Xanthomonas virus phiXaf18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Naesvirus; unclassified Naesvirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PQM4|A0A5P8PQM4_9CAUD Uncharacterized protein OS=Xanthomonas virus phiXaf18 OX=2653651 GN=phiXaf18_63 PE=4 SV=1
MM1 pKa = 7.63PAATLLPTEE10 pKa = 4.69DD11 pKa = 3.14QTFQALARR19 pKa = 11.84WVGKK23 pKa = 10.56VLDD26 pKa = 4.63LPSNTLQIVKK36 pKa = 10.03GFQNLTATPTGSYY49 pKa = 9.75VVISPGTMQRR59 pKa = 11.84QDD61 pKa = 2.99FGRR64 pKa = 11.84RR65 pKa = 11.84YY66 pKa = 9.63YY67 pKa = 11.15DD68 pKa = 3.73PDD70 pKa = 3.19NSQAVQQAHH79 pKa = 5.79LTYY82 pKa = 10.24SYY84 pKa = 11.12QVDD87 pKa = 4.12CYY89 pKa = 11.03GPNGPTWASVLSVAWRR105 pKa = 11.84SMWGVDD111 pKa = 3.84NMAPPILTPLYY122 pKa = 10.84ADD124 pKa = 5.1APQQLNIVNAQGQYY138 pKa = 6.88EE139 pKa = 4.22QRR141 pKa = 11.84FMIRR145 pKa = 11.84LFGQVNQDD153 pKa = 2.63VGLPQDD159 pKa = 3.71FFEE162 pKa = 5.23QIQLDD167 pKa = 4.05SFIVADD173 pKa = 3.82QLPP176 pKa = 3.6

Molecular weight: 19.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PRX6|A0A5P8PRX6_9CAUD Phage_lysozyme2 domain-containing protein OS=Xanthomonas virus phiXaf18 OX=2653651 GN=phiXaf18_47 PE=4 SV=1
MM1 pKa = 7.37CRR3 pKa = 11.84KK4 pKa = 8.33PQAIRR9 pKa = 11.84RR10 pKa = 11.84LSTTQAHH17 pKa = 5.93RR18 pKa = 11.84RR19 pKa = 11.84AVLLLCQAVAVCALLVLCAEE39 pKa = 4.78TIGTYY44 pKa = 10.32VDD46 pKa = 3.09MARR49 pKa = 11.84RR50 pKa = 11.84VLL52 pKa = 3.75

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14294

36

827

213.3

23.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.054 ± 0.498

1.168 ± 0.174

5.954 ± 0.263

4.778 ± 0.285

3.057 ± 0.168

8.031 ± 0.576

1.847 ± 0.159

4.24 ± 0.2

3.029 ± 0.261

8.423 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.16

3.295 ± 0.176

5.744 ± 0.273

4.512 ± 0.253

6.709 ± 0.297

5.786 ± 0.215

6.443 ± 0.388

7.115 ± 0.228

1.854 ± 0.126

2.616 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski