Klebsiella phage YMC16/01/N133_KPN_BP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A248XD53|A0A248XD53_9CAUD Uncharacterized protein OS=Klebsiella phage YMC16/01/N133_KPN_BP OX=2026102 GN=KPNN133_021 PE=4 SV=1
MM1 pKa = 8.05AITPTQPTPGLAQYY15 pKa = 10.67EE16 pKa = 4.43SDD18 pKa = 3.59TVGGLVEE25 pKa = 5.06LWAGDD30 pKa = 3.51TPAPVTISGKK40 pKa = 10.4YY41 pKa = 9.46SATLEE46 pKa = 4.2AAGIPANTPVSLDD59 pKa = 3.6YY60 pKa = 9.78EE61 pKa = 4.45TGDD64 pKa = 3.02IALVDD69 pKa = 3.74GTTVTKK75 pKa = 10.92ANAITVGALLPRR87 pKa = 11.84AGGTGGSMAVYY98 pKa = 10.31KK99 pKa = 10.61AGCVNIKK106 pKa = 10.08ALNWPASFDD115 pKa = 3.52TDD117 pKa = 3.57AKK119 pKa = 10.7KK120 pKa = 10.41LAAFDD125 pKa = 4.11LAEE128 pKa = 4.03CQIYY132 pKa = 10.74VKK134 pKa = 10.67VPYY137 pKa = 10.47YY138 pKa = 10.74SS139 pKa = 3.13

Molecular weight: 14.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248XD80|A0A248XD80_9CAUD Uncharacterized protein OS=Klebsiella phage YMC16/01/N133_KPN_BP OX=2026102 GN=KPNN133_044 PE=4 SV=1
MM1 pKa = 7.17FKK3 pKa = 10.09VHH5 pKa = 7.3KK6 pKa = 10.16FRR8 pKa = 11.84LLLLRR13 pKa = 11.84VAIHH17 pKa = 6.75PKK19 pKa = 7.09TPRR22 pKa = 11.84WARR25 pKa = 11.84RR26 pKa = 11.84VVMWAGLRR34 pKa = 11.84MITSRR39 pKa = 11.84YY40 pKa = 8.79ARR42 pKa = 11.84SPNGARR48 pKa = 11.84RR49 pKa = 3.42

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

17511

37

1490

250.2

27.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.359 ± 0.766

1.022 ± 0.117

6.836 ± 0.262

5.813 ± 0.288

3.055 ± 0.173

7.618 ± 0.28

1.553 ± 0.164

4.905 ± 0.178

5.083 ± 0.382

8.874 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.112

3.866 ± 0.213

4.688 ± 0.327

3.632 ± 0.347

6.133 ± 0.288

5.979 ± 0.303

6.088 ± 0.319

6.938 ± 0.241

2.033 ± 0.17

3.055 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski