Pseudomonas phage JBD44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125RNL1|A0A125RNL1_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD44 OX=1777052 GN=JBD44_12 PE=4 SV=1
MM1 pKa = 7.27TKK3 pKa = 8.72EE4 pKa = 3.88TLTIVLFSIGSGLIGYY20 pKa = 9.43AIGIAAAWLGNWFADD35 pKa = 4.62GYY37 pKa = 11.31HH38 pKa = 7.02PLLLSNMVSTPGAEE52 pKa = 4.11DD53 pKa = 3.72GEE55 pKa = 4.62RR56 pKa = 11.84ANEE59 pKa = 3.97YY60 pKa = 9.9PDD62 pKa = 3.87YY63 pKa = 11.08LEE65 pKa = 4.7PPRR68 pKa = 11.84GCFGMCCAGCDD79 pKa = 3.57ARR81 pKa = 11.84SQVNSRR87 pKa = 11.84SKK89 pKa = 9.66TVSEE93 pKa = 4.2QTDD96 pKa = 3.15QSYY99 pKa = 10.97VLNAVLSAQGDD110 pKa = 3.86EE111 pKa = 4.27

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125RNP4|A0A125RNP4_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD44 OX=1777052 GN=JBD44_45 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.1LQAWRR7 pKa = 11.84KK8 pKa = 5.85TAGAAIFGRR17 pKa = 11.84CSRR20 pKa = 11.84KK21 pKa = 9.27AQVVQVLGLVFALAGCAGQVEE42 pKa = 4.72PEE44 pKa = 3.88PRR46 pKa = 11.84TVRR49 pKa = 11.84VEE51 pKa = 4.07VPVAVPCRR59 pKa = 11.84VPAVEE64 pKa = 4.26VPAWATAGLRR74 pKa = 11.84KK75 pKa = 10.01GDD77 pKa = 3.96DD78 pKa = 3.47LQTKK82 pKa = 9.13VRR84 pKa = 11.84ALLAEE89 pKa = 4.0RR90 pKa = 11.84RR91 pKa = 11.84QRR93 pKa = 11.84IGYY96 pKa = 7.6EE97 pKa = 3.99AQLLAANRR105 pKa = 11.84ACQQ108 pKa = 3.1

Molecular weight: 11.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

15342

37

1002

191.8

21.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.459 ± 0.71

1.323 ± 0.12

5.612 ± 0.222

7.053 ± 0.434

3.331 ± 0.278

7.893 ± 0.343

1.636 ± 0.121

5.606 ± 0.271

4.348 ± 0.306

8.428 ± 0.351

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.146

3.409 ± 0.21

4.432 ± 0.273

4.061 ± 0.284

6.798 ± 0.279

5.964 ± 0.309

5.136 ± 0.356

6.362 ± 0.251

1.76 ± 0.174

2.803 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski