Amino acid dipepetide frequency for Pseudomonas phage JBD44

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.644AlaAla: 15.644 ± 2.28
1.76AlaCys: 1.76 ± 0.344
5.997AlaAsp: 5.997 ± 0.717
9.973AlaGlu: 9.973 ± 1.222
3.324AlaPhe: 3.324 ± 0.503
8.213AlaGly: 8.213 ± 0.906
1.63AlaHis: 1.63 ± 0.326
5.476AlaIle: 5.476 ± 0.576
5.867AlaLys: 5.867 ± 0.66
9.908AlaLeu: 9.908 ± 1.117
3.52AlaMet: 3.52 ± 0.434
3.194AlaAsn: 3.194 ± 0.674
3.716AlaPro: 3.716 ± 0.638
5.606AlaGln: 5.606 ± 0.921
7.887AlaArg: 7.887 ± 1.042
5.736AlaSer: 5.736 ± 0.767
5.801AlaThr: 5.801 ± 1.182
6.258AlaVal: 6.258 ± 0.759
2.933AlaTrp: 2.933 ± 0.434
2.151AlaTyr: 2.151 ± 0.356
0.0AlaXaa: 0.0 ± 0.0
Cys
1.304CysAla: 1.304 ± 0.369
0.391CysCys: 0.391 ± 0.205
0.913CysAsp: 0.913 ± 0.234
0.913CysGlu: 0.913 ± 0.222
0.391CysPhe: 0.391 ± 0.173
1.63CysGly: 1.63 ± 0.403
0.326CysHis: 0.326 ± 0.132
0.326CysIle: 0.326 ± 0.126
0.326CysLys: 0.326 ± 0.137
1.173CysLeu: 1.173 ± 0.238
0.326CysMet: 0.326 ± 0.153
0.521CysAsn: 0.521 ± 0.159
0.717CysPro: 0.717 ± 0.249
0.717CysGln: 0.717 ± 0.211
0.782CysArg: 0.782 ± 0.212
0.913CysSer: 0.913 ± 0.252
0.391CysThr: 0.391 ± 0.169
0.391CysVal: 0.391 ± 0.186
0.326CysTrp: 0.326 ± 0.14
0.456CysTyr: 0.456 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.975AspAla: 6.975 ± 0.7
0.652AspCys: 0.652 ± 0.243
2.281AspAsp: 2.281 ± 0.452
3.259AspGlu: 3.259 ± 0.547
1.825AspPhe: 1.825 ± 0.351
5.541AspGly: 5.541 ± 0.788
1.173AspHis: 1.173 ± 0.263
2.999AspIle: 2.999 ± 0.483
2.281AspLys: 2.281 ± 0.394
5.41AspLeu: 5.41 ± 0.612
1.369AspMet: 1.369 ± 0.27
1.63AspAsn: 1.63 ± 0.39
2.347AspPro: 2.347 ± 0.359
2.542AspGln: 2.542 ± 0.593
3.65AspArg: 3.65 ± 0.387
3.064AspSer: 3.064 ± 0.408
2.412AspThr: 2.412 ± 0.427
4.107AspVal: 4.107 ± 0.585
1.434AspTrp: 1.434 ± 0.312
1.108AspTyr: 1.108 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
7.105GluAla: 7.105 ± 1.318
0.782GluCys: 0.782 ± 0.252
3.324GluAsp: 3.324 ± 0.486
4.693GluGlu: 4.693 ± 0.732
3.324GluPhe: 3.324 ± 0.468
5.215GluGly: 5.215 ± 0.731
0.978GluHis: 0.978 ± 0.31
4.367GluIle: 4.367 ± 0.517
2.999GluLys: 2.999 ± 0.483
5.736GluLeu: 5.736 ± 0.758
1.89GluMet: 1.89 ± 0.305
2.477GluAsn: 2.477 ± 0.496
3.129GluPro: 3.129 ± 0.499
4.758GluGln: 4.758 ± 0.54
6.062GluArg: 6.062 ± 0.892
3.194GluSer: 3.194 ± 0.507
2.738GluThr: 2.738 ± 0.385
4.433GluVal: 4.433 ± 0.483
1.63GluTrp: 1.63 ± 0.334
1.695GluTyr: 1.695 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
3.259PheAla: 3.259 ± 0.4
0.456PheCys: 0.456 ± 0.172
2.151PheAsp: 2.151 ± 0.335
2.412PheGlu: 2.412 ± 0.45
1.239PhePhe: 1.239 ± 0.385
3.129PheGly: 3.129 ± 0.395
0.391PheHis: 0.391 ± 0.156
1.76PheIle: 1.76 ± 0.328
1.695PheLys: 1.695 ± 0.348
2.868PheLeu: 2.868 ± 0.386
0.456PheMet: 0.456 ± 0.17
1.63PheAsn: 1.63 ± 0.35
1.434PhePro: 1.434 ± 0.282
0.978PheGln: 0.978 ± 0.219
2.021PheArg: 2.021 ± 0.321
1.825PheSer: 1.825 ± 0.397
2.086PheThr: 2.086 ± 0.349
2.216PheVal: 2.216 ± 0.424
0.456PheTrp: 0.456 ± 0.155
0.847PheTyr: 0.847 ± 0.258
0.0PheXaa: 0.0 ± 0.0
Gly
8.018GlyAla: 8.018 ± 0.839
1.173GlyCys: 1.173 ± 0.338
4.628GlyAsp: 4.628 ± 0.642
5.15GlyGlu: 5.15 ± 0.656
2.477GlyPhe: 2.477 ± 0.414
6.453GlyGly: 6.453 ± 0.731
1.499GlyHis: 1.499 ± 0.271
4.172GlyIle: 4.172 ± 0.612
3.194GlyLys: 3.194 ± 0.435
7.17GlyLeu: 7.17 ± 0.717
2.673GlyMet: 2.673 ± 0.439
2.868GlyAsn: 2.868 ± 0.412
2.412GlyPro: 2.412 ± 0.427
3.129GlyGln: 3.129 ± 0.494
4.758GlyArg: 4.758 ± 0.565
4.302GlySer: 4.302 ± 0.543
4.237GlyThr: 4.237 ± 0.627
6.127GlyVal: 6.127 ± 0.528
1.499GlyTrp: 1.499 ± 0.404
2.999GlyTyr: 2.999 ± 0.472
0.0GlyXaa: 0.0 ± 0.0
His
1.89HisAla: 1.89 ± 0.331
0.261HisCys: 0.261 ± 0.136
1.434HisAsp: 1.434 ± 0.254
1.108HisGlu: 1.108 ± 0.259
0.326HisPhe: 0.326 ± 0.162
1.304HisGly: 1.304 ± 0.292
0.391HisHis: 0.391 ± 0.166
0.587HisIle: 0.587 ± 0.182
0.717HisLys: 0.717 ± 0.209
1.239HisLeu: 1.239 ± 0.261
0.391HisMet: 0.391 ± 0.142
0.391HisAsn: 0.391 ± 0.16
0.978HisPro: 0.978 ± 0.312
0.587HisGln: 0.587 ± 0.191
1.108HisArg: 1.108 ± 0.284
1.304HisSer: 1.304 ± 0.305
0.391HisThr: 0.391 ± 0.129
1.043HisVal: 1.043 ± 0.314
0.326HisTrp: 0.326 ± 0.167
0.587HisTyr: 0.587 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
6.91IleAla: 6.91 ± 0.641
0.456IleCys: 0.456 ± 0.198
3.911IleAsp: 3.911 ± 0.552
3.716IleGlu: 3.716 ± 0.379
1.304IlePhe: 1.304 ± 0.337
4.563IleGly: 4.563 ± 0.6
1.043IleHis: 1.043 ± 0.2
2.347IleIle: 2.347 ± 0.426
2.542IleLys: 2.542 ± 0.585
4.498IleLeu: 4.498 ± 0.742
1.043IleMet: 1.043 ± 0.267
2.151IleAsn: 2.151 ± 0.388
2.738IlePro: 2.738 ± 0.464
2.216IleGln: 2.216 ± 0.376
3.52IleArg: 3.52 ± 0.458
3.781IleSer: 3.781 ± 0.517
3.194IleThr: 3.194 ± 0.43
3.455IleVal: 3.455 ± 0.532
0.978IleTrp: 0.978 ± 0.262
1.695IleTyr: 1.695 ± 0.397
0.0IleXaa: 0.0 ± 0.0
Lys
5.019LysAla: 5.019 ± 0.705
0.652LysCys: 0.652 ± 0.213
1.564LysAsp: 1.564 ± 0.305
3.324LysGlu: 3.324 ± 0.599
1.239LysPhe: 1.239 ± 0.305
2.999LysGly: 2.999 ± 0.408
1.108LysHis: 1.108 ± 0.319
2.281LysIle: 2.281 ± 0.391
2.281LysLys: 2.281 ± 0.445
4.172LysLeu: 4.172 ± 0.507
0.847LysMet: 0.847 ± 0.203
1.369LysAsn: 1.369 ± 0.317
2.151LysPro: 2.151 ± 0.459
1.304LysGln: 1.304 ± 0.302
3.455LysArg: 3.455 ± 0.477
2.542LysSer: 2.542 ± 0.42
2.477LysThr: 2.477 ± 0.37
2.412LysVal: 2.412 ± 0.415
0.717LysTrp: 0.717 ± 0.244
1.564LysTyr: 1.564 ± 0.311
0.0LysXaa: 0.0 ± 0.0
Leu
9.908LeuAla: 9.908 ± 1.013
0.847LeuCys: 0.847 ± 0.327
5.671LeuAsp: 5.671 ± 0.688
5.215LeuGlu: 5.215 ± 0.635
2.607LeuPhe: 2.607 ± 0.526
6.062LeuGly: 6.062 ± 0.615
1.108LeuHis: 1.108 ± 0.274
5.15LeuIle: 5.15 ± 0.68
3.911LeuLys: 3.911 ± 0.685
6.518LeuLeu: 6.518 ± 0.679
1.825LeuMet: 1.825 ± 0.357
4.107LeuAsn: 4.107 ± 0.581
4.172LeuPro: 4.172 ± 0.604
2.673LeuGln: 2.673 ± 0.344
6.388LeuArg: 6.388 ± 0.574
5.019LeuSer: 5.019 ± 0.613
4.628LeuThr: 4.628 ± 0.704
4.824LeuVal: 4.824 ± 0.54
1.043LeuTrp: 1.043 ± 0.241
2.607LeuTyr: 2.607 ± 0.362
0.0LeuXaa: 0.0 ± 0.0
Met
3.194MetAla: 3.194 ± 0.512
0.326MetCys: 0.326 ± 0.137
1.108MetAsp: 1.108 ± 0.273
1.89MetGlu: 1.89 ± 0.319
0.587MetPhe: 0.587 ± 0.189
0.913MetGly: 0.913 ± 0.291
0.326MetHis: 0.326 ± 0.167
1.956MetIle: 1.956 ± 0.41
1.304MetLys: 1.304 ± 0.361
1.956MetLeu: 1.956 ± 0.393
0.391MetMet: 0.391 ± 0.141
1.173MetAsn: 1.173 ± 0.285
1.434MetPro: 1.434 ± 0.279
0.913MetGln: 0.913 ± 0.269
1.499MetArg: 1.499 ± 0.282
2.738MetSer: 2.738 ± 0.498
2.086MetThr: 2.086 ± 0.357
1.173MetVal: 1.173 ± 0.311
0.261MetTrp: 0.261 ± 0.103
0.652MetTyr: 0.652 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.455AsnAla: 3.455 ± 0.589
0.521AsnCys: 0.521 ± 0.168
1.825AsnAsp: 1.825 ± 0.353
2.347AsnGlu: 2.347 ± 0.495
1.239AsnPhe: 1.239 ± 0.27
3.129AsnGly: 3.129 ± 0.689
0.652AsnHis: 0.652 ± 0.184
1.76AsnIle: 1.76 ± 0.342
1.108AsnLys: 1.108 ± 0.274
3.39AsnLeu: 3.39 ± 0.503
0.521AsnMet: 0.521 ± 0.174
1.108AsnAsn: 1.108 ± 0.314
1.825AsnPro: 1.825 ± 0.297
1.239AsnGln: 1.239 ± 0.231
2.086AsnArg: 2.086 ± 0.353
2.673AsnSer: 2.673 ± 0.405
1.434AsnThr: 1.434 ± 0.276
1.825AsnVal: 1.825 ± 0.313
0.717AsnTrp: 0.717 ± 0.212
1.239AsnTyr: 1.239 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
3.976ProAla: 3.976 ± 0.568
0.652ProCys: 0.652 ± 0.244
3.39ProAsp: 3.39 ± 0.601
4.107ProGlu: 4.107 ± 0.538
1.173ProPhe: 1.173 ± 0.326
4.172ProGly: 4.172 ± 0.762
0.521ProHis: 0.521 ± 0.16
2.281ProIle: 2.281 ± 0.392
2.021ProLys: 2.021 ± 0.434
4.498ProLeu: 4.498 ± 0.481
1.173ProMet: 1.173 ± 0.301
1.108ProAsn: 1.108 ± 0.297
1.499ProPro: 1.499 ± 0.345
1.304ProGln: 1.304 ± 0.291
2.151ProArg: 2.151 ± 0.422
2.738ProSer: 2.738 ± 0.397
3.064ProThr: 3.064 ± 0.458
2.412ProVal: 2.412 ± 0.375
0.587ProTrp: 0.587 ± 0.202
1.499ProTyr: 1.499 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
5.28GlnAla: 5.28 ± 1.011
0.847GlnCys: 0.847 ± 0.264
1.564GlnAsp: 1.564 ± 0.365
2.803GlnGlu: 2.803 ± 0.387
1.108GlnPhe: 1.108 ± 0.289
3.52GlnGly: 3.52 ± 0.52
0.587GlnHis: 0.587 ± 0.246
2.216GlnIle: 2.216 ± 0.431
1.043GlnLys: 1.043 ± 0.244
3.65GlnLeu: 3.65 ± 0.606
1.108GlnMet: 1.108 ± 0.297
0.782GlnAsn: 0.782 ± 0.257
1.564GlnPro: 1.564 ± 0.369
1.825GlnGln: 1.825 ± 0.318
2.803GlnArg: 2.803 ± 0.5
1.695GlnSer: 1.695 ± 0.336
2.477GlnThr: 2.477 ± 0.395
3.846GlnVal: 3.846 ± 0.509
0.456GlnTrp: 0.456 ± 0.197
1.434GlnTyr: 1.434 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
7.17ArgAla: 7.17 ± 0.773
0.913ArgCys: 0.913 ± 0.227
3.846ArgAsp: 3.846 ± 0.512
5.801ArgGlu: 5.801 ± 1.05
2.412ArgPhe: 2.412 ± 0.357
5.019ArgGly: 5.019 ± 0.738
1.695ArgHis: 1.695 ± 0.306
4.498ArgIle: 4.498 ± 0.589
3.259ArgLys: 3.259 ± 0.569
5.28ArgLeu: 5.28 ± 0.507
1.89ArgMet: 1.89 ± 0.413
1.956ArgAsn: 1.956 ± 0.385
2.999ArgPro: 2.999 ± 0.477
2.738ArgGln: 2.738 ± 0.451
5.15ArgArg: 5.15 ± 0.703
3.194ArgSer: 3.194 ± 0.454
2.412ArgThr: 2.412 ± 0.416
4.172ArgVal: 4.172 ± 0.625
1.239ArgTrp: 1.239 ± 0.278
2.347ArgTyr: 2.347 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
7.04SerAla: 7.04 ± 0.759
0.652SerCys: 0.652 ± 0.25
2.999SerAsp: 2.999 ± 0.375
3.65SerGlu: 3.65 ± 0.555
2.281SerPhe: 2.281 ± 0.454
5.215SerGly: 5.215 ± 0.558
0.456SerHis: 0.456 ± 0.182
4.563SerIle: 4.563 ± 0.67
2.803SerLys: 2.803 ± 0.353
4.498SerLeu: 4.498 ± 0.578
2.216SerMet: 2.216 ± 0.335
2.086SerAsn: 2.086 ± 0.378
3.064SerPro: 3.064 ± 0.633
2.738SerGln: 2.738 ± 0.396
3.259SerArg: 3.259 ± 0.479
3.129SerSer: 3.129 ± 0.537
2.933SerThr: 2.933 ± 0.413
4.041SerVal: 4.041 ± 0.523
0.652SerTrp: 0.652 ± 0.176
1.369SerTyr: 1.369 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
7.366ThrAla: 7.366 ± 0.768
0.391ThrCys: 0.391 ± 0.171
2.542ThrAsp: 2.542 ± 0.487
3.194ThrGlu: 3.194 ± 0.288
2.542ThrPhe: 2.542 ± 0.389
3.781ThrGly: 3.781 ± 0.643
0.847ThrHis: 0.847 ± 0.299
3.324ThrIle: 3.324 ± 0.424
2.021ThrLys: 2.021 ± 0.41
3.194ThrLeu: 3.194 ± 0.379
1.369ThrMet: 1.369 ± 0.335
1.239ThrAsn: 1.239 ± 0.454
2.281ThrPro: 2.281 ± 0.374
2.151ThrGln: 2.151 ± 0.354
2.607ThrArg: 2.607 ± 0.494
3.455ThrSer: 3.455 ± 0.616
2.738ThrThr: 2.738 ± 0.521
4.237ThrVal: 4.237 ± 0.817
0.847ThrTrp: 0.847 ± 0.241
1.239ThrTyr: 1.239 ± 0.244
0.0ThrXaa: 0.0 ± 0.0
Val
6.258ValAla: 6.258 ± 0.78
0.717ValCys: 0.717 ± 0.243
3.716ValAsp: 3.716 ± 0.464
4.563ValGlu: 4.563 ± 0.484
2.151ValPhe: 2.151 ± 0.344
5.28ValGly: 5.28 ± 0.583
0.913ValHis: 0.913 ± 0.227
3.65ValIle: 3.65 ± 0.536
2.607ValLys: 2.607 ± 0.445
5.476ValLeu: 5.476 ± 0.588
1.239ValMet: 1.239 ± 0.288
2.477ValAsn: 2.477 ± 0.484
3.716ValPro: 3.716 ± 0.597
1.956ValGln: 1.956 ± 0.403
4.302ValArg: 4.302 ± 0.526
4.954ValSer: 4.954 ± 0.574
3.585ValThr: 3.585 ± 0.654
4.107ValVal: 4.107 ± 0.496
0.587ValTrp: 0.587 ± 0.188
1.369ValTyr: 1.369 ± 0.29
0.0ValXaa: 0.0 ± 0.0
Trp
1.89TrpAla: 1.89 ± 0.474
0.196TrpCys: 0.196 ± 0.114
1.499TrpAsp: 1.499 ± 0.372
0.847TrpGlu: 0.847 ± 0.216
0.847TrpPhe: 0.847 ± 0.239
0.456TrpGly: 0.456 ± 0.158
0.326TrpHis: 0.326 ± 0.138
0.978TrpIle: 0.978 ± 0.387
0.782TrpLys: 0.782 ± 0.211
1.956TrpLeu: 1.956 ± 0.41
0.587TrpMet: 0.587 ± 0.188
0.913TrpAsn: 0.913 ± 0.218
0.652TrpPro: 0.652 ± 0.257
0.456TrpGln: 0.456 ± 0.192
1.564TrpArg: 1.564 ± 0.31
0.978TrpSer: 0.978 ± 0.265
0.782TrpThr: 0.782 ± 0.257
1.304TrpVal: 1.304 ± 0.351
0.261TrpTrp: 0.261 ± 0.141
0.326TrpTyr: 0.326 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.933TyrAla: 2.933 ± 0.459
0.587TyrCys: 0.587 ± 0.202
1.76TyrAsp: 1.76 ± 0.304
1.564TyrGlu: 1.564 ± 0.311
0.913TyrPhe: 0.913 ± 0.356
2.347TyrGly: 2.347 ± 0.382
0.391TyrHis: 0.391 ± 0.186
1.369TyrIle: 1.369 ± 0.275
0.717TyrLys: 0.717 ± 0.229
1.695TyrLeu: 1.695 ± 0.367
0.978TyrMet: 0.978 ± 0.261
0.913TyrAsn: 0.913 ± 0.284
1.499TyrPro: 1.499 ± 0.408
0.782TyrGln: 0.782 ± 0.234
2.933TyrArg: 2.933 ± 0.508
2.412TyrSer: 2.412 ± 0.443
1.499TyrThr: 1.499 ± 0.301
1.239TyrVal: 1.239 ± 0.302
0.652TyrTrp: 0.652 ± 0.2
0.847TyrTyr: 0.847 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15342 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski