Oxalobacteraceae bacterium CAVE-383
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3601 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1QP88|A0A4V1QP88_9BURK Uncharacterized protein OS=Oxalobacteraceae bacterium CAVE-383 OX=2478672 GN=D9O50_16250 PE=4 SV=1
MM1 pKa = 7.99 PDD3 pKa = 3.09 ANGDD7 pKa = 3.65 FVAPVAKK14 pKa = 10.26 SAIEE18 pKa = 3.88 KK19 pKa = 9.2 VQVVDD24 pKa = 3.26 VDD26 pKa = 3.73 MVLQLKK32 pKa = 10.46 DD33 pKa = 3.42 GTSMVLAGGAISAMDD48 pKa = 3.94 ASHH51 pKa = 6.73 TEE53 pKa = 4.04 VKK55 pKa = 10.66 FSDD58 pKa = 3.75 GLQGLSKK65 pKa = 10.86 LFDD68 pKa = 3.95 LVGTIAIPKK77 pKa = 9.21 LDD79 pKa = 5.54 PILSSLQGHH88 pKa = 6.81 PNSEE92 pKa = 4.23 GQGAGANANQPAATEE107 pKa = 4.38 SAGLTASVTSQIVNQLTQIVQANAQSVTIKK137 pKa = 10.05 TVDD140 pKa = 3.46 PSAKK144 pKa = 10.04 VAVLSQSTKK153 pKa = 10.14 TGDD156 pKa = 3.31 VDD158 pKa = 4.27 SQPLQHH164 pKa = 6.68 TEE166 pKa = 3.75 PPIPPEE172 pKa = 4.05 RR173 pKa = 11.84 PGVIPQEE180 pKa = 4.48 HH181 pKa = 7.27 IIGPDD186 pKa = 3.41 APAMALSLVNLTTITQIGNVLYY208 pKa = 10.84 GSGGTPASATDD219 pKa = 3.67 AGNNAQFASQVINAPANATEE239 pKa = 3.89 IHH241 pKa = 6.62 AGANIPTNDD250 pKa = 3.85 FIKK253 pKa = 10.95 VVDD256 pKa = 3.52 ITLTGNGIAQSIEE269 pKa = 4.05 IKK271 pKa = 10.46 GVPAGMSIANGTDD284 pKa = 2.73 LGGGVWSIPVVSGQRR299 pKa = 11.84 DD300 pKa = 3.3 YY301 pKa = 11.54 AIQIEE306 pKa = 5.01 YY307 pKa = 7.51 TTVPANPQTPIHH319 pKa = 5.6 QQFSMEE325 pKa = 4.16 FDD327 pKa = 3.5 VTMTTADD334 pKa = 4.13 GIVNLSQVKK343 pKa = 9.12 QFVVKK348 pKa = 10.48 DD349 pKa = 3.75 VNSANDD355 pKa = 3.48 LTFIDD360 pKa = 4.33 STTGEE365 pKa = 4.33 SVYY368 pKa = 10.87 VLPAQGNSHH377 pKa = 6.54 IVHH380 pKa = 6.81 AGDD383 pKa = 4.06 AGVTIIGSNANDD395 pKa = 3.67 FLFGGAGADD404 pKa = 3.93 TINGGTGNTYY414 pKa = 10.9 LEE416 pKa = 4.67 GGGGADD422 pKa = 3.76 HH423 pKa = 7.24 LNGGVGGINTAGYY436 pKa = 7.45 TLSATGVTVDD446 pKa = 4.39 LVAGTGTGGDD456 pKa = 3.64 AQGDD460 pKa = 4.14 TLTNIQNVIGSTHH473 pKa = 6.43 NDD475 pKa = 3.12 IIVASAAANNINGGGHH491 pKa = 6.9 DD492 pKa = 4.75 SIGGDD497 pKa = 3.41 TVSYY501 pKa = 10.05 AGSGTGVIIDD511 pKa = 4.34 LAAGTGTGGNAAGDD525 pKa = 4.1 KK526 pKa = 9.93 LTGIQNVIGSDD537 pKa = 3.47 HH538 pKa = 7.18 NDD540 pKa = 3.01 TFFGSSEE547 pKa = 3.84 GNAFDD552 pKa = 5.82 GGLHH556 pKa = 6.82 DD557 pKa = 5.95 LNGANTVSYY566 pKa = 11.49 ANDD569 pKa = 3.56 TSGTGAIVNLATGHH583 pKa = 6.25 GSGAAAGDD591 pKa = 3.93 SYY593 pKa = 12.19 VNIQNIIGTAFNDD606 pKa = 3.84 TFIDD610 pKa = 4.04 GAGANRR616 pKa = 11.84 YY617 pKa = 9.71 DD618 pKa = 3.92 GGVGGNDD625 pKa = 2.9 TVDD628 pKa = 3.44 YY629 pKa = 10.15 SNAGAGVTVNFVTGHH644 pKa = 5.87 GAGDD648 pKa = 3.42 IAEE651 pKa = 4.72 GDD653 pKa = 3.71 TFVNVQNVIGSNFDD667 pKa = 3.73 DD668 pKa = 4.35 VFTAGIDD675 pKa = 3.59 SKK677 pKa = 11.45 NFNGGAGFDD686 pKa = 3.58 TVRR689 pKa = 11.84 YY690 pKa = 9.62 DD691 pKa = 3.3 SAASGIIIDD700 pKa = 5.32 AINNVGRR707 pKa = 11.84 GAAAGNTYY715 pKa = 9.99 TGIEE719 pKa = 4.12 AFVGGGSDD727 pKa = 4.4 DD728 pKa = 3.81 MFIASADD735 pKa = 3.53 ANSFNGGAAGSDD747 pKa = 3.17 TVDD750 pKa = 3.4 YY751 pKa = 10.71 SNSNAAVTVDD761 pKa = 3.59 LFFTRR766 pKa = 11.84 GQGTSGGYY774 pKa = 9.59 AQGDD778 pKa = 3.9 VLVNIANVIGSAGDD792 pKa = 3.93 DD793 pKa = 3.38 LFLANSVQNSFEE805 pKa = 4.25 GGGGNNTVSYY815 pKa = 9.08 EE816 pKa = 4.11 HH817 pKa = 7.01 SSSADD822 pKa = 3.29 STGVIVDD829 pKa = 4.43 LSATDD834 pKa = 4.09 GSGTSGNYY842 pKa = 10.37 ALGDD846 pKa = 3.56 KK847 pKa = 10.41 FSNIQNVIGSAFNDD861 pKa = 3.61 TFVASSVANVFTGGGGIDD879 pKa = 3.3 TVSYY883 pKa = 10.93 AKK885 pKa = 9.38 STSADD890 pKa = 2.89 GVTGVTVDD898 pKa = 3.98 LSTVDD903 pKa = 3.54 GLGGAGHH910 pKa = 6.28 GHH912 pKa = 6.3 GNYY915 pKa = 10.31 AEE917 pKa = 5.05 GDD919 pKa = 3.87 TIINVQNAIGSIYY932 pKa = 10.42 NDD934 pKa = 3.62 TFIGGAVANTFTGGGGSDD952 pKa = 3.24 TVSYY956 pKa = 10.73 AGSTAVTVDD965 pKa = 3.53 LVNGVGVGGNAEE977 pKa = 3.76 GDD979 pKa = 3.59 KK980 pKa = 10.83 YY981 pKa = 11.43 INVQNVIGGDD991 pKa = 3.6 ADD993 pKa = 3.73 DD994 pKa = 4.96 TIIDD998 pKa = 3.7 NLAANTIDD1006 pKa = 3.67 GGTVSLHH1013 pKa = 5.73 NRR1015 pKa = 11.84 VSYY1018 pKa = 11.03 ANSVSSPGVAAGVTVDD1034 pKa = 3.87 LTATDD1039 pKa = 3.9 GTGTSGGYY1047 pKa = 8.32 ATGDD1051 pKa = 3.44 KK1052 pKa = 10.67 LSNIQDD1058 pKa = 3.95 LTGSAADD1065 pKa = 3.74 DD1066 pKa = 3.78 TFVASLAANNLDD1078 pKa = 4.01 GGASTAASHH1087 pKa = 6.53 NRR1089 pKa = 11.84 VSYY1092 pKa = 10.64 DD1093 pKa = 3.05 KK1094 pKa = 11.33 SVDD1097 pKa = 3.36 GSGAAAGVTVDD1108 pKa = 4.14 LVNGVGTGGYY1118 pKa = 9.85 AQGDD1122 pKa = 4.23 TYY1124 pKa = 12.03 ANIQDD1129 pKa = 3.94 VTGSKK1134 pKa = 10.71 ADD1136 pKa = 3.85 DD1137 pKa = 4.89 LIIDD1141 pKa = 4.02 SAAANTIDD1149 pKa = 3.89 GGTGSLHH1156 pKa = 5.46 NTVSYY1161 pKa = 11.04 ANSVTSTGAAAGVTINLNLADD1182 pKa = 4.09 GSGNSGGYY1190 pKa = 10.1 AVGDD1194 pKa = 3.6 KK1195 pKa = 10.84 LSNIQNLVGSQADD1208 pKa = 3.68 DD1209 pKa = 3.69 TFVASAAANFLDD1221 pKa = 4.29 GGTGSLHH1228 pKa = 6.2 NRR1230 pKa = 11.84 VDD1232 pKa = 3.65 YY1233 pKa = 10.82 SASVDD1238 pKa = 3.44 AGNAAAAVTVDD1249 pKa = 3.51 LSGNTGTGGYY1259 pKa = 9.86 AQGDD1263 pKa = 4.23 TYY1265 pKa = 12.03 ANIQDD1270 pKa = 3.99 VTGSGADD1277 pKa = 3.58 DD1278 pKa = 4.44 LFIASTAANNFDD1290 pKa = 4.1 GGVSTAASHH1299 pKa = 6.26 NRR1301 pKa = 11.84 VSYY1304 pKa = 9.14 SQSGTGVVVDD1314 pKa = 5.11 LSVTNGNGTSGGAAGDD1330 pKa = 3.81 TFVNIQDD1337 pKa = 3.54 ITGSNQNDD1345 pKa = 3.68 TFVASTAANNFDD1357 pKa = 4.2 GGTGSDD1363 pKa = 3.23 TVSYY1367 pKa = 11.16 ARR1369 pKa = 11.84 VTGGVGVTVDD1379 pKa = 3.79 LTAGGALTGGAAAGDD1394 pKa = 4.25 TYY1396 pKa = 11.4 TSIEE1400 pKa = 4.1 NVIGSANNDD1409 pKa = 3.42 LFIDD1413 pKa = 4.27 GGGAVPNAYY1422 pKa = 9.04 TGGGGVDD1429 pKa = 3.98 RR1430 pKa = 11.84 ISYY1433 pKa = 9.98 DD1434 pKa = 3.13 NSAAGVNVDD1443 pKa = 5.32 FGTGLNMGTGTGSYY1457 pKa = 10.27 AQGDD1461 pKa = 4.55 TYY1463 pKa = 11.68 VGIQNVTGSKK1473 pKa = 10.41 FDD1475 pKa = 4.8 DD1476 pKa = 3.83 NFTASTTVSAATANAFDD1493 pKa = 4.71 GNTGTLHH1500 pKa = 6.6 NRR1502 pKa = 11.84 VLYY1505 pKa = 9.57 TGTGTGVTVNLSNIIVNGVAAGSGGGGAAGDD1536 pKa = 4.07 TYY1538 pKa = 11.97 ANIQDD1543 pKa = 3.64 ITGTTSDD1550 pKa = 3.94 DD1551 pKa = 4.06 RR1552 pKa = 11.84 IIASAAANNIDD1563 pKa = 3.84 GGTGSLHH1570 pKa = 6.23 NRR1572 pKa = 11.84 VDD1574 pKa = 3.61 YY1575 pKa = 8.84 TASVDD1580 pKa = 3.98 AGNAPAGVTVDD1591 pKa = 3.81 LSGNTGTGGYY1601 pKa = 9.86 AQGDD1605 pKa = 4.23 TYY1607 pKa = 12.03 ANIQDD1612 pKa = 3.99 VTGSGADD1619 pKa = 3.54 DD1620 pKa = 4.48 LFIASAAANNFDD1632 pKa = 4.37 GGVSTAASHH1641 pKa = 6.35 NRR1643 pKa = 11.84 VSYY1646 pKa = 9.8 LQSATGVVVDD1656 pKa = 5.88 LSVTDD1661 pKa = 3.86 GTGTSGGAAGDD1672 pKa = 3.81 TFVNIQDD1679 pKa = 3.54 ITGSNQNDD1687 pKa = 3.68 TFVASTAANNFDD1699 pKa = 4.2 GGTGSDD1705 pKa = 3.23 TVSYY1709 pKa = 11.16 ARR1711 pKa = 11.84 VTGGVGVTVDD1721 pKa = 3.79 LTAGGALTGGAAAGDD1736 pKa = 4.25 TYY1738 pKa = 11.4 TSIEE1742 pKa = 4.1 NVIGSANNDD1751 pKa = 3.42 LFIDD1755 pKa = 4.27 GGGAVPNAYY1764 pKa = 9.04 TGGGGVDD1771 pKa = 3.98 RR1772 pKa = 11.84 ISYY1775 pKa = 9.98 DD1776 pKa = 3.13 NSAAGVNVDD1785 pKa = 5.32 FGTGLNMGTGTGSYY1799 pKa = 10.27 AQGDD1803 pKa = 4.55 TYY1805 pKa = 11.68 VGIQNVTGSKK1815 pKa = 10.41 FDD1817 pKa = 4.8 DD1818 pKa = 3.83 NFTASTTVSAATANAFDD1835 pKa = 4.71 GNTGTLHH1842 pKa = 6.6 NRR1844 pKa = 11.84 VLYY1847 pKa = 9.57 TGTGTGVTVNLSNIIVNGVAAGSGGGGAAGDD1878 pKa = 4.07 TYY1880 pKa = 11.97 ANIQDD1885 pKa = 3.63 ITGTASDD1892 pKa = 3.94 DD1893 pKa = 4.08 TIVASSAANNIDD1905 pKa = 3.59 GGASTAASHH1914 pKa = 6.51 NRR1916 pKa = 11.84 VDD1918 pKa = 3.81 YY1919 pKa = 10.74 SASVDD1924 pKa = 3.41 AGNVAVGVTVDD1935 pKa = 4.22 LLNSVGTGGYY1945 pKa = 9.91 AQGDD1949 pKa = 4.23 TYY1951 pKa = 12.03 ANIQDD1956 pKa = 3.87 VTGSGAADD1964 pKa = 3.44 TFVASAAANKK1974 pKa = 9.8 FDD1976 pKa = 4.37 GGAGSDD1982 pKa = 3.46 TVDD1985 pKa = 3.43 YY1986 pKa = 9.08 STSTAIIVNLSALTQNTLAAGTGSGGLAAGDD2017 pKa = 3.55 TYY2019 pKa = 11.62 TNIEE2023 pKa = 4.31 NIKK2026 pKa = 10.44 GGAGNDD2032 pKa = 3.23 LFYY2035 pKa = 11.24 AGTAANAFTGGGGIDD2050 pKa = 3.37 TVSYY2054 pKa = 10.7 VSSTNGVTASLIANAVGSGGDD2075 pKa = 3.34 AAGDD2079 pKa = 3.73 TFSGITNLTGSAQNDD2094 pKa = 4.13 TLTGLAAGGSTLNGGAGSDD2113 pKa = 3.72 TLNATGDD2120 pKa = 3.55 NNIFIGGAGGDD2131 pKa = 3.56 TMTGNGGLNNTADD2144 pKa = 3.99 YY2145 pKa = 8.94 TGSGAVVVDD2154 pKa = 4.7 LFFTDD2159 pKa = 3.84 GTGTSGGDD2167 pKa = 3.18 ATGDD2171 pKa = 3.54 KK2172 pKa = 10.91 LSGIQNVKK2180 pKa = 10.54 GSGGNDD2186 pKa = 2.75 TFFANSVANVFTGNGGTDD2204 pKa = 3.22 TVSYY2208 pKa = 10.21 SHH2210 pKa = 6.41 STTTVIASLLAGVGGTSGDD2229 pKa = 3.71 ATGDD2233 pKa = 3.45 TYY2235 pKa = 11.84 NAITNLTGGSGDD2247 pKa = 3.71 DD2248 pKa = 3.55 TLTGLLAGGSTLVGNAGNDD2267 pKa = 3.21 RR2268 pKa = 11.84 LIATGGNNVLMGGAGADD2285 pKa = 3.39 NMSATGANNTASYY2298 pKa = 10.77 AGSTNGNIIVDD2309 pKa = 4.08 LTKK2312 pKa = 10.46 TDD2314 pKa = 3.48 GTGTSGGDD2322 pKa = 3.13 AAGDD2326 pKa = 3.51 ILSGIQNVIGGLGDD2340 pKa = 3.87 DD2341 pKa = 5.44 KK2342 pKa = 11.46 FILDD2346 pKa = 4.01 HH2347 pKa = 6.38 NANIIDD2353 pKa = 4.39 GGNGSNTVSYY2363 pKa = 10.38 EE2364 pKa = 3.75 HH2365 pKa = 6.86 EE2366 pKa = 4.04 TTINVTVDD2374 pKa = 3.23 LSGTGIANGDD2384 pKa = 4.08 GLFGAQDD2391 pKa = 3.71 TLTNIQNAIGGGGNDD2406 pKa = 3.55 IFLAGNAHH2414 pKa = 6.11 NFFDD2418 pKa = 4.65 GGGGVDD2424 pKa = 3.42 TVSYY2428 pKa = 11.06 ANNNGAHH2435 pKa = 6.39 GAAIAANTAGVTVDD2449 pKa = 4.01 FHH2451 pKa = 8.68 GNIGSGNWAAGDD2463 pKa = 3.57 TYY2465 pKa = 11.41 TNIEE2469 pKa = 4.18 NVIGTTGNDD2478 pKa = 3.28 TFIDD2482 pKa = 3.89 AGDD2485 pKa = 4.12 GVHH2488 pKa = 7.04 NIYY2491 pKa = 10.97 NGGANGAGSNDD2502 pKa = 3.25 TVSYY2506 pKa = 10.51 QFSATGGALTLSIDD2520 pKa = 3.68 GTHH2523 pKa = 6.83 ANAGNAAGDD2532 pKa = 3.79 VFTNIANLTGSDD2544 pKa = 3.65 TVNSEE2549 pKa = 3.55 LWGDD2553 pKa = 3.65 GNANILTAMGSTNTNILHH2571 pKa = 6.24 GNGGGAGTDD2580 pKa = 3.47 VLDD2583 pKa = 4.56 GRR2585 pKa = 11.84 LGGHH2589 pKa = 5.58 NTLIAGDD2596 pKa = 3.93 TGSTTVDD2603 pKa = 2.95 INASADD2609 pKa = 3.51 PNHH2612 pKa = 7.02 AGGSILGTNIDD2623 pKa = 4.02 QIVGSTTGAVTVKK2636 pKa = 9.84 MFNLTTALDD2645 pKa = 3.52 ISSFSATATVGGPKK2659 pKa = 9.71 VSHH2662 pKa = 6.31 ITTLDD2667 pKa = 3.38 LSGDD2671 pKa = 3.79 GVGTQISISANDD2683 pKa = 3.79 VIALGNNHH2691 pKa = 5.69 QLSVRR2696 pKa = 11.84 MDD2698 pKa = 3.69 GNDD2701 pKa = 3.85 SINLVLQAGEE2711 pKa = 4.02 HH2712 pKa = 6.03 SVLFGNGDD2720 pKa = 3.66 YY2721 pKa = 11.57 AFFDD2725 pKa = 3.99 ANNVEE2730 pKa = 4.59 LARR2733 pKa = 11.84 IHH2735 pKa = 6.33 QLTT2738 pKa = 3.51
Molecular weight: 267.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.617
IPC_protein 3.706
Toseland 3.439
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.706
Rodwell 3.516
Grimsley 3.35
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.177
Thurlkill 3.503
EMBOSS 3.706
Sillero 3.834
Patrickios 0.884
IPC_peptide 3.706
IPC2_peptide 3.795
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A4V1QQH5|A0A4V1QQH5_9BURK Extracellular solute-binding protein OS=Oxalobacteraceae bacterium CAVE-383 OX=2478672 GN=D9O50_02575 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3601
0
3601
1228013
29
6384
341.0
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.389 ± 0.059
0.885 ± 0.014
5.499 ± 0.039
5.149 ± 0.034
3.701 ± 0.024
8.015 ± 0.053
2.179 ± 0.019
5.544 ± 0.029
3.948 ± 0.036
10.449 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.022
3.246 ± 0.042
4.915 ± 0.04
3.948 ± 0.027
6.178 ± 0.052
5.638 ± 0.032
5.008 ± 0.059
6.916 ± 0.033
1.228 ± 0.015
2.484 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here