Chiqui virus
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4N706|A0A2Z4N706_9REOV P7 OS=Chiqui virus OX=2250219 GN=P7 PE=4 SV=1
MM1 pKa = 7.56 ALIDD5 pKa = 4.08 SEE7 pKa = 4.75 ANQIRR12 pKa = 11.84 IKK14 pKa = 9.92 GANWQRR20 pKa = 11.84 ITEE23 pKa = 4.29 KK24 pKa = 10.86 ARR26 pKa = 11.84 LDD28 pKa = 3.6 YY29 pKa = 10.19 TQLSTILAQHH39 pKa = 6.43 SSIQTASIIPIPEE52 pKa = 4.14 DD53 pKa = 2.97 QKK55 pKa = 10.17 QTVKK59 pKa = 11.12 NIVTVTDD66 pKa = 3.62 TSGSADD72 pKa = 3.56 NLNDD76 pKa = 4.15 LLYY79 pKa = 10.79 QLFIGSSKK87 pKa = 10.21 PLEE90 pKa = 3.85 IAKK93 pKa = 10.32 KK94 pKa = 9.14 IFRR97 pKa = 11.84 IAATIVTQEE106 pKa = 3.84 NSIITTNGYY115 pKa = 9.8 FYY117 pKa = 10.88 RR118 pKa = 11.84 DD119 pKa = 3.68 AASHH123 pKa = 5.38 PMIRR127 pKa = 11.84 QLEE130 pKa = 4.32 IIEE133 pKa = 4.46 RR134 pKa = 11.84 NDD136 pKa = 4.2 AINLFSKK143 pKa = 9.85 YY144 pKa = 8.84 YY145 pKa = 10.3 RR146 pKa = 11.84 WMEE149 pKa = 3.79 LLLSNEE155 pKa = 4.16 PLLVSDD161 pKa = 4.12 QDD163 pKa = 3.72 VGALLNVINVPTTVDD178 pKa = 3.52 GQVVSDD184 pKa = 3.54 SRR186 pKa = 11.84 YY187 pKa = 8.52 VVSDD191 pKa = 2.86 VMFPNGHH198 pKa = 6.76 ISTTIDD204 pKa = 3.19 EE205 pKa = 4.57 NLLRR209 pKa = 11.84 TQIQDD214 pKa = 3.29 MPLFGYY220 pKa = 10.42 FSDD223 pKa = 5.15 IITDD227 pKa = 3.42 NFNFGTHH234 pKa = 5.51 RR235 pKa = 11.84 QHH237 pKa = 8.14 LDD239 pKa = 3.05 VLRR242 pKa = 11.84 LVLIYY247 pKa = 10.73 AFRR250 pKa = 11.84 RR251 pKa = 11.84 WFTNAGLVTSGIQIGALSTFNRR273 pKa = 11.84 QNALHH278 pKa = 6.66 NGDD281 pKa = 4.08 DD282 pKa = 3.81 TDD284 pKa = 3.91 IEE286 pKa = 4.76 SKK288 pKa = 11.0 GRR290 pKa = 11.84 ILPSLFLTALLPSEE304 pKa = 4.9 LLRR307 pKa = 11.84 KK308 pKa = 9.25 VKK310 pKa = 10.6 NAGGEE315 pKa = 4.28 TNLDD319 pKa = 3.78 EE320 pKa = 5.9 IIADD324 pKa = 3.7 NLEE327 pKa = 4.02 EE328 pKa = 4.06 LMSRR332 pKa = 11.84 IGKK335 pKa = 10.02 LSVNTPTKK343 pKa = 9.25 ITQSALDD350 pKa = 3.34 KK351 pKa = 10.52 MFNGLYY357 pKa = 10.11 KK358 pKa = 10.38 ISDD361 pKa = 3.77 FGGKK365 pKa = 9.03 NLSQGYY371 pKa = 7.92 EE372 pKa = 4.01 HH373 pKa = 7.56 ASKK376 pKa = 10.81 QSIRR380 pKa = 11.84 FSDD383 pKa = 3.71 YY384 pKa = 10.67 TKK386 pKa = 10.23 MGFSKK391 pKa = 10.82 VAGLALTDD399 pKa = 3.94 SGSATLPTKK408 pKa = 10.26 DD409 pKa = 4.72 APDD412 pKa = 3.94 PSIGEE417 pKa = 3.9 YY418 pKa = 9.05 YY419 pKa = 10.54 ARR421 pKa = 11.84 HH422 pKa = 5.06 KK423 pKa = 9.96 TEE425 pKa = 4.18 LLQLIANEE433 pKa = 3.98 RR434 pKa = 11.84 LSVDD438 pKa = 3.45 EE439 pKa = 4.86 LSVIFGGKK447 pKa = 9.91 LISTDD452 pKa = 3.05 QKK454 pKa = 10.99 LNQIFKK460 pKa = 10.16 TMLMPSNTVTLVDD473 pKa = 3.61 SSKK476 pKa = 11.05 VSFPTLINQCITQNGIDD493 pKa = 4.89 KK494 pKa = 10.59 LPKK497 pKa = 9.9 DD498 pKa = 4.25 PKK500 pKa = 10.88 VDD502 pKa = 3.12 IPTLIRR508 pKa = 11.84 TNWDD512 pKa = 2.57 ISAEE516 pKa = 4.27 TISEE520 pKa = 4.44 LEE522 pKa = 3.98 LSDD525 pKa = 3.62 VGVEE529 pKa = 4.06 GGYY532 pKa = 10.66 DD533 pKa = 3.42 YY534 pKa = 11.96 SKK536 pKa = 11.05 LFKK539 pKa = 10.57 NKK541 pKa = 9.41 PFTGYY546 pKa = 10.63 DD547 pKa = 3.85 FSQGGIKK554 pKa = 10.45 LGTFGTTWYY563 pKa = 8.72 TDD565 pKa = 3.02 EE566 pKa = 4.83 GVRR569 pKa = 11.84 QVALSNEE576 pKa = 4.33 VVGADD581 pKa = 3.17 MLMARR586 pKa = 11.84 MLDD589 pKa = 3.63 KK590 pKa = 10.63 RR591 pKa = 11.84 WNLPSEE597 pKa = 4.51 PGVQRR602 pKa = 11.84 TMLEE606 pKa = 3.89 MMKK609 pKa = 10.91 DD610 pKa = 3.55 DD611 pKa = 4.03 MASEE615 pKa = 4.46 NPTLPTILAEE625 pKa = 4.17 GQIVFLWSQFATIMEE640 pKa = 4.58 HH641 pKa = 7.84 DD642 pKa = 4.84 DD643 pKa = 3.53 WNDD646 pKa = 3.46 DD647 pKa = 4.79 DD648 pKa = 6.61 VIQEE652 pKa = 4.1 WKK654 pKa = 10.69 DD655 pKa = 3.19 KK656 pKa = 11.09 GGSLGDD662 pKa = 3.48 HH663 pKa = 6.48 VNNIFEE669 pKa = 4.35 VAIPMLSKK677 pKa = 11.2 AKK679 pKa = 9.32 MSTVGDD685 pKa = 3.75 HH686 pKa = 6.93 FSRR689 pKa = 11.84 TIRR692 pKa = 11.84 DD693 pKa = 3.29 TDD695 pKa = 3.12 GTYY698 pKa = 10.97 AKK700 pKa = 9.73 EE701 pKa = 3.79 VSYY704 pKa = 11.26 NILDD708 pKa = 5.6 LITHH712 pKa = 6.29 GTSQIEE718 pKa = 4.41 GGSTFQQFIATEE730 pKa = 4.28 PEE732 pKa = 4.45 LGDD735 pKa = 3.02 QGYY738 pKa = 10.35 NGRR741 pKa = 11.84 NLYY744 pKa = 10.6 AYY746 pKa = 7.56 FTGLVNDD753 pKa = 5.49 NIPIDD758 pKa = 4.68 KK759 pKa = 8.11 ITKK762 pKa = 9.81 VITPAKK768 pKa = 9.35 LRR770 pKa = 11.84 SIVVSDD776 pKa = 3.58 STTVSIGDD784 pKa = 3.54 NSEE787 pKa = 4.14 PLHH790 pKa = 6.59 KK791 pKa = 10.69 VLEE794 pKa = 4.27 SQIISDD800 pKa = 3.54 NTTEE804 pKa = 4.13 FNN806 pKa = 3.71
Molecular weight: 89.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.881
IPC2_protein 4.838
IPC_protein 4.825
Toseland 4.685
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.685
Rodwell 4.685
Grimsley 4.596
Solomon 4.8
Lehninger 4.749
Nozaki 4.902
DTASelect 5.118
Thurlkill 4.698
EMBOSS 4.711
Sillero 4.965
Patrickios 4.177
IPC_peptide 4.8
IPC2_peptide 4.952
IPC2.peptide.svr19 4.955
Protein with the highest isoelectric point:
>tr|A0A2Z4N706|A0A2Z4N706_9REOV P7 OS=Chiqui virus OX=2250219 GN=P7 PE=4 SV=1
MM1 pKa = 7.75 DD2 pKa = 4.72 TLTGRR7 pKa = 11.84 ITNDD11 pKa = 2.47 SSAQEE16 pKa = 4.8 DD17 pKa = 3.76 LQIWPKK23 pKa = 9.21 TLQNTRR29 pKa = 11.84 EE30 pKa = 3.88 ITYY33 pKa = 7.79 TQQDD37 pKa = 3.38 NQKK40 pKa = 8.26 QQGRR44 pKa = 11.84 KK45 pKa = 5.55 VQKK48 pKa = 9.68 SVKK51 pKa = 6.89 THH53 pKa = 5.9 SYY55 pKa = 8.88 MLSTNEE61 pKa = 3.59 VLQLHH66 pKa = 6.17 EE67 pKa = 4.54 MLIKK71 pKa = 10.01 IYY73 pKa = 10.12 NRR75 pKa = 11.84 HH76 pKa = 6.42 KK77 pKa = 10.48 GTLPNPKK84 pKa = 10.16 AIANLLNSQPVKK96 pKa = 10.69 MMIEE100 pKa = 4.35 SKK102 pKa = 11.03 VKK104 pKa = 10.83 LSDD107 pKa = 3.46 DD108 pKa = 3.42 QKK110 pKa = 11.52 RR111 pKa = 11.84 NLTEE115 pKa = 3.74 IVTNTLANWTYY126 pKa = 11.37 DD127 pKa = 3.3 ADD129 pKa = 3.89 HH130 pKa = 6.96 FNMNKK135 pKa = 8.0 QLRR138 pKa = 11.84 RR139 pKa = 11.84 AILNKK144 pKa = 9.5 HH145 pKa = 6.18 DD146 pKa = 4.66 LSILVMDD153 pKa = 5.0 NDD155 pKa = 4.66 DD156 pKa = 4.39 LQSATQTEE164 pKa = 4.38 NTVTSITMALTIEE177 pKa = 4.28 EE178 pKa = 4.44 VAFLSNYY185 pKa = 10.11 GKK187 pKa = 10.83 GKK189 pKa = 10.12 SCFVLYY195 pKa = 9.53 TFNGPRR201 pKa = 11.84 FAKK204 pKa = 10.15 YY205 pKa = 8.79 IHH207 pKa = 6.7 PVYY210 pKa = 10.11 TILSQVVPTISLSDD224 pKa = 3.65 VKK226 pKa = 11.13 NSLIDD231 pKa = 3.61 TQHH234 pKa = 6.95 PYY236 pKa = 10.05 YY237 pKa = 10.34 KK238 pKa = 10.66 ARR240 pKa = 11.84 FRR242 pKa = 11.84 TLLTFDD248 pKa = 4.65 EE249 pKa = 5.23 FNDD252 pKa = 3.47 NQSNYY257 pKa = 9.77 RR258 pKa = 11.84 KK259 pKa = 9.45 VDD261 pKa = 3.21 VVIITNSSEE270 pKa = 3.98 NKK272 pKa = 9.8 QKK274 pKa = 10.57 VSSYY278 pKa = 7.37 TRR280 pKa = 11.84 KK281 pKa = 9.52 GWKK284 pKa = 9.62 FSQNKK289 pKa = 5.81 QTTFYY294 pKa = 8.89 WTMNGLTYY302 pKa = 10.61 AGYY305 pKa = 9.25 PLYY308 pKa = 10.64 FMTKK312 pKa = 8.28 ITPSDD317 pKa = 3.55 YY318 pKa = 11.02 RR319 pKa = 11.84 DD320 pKa = 3.21 ACVSYY325 pKa = 10.68 GATGLVNNFKK335 pKa = 10.02 IHH337 pKa = 6.34 PNFYY341 pKa = 10.9 LLLNSYY347 pKa = 10.31 CRR349 pKa = 11.84 INFKK353 pKa = 10.53 PIVSALTRR361 pKa = 11.84 SRR363 pKa = 11.84 ISYY366 pKa = 9.71 ISNKK370 pKa = 8.23 MPVMPTEE377 pKa = 4.7 DD378 pKa = 2.95 MSAYY382 pKa = 9.73 TKK384 pKa = 8.16 MTMWLRR390 pKa = 11.84 EE391 pKa = 3.54 VDD393 pKa = 4.18 RR394 pKa = 11.84 IHH396 pKa = 7.09 NGTEE400 pKa = 3.83 SKK402 pKa = 10.58 LRR404 pKa = 11.84 NSSIIIIADD413 pKa = 3.27 KK414 pKa = 11.16 GSGKK418 pKa = 6.58 TTCTKK423 pKa = 10.8 YY424 pKa = 10.92 LLQQLRR430 pKa = 11.84 DD431 pKa = 3.69 TSPSSKK437 pKa = 8.3 TWGRR441 pKa = 11.84 VDD443 pKa = 3.52 SDD445 pKa = 5.29 AFGKK449 pKa = 9.29 WLSKK453 pKa = 9.74 RR454 pKa = 11.84 RR455 pKa = 11.84 SMGMRR460 pKa = 11.84 STDD463 pKa = 2.82 ILLSNWQEE471 pKa = 3.7 LDD473 pKa = 3.12 ALQNDD478 pKa = 4.17 EE479 pKa = 5.5 SIPTYY484 pKa = 9.81 FAHH487 pKa = 7.26 EE488 pKa = 4.36 MEE490 pKa = 4.74 QMLLSAGMVEE500 pKa = 4.43 YY501 pKa = 10.76 GFDD504 pKa = 3.73 PLSEE508 pKa = 4.08 LRR510 pKa = 11.84 ILDD513 pKa = 3.5 EE514 pKa = 4.54 FRR516 pKa = 11.84 PIIYY520 pKa = 9.0 TIINDD525 pKa = 3.8 EE526 pKa = 4.41 EE527 pKa = 3.95 EE528 pKa = 4.29 GLAAFYY534 pKa = 11.43 NEE536 pKa = 4.0 LDD538 pKa = 3.7 KK539 pKa = 11.76 FEE541 pKa = 5.47 DD542 pKa = 3.94 LPIGLMLEE550 pKa = 3.97 SHH552 pKa = 6.79 TDD554 pKa = 3.43 VEE556 pKa = 4.23 ISKK559 pKa = 9.3 MFGTGHH565 pKa = 6.98 IVTLDD570 pKa = 3.23 APYY573 pKa = 10.33 NAEE576 pKa = 3.75 AAVADD581 pKa = 4.78 RR582 pKa = 11.84 KK583 pKa = 10.16 RR584 pKa = 11.84 SKK586 pKa = 10.5 ISINVEE592 pKa = 3.64 LMLFRR597 pKa = 11.84 AWSRR601 pKa = 11.84 LRR603 pKa = 11.84 VSTYY607 pKa = 10.91 PMLRR611 pKa = 11.84 TQAFFDD617 pKa = 3.89 TQLLAIASDD626 pKa = 3.67 QEE628 pKa = 4.4 TNPRR632 pKa = 3.56
Molecular weight: 72.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.648
IPC2_protein 7.922
IPC_protein 7.834
Toseland 8.141
ProMoST 8.419
Dawson 8.639
Bjellqvist 8.624
Wikipedia 8.726
Rodwell 8.712
Grimsley 8.302
Solomon 8.741
Lehninger 8.712
Nozaki 8.653
DTASelect 8.521
Thurlkill 8.595
EMBOSS 8.799
Sillero 8.799
Patrickios 4.38
IPC_peptide 8.741
IPC2_peptide 7.366
IPC2.peptide.svr19 7.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
7971
329
1301
885.7
100.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.319 ± 0.367
1.104 ± 0.281
6.197 ± 0.24
6.122 ± 0.283
4.215 ± 0.187
5.018 ± 0.263
1.882 ± 0.122
7.916 ± 0.171
5.721 ± 0.248
8.744 ± 0.403
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.785 ± 0.241
5.545 ± 0.315
4.391 ± 0.433
4.303 ± 0.27
5.131 ± 0.216
7.377 ± 0.393
7.327 ± 0.297
5.633 ± 0.259
1.28 ± 0.161
3.989 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here