKa = 4.38 TGVDD214 pKa = 4.11 CGGPDD219 pKa = 3.58 CSACIEE225 pKa = 4.91 DD226 pKa = 4.17 PTDD229 pKa = 3.83 APSEE233 pKa = 4.01 LASTGTDD240 pKa = 3.26 LYY242 pKa = 11.05 AYY244 pKa = 9.88 SGLSAVGEE252 pKa = 4.07 SDD254 pKa = 4.25 LPGFNFTAFNGGVTISEE271 pKa = 4.3 EE272 pKa = 4.16 TLSGNKK278 pKa = 9.17 VGKK281 pKa = 9.4 LANLDD286 pKa = 3.83 FFGSGWTPIDD296 pKa = 3.2 VTTAYY301 pKa = 9.64 TYY303 pKa = 11.38 VHH305 pKa = 7.04 LDD307 pKa = 3.34 YY308 pKa = 11.13 YY309 pKa = 10.94 AVSGTWFEE317 pKa = 4.67 FFLIDD322 pKa = 4.73 DD323 pKa = 4.38 SLSATICCGSGEE335 pKa = 4.1 EE336 pKa = 3.88 PRR338 pKa = 11.84 YY339 pKa = 10.28 RR340 pKa = 11.84 FGPAPSGQDD349 pKa = 3.13 EE350 pKa = 4.41 PLVLGAWTSVFIPLSDD366 pKa = 3.94 FANYY370 pKa = 9.63 AALTNGTWDD379 pKa = 3.41 GTDD382 pKa = 2.81 IKK384 pKa = 9.51 EE385 pKa = 4.57 TKK387 pKa = 8.89 FTSDD391 pKa = 2.4 GGTIYY396 pKa = 10.65 FDD398 pKa = 3.97 NIYY401 pKa = 10.49 FSTSNVLGVDD411 pKa = 3.83 DD412 pKa = 5.26 FEE414 pKa = 4.61 STSFSVYY421 pKa = 10.28 PNPTQEE427 pKa = 3.43 SWTIKK432 pKa = 10.09 AQNTKK437 pKa = 8.11 ITNIKK442 pKa = 10.18 VYY444 pKa = 10.67 DD445 pKa = 3.78 VLGKK449 pKa = 10.14 NVMMLSPNTIEE460 pKa = 4.17 TKK462 pKa = 10.11 IDD464 pKa = 3.31 GASLMKK470 pKa = 10.58 GIYY473 pKa = 8.37 FAKK476 pKa = 10.07 IEE478 pKa = 4.47 TVSGTSSVKK487 pKa = 10.21 LVKK490 pKa = 10.26 QQ491 pKa = 3.39
Molecular weight: 51.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.694
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.846
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.024
Patrickios 1.341
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A1H9BXK1|A0A1H9BXK1_9FLAO Magnesium transporter MgtE OS=Hyunsoonleella jejuensis OX=419940 GN=SAMN05421824_0742 PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.77 KK27 pKa = 10.58 KK28 pKa = 9.8 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2939
0
2939
1056956
30
3421
359.6
40.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.421 ± 0.04
0.804 ± 0.015
5.809 ± 0.036
6.491 ± 0.048
5.224 ± 0.036
6.418 ± 0.047
1.817 ± 0.022
7.893 ± 0.042
7.766 ± 0.072
9.091 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.109 ± 0.024
6.368 ± 0.048
3.421 ± 0.027
3.194 ± 0.022
3.457 ± 0.031
6.375 ± 0.041
5.862 ± 0.055
6.326 ± 0.033
1.079 ± 0.02
4.076 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here